LOCUS       RC240030                8424 bp    DNA     circular  SYN 
DEFINITION  PLCB2 (myc-DDK-tagged) - Human phospholipase C, beta 2 (PLCB2), transcript variant 2
ACCESSION   NM_001284297
VERSION     NM_001284297.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4581..4672 
                     /label="Myc-DDK"  
     misc_feature    4702..5287 
                     /label="PolyA signal"  
     misc_feature    4759..4778 
                     /label="Restriction Enzyme"  
     misc_feature    5563..6235 
                     /label="ColE1"  
     misc_feature    6665..7459 
                     /label="Kan/Neo"  
     misc_feature    7494..7796 
                     /label="SV40 ori"  
     misc_feature    7882..8359 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4563..4569 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4563 
                     /label="PLCB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCTGCTC AACCCTGTCC TGCTGCCCCC CAAGGTGAAG GCCTATCTGA 
     1081 GCCAAGGGGA GCGCTTCATC AAATGGGATG ATGAAACTAC AGTTGCCTCT CCAGTTATCC 
     1141 TCCGTGTGGA TCCTAAGGGC TACTACTTAT ACTGGACGTA TCAAAGTAAG GAGATGGAGT 
     1201 TTCTGGATAT CACCAGCATC CGGGATACTC GCTTTGGGAA GTTTGCCAAG ATGCCCAAGA 
     1261 GCCAGAAGCT CCGGGACGTC TTCAACATGG ACTTTCCTGA TAACAGTTTC CTGCTGAAGA 
     1321 CACTCACGGT GGTGTCCGGC CCGGACATGG TGGACCTCAC CTTCCACAAC TTCGTCTCCT 
     1381 ACAAGGAGAA CGTGGGCAAG GCCTGGGCTG AGGACGTACT GGCCCTAGTC AAACATCCGC 
     1441 TGACGGCCAA CGCCTCCCGC AGCACCTTCC TGGACAAGAT CCTTGTGAAG CTCAAGATGC 
     1501 AGCTCAACTC TGAAGGGAAG ATTCCGGTGA AGAACTTTTT CCAGATGTTT CCTGCTGACC 
     1561 GCAAGCGGGT GGAAGCTGCT CTCAGTGCCT GCCACCTCCC CAAAGGCAAA AATGACGCCA 
     1621 TCAATCCTGA GGACTTCCCA GAACCTGTCT ACAAGAGTTT CCTCATGAGC CTCTGTCCTC 
     1681 GGCCAGAAAT AGATGAGATC TTCACTTCTT ACCATGCTAA GGCCAAACCC TACATGACGA 
     1741 AGGAGCACCT GACCAAATTC ATCAACCAGA AACAGCGGGA CTCCCGGCTT AACTCCCTGC 
     1801 TGTTCCCGCC AGCACGGCCT GACCAGGTGC AGGGCCTCAT CGACAAGTAT GAGCCCAGTG 
     1861 GCATCAATGC ACAGAGGGGC CAGCTGTCAC CTGAAGGCAT GGTCTGGTTT CTCTGTGGGC 
     1921 CAGAGAACAG CGTGCTGGCC CAGGACAAGC TGCTGCTCCA CCACGACATG ACGCAGCCAC 
     1981 TCAATCATTA CTTCATCAAC TCGTCCCACA ACACCTACCT GACAGCCGGC CAGTTCTCAG 
     2041 GCCTCTCCTC GGCTGAGATG TACCGCCAGG TGCTGCTCTC TGGCTGCCGT TGCGTGGAGC 
     2101 TAGACTGCTG GAAGGGGAAA CCCCCTGACG AGGAGCCCAT TATCACCCAT GGCTTCACCA 
     2161 TGACCACAGA CATCTTCTTC AAAGAAGCAA TTGAGGCTAT TGCAGAAAGC GCCTTTAAGA 
     2221 CCTCCCCCTA TCCCATCATC CTGTCGTTTG AGAACCATGT GGACTCACCC CGCCAGCAGG 
     2281 CTAAGATGGC TGAGTATTGC CGGACGATCT TTGGGGATAT GCTGCTCACA GAGCCCCTGG 
     2341 AAAAGTTCCC ACTAAAACCA GGTGTCCCCC TGCCCAGCCC TGAGGATCTC AGGGGCAAGA 
     2401 TCCTCATCAA GAACAAGAAG AACCAGTTTT CTGGCCCCAC CTCCTCCAGT AAGGATACTG 
     2461 GTGGGGAGGC TGAGGGCAGC AGCCCACCCA GTGCCCCTGC AGTGTGGGCT GGCGAGGAAG 
     2521 GGACTGAGCT GGAGGAGGAG GAGGTGGAAG AGGAAGAGGA GGAGGAGTCA GGAAACCTGG 
     2581 ATGAAGAAGA GATTAAGAAG ATGCAGTCGG ATGAGGGCAC AGCGGGCCTG GAAGTGACGG 
     2641 CTTATGAGGA GATGTCCAGC CTAGTCAATT ACATCCAGCC CACCAAGTTC GTCTCCTTTG 
     2701 AGTTCTCTGC CCAAAAGAAC CGAAGTTATG TCATCTCGTC CTTCACAGAG CTCAAGGCAT 
     2761 ATGACCTGCT CTCCAAGGCC TCGGTGCAGT TTGTGGACTA CAACAAGCGC CAGATGAGCC 
     2821 GCATTTACCC CAAGGGAACC CGCATGGACT CCTCCAACTA CATGCCCCAG ATGTTCTGGA 
     2881 ATGCTGGATG CCAGATGGTT GCCCTCAACT TCCAGACGAT GGACTTGCCC ATGCAGCAGA 
     2941 ACATGGCAGT ATTTGAGTTC AACGGGCAGA GCGGCTACCT CCTCAAGCAT GAGTTCATGC 
     3001 GCCGGCCGGA CAAGCAGTTC AACCCCTTCT CAGTGGACCG CATCGACGTG GTGGTGGCCA 
     3061 CCACCCTTTC CATTACGGTG ATCTCTGGGC AGTTCCTGTC AGAACGCAGC GTGCGCACCT 
     3121 ATGTAGAAGT GGAGCTGTTT GGCCTTCCTG GGGACCCCAA GAGGCGCTAT CGAACTAAGC 
     3181 TGTCACCCAG TACTAACTCC ATCAATCCTG TCTGGAAGGA GGAGCCCTTT GTCTTTGAGA 
     3241 AGATCTTGAT GCCTGAGCTG GCCTCCCTCA GAGTGGCTGT GATGGAGGAA GGCAACAAGT 
     3301 TTCTTGGACA CCGCATCATC CCCATCAATG CCCTAAATTC TGGGTACCAC CACCTGTGCC 
     3361 TGCACAGTGA GAGCAACATG CCCCTCACCA TGCCTGCGCT CTTCATCTTC CTGGAGATGA 
     3421 AGGACTACAT ACCTGGTGCT TGGGCAGATC TCACTGTGGC CCTCGCCAAC CCCATTAAGT 
     3481 TCTTCAGTGC CCATGACACG AAGTCTGTGA AGCTCAAGGA GGCCATGGGA GGTCTGCCTG 
     3541 AGAAGCCCTT CCCACTGGCG AGTCCAGTTG CCAGCCAGGT CAATGGGGCG TTGGCCCCAA 
     3601 CGAGCAATGG GTCACCAGCA GCCAGGGCCG GGGCCAGGGA AGAGGCTATG AAAGAAGCTG 
     3661 CGGAGCCGCG GACCGCCAGC CTGGAGGAGC TCCGGGAGCT AAAGGGCGTG GTGAAGCTGC 
     3721 AGCGGCGGCA CGAGAAGGAG CTGCGAGAGT TGGAGCGGCG CGGAGCGCGG CGCTGGGAGG 
     3781 AGCTGCTGCA GCGGGGCGCG GCGCAGCTGG CGGAGCTCGG GCCACCGGGC GTGGGGGGCG 
     3841 TCGGGGCCTG CAAGCTCGGT CCCGGCAAGG GCTCTCGCAA GAAGAGGAGC CTGCCCCGCG 
     3901 AGGAGAGCGC CGGAGCCGCG CCGGGCGAGG GCCCTGAGGG CGTGGACGGG CGCGTGCGGG 
     3961 AGCTGAAAGA CAGGCTGGAG CTGGAGCTGC TGCGGCAGGG CGAGGAGCAG TACGAGTGCG 
     4021 TTCTGAAGCG CAAGGAGCAG CACGTGGCCG AGCAAATCTC CAAAATGATG GAGCTGGCCA 
     4081 GAGAGAAACA GGCGGCAGAG CTGAAGGCCC TGAAGGAGAC GTCGGAGAAC GACACCAAAG 
     4141 AGATGAAGAA AAAGCTGGAG ACAAAGAGAC TGGAGCGGAT CCAGGGCATG ACCAAAGTCA 
     4201 CCACAGACAA GATGGCCCAG GAGAGGTTGA AGAGAGAGAT TAACAACTCC CACATCCAGG 
     4261 AAGTAGTGCA GGTGATCAAG CAGATGACGG AGAACTTGGA GAGGCACCAG GAGAAGCTGG 
     4321 AGGAGAAGCA GGCGGCTTGC CTGGAACAGA TACGGGAGAT GGAAAAGCAG TTCCAGAAGG 
     4381 AGGCGCTGGC AGAGTACGAG GCCAGGATGA AGGGTCTGGA GGCAGAGGTG AAGGAGTCGG 
     4441 TGAGGGCCTG CCTCAGGACC TGCTTTCCCT CCGAGGCCAA GGACAAGCCT GAGAGGGCCT 
     4501 GCGAGTGCCC CCCAGAGCTG TGTGAGCAGG ACCCACTCAT AGCAAAGGCA GATGCCCAGG 
     4561 AGAGCCGCCT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4621 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5401 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5461 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5521 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5581 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5641 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5701 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5761 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5821 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5881 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5941 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6001 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6241 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6301 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6361 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6421 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6481 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6541 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6601 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6661 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6721 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6781 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6841 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6901 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6961 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7021 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7081 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7141 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7201 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7261 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7321 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7381 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7441 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7501 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7561 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7621 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7861 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7921 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7981 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8041 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8101 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8161 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8221 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8281 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8341 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8401 TAACAAAAAT TTAACGCGAA TTTT
//