LOCUS       RC240045                8511 bp    DNA     circular  SYN 
DEFINITION  PER3 (myc-DDK-tagged) - Human period circadian clock 3 (PER3), transcript variant 1
ACCESSION   NM_001289862
VERSION     NM_001289862.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="PER3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCGGG GAAGCTCCTG GCCCCGGGAG ACGGGGGGCT AAGGACGAGG 
     1081 CCCTGGGCGA AGAATCGGGG GAGCGGTGGA GCCCCGAGTT CCATCTGCAG AGGAAATTGG 
     1141 CGGACAGCAG CCACAGTGAA CAGCAAGATC GAAACAGAGT TTCTGAAGAA CTTATCATGG 
     1201 TTGTCCAAGA AATGAAAAAA TACTTCCCCT CGGAGAGACG CAATAAACCA AGCACTCTAG 
     1261 ATGCCCTCAA CTATGCTCTC CGCTGTGTCC ACAGCGTTCA AGCAAACAGT GAGTTTTTCC 
     1321 AGATTCTCAG TCAGAATGGA GCACCTCAGG CAGATGTGAG CATGTACAGT CTTGAGGAGC 
     1381 TGGCCACTAT CGCTTCAGAA CACACTTCCA AAAACACAGA TACCTTTGTG GCAGTATTTT 
     1441 CATTTCTGTC TGGAAGGTTA GTGCACATTT CTGAACAGGC TGCTTTGATC CTGAATCGTA 
     1501 AGAAAGATGT CCTGGCGTCT TCTCACTTTG TTGACCTGCT TGCACCTCAA GACATGAGGG 
     1561 TATTCTACGC GCACACTGCC AGAGCTCAGC TTCCTTTCTG GAACAACTGG ACCCAAAGAG 
     1621 CAGCTGCACG GTATGAATGT GCTCCGGTGA AACCTTTTTT CTGCAGGATC CGTGGAGGTG 
     1681 AAGACAGAAA GCAAGAGAAG TGTCACTCCC CATTCCGGAT CATCCCCTAT CTGATTCATG 
     1741 TACATCACCC TGCCCAGCCA GAATTGGAAT CGGAACCTTG CTGTCTCACT GTGGTTGAAA 
     1801 AGATTCACTC TGGTTATGAA GCTCCTCGGA TCCCAGTGAA TAAAAGAATC TTCACCACCA 
     1861 CACACACCCC AGGGTGTGTT TTTCTTGAAG TAGATGAAAA AGCAGTGCCT TTGCTGGGTT 
     1921 ACCTACCTCA GGACCTGATT GGAACATCGA TCCTAAGCTA CCTGCACCCT GAAGATCGTT 
     1981 CTCTGATGGT TGCCATACAC CAAAAAGTTT TGAAGTATGC AGGGCATCCT CCCTTTGAAC 
     2041 ATTCTCCCAT TCGATTTTGT ACTCAAAACG GAGACTACAT CATACTGGAT TCCAGTTGGT 
     2101 CCAGCTTTGT GAATCCCTGG AGCCGGAAGA TTTCTTTCAT CATTGGTCGG CATAAAGTTC 
     2161 GAACGAGCCC ACTAAATGAG GATGTTTTTG CTACCAAAAT TAAAAAGATG AACGATAATG 
     2221 ACAAAGACAT AACAGAATTA CAAGAACAAA TTTACAAACT TCTCTTACAG CCAGTTCACG 
     2281 TGAGCGTGTC CAGCGGCTAC GGGAGCCTGG GGAGCAGCGG GTCGCAGGAG CAGCTTGTCA 
     2341 GCATCGCCTC CTCCAGTGAG GCCAGTGGGC ACCGTGTGGA GGAGACGAAG GCGGAGCAGA 
     2401 TGACCTTGCA GCAGGTCTAT GCCAGTGTGA ACAAAATTAA AAATCTGGGT CAGCAGCTCT 
     2461 ACATTGAGTC AATGACCAAA TCATCATTCA AGCCAGTGAC GGGGACACGC ACAGAACCGA 
     2521 ATGGTGGTGG TGAGTCAGCG AATGGTGGTG GTGAATGTAA GACCTTTACT TCCTTCCACC 
     2581 AAACACTGAA AAACAATAGT GTGTACACTG AGCCCTGTGA GGATTTGAGG AACGATGAGC 
     2641 ACAGCCCATC CTATCAACAG ATCAACTGTA TCGACAGTGT CATCAGATAC CTGAAGAGCT 
     2701 ACAACATTCC AGCTTTGAAA AGAAAGTGTA TCTCCTGTAC AAATACAACT TCTTCCTCCT 
