LOCUS       RC240019                8388 bp    DNA     circular  SYN 
DEFINITION  ITGAX (myc-DDK-tagged) - Human integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), transcript variant 1
ACCESSION   NM_001286375
VERSION     NM_001286375.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4545..4636 
                     /label="Myc-DDK"  
     misc_feature    4666..5251 
                     /label="PolyA signal"  
     misc_feature    4723..4742 
                     /label="Restriction Enzyme"  
     misc_feature    5527..6199 
                     /label="ColE1"  
     misc_feature    6629..7423 
                     /label="Kan/Neo"  
     misc_feature    7458..7760 
                     /label="SV40 ori"  
     misc_feature    7846..8323 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4527..4533 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4527 
                     /label="ITGAX"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCAGGACC AGGGCAGCAC TCCTCCTGTT CACAGCCTTA GCAACTTCTC 
     1081 TAGGTTTCAA CTTGGACACA GAGGAGCTGA CAGCCTTCCG TGTGGACAGC GCTGGGTTTG 
     1141 GAGACAGCGT GGTCCAGTAT GCCAACTCCT GGGTGGTGGT TGGAGCCCCC CAAAAGATAA 
     1201 CAGCTGCCAA CCAAACGGGT GGCCTCTACC AGTGTGGCTA CAGCACTGGT GCCTGTGAGC 
     1261 CCATCGGCCT GCAGGTGCCC CCGGAGGCCG TGAACATGTC CCTGGGCCTG TCCCTGGCGT 
     1321 CTACCACCAG CCCTTCCCAG CTGCTGGCCT GCGGCCCCAC CGTGCACCAC GAGTGCGGGA 
     1381 GGAACATGTA CCTCACCGGA CTCTGCTTCC TCCTGGGCCC CACCCAGCTC ACCCAGAGGC 
     1441 TCCCGGTGTC CAGGCAGGAG TGCCCAAGAC AGGAGCAGGA CATTGTGTTC CTGATCGATG 
     1501 GCTCAGGCAG CATCTCCTCC CGCAACTTTG CCACGATGAT GAACTTCGTG AGAGCTGTGA 
     1561 TAAGCCAGTT CCAGAGACCC AGCACCCAGT TTTCCCTGAT GCAGTTCTCC AACAAATTCC 
     1621 AAACACACTT CACTTTCGAG GAATTCAGGC GCAGCTCAAA CCCCCTCAGC CTGTTGGCTT 
     1681 CTGTTCACCA GCTGCAAGGG TTTACATACA CGGCCACCGC CATCCAAAAT GTCGTGCACC 
     1741 GATTGTTCCA TGCCTCATAT GGGGCCCGTA GGGATGCCGC CAAAATTCTC ATTGTCATCA 
     1801 CTGATGGGAA GAAAGAAGGC GACAGCCTGG ATTATAAGGA TGTCATCCCC ATGGCTGATG 
     1861 CAGCAGGCAT CATCCGCTAT GCAATTGGGG TTGGATTAGC TTTTCAAAAC AGAAATTCTT 
     1921 GGAAAGAATT AAATGACATT GCATCGAAGC CCTCCCAGGA ACACATATTT AAAGTGGAGG 
     1981 ACTTTGATGC TCTGAAAGAT ATTCAAAACC AACTGAAGGA GAAGATCTTT GCCATTGAGG 
     2041 GTACGGAGAC CACAAGCAGT AGCTCCTTCG AATTGGAGAT GGCACAGGAG GGCTTCAGCG 
     2101 CTGTGTTCAC ACCTGATGGC CCCGTTCTGG GGGCTGTGGG GAGCTTCACC TGGTCTGGAG 
     2161 GTGCCTTCCT GTACCCCCCA AATATGAGCC CTACCTTCAT CAACATGTCT CAGGAGAATG 
     2221 TGGACATGAG GGACTCTTAC CTGGGTTACT CCACCGAGCT GGCCCTCTGG AAAGGGGTGC 
     2281 AGAGCCTGGT CCTGGGGGCC CCCCGCTACC AGCACACCGG GAAGGCTGTC ATCTTCACCC 
     2341 AGGTGTCCAG GCAATGGAGG ATGAAGGCCG AAGTCACGGG GACTCAGATC GGCTCCTACT 
     2401 TCGGGGCCTC CCTCTGCTCC GTGGACGTAG ACAGCGACGG CAGCACCGAC CTGGTCCTCA 
     2461 TCGGGGCCCC CCATTACTAC GAGCAGACCC GAGGGGGCCA GGTGTCTGTG TGTCCCTTGC 
     2521 CCAGGGGGTG GAGAAGGTGG TGGTGTGATG CTGTTCTCTA CGGGGAGCAG GGCCACCCCT 
     2581 GGGGTCGCTT TGGGGCGGCT CTGACAGTGC TGGGGGATGT GAATGGGGAC AAGCTGACAG 
     2641 ACGTGGTCAT CGGGGCCCCA GGAGAGGAGG AGAACCGGGG TGCTGTCTAC CTGTTTCACG 
     2701 GAGTCTTGGG ACCCAGCATC AGCCCCTCCC ACAGCCAGCG GATCGCGGGC TCCCAGCTCT 
     2761 CCTCCAGGCT GCAGTATTTT GGGCAGGCAC TGAGCGGGGG TCAAGACCTC ACCCAGGATG 
     2821 GACTGGTGGA CCTGGCTGTG GGGGCCCGGG GCCAGGTGCT CCTGCTCAGG ACCAGACCTG 
     2881 TGCTCTGGGT GGGGGTGAGC ATGCAGTTCA TACCTGCCGA GATCCCCAGG TCTGCGTTTG 
     2941 AGTGTCGGGA GCAGGTGGTC TCTGAGCAGA CCCTGGTACA GTCCAACATC TGCCTTTACA 
     3001 TTGACAAACG TTCTAAGAAC CTGCTTGGGA GCCGTGACCT CCAAAGCTCT GTGACCTTGG 
     3061 ACCTGGCCCT CGACCCTGGC CGCCTGAGTC CCCGTGCCAC CTTCCAGGAA ACAAAGAACC 
     3121 GGAGTCTGAG CCGAGTCCGA GTCCTCGGGC TGAAGGCACA CTGTGAAAAC TTCAACCTGC 
     3181 TGCTCCCGAG CTGCGTGGAG GACTCTGTGA CCCCCATTAC CTTGCGTCTG AACTTCACGC 
     3241 TGGTGGGCAA GCCCCTCCTT GCCTTCAGAA ACCTGCGGCC TATGCTGGCC GCCGATGCTC 
     3301 AGAGATACTT CACGGCCTCC CTACCCTTTG AGAAGAACTG TGGAGCCGAC CATATCTGCC 
     3361 AGGACAATCT CGGCATCTCC TTCAGCTTCC CAGGCTTGAA GTCCCTGCTG GTGGGGAGTA 
     3421 ACCTGGAGCT GAACGCAGAA GTGATGGTGT GGAATGACGG GGAAGACTCC TACGGAACCA 
     3481 CCATCACCTT CTCCCACCCC GCAGGACTGT CCTACCGCTA CGTGGCAGAG GGCCAGAAAC 
     3541 AAGGGCAGCT GCGTTCCCTG CACCTGACAT GTGACAGCGC CCCAGTTGGG AGCCAGGGCA 
     3601 CCTGGAGCAC CAGCTGCAGA ATCAACCACC TCATCTTCCG TGGCGGCGCC CAGATCACCT 
     3661 TCTTGGCTAC CTTTGACGTC TCCCCCAAGG CTGTCCTGGG AGACCGGCTG CTTCTGACAG 
     3721 CCAATGTGAG CAGTGAGAAC AACACTCCCA GGACCAGCAA GACCACCTTC CAGCTGGAGC 
     3781 TCCCGGTGAA GTATGCTGTC TACACTGTGG TTAGCAGCCA CGAACAATTC ACCAAATACC 
     3841 TCAACTTCTC AGAGTCTGAG GAGAAGGAAA GCCATGTGGC CATGCACAGA TACCAGGTCA 
     3901 ATAACCTGGG ACAGAGGGAC CTGCCTGTCA GCATCAACTT CTGGGTGCCT GTGGAGCTGA 
     3961 ACCAGGAGGC TGTGTGGATG GATGTGGAGG TCTCCCACCC CCAGAACCCA TCCCTTCGGT 
     4021 GCTCCTCAGA GAAAATCGCA CCCCCAGCAT CTGACTTCCT GGCGCACATT CAGAAGAATC 
     4081 CCGTGCTGGA CTGCTCCATT GCTGGCTGCC TGCGGTTCCG CTGTGACGTC CCCTCCTTCA 
     4141 GCGTCCAGGA GGAGCTGGAT TTCACCCTGA AGGGCAACCT CAGCTTTGGC TGGGTCCGCC 
     4201 AGATATTGCA GAAGAAGGTG TCGGTCGTGA GTGTGGCTGA AATTACGTTC GACACATCCG 
     4261 TGTACTCCCA GCTTCCAGGA CAGGAGGCAT TTATGAGAGC TCAGACGACA ACGGTGCTGG 
     4321 AGAAGTACAA GGTCCACAAC CCCACCCCCC TCATCGTAGG CAGCTCCATT GGGGGTCTGT 
     4381 TGCTGCTGGC ACTCATCACA GCGGTACTGT ACAAAGTTGG CTTCTTCAAG CGTCAGTACA 
     4441 AGGAAATGAT GGAGGAGGCA AATGGACAAA TTGCCCCAGA AAACGGGACA CAGACCCCCA 
     4501 GCCCGCCCAC TCCCCATTAC CCTCAGGACA ATGTCACGCG TACGCGGCCG CTCGAGCAGA 
     4561 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4621 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4681 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4741 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4801 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4861 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4921 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4981 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5041 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5101 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5161 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5221 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5281 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5341 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5401 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5461 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5521 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5581 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5641 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5701 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5761 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5821 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5881 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5941 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6001 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6061 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6121 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6181 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6241 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6301 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6361 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6421 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6481 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6541 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6601 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6661 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6721 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6781 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6841 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6901 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6961 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7021 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7081 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7141 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7201 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7261 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7321 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7381 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7441 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7501 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7561 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7621 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7801 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7861 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7921 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7981 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8041 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8101 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8161 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8221 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8281 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8341 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//