LOCUS       RC240015                8382 bp    DNA     circular  SYN 
DEFINITION  POLR2B (myc-DDK-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 3
ACCESSION   NM_001303269
VERSION     NM_001303269.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4539..4630 
                     /label="Myc-DDK"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    4717..4736 
                     /label="Restriction Enzyme"  
     misc_feature    5521..6193 
                     /label="ColE1"  
     misc_feature    6623..7417 
                     /label="Kan/Neo"  
     misc_feature    7452..7754 
                     /label="SV40 ori"  
     misc_feature    7840..8317 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="POLR2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAATATGAT GAGGATGATG ATGAAATCAC CCCGGATTTG TGGCAAGAAG 
     1081 CATGCTGGAT TGTAATCAGT TCCTATTTTG ACGAGAAAGG CTTGGTTAGA CAACAGCTGG 
     1141 ATTCTTTTGA TGAGTTTATT CAGATGTCTG TTCAAAGAAT TGTGGAAGAC GCTCCTCCTA 
     1201 TAGACCTACA GGCTGAAGCT CAGCATGCTA GTGGAGAAGT TGAAGAACCG CCACGATATT 
     1261 TGCTGAAGTT TGAACAAATT TATCTTTCCA AGCCTACCCA TTGGGAAAGA GATGGTGCTC 
     1321 CTTCACCAAT GATGCCCAAT GAAGCTAGAT TAAGGAATCT CACGTATTCT GCTCCGCTTT 
     1381 ATGTTGATAT AACAAAAACA GTCATTAAAG AAGGTGAAGA ACAACTTCAG ACTCAGCATC 
     1441 AGAAAACTTT TATAGGAAAA ATTCCAATTA TGTTGCGGTC AACTTACTGC CTTTTGAATG 
     1501 GCTTGACAGA TCGTGATCTT TGTGAGTTAA ATGAATGCCC TTTGGATCCT GGTGGCTATT 
     1561 TCATTATTAA TGGATCAGAA AAGGTTCTGA TTGCCCAAGA GAAAATGGCA ACAAACACAG 
     1621 TTTATGTGTT TGCCAAAAAG GATTCTAAAT ATGCCTACAC AGGAGAGTGT AGATCATGTC 
     1681 TTGAGAATTC TTCCCGACCC ACCAGTACTA TATGGGTTAG CATGCTGGCA AGAGGAGGAC 
     1741 AGGGTGCCAA GAAGAGTGCT ATTGGTCAGC GCATTGTGGC AACTCTACCA TATATCAAGC 
     1801 AAGAAGTTCC CATCATTATT GTGTTCAGAG CATTAGGTTT TGTGTCCGAC AGAGATATTT 
     1861 TAGAACATAT TATTTATGAT TTTGAAGATC CAGAGATGAT GGAAATGGTT AAACCTTCTC 
     1921 TCGATGAAGC TTTTGTCATC CAAGAACAGA ATGTTGCACT AAATTTCATT GGTTCAAGAG 
     1981 GAGCAAAGCC TGGTGTTACT AAAGAGAAAA GAATTAAATA TGCAAAGGAA GTTTTACAAA 
     2041 AAGAAATGCT CCCTCATGTT GGTGTCAGTG ATTTTTGTGA GACCAAAAAA GCCTATTTCT 
     2101 TGGGATACAT GGTTCATAGG TTACTTCTGG CAGCTTTGGG TAGAAGAGAA CTAGATGACA 
     2161 GAGATCACTA TGGAAACAAG AGATTGGATC TTGCTGGGCC GCTGCTTGCA TTCTTATTTA 
     2221 GAGGTATGTT TAAGAATTTG CTTAAAGAAG TGCGGATCTA TGCACAGAAA TTTATTGATC 
     2281 GAGGAAAGGA TTTTAACTTG GAGTTGGCAA TTAAAACACG GATCATATCT GATGGCCTAA 
     2341 AATACTCTTT AGCTACTGGA AACTGGGGTG ATCAAAAGAA AGCTCATCAA GCCAGAGCTG 
     2401 GAGTATCTCA GGTGTTAAAC CGCCTGACTT TTGCGTCTAC TCTTTCTCAC CTGCGTCGTT 
     2461 TAAATTCTCC TATTGGTAGA GACGGCAAGC TAGCAAAACC AAGACAGTTG CATAATACGT 
     2521 TGTGGGGAAT GGTGTGTCCT GCCGAGACCC CAGAGGGCCA TGCTGTAGGA CTTGTGAAGA 
     2581 ATTTAGCCTT GATGGCGTAT ATTTCAGTTG GATCTCAACC ATCTCCAATT CTGGAATTTT 
     2641 TAGAAGAATG GAGTATGGAA AATTTAGAAG AAATTTCTCC TGCAGCTATT GCTGATGCAA 
     2701 CCAAGATTTT TGTTAATGGC TGCTGGGTTG GAATACATAA AGATCCCGAA CAACTTATGA 
     2761 ACACCCTAAG GAAATTGAGA CGTCAGATGG ACATCATTGT GTCTGAAGTT TCTATGATCA 
     2821 GAGATATTCG AGAGAGGGAG ATTCGGATCT ATACGGATGC AGGCCGTATT TGTAGACCAC 
     2881 TTCTGATTGT GGAAAAACAA AAGCTACTTT TGAAGAAGAG GCATATTGAC CAATTGAAAG 
     2941 AGAGAGAATA TAACAACTAT AGTTGGCAGG ATCTTGTGGC CAGTGGGGTA GTGGAGTATA 
     3001 TTGATACCCT GGAAGAAGAA ACAGTGATGC TTGCAATGAC TCCAGATGAT TTACAGGAGA 
     3061 AAGAAGTAGC TTATTGTTCC ACATATACAC ACTGTGAGAT TCATCCCTCA ATGATCCTTG 
     3121 GTGTCTGTGC ATCTATTATT CCCTTTCCTG ATCATAACCA GTCCCCTAGA AACACATACC 
     3181 AGTCTGCTAT GGGTAAGCAG GCTATGGGAG TTTACATCAC CAACTTCCAT GTTCGCATGG 
     3241 ACACATTGGC CCATGTTCTC TATTATCCTC AAAAGCCACT TGTGACTACA CGGTCTATGG 
     3301 AATATCTACG ATTTAGAGAG CTGCCAGCAG GCATCAACTC AATTGTGGCC ATTGCATCAT 
     3361 ACACTGGATA TAATCAGGAA GACTCTGTTA TCATGAATCG TTCAGCTGTA GACCGCGGCT 
     3421 TCTTCAGGTC TGTTTTCTAT CGCTCATACA AAGAACAGGA GTCTAAAAAA GGATTTGATC 
     3481 AAGAAGAAGT TTTTGAGAAG CCTACACGTG AAACATGCCA GGGCATGAGG CATGCCATTT 
     3541 ACGACAAGCT GGATGATGAT GGTTTGATAG CTCCAGGGGT TCGTGTATCA GGAGATGATG 
     3601 TTATTATAGG CAAAACAGTC ACCTTGCCTG AAAATGAAGA TGAATTGGAG AGCACCAATA 
     3661 GACGCTATAC CAAGAGAGAC TGTAGCACTT TTCTCAGAAC TAGCGAGACG GGCATTGTGG 
     3721 ATCAGGTTAT GGTAACTCTC AATCAGGAAG GATATAAATT TTGTAAAATA AGGGTACGCT 
     3781 CTGTTAGGAT TCCACAGATT GGAGACAAAT TTGCTAGTCG ACATGGTCAA AAGGGTACTT 
     3841 GTGGTATTCA GTATAGACAA GAGGATATGC CTTTCACCTG TGAAGGTATC ACCCCTGATA 
     3901 TCATCATCAA TCCCCATGCC ATCCCCTCTC GTATGACTAT TGGTCACTTA ATTGAATGCC 
     3961 TTCAAGGGAA GGTATCGGCT AACAAGGGTG AAATTGGTGA TGCCACTCCA TTTAATGATG 
     4021 CTGTTAACGT GCAGAAGATT TCTAATCTTT TATCTGATTA TGGCTATCAT CTCAGAGGAA 
     4081 ATGAGGTCCT GTACAATGGG TTCACTGGTC GAAAAATCAC ATCACAAATA TTTATTGGCC 
     4141 CCACTTATTA CCAGCGTTTG AAGCATATGG TGGATGATAA GATTCACTCT CGTGCTAGGG 
     4201 GACCTATTCA GATCCTCAAT AGACAGCCCA TGGAGGGTAG ATCTCGTGAT GGTGGCCTGC 
     4261 GTTTTGGAGA AATGGAACGA GATTGTCAGA TTGCCCATGG AGCAGCCCAG TTTTTAAGGG 
     4321 AAAGATTGTT TGAGGCATCA GATCCATATC AGGTTCATGT TTGCAATCTT TGTGGAATAA 
     4381 TGGCGATTGC CAACACCAGG ACCCATACAT ATGAATGCAG GGGCTGCCGC AATAAAACCC 
     4441 AGATTTCTTT GGTGCGAATG CCTTACGCAT GCAAACTATT GTTTCAGGAA CTTATGTCTA 
     4501 TGAGTATTGC ACCGCGAATG ATGAGTGTTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4561 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5341 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5401 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5461 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5521 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5581 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5641 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5701 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5761 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5821 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5881 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5941 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6001 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6061 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6121 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6181 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6241 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6301 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6361 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6421 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6481 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6541 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6601 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6661 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6721 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6781 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6841 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6901 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6961 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7021 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7081 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7141 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7201 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7261 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7321 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7381 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7441 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7501 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7561 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7621 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7801 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7861 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7921 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7981 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8041 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8101 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8161 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8221 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8281 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8341 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//