LOCUS       RC240035                8454 bp    DNA     circular  SYN 
DEFINITION  PER3 (myc-DDK-tagged) - Human period circadian clock 3 (PER3), transcript variant 2
ACCESSION   NM_001289861
VERSION     NM_001289861.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="PER3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCGGG GAAGCTCCTG GCCCCGGGAG ACGGGGGGCT AAGGACGAGG 
     1081 CCCTGGGCGA AGAATCGGGG GAGCGGTGGA GCCCCGAGTT CCATCTGCAG AGGAAATTGG 
     1141 CGGACAGCAG CCACAGTGAA CAGCAAGATC GAAACAGAGT TTCTGAAGAA CTTATCATGG 
     1201 TTGTCCAAGA AATGAAAAAA TACTTCCCCT CGGAGAGACG CAATAAACCA AGCACTCTAG 
     1261 ATGCCCTCAA CTATGCTCTC CGCTGTGTCC ACAGCGTTCA AGCAAACAGT GAGTTTTTCC 
     1321 AGATTCTCAG TCAGAATGGA GCACCTCAGG CAGATGTGAG CATGTACAGT CTTGAGGAGC 
     1381 TGGCCACTAT CGCTTCAGAA CACACTTCCA AAAACACAGA TACCTTTGTG GCAGTATTTT 
     1441 CATTTCTGTC TGGAAGGTTA GTGCACATTT CTGAACAGGC TGCTTTGATC CTGAATCGTA 
     1501 AGAAAGATGT CCTGGCGTCT TCTCACTTTG TTGACCTGCT TGCACCTCAA GACATGAGGG 
     1561 TATTCTACGC GCACACTGCC AGAGCTCAGC TTCCTTTCTG GAACAACTGG ACCCAAAGAG 
     1621 CAGCTGCACG GTATGAATGT GCTCCGGTGA AACCTTTTTT CTGCAGGATC CGTGGAGGTG 
     1681 AAGACAGAAA GCAAGAGAAG TGTCACTCCC CATTCCGGAT CATCCCCTAT CTGATTCATG 
     1741 TACATCACCC TGCCCAGCCA GAATTGGAAT CGGAACCTTG CTGTCTCACT GTGGTTGAAA 
     1801 AGATTCACTC TGGTTATGAA GCTCCTCGGA TCCCAGTGAA TAAAAGAATC TTCACCACCA 
     1861 CACACACCCC AGGGTGTGTT TTTCTTGAAG TAGATGAAAA AGCAGTGCCT TTGCTGGGTT 
     1921 ACCTACCTCA GGACCTGATT GGAACATCGA TCCTAAGCTA CCTGCACCCT GAAGATCGTT 
     1981 CTCTGATGGT TGCCATACAC CAAAAAGTTT TGAAGTATGC AGGGCATCCT CCCTTTGAAC 
     2041 ATTCTCCCAT TCGATTTTGT ACTCAAAACG GAGACTACAT CATACTGGAT TCCAGTTGGT 
     2101 CCAGCTTTGT GAATCCCTGG AGCCGGAAGA TTTCTTTCAT CATTGGTCGG CATAAAGTTC 
     2161 GAACGAGCCC ACTAAATGAG GATGTTTTTG CTACCAAAAT TAAAAAGATG AACGATAATG 
     2221 ACAAAGACAT AACAGAATTA CAAGAACAAA TTTACAAACT TCTCTTACAG CCAGTTCACG 
     2281 TGAGCGTGTC CAGCGGCTAC GGGAGCCTGG GGAGCAGCGG GTCGCAGGAG CAGCTTGTCA 
     2341 GCATCGCCTC CTCCAGTGAG GCCAGTGGGC ACCGTGTGGA GGAGACGAAG GCGGAGCAGA 
     2401 TGACCTTGCA GCAGGTCTAT GCCAGTGTGA ACAAAATTAA AAATCTGGGT CAGCAGCTCT 
     2461 ACATTGAGTC AATGACCAAA TCATCATTCA AGCCAGTGAC GGGGACACGC ACAGAACCGA 
     2521 ATGGTGGTGG TGAGTCAGCG AATGGTGGTG GTGAATGTAA GACCTTTACT TCCTTCCACC 
     2581 AAACACTGAA AAACAATAGT GTGTACACTG AGCCCTGTGA GGATTTGAGG AACGATGAGC 
     2641 ACAGCCCATC CTATCAACAG ATCAACTGTA TCGACAGTGT CATCAGATAC CTGAAGAGCT 
     2701 ACAACATTCC AGCTTTGAAA AGAAAGTGTA TCTCCTGTAC AAATACAACT TCTTCCTCCT 
     2761 CAGAAGAAGA CAAACAGAAC CACAAGGCAG ATGATGTCCA AGCCTTACAA GCTGGTTTGC 
     2821 AAATCCCAGC CATACCTAAA TCAGAAATGC CAACAAATGG ACGGTCCATA GACACAGGAG 
     2881 GAGGAGCTCC ACAGATCCTG TCCACGGCGA TGCTGAGCTT GGGGTCGGGC ATAAGCCAAT 
     2941 GCGGTTACAG CAGCACCATT GTCCATGTCC CACCCCCAGA GACAGCCAGG GATGCTACCC 
     3001 TCTTCTGTGA GCCCTGGACC CTGAACATGC AGCCAGCCCC TTTGACCTCG GAAGAATTTA 
     3061 AACACGTGGG GCTCACAGCG GCTGTTCTGT CAGCGCACAC CCAGAAGGAA GAGCAGAATT 
     3121 ATGTTGATAA ATTCCGAGAA AAGATCCTGT CATCACCCTA CAGCTCCTAT CTTCAGCAAG 
     3181 AAAGCAGGAG CAAAGCTAAA TATTCATATT TTCAAGGAGA TTCTACTTCC AAGCAGACGC 
     3241 GGTCGGCCGG CTGCAGGAAA GGGAAGCACA AGCGGAAGAA GCTGCCGGAG CCGCCAGACA 
     3301 GCAGCAGCTC GAACACCGGC TCTGGTCCCC GCAGGGGAGC GCATCAGAAC GCACAGCCCT 
     3361 GCTGCCCCTC CGCGGCCTCC TCTCCGCACA CCTCGAGCCC GACCTTCCCA CCTGCCGCCA 
     3421 TGGTGCCCAG CCAGGCCCCT TACCTCGTCC CAGCTTTTCC CCTCCCAGCC GCGACCTCAC 
     3481 CCGGAAGAGA ATACGCAGCC CCCGGAACTG CACCGGAAGG CCTGCATGGG CTGCCCTTGT 
     3541 CCGAGGGCTT GCAGCCTTAC CCAGCTTTCC CTTTTCCTTA CTTGGATACT TTTATGACCG 
     3601 TTTTCCTGCC TGACCCCCCT GTCTGTCCTC TGTTGTCGCC ATCGTTTTTG CCATGTCCAT 
     3661 TCCTGGGGGC GACAGCCTCT TCTGCGATAT CACCCTCAAT GTCGTCAGCA ATGAGTCCAA 
     3721 CTCTGGACCC ACCCCCTTCA GTCACCAGCC AAAGGAGAGA GGAGGAAAAG TGGGAGGCAC 
     3781 AAAGCGAGGG GCACCCGTTC ATTACTTCGA GAAGCAGCTC ACCCTTGCAG TTAAACTTAC 
     3841 TTCAGGAAGA GATGCCCAGA CCCTCTGAAT CTCCAGATCA GATGAGAAGG AACACGTGCC 
     3901 CACAAACTGA GTATTGTGTT ACAGGCAACA ATGGCAGTGA GAGCAGTCCT GCTACTACCG 
     3961 GTGCACTGTC CACGGGGTCA CCTCCCAGGG AGAATCCATC CCATCCTACT GCCAGCGCTC 
     4021 TGTCCACAGG ATCGCCTCCC ATGAAGAATC CATCCCATCC TACTGCCAGC ACACTGTCCA 
     4081 TGGGATTGCC TCCCAGCAGG ACTCCATCCC ATCCTACTGC CACTGTTCTG TCCACGGGGT 
     4141 CACCTCCCAG CGAATCCCCA TCCAGAACTG GTTCAGCAGC ATCAGGAAGC AGCGACAGCA 
     4201 GTATATACCT TACTAGTAGT GTTTATTCTT CTAAAATCTC CCAAAATGGG CAGCAATCTC 
     4261 AGGACGTACA GAAAAAAGAA ACATTTCCTA ATGTCGCCGA AGAGCCCATC TGGAGAATGA 
     4321 TACGGCAGAC ACCTGAGCGC ATTCTCATGA CATACCAGGT ACCTGAGAGG GTTAAAGAAG 
     4381 TTGTACTAAA AGAAGACCTG GAAAAGCTAG AAAGTATGAG GCAGCAGCAG CCCCAGTTTT 
     4441 CTCATGGGCA AAAGGAGGAG CTGGCTAAGG TGTATAATTG GATTCAAAGC CAGACTGTCA 
     4501 CTCAAGAAAT CGACATTCAA GCCTGTGTCA CTTGTGAAAA TGAAGATTCA GCTGATGGTG 
     4561 CGGCCACATC CTGTGGTCAG GTTCTGGTAG AAGACAGCTG TACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//