LOCUS       RC240046                8514 bp    DNA     circular  SYN 
DEFINITION  SMC1A (myc-DDK-tagged) - Human structural maintenance of chromosomes 1A (SMC1A), transcript variant 2
ACCESSION   NM_001281463
VERSION     NM_001281463.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4671..4762 
                     /label="Myc-DDK"  
     misc_feature    4792..5377 
                     /label="PolyA signal"  
     misc_feature    4849..4868 
                     /label="Restriction Enzyme"  
     misc_feature    5653..6325 
                     /label="ColE1"  
     misc_feature    6755..7549 
                     /label="Kan/Neo"  
     misc_feature    7584..7886 
                     /label="SV40 ori"  
     misc_feature    7972..8449 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4653..4659 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4653 
                     /label="SMC1A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTGAGGTG TCGATCCCCA CCCCCCACCG CTATGTCAGA GGTAAGTCAA 
     1081 ATCTCATGGA TGCCATCAGC TTTGTGCTAG GTGAAAAAAC CAGCAACCTG CGGGTAAAGA 
     1141 CCCTGCGGGA CCTGATCCAT GGAGCTCCTG TGGGCAAGCC AGCTGCCAAC CGGGCCTTTG 
     1201 TCAGCATGGT CTACTCTGAG GAGGGTGCTG AGGACCGTAC CTTTGCCCGT GTCATTGTAG 
     1261 GAGGTTCTTC TGAGTACAAG ATCAACAACA AAGTGGTCCA ACTACATGAG TACAGTGAGG 
     1321 AATTAGAGAA GTTGGGCATT CTCATCAAAG CTCGTAACTT CCTCGTTTTC CAGGGTGCTG 
     1381 TGGAATCTAT TGCCATGAAG AACCCCAAAG AGAGGACAGC TCTATTTGAA GAGATTAGTC 
     1441 GTTCTGGGGA GCTGGCGCAG GAGTATGACA AGCGAAAGAA GGAAATGGTG AAGGCTGAAG 
     1501 AGGACACACA GTTTAATTAC CATCGCAAGA AAAATATTGC GGCTGAACGC AAGGAAGCAA 
     1561 AGCAGGAGAA AGAAGAGGCT GACCGGTACC AGCGCCTGAA GGATGAGGTA GTACGGGCTC 
     1621 AGGTACAGCT GCAGCTCTTT AAGCTTTACC ATAATGAAGT GGAAATTGAG AAGCTCAACA 
     1681 AGGAACTGGC CTCAAAGAAC AAGGAGATCG AGAAGGACAA GAAGCGTATG GACAAGGTGG 
     1741 AGGATGAACT GAAGGAGAAG AAGAAGGAGC TGGGCAAAAT GATGCGGGAG CAGCAGCAGA 
     1801 TTGAGAAGGA GATCAAGGAG AAGGACTCAG AATTGAACCA GAAGCGGCCT CAGTACATCA 
     1861 AAGCCAAGGA GAACACCTCC CACAAAATCA AGAAGCTGGA AGCAGCCAAG AAGTCTCTGC 
     1921 AGAATGCTCA GAAGCACTAC AAGAAGCGTA AAGGTGACAT GGATGAGCTG GAGAAGGAGA 
     1981 TGCTGTCAGT GGAGAAGGCT CGGCAGGAGT TTGAAGAACG GATGGAAGAA GAGAGTCAGA 
     2041 GTCAGGGCAG AGATTTGACG TTGGAGGAGA ATCAGGTGAA GAAATACCAC CGGTTGAAAG 
     2101 AAGAAGCCAG CAAGAGAGCA GCTACCCTGG CCCAGGAGCT GGAGAAATTC AATCGAGACC 
     2161 AGAAAGCTGA CCAGGACCGT CTGGATCTGG AAGAACGGAA GAAAGTAGAG ACAGAGGCCA 
     2221 AGATCAAGCA AAAGCTGCGG GAAATTGAAG AGAATCAGAA GCGGATTGAG AAACTGGAGG 
     2281 AATACATCAC CACTAGCAAG CAGTCCCTAG AAGAGCAGAA GAAGCTAGAG GGGGAGCTGA 
     2341 CAGAGGAGGT GGAGATGGCC AAGCGGCGTA TTGATGAAAT CAATAAGGAG CTGAACCAGG 
     2401 TGATGGAGCA GCTAGGGGAT GCCCGCATCG ACCGCCAGGA GAGCAGCCGC CAGCAGCGAA 
     2461 AGGCAGAGAT AATGGAAAGC ATCAAGCGCC TTTACCCTGG CTCTGTGTAC GGCCGCCTCA 
     2521 TTGACCTATG CCAGCCCACA CAAAAGAAGT ATCAGATTGC TGTAACCAAG GTTTTGGGCA 
     2581 AGAACATGGA TGCCATTATT GTGGACTCGG AGAAGACAGG CCGGGACTGT ATTCAGTATA 
     2641 TCAAGGAGCA GCGTGGGGAG CCTGAGACCT TCTTGCCTCT TGACTACCTG GAGGTGAAGC 
     2701 CTACAGATGA GAAACTCCGG GAGCTGAAGG GGGCCAAGCT AGTGATTGAT GTGATTCGCT 
     2761 ATGAGCCACC TCATATCAAA AAGGCCCTGC AGTATGCTTG TGGCAATGCC CTTGTCTGTG 
     2821 ACAACGTGGA AGATGCCCGC CGCATTGCCT TTGGAGGCCA CCAGCGCCAC AAGACAGTGG 
     2881 CACTGGATGG AACCCTATTC CAGAAGTCAG GAGTGATCTC TGGTGGGGCC AGTGACCTGA 
     2941 AGGCCAAGGC ACGGCGCTGG GATGAGAAAG CAGTAGACAA GTTGAAAGAG AAGAAGGAGC 
     3001 GCTTGACAGA GGAGCTGAAA GAGCAGATGA AGGCAAAACG GAAAGAGGCA GAGCTGCGTC 
     3061 AGGTGCAGTC TCAGGCCCAT GGACTGCAGA TGCGGCTCAA GTACTCCCAG AGTGACCTAG 
     3121 AACAGACCAA GACACGACAT CTAGCCCTGA ATCTGCAGGA AAAATCCAAG CTGGAGAGTG 
     3181 AGCTAGCCAA CTTTGGGCCT CGCATTAATG ATATCAAGAG GATCATTCAG AGCCGAGAGA 
     3241 GGGAAATGAA AGACTTGAAG GAGAAGATGA ACCAGGTAGA GGATGAGGTG TTTGAAGAGT 
     3301 TTTGTCGGGA GATTGGTGTG CGCAACATCC GGGAGTTTGA GGAAGAAAAG GTGAAACGGC 
     3361 AGAATGAAAT CGCCAAGAAG CGTTTGGAGT TTGAGAATCA GAAGACTCGC TTGGGCATTC 
     3421 AGTTGGATTT TGAAAAGAAC CAACTGAAGG AGGACCAAGA TAAAGTACAC ATGTGGGAGC 
     3481 AGACAGTGAA AAAAGATGAA AATGAGATAG AAAAGCTCAA AAAGGAGGAA CAAAGACACA 
     3541 TGAAGATCAT AGATGAGACC ATGGCTCAGC TACAAGACCT GAAGAATCAG CATCTGGCCA 
     3601 AGAAGTCGGA AGTGAATGAC AAGAATCATG AGATGGAGGA GATTCGTAAG AAACTCGGGG 
     3661 GCGCCAACAA GGAAATGACC CATTTACAGA AGGAGGTGAC AGCCATTGAG ACCAAGCTTG 
     3721 AACAGAAGCG CAGTGACCGT CACAACTTGC TACAGGCCTG TAAGATGCAG GACATTAAGT 
     3781 TGCCACTGTC AAAAGGCACC ATGGATGATA TTAGTCAGGA AGAGGGTAGC TCCCAGGGGG 
     3841 AGGACTCAGT GAGTGGTTCA CAGAGAATTT CCAGTATCTA TGCACGAGAG GCCCTCATTG 
