LOCUS       RC240008                8346 bp    DNA     circular  SYN 
DEFINITION  ACLY (myc-DDK-tagged) - Human ATP citrate lyase (ACLY), transcript variant 3
ACCESSION   NM_001303274
VERSION     NM_001303274.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4503..4594 
                     /label="Myc-DDK"  
     misc_feature    4624..5209 
                     /label="PolyA signal"  
     misc_feature    4681..4700 
                     /label="Restriction Enzyme"  
     misc_feature    5485..6157 
                     /label="ColE1"  
     misc_feature    6587..7381 
                     /label="Kan/Neo"  
     misc_feature    7416..7718 
                     /label="SV40 ori"  
     misc_feature    7804..8281 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4485..4491 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4485 
                     /label="ACLY"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCGGGG AAAAGTCCGG CTGGGCCGGG ACAAAAGCCG GATCCCGGGA 
     1081 AGCTACCGGC TGCTGGGGTG CTCCGGATTT TGCGGGGTTC GTCGGGCCTG TGGAAGAAGC 
     1141 GCCGCGCACG GACTTCGGCA GAGACAGGTA GAGCAGGTCT CTCTGCAGCC ATGTCGGCCA 
     1201 AGGCAATTTC AGAGCAGACG GGCAAAGAAC TCCTTTACAA GTTCATCTGT ACCACCTCAG 
     1261 CCATCCAGAA TCGGTTCAAG TATGCTCGGG TCACTCCTGA CACAGACTGG GCCCGCTTGC 
     1321 TGCAGGACCA CCCCTGGCTG CTCAGCCAGA ACTTGGTAGT CAAGCCAGAC CAGCTGATCA 
     1381 AACGTCGTGG AAAACTTGGT CTCGTTGGGG TCAACCTCAC TCTGGATGGG GTCAAGTCCT 
     1441 GGCTGAAGCC ACGGCTGGGA CAGGAAGCCA CAGTTGGCAA GGCCACAGGC TTCCTCAAGA 
     1501 ACTTTCