LOCUS       RC240028                8424 bp    DNA     circular  SYN 
DEFINITION  DHX8 (myc-DDK-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant 2
ACCESSION   NM_001302623
VERSION     NM_001302623.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4581..4672 
                     /label="Myc-DDK"  
     misc_feature    4702..5287 
                     /label="PolyA signal"  
     misc_feature    4759..4778 
                     /label="Restriction Enzyme"  
     misc_feature    5563..6235 
                     /label="ColE1"  
     misc_feature    6665..7459 
                     /label="Kan/Neo"  
     misc_feature    7494..7796 
                     /label="SV40 ori"  
     misc_feature    7882..8359 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4563..4569 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4563 
                     /label="DHX8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGGCT GTAGCCATGG CGGGAGCCTT AATCGGGTCG GAGCCAGGCC 
     1081 CCGCGGAAGA ACTTGCCAAA CTCGAGTACC TGTCTTTGGT GTCAAAGGTT TGCACTGAGC 
     1141 TGGACAATCA CTTGGGGATC AACGACAAGG ACCTTGCTGA ATTTGTGATC AGTCTTGCTG 
     1201 AGAAAAATAC CACCTTTGAT ACTTTTAAGG CTTCTCTCGT CAAAAATGGT GCAGAATTTA 
     1261 CGGATTCTCT TATTAGTAAC TTGCTGCGTC TCATACAAAC CATGCGGCCT CCAGCGAAGC 
     1321 CTTCCACTAG CAAAGATCCA GTTGTTAAAC CTAAAACAGA AAAAGAAAAG CTGAAGGAAC 
     1381 TCTTCCCAGT CCTTTGCCAA CCGGACAACC CTTCTGTTCG GACCATGTTG GATGAAGATG 
     1441 ATGTGAAAGT TGCTGTGGAT GTCCTGAAAG AACTGGAAGC TTTAATGCCC AGCGCAGCAG 
     1501 GCCAGGAGAA GCAAAGAGAT GCTGAACACC GGGACAGGAC AAAGAAGAAG AAGCGGAGTC 
     1561 GAAGCCGAGA TCGAAACCGA GATCGAGACA GAGATAGGGA ACGAAACCGA GATAGAGACC 
     1621 ACAAGCGGAG ACACCGATCC CGCTCTCGAT CACGTTCCAG GACCCGGGAG AGGAATAAAG 
     1681 TGAAGTCTAG ATATCGGTCC AGGAGCAGGA GTCAGAGTCC CCCCAAAGAC CGGAAGGACC 
     1741 GGGACAAATA TGGAGAGCGG AATCTGGATA GATGGCGGGA TAAGCATGTG GACCGCCCTC 
     1801 CTCCAGAAGA GCCCACCATT GGTGACATTT ATAATGGCAA AGTTACCAGC ATCATGCAGT 
     1861 TTGGTTGCTT TGTGCAGCTG GAAGGACTAA GGAAGCGGTG GGAAGGCCTG GTGCACATCT 
     1921 CTGAGCTCCG GCGGGAGGGT CGTGTGGCCA ATGTAGCTGA TGTCGTGAGC AAAGGCCAGA 
     1981 GGGTCAAAGT CAAAGTGCTG TCCTTCACTG GGACCAAGAC CAGCCTGAGC ATGAAGGATG 
     2041 TGGATCAAGA GACTGGAGAA GATCTAAACC CAAATAGACG GCGAAATCTT GTCGGGGAGA 
     2101 CCAATGAGGA GACCTCAATG CGGAATCCTG ATAGACCCAC TCACTTGTCC CTTGTCAGTG 
     2161 CTCCTGAAGT AGAGGACGAC TCACTGGAAC GCAAGCGCCT CACCCGAATC TCTGACCCAG 
     2221 AGAAGTGGGA GATCAAACAG ATGATTGCTG CCAATGTCCT TTCCAAAGAA GAATTTCCAG 
     2281 ACTTTGATGA AGAGACTGGC ATTCTCCCTA AGGTGGATGA TGAAGAAGAT GAGGACCTTG 
     2341 AGATTGAATT GGTTGAGGAA GAGCCTCCAT TCCTGAGAGG GCACACTAAG CAAAGCATGG 
     2401 ACATGAGCCC CATTAAAATT GTCAAGAACC CAGACGGCTC CCTCTCCCAA GCAGCAATGA 
     2461 TGCAGAGTGC CTTGGCCAAA GAAAGGCGGG AACTCAAACA GGCCCAGCGG GAAGCTGAGA 
     2521 TGGATTCTAT TCCCATGGGA CTCAACAAAC ACTGGGTTGA CCCTCTGCCT GATGCGGAAG 
     2581 GCAGACAGAT TGCTGCCAAC ATGAGGGGTA TTGGGATGAT GCCCAATGAT ATTCCTGAGT 
     2641 GGAAGAAGCA TGCCTTTGGG GGCAACAAAG CCTCTTACGG AAAAAAGACC CAGATGTCAA 
     2701 TCCTTGAGCA GAGGGAGAGC CTGCCCATCT ACAAACTGAA GGAGCAATTG GTCCAGGCCG 
     2761 TCCATGACAA TCAGATCCTG ATTGTCATTG GTGAGACAGG ATCTGGAAAG ACAACACAGA 
     2821 TCACCCAGTA CCTGGCGGAG GCAGGCTACA CTTCCAGGGG CAAGATTGGG TGTACCCAGC 
     2881 CCAGAAGAGT GGCAGCTATG TCGGTGGCCA AAAGAGTGTC AGAGGAGTTT GGTTGTTGCT 
     2941 TAGGCCAAGA GGTGGGCTAC ACCATTCGAT TTGAGGACTG CACTAGCCCT GAAACAGTCA 
     3001 TCAAGTACAT GACAGATGGG ATGTTGCTTA GAGAGTGCTT GATTGACCCT GACCTCACTC 
     3061 AGTACGCGAT CATCATGTTG GACGAGGCAC ATGAGAGGAC AATTCACACT GATGTGCTCT 
     3121 TTGGATTGTT GAAAAAGACA GTTCAGAAAC GGCAGGACAT GAAGCTGATT GTCACCTCAG 
     3181 CCACCTTGGA TGCAGTGAAG TTTTCTCAAT ACTTCTATGA AGCTCCCATT TTCACCATCC 
     3241 CAGGTCGAAC ATATCCAGTG GAAATACTGT ACACAAAGGA ACCTGAGACA GATTATCTGG 
     3301 ATGCCAGCCT GATTACTGTT ATGCAGATTC ATTTAACAGA ACCACCAGGT GATATCCTGG 
     3361 TCTTCCTGAC TGGTCAGGAA GAAATTGATA CTGCTTGTGA GATCCTGTAT GAAAGAATGA 
     3421 AATCCCTGGG ACCTGATGTT CCAGAGTTAA TTATCCTCCC AGTGTACTCT GCTCTTCCCA 
     3481 GTGAGATGCA GACCCGAATC TTTGACCCAG CTCCACCAGG CAGCAGAAAG GTTGTGATTG 
     3541 CCACCAATAT CGCAGAGACA TCGCTGACTA TTGATGGTAT CTACTATGTG GTGGACCCAG 
     3601 GATTCGTGAA ACAGAAAGTT TACAATTCCA AGACAGGGAT TGACCAGCTC GTGGTGACGC 
     3661 CTATTTCTCA GGCTCAGGCA AAGCAACGAG CTGGCAGAGC TGGGAGAACA GGCCCAGGGA 
     3721 AGTGTTACAG GTTGTACACA GAACGTGCCT ACCGAGATGA AATGCTGACC ACCAACGTGC 
     3781 CGGAAATCCA GAGAACCAAC TTAGCAAGCA CAGTGCTGTC ACTCAAGGCC ATGGGTATCA 
     3841 ATGATCTGCT GTCCTTTGAT TTCATGGATG CCCCACCTAT GGAAACTTTG ATCACAGCCA 
     3901 TGGAGCAGCT GTACACACTG GGGGCCCTGG ATGACGAGGG CCTGCTCACT CGCTTGGGCC 
     3961 GCCGGATGGC AGAGTTCCCT CTGGAGCCAA TGCTATGCAA AATGCTCATC ATGTCTGTGC 
     4021 ATCTGGGCTG CAGTGAGGAA ATGCTGACCA TTGTATCCAT GCTGTCTGTG CAGAACGTCT 
     4081 TCTATAGGCC CAAGGATAAA CAAGCCCTTG CAGATCAGAA GAAGGCCAAA TTCCACCAGA 
     4141 CTGAAGGGGA CCACCTCACC CTGCTAGCTG TGTACAACTC CTGGAAGAAC AACAAGTTCT 
     4201 CCAACCCATG GTGCTATGAG AACTTTATCC AGGCTCGTTC CCTGCGCCGG GCCCAGGACA 
     4261 TTCGCAAGCA GATGTTAGGC ATAATGGACA GACACAAGCT GGATGTTGTT TCCTGTGGCA 
     4321 AGTCCACAGT CCGAGTGCAG AAGGCCATCT GCAGTGGGTT CTTCCGTAAT GCTGCCAAGA 
     4381 AAGACCCGCA GGAGGGTTAC CGGACACTGA TCGACCAGCA GGTGGTCTAT ATCCATCCTT 
     4441 CCAGTGCCCT CTTCAACAGA CAGCCAGAAT GCCCAAAGCA CTTCCTCCCT GTGGTTGCTG 
     4501 TAGCTGTCTC ATTGGAGCAG TGCCTCTCCA AGTTTGAGGA TTTGAACAAA GAACTGGGAC 
     4561 TGGTGACTTG TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4621 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5401 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5461 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5521 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5581 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5641 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5701 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5761 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5821 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5881 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5941 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6001 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6241 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6301 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6361 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6421 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6481 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6541 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6601 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6661 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6721 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6781 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6841 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6901 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6961 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7021 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7081 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7141 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7201 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7261 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7321 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7381 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7441 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7501 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7561 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7621 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7861 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7921 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7981 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8041 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8101 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8161 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8221 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8281 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8341 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8401 TAACAAAAAT TTAACGCGAA TTTT
//