     3901 AGATTGACTA CGGTGATCTG TGTGAGGATC TGAAGGATGC CCAGGCTGAG GAAGAGATCA 
     3961 AGCAAGAGAT GAACACACTG CAGCAGAAGC TGAATGAGCA GCAGAGTGTG CTTCAGCGTA 
     4021 TTGCCGCCCC CAACATGAAG GCCATGGAAA AGCTGGAAAG TGTCCGAGAC AAGTTCCAGG 
     4081 AGACCTCAGA TGAGTTTGAA GCAGCCCGAA AGCGAGCAAA GAAGGCCAAG CAGGCATTCG 
     4141 AACAGATCAA GAAGGAGCGC TTTGACCGCT TCAATGCTTG TTTTGAATCT GTGGCTACCA 
     4201 ACATTGATGA GATCTATAAG GCCCTGTCCC GCAATAGCAG TGCCCAGGCA TTCCTGGGCC 
     4261 CTGAGAACCC TGAAGAGCCC TACTTGGATG GCATCAACTA CAACTGTGTG GCTCCTGGGA 
     4321 AACGCTTCCG GCCTATGGAC AACTTGTCAG GCGGGGAGAA GACAGTGGCA GCTCTGGCCC 
     4381 TGCTCTTTGC CATCCACAGC TACAAGCCAG CCCCCTTCTT CGTCCTGGAT GAGATTGATG 
     4441 CTGCCTTGGA TAACACCAAC ATTGGCAAGG TGGCAAATTA CATCAAGGAG CAGTCGACTT 
     4501 GCAACTTCCA GGCCATCGTC ATCTCTCTCA AGGAGGAGTT CTACACCAAG GCCGAGAGCC 
     4561 TCATTGGAGT CTATCCTGAG CAAGGGGACT GTGTGATCAG CAAAGTCCTG ACCTTCGACC 
     4621 TCACCAAGTA CCCAGATGCC AACCCCAACC CCAATGAGCA GACGCGTACG CGGCCGCTCG 
     4681 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4741 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4801 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4861 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4921 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4981 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5041 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5101 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5161 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5221 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5281 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5341 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5401 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5461 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5521 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5581 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5641 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5701 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5761 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5821 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5881 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5941 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6001 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6061 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6121 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6181 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6241 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6301 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6361 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6421 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6481 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6541 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6601 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6661 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6721 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6781 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6841 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6901 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6961 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7021 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7081 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7141 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7201 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7261 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7321 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7381 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7441 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7501 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7561 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7621 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7681 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7741 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7921 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7981 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8041 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8101 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8161 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8221 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8281 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8341 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8401 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8461 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//