     2761 CAGAAGAAGA CAAACAGAAC CACAAGGCAG ATGATGTCCA AGCCTTACAA GCTGGTTTGC 
     2821 AAATCCCAGC CATACCTAAA TCAGAAATGC CAACAAATGG ACGGTCCATA GACACAGGAG 
     2881 GAGGAGCTCC ACAGATCCTG TCCACGGCGA TGCTGAGCTT GGGGTCGGGC ATAAGCCAAT 
     2941 GCGGTTACAG CAGCACCATT GTCCATGTCC CACCCCCAGA GACAGCCAGG GATGCTACCC 
     3001 TCTTCTGTGA GCCCTGGACC CTGAACATGC AGCCAGCCCC TTTGACCTCG GAAGAATTTA 
     3061 AACACGTGGG GCTCACAGCG GCTGTTCTGT CAGCGCACAC CCAGAAGGAA GAGCAGAATT 
     3121 ATGTTGATAA ATTCCGAGAA AAGATCCTGT CATCACCCTA CAGCTCCTAT CTTCAGCAAG 
     3181 AAAGCAGGAG CAAAGCTAAA TATTCATATT TTCAAGGAGA TTCTACTTCC AAGCAGACGC 
     3241 GGTCGGCCGG CTGCAGGAAA GGGAAGCACA AGCGGAAGAA GCTGCCGGAG CCGCCAGACA 
     3301 GCAGCAGCTC GAACACCGGC TCTGGTCCCC GCAGGGGAGC GCATCAGAAC GCACAGCCCT 
     3361 GCTGCCCCTC CGCGGCCTCC TCTCCGCACA CCTCGAGCCC GACCTTCCCA CCTGCCGCCA 
     3421 TGGTGCCCAG CCAGGCCCCT TACCTCGTCC CAGCTTTTCC CCTCCCAGCC GCGACCTCAC 
     3481 CCGGAAGAGA ATACGCAGCC CCCGGAACTG CACCGGAAGG CCTGCATGGG CTGCCCTTGT 
     3541 CCGAGGGCTT GCAGCCTTAC CCAGCTTTCC CTTTTCCTTA CTTGGATACT TTTATGACCG 
     3601 TTTTCCTGCC TGACCCCCCT GTCTGTCCTC TGTTGTCGCC ATCGTTTTTG CCATGTCCAT 
     3661 TCCTGGGGGC GACAGCCTCT TCTGCGATAT CACCCTCAAT GTCGTCAGCA ATGAGTCCAA 
     3721 CTCTGGACCC ACCCCCTTCA GTCACCAGCC AAAGGAGAGA GGAGGAAAAG TGGGAGGCAC 
     3781 AAAGCGAGGG GCACCCGTTC ATTACTTCGA GAAGCAGCTC ACCCTTGCAG TTAAACTTAC 
     3841 TTCAGGAAGA GATGCCCAGA CCCTCTGAAT CTCCAGATCA GATGAGAAGG AACACGTGCC 
     3901 CACAAACTGA GTATCAGTGT GTTACAGGCA ACAATGGCAG TGAGAGCAGT CCTGCTACTA 
     3961 CCGGTGCACT GTCCACGGGG TCACCTCCCA GGGAGAATCC ATCCCATCCT ACTGCCAGCG 
     4021 CTCTGTCCAC AGGATCGCCT CCCATGAAGA ATCCATCCCA TCCTACTGCC AGCGCTCTGT 
     4081 CCACAGGATC GCCTCCCATG AAGAATCCAT CCCATCCTAC TGCCAGCACA CTGTCCATGG 
     4141 GATTGCCTCC CAGCAGGACT CCATCCCATC CTACTGCCAC TGTTCTGTCC ACGGGGTCAC 
     4201 CTCCCAGCGA ATCCCCATCC AGAACTGGTT CAGCAGCATC AGGAAGCAGC GACAGCAGTA 
     4261 TATACCTTAC TAGTAGTGTT TATTCTTCTA AAATCTCCCA AAATGGGCAG CAATCTCAGG 
     4321 ACGTACAGAA AAAAGAAACA TTTCCTAATG TCGCCGAAGA GCCCATCTGG AGAATGATAC 
     4381 GGCAGACACC TGAGCGCATT CTCATGACAT ACCAGGTACC TGAGAGGGTT AAAGAAGTTG 
     4441 TACTAAAAGA AGACCTGGAA AAGCTAGAAA GTATGAGGCA GCAGCAGCCC CAGTTTTCTC 
     4501 ATGGGCAAAA GGAGGAGCTG GCTAAGGTGT ATAATTGGAT TCAAAGCCAG ACTGTCACTC 
     4561 AAGAAATCGA CATTCAAGCC TGTGTCACTT GTGAAAATGA AGATTCAGCT GATGGTGCGG 
     4621 CCACATCCTG TGGTCAGGTT CTGGTAGAAG ACAGCTGTAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//