LOCUS       RC240016                8382 bp    DNA     circular  SYN 
DEFINITION  STAG3 (myc-DDK-tagged) - Human stromal antigen 3 (STAG3), transcript variant 4
ACCESSION   NM_001282718
VERSION     NM_001282718.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4539..4630 
                     /label="Myc-DDK"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    4717..4736 
                     /label="Restriction Enzyme"  
     misc_feature    5521..6193 
                     /label="ColE1"  
     misc_feature    6623..7417 
                     /label="Kan/Neo"  
     misc_feature    7452..7754 
                     /label="SV40 ori"  
     misc_feature    7840..8317 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="STAG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTCCCCG TTGCAAAGAG CTGTGGGAGA TACCAAGAGG GCCTTGTCTG 
     1081 CATCTTCTAG TTCCTCTGCC AGTCTACCCT TTGATGACAG GGACTCAAAC CATACCTCAG 
     1141 AGGGGAATGG CGACTCTTTG TTAGCTGATG AAGACACTGA CTTTGAAGAC AGCTTGAATC 
     1201 GCAATGTGAA GAAGAGAGCA GCAAAACGAC CACCGAAAAC AACACCGGTG GCAAAACATC 
     1261 CAAAGAAAGG GTCCCGAGTG GTACATCGTC ATAGCCGGAA ACAGTCAGAG CCACCAGCCA 
     1321 ATGATCTTTT CAATGCTGTG AAAGCCGCCA AAAGTGACAT GCAGGACTCG GGGGACTACC 
     1381 CTCTCATAGC TCCAGGTCCA TCCTGGAAGA AGTTCCAGGG CAGCTTCTGT GAATTTGTGA 
     1441 GGACATTGGT CTGTCAGTGC CAGTACAGCC TCCTCTATGA TGGCTTCCCT ATGGACGACC 
     1501 TCATCTCCCT GCTCACTGGC CTCTCAGACT CACAAGTCCG CGCCTTCCGT CACACTAGCA 
     1561 CCCTGGCTGC TATGAAACTG ATGACCTCCC TGGTAAAAGT TGCCCTCCAA CTGAGTGTGC 
     1621 ACCAAGATAA CAATCAGCGT CAGTATGAGG CTGAAAGAAA CAAGGGGCCA GGGCAGAGGG 
     1681 CACCTGAGCG GCTGGAGAGC CTGTTGGAGA AACGCAAAGA GCTCCAAGAG CATCAAGAGG 
     1741 AGATTGAGGG GATGATGAAT GCCCTCTTCA GGGGTGTCTT TGTTCATCGG TACAGGGATG 
     1801 TCCTTCCTGA GATCCGTGCT ATCTGCATTG AGGAAATTGG GTGTTGGATG CAAAGCTACA 
     1861 GCACGTCTTT CCTCACCGAC AGCTATTTAA AATATATTGG TTGGACTCTG CATGATAAGC 
     1921 ACCGAGAAGT CCGCCTGAAG TGTGTGAAGG CCCTGAAAGG GCTGTACGGT AACCGGGACC 
     1981 TGACCACACG CCTGGAGCTC TTCACCAGCC GCTTCAAGGA CCGGATGGTT TCCATGGTCA 
     2041 TGGACAGAGA GTATGATGTG GCAGTGGAGG CTGTCAGATT ACTGATACTT ATCCTTAAGA 
     2101 ACATGGAAGG GGTGCTGACG GACGCGGATT GTGAGAGCGT CTACCCAGTT GTGTATGCCT 
     2161 CTCATCGAGG CCTGGCCTCT GCCGCAGGCG AATTTCTGTA CTGGAAACTC TTCTACCCTG 
     2221 AGTGCGAGAT AAGAATGATG GGTGGAAGAG AGCAACGCCA GAGCCCAGGC GCCCAGAGGA 
     2281 CTTTCTTCCA GCTTCTGCTG TCCTTCTTTG TGGAGAGCGA GCTCCATGAC CACGCTGCTT 
     2341 ACTTAGTAGA CAGTCTGTGG GACTGTGCAG GGGCTCGGCT GAAGGACTGG GAGGGTCTGA 
     2401 CAAGCCTGCT GCTGGAGAAG GACCAGAACC TGGGTGATGT GCAGGAGAGC ACACTGATAG 
     2461 AAATCCTTGT GTCCAGTGCC CGGCAAGCTT CAGAGGGGCA CCCGCCTGTG GGCCGGGTCA 
     2521 CTGGGAGGAA GGGCTTAACC TCTAAGGAGC GCAAGACCCA AGCCGATGAC AGGGTGAAGT 
     2581 TGACTGAGCA CCTCATCCCC CTGCTGCCCC AGCTCCTGGC CAAGTTCTCA GCTGATGCAG 
     2641 AGAAGGTCAC TCCCCTGCTC CAGCTTCTCA GCTGCTTTGA CCTCCACATC TACTGCACTG 
     2701 GGCGCTTGGA GAAGCACCTG GAGCTGTTCC TGCAGCAACT CCAGGAGGTG GTGGTGAAGC 
     2761 ATGCAGAGCC AGCGGTGCTT GAGGCTGGGG CGCATGCCCT CTACCTGCTC TGTAATCCCG 
     2821 AATTCACTTT CTTCAGCCGG GCGGACTTTG CCCGCAGCCA GCTAGTAGAT TTGCTGACTG 
     2881 ACCGCTTCCA GCAGGAGCTT GAAGAGCTGT TACAGTCGTC CTTCCTAGAT GAGGATGAGG 
     2941 TATATAATCT GGCAGCCACT CTGAAACGCC TCTCTGCCTT CTACAACACT CATGACCTGA 
     3001 CTCGCTGGGA GCTCTATGAG CCATGTTGCC AACTCCTGCA GAAGGCTGTG GACACAGGAG 
     3061 AGGTTCCTCA CCAGGTTATC CTGCCAGCCT TGACTCTTGT CTATTTTTCC ATTCTCTGGA 
     3121 CACTAACCCA CATTTCTAAA TCAGATGCTT CCCAGAAGCA GCTGTCGAGT TTGAGGGACA 
     3181 GAATGGTGGC CTTCTGTGAA CTCTGCCAGA GTTGCCTCTC AGATGTGGAT ACTGAGATCC 
     3241 AGGAGCAGGC TTTTGTCTTA TTAAGTGATC TACTTCTCAT CTTTAGCCCT CAGATGATTG 
     3301 TTGGGGGCCG TGATTTCCTT AGGCCACTTG TCTTTTTTCC TGAAGCTACT CTCCAGTCTG 
     3361 AGCTAGCCAG CTTCCTCATG GACCACGTCT TCATCCAGCC GGGAGACCTG GGCAGTGGTG 
     3421 ATTCCCAGGA GGATCATTTA CAGATAGAGC GGCTACACCA GCGGCGCCGC CTCCTAGCCG 
     3481 GGTTCTGCAA GCTGTTGCTT TATGGGGTGC TGGAGATGGA TGCAGCCTCA GATGTTTTCA 
     3541 AACACTACAA CAAGTTCTAC AATGACTATG GTGACATTAT CAAGGAAACA TTAACTAGAG 
