LOCUS       RC240031                8433 bp    DNA     circular  SYN 
DEFINITION  PER3 (myc-DDK-tagged) - Human period circadian clock 3 (PER3), transcript variant 3
ACCESSION   NM_001289863
VERSION     NM_001289863.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="PER3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCGGG GAAGCTCCTG GCCCCGGGAG ACGGGGGGCT AAGGACGAGG 
     1081 CCCTGGGCGA AGAATCGGGG GAGCGGTGGA GCCCCGAGTT CCATCTGCAG AGGAAATTGG 
     1141 CGGACAGCAG CCACAGTGAA CAGCAAGATC GAAACAGAGT TTCTGAAGAA CTTATCATGG 
     1201 TTGTCCAAGA AATGAAAAAA TACTTCCCCT CGGAGAGACG CAATAAACCA AGCACTCTAG 
     1261 ATGCCCTCAA CTATGCTCTC CGCTGTGTCC ACAGCGTTCA AGCAAACAGT GAGTTTTTCC 
     1321 AGATTCTCAG TCAGAATGGA GCACCTCAGG CAGATGTGAG CATGTACAGT CTTGAGGAGC 
     1381 TGGCCACTAT CGCTTCAGAA CACACTTCCA AAAACACAGA TACCTTTGTG GCAGTATTTT 
     1441 CATTTCTGTC TGGAAGGTTA GTGCACATTT CTGAACAGGC TGCTTTGATC CTGAATCGTA 
     1501 AGAAAGATGT CCTGGCGTCT TCTCACTTTG TTGACCTGCT TGCACCTCAA GACATGAGGG 
     1561 TATTCTACGC GCACACTGCC AGAGCTCAGC TTCCTTTCTG GAACAACTGG ACCCAAAGAG 
     1621 CAGCTGCACG GTATGAATGT GCTCCGGTGA AACCTTTTTT CTGCAGGATC CGTGGAGGTG 
     1681 AAGACAGAAA GCAAGAGAAG TGTCACTCCC CATTCCGGAT CATCCCCTAT CTGATTCATG 
     1741 TACATCACCC TGCCCAGCCA GAATTGGAAT CGGAACCTTG CTGTCTCACT GTGGTTGAAA 
     1801 AGATTCACTC TGGTTATGAA GCTCCTCGGA TCCCAGTGAA TAAAAGAATC TTCACCACCA 
     1861 CACACACCCC AGGGTGTGTT TTTCTTGAAG TAGATGAAAA AGCAGTGCCT TTGCTGGGTT 
     1921 ACCTACCTCA GGACCTGATT GGAACATCGA TCCTAAGCTA CCTGCACCCT GAAGATCGTT 
     1981 CTCTGATGGT TGCCATACAC CAAAAAGTTT TGAAGTATGC AGGGCATCCT CCCTTTGAAC 
     2041 ATTCTCCCAT TCGATTTTGT ACTCAAAACG GAGACTACAT CATACTGGAT TCCAGTTGGT 
     2101 CCAGCTTTGT GAATCCCTGG AGCCGGAAGA TTTCTTTCAT CATTGGTCGG CATAAAGTTC 
     2161 GAACGAGCCC ACTAAATGAG GATGTTTTTG CTACCAAAAT TAAAAAGATG AACGATAATG 
     2221 ACAAAGACAT AACAGAATTA CAAGAACAAA TTTACAAACT TCTCTTACAG CCAGTTCACG 
     2281 TGAGCGTGTC CAGCGGCTAC GGGAGCCTGG GGAGCAGCGG GTCGCAGGAG CAGCTTGTCA 
     2341 GCATCGCCTC CTCCAGTGAG GCCAGTGGGC ACCGTGTGGA GGAGACGAAG GCGGAGCAGA 
     2401 TGACCTTGCA GCAGGTCTAT GCCAGTGTGA ACAAAATTAA AAATCTGGGT CAGCAGCTCT 
     2461 ACATTGAGTC AATGACCAAA TCATCATTCA AGCCAGTGAC GGGGACACGC ACAGAACCGA 
     2521 ATGGTGGTGG TGAATGTAAG ACCTTTACTT CCTTCCACCA AACACTGAAA AACAATAGTG 
     2581 TGTACACTGA GCCCTGTGAG GATTTGAGGA ACGATGAGCA CAGCCCATCC TATCAACAGA 
     2641 TCAACTGTAT CGACAGTGTC ATCAGATACC TGAAGAGCTA CAACATTCCA GCTTTGAAAA 
     2701 GAAAGTGTAT CTCCTGTACA AATACAACTT CTTCCTCCTC AGAAGAAGAC AAACAGAACC 
     2761 ACAAGGCAGA TGATGTCCAA GCCTTACAAG CTGGTTTGCA AATCCCAGCC ATACCTAAAT 
     2821 CAGAAATGCC AACAAATGGA CGGTCCATAG ACACAGGAGG AGGAGCTCCA CAGATCCTGT 
     2881 CCACGGCGAT GCTGAGCTTG GGGTCGGGCA TAAGCCAATG CGGTTACAGC AGCACCATTG 
     2941 TCCATGTCCC ACCCCCAGAG ACAGCCAGGG ATGCTACCCT CTTCTGTGAG CCCTGGACCC 
     3001 TGAACATGCA GCCAGCCCCT TTGACCTCGG AAGAATTTAA ACACGTGGGG CTCACAGCGG 
     3061 CTGTTCTGTC AGCGCACACC CAGAAGGAAG AGCAGAATTA TGTTGATAAA TTCCGAGAAA 
     3121 AGATCCTGTC ATCACCCTAC AGCTCCTATC TTCAGCAAGA AAGCAGGAGC AAAGCTAAAT 
     3181 ATTCATATTT TCAAGGAGAT TCTACTTCCA AGCAGACGCG GTCGGCCGGC TGCAGGAAAG 
     3241 GGAAGCACAA GCGGAAGAAG CTGCCGGAGC CGCCAGACAG CAGCAGCTCG AACACCGGCT 
     3301 CTGGTCCCCG CAGGGGAGCG CATCAGAACG CACAGCCCTG CTGCCCCTCC GCGGCCTCCT 
     3361 CTCCGCACAC CTCGAGCCCG ACCTTCCCAC CTGCCGCCAT GGTGCCCAGC CAGGCCCCTT 
     3421 ACCTCGTCCC AGCTTTTCCC CTCCCAGCCG CGACCTCACC CGGAAGAGAA TACGCAGCCC 
     3481 CCGGAACTGC ACCGGAAGGC CTGCATGGGC TGCCCTTGTC CGAGGGCTTG CAGCCTTACC 
     3541 CAGCTTTCCC TTTTCCTTAC TTGGATACTT TTATGACCGT TTTCCTGCCT GACCCCCCTG 
     3601 TCTGTCCTCT GTTGTCGCCA TCGTTTTTGC CATGTCCATT CCTGGGGGCG ACAGCCTCTT 
     3661 CTGCGATATC ACCCTCAATG TCGTCAGCAA TGAGTCCAAC TCTGGACCCA CCCCCTTCAG 
     3721 TCACCAGCCA AAGGAGAGAG GAGGAAAAGT GGGAGGCACA AAGCGAGGGG CACCCGTTCA 
     3781 TTACTTCGAG AAGCAGCTCA CCCTTGCAGT TAAACTTACT TCAGGAAGAG ATGCCCAGAC 
     3841 CCTCTGAATC TCCAGATCAG ATGAGAAGGA ACACGTGCCC ACAAACTGAG TATTGTGTTA 
     3901 CAGGCAACAA TGGCAGTGAG AGCAGTCCTG CTACTACCGG TGCACTGTCC ACGGGGTCAC 
     3961 CTCCCAGGGA GAATCCATCC CATCCTACTG CCAGCGCTCT GTCCACAGGA TCGCCTCCCA 
     4021 TGAAGAATCC ATCCCATCCT ACTGCCAGCA CACTGTCCAT GGGATTGCCT CCCAGCAGGA 
     4081 CTCCATCCCA TCCTACTGCC ACTGTTCTGT CCACGGGGTC ACCTCCCAGC GAATCCCCAT 
     4141 CCAGAACTGG TTCAGCAGCA TCAGGAAGCA GCGACAGCAG TATATACCTT ACTAGTAGTG 
     4201 TTTATTCTTC TAAAATCTCC CAAAATGGGC AGCAATCTCA GGACGTACAG AAAAAAGAAA 
     4261 CATTTCCTAA TGTCGCCGAA GAGCCCATCT GGAGAATGAT ACGGCAGACA CCTGAGCGCA 
     4321 TTCTCATGAC ATACCAGGTA CCTGAGAGGG TTAAAGAAGT TGTACTAAAA GAAGACCTGG 
     4381 AAAAGCTAGA AAGTATGAGG CAGCAGCAGC CCCAGTTTTC TCATGGGCAA AAGGAGGAGC 
     4441 TGGCTAAGGT GTATAATTGG ATTCAAAGCC AGACTGTCAC TCAAGAAATC GACATTCAAG 
     4501 CCTGTGTCAC TTGTGAAAAT GAAGATTCAG CTGATGGTGC GGCCACATCC TGTGGTCAGG 
     4561 TTCTGGTAGA AGACAGCTGT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//