     3601 CAAGGCAGAT TGACCGAAGT CATTGTTCCC GAATCCTGCT GCTGAGCCTC AAGCAGCTGT 
     3661 ACACAGAACT GCTGCAGGAG CATGGGCCCC AGGGCCTGAA TGAGCTTCCT GCCTTCATCG 
     3721 AGATGAGGGA CCTGGCCCGG AGGTTTGCCT TGAGTTTTGG ACCCCAGCAG CTGCAGAACC 
     3781 GTGACCTCGT GGTCATGCTA CACAAGGAAG GCATCCAGTT CTCCTTGTCT GAGCTTCCTC 
     3841 CAGCTGGCTC CTCCAATCAG CCTCCAAATC TGGCATTCCT GGAGCTCCTT TCAGAGTTTT 
     3901 CCCCCCGACT CTTCCATCAG GACAAGCAGC TTTTACTGTC CTATCTAGAA AAGTGCCTGC 
     3961 AGCATGTCTC CCAGGCACCT GGCCATCCCT GGGGCCCAGT CACCACCTAC TGCCACTCCC 
     4021 TCAGCCCTGT GGAGAACACA GCAGAGACCA GCCCTCAGGT CCTCCCCAGC TCCAAGAGGA 
     4081 GGCGCGTTGA AGGGCCTGCC AAGCCTAACA GAGAGGACGT CTCCTCGTCC CAGGAAGAAA 
     4141 GTCTGCAGCT GAACAGCATC CCGCCCACGC CCACCCTCAC CTCCACAGCT GTGAAGAGCA 
     4201 GGCAGCCCCT GTGGGGGTTG AAAGAGATGG AGGAAGAAGA TGGCTCAGAG TTGGATTTTG 
     4261 CCCAGGGTCA GCCCGTCGCA GGCACCGAGA GGTCAAGGTT CTTGGGTCCA CAATATTTCC 
     4321 AGACTCCACA CAACCCTTCA GGTCCTGGCC TGGGCAACCA GCTGATGCGA CTCAGCCTTA 
     4381 TGGAAGAGGA CGAGGAAGAA GAGTTAGAAA TCCAGGATGA GTCAAATGAA GAACGGCAGG 
     4441 ATACAGACAT GCAAGCAAGT AGCTACTCTT CCACCAGTGA GCGCGGGCTG GACCTCTTAG 
     4501 ATTCTACAGA GCTGGATATT GAGGATTTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4561 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5341 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5401 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5461 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5521 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5581 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5641 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5701 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5761 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5821 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5881 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5941 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6001 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6061 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6121 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6181 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6241 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6301 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6361 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6421 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6481 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6541 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6601 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6661 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6721 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6781 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6841 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6901 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6961 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7021 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7081 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7141 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7201 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7261 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7321 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7381 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7441 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7501 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7561 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7621 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7801 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7861 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7921 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7981 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8041 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8101 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8161 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8221 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8281 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8341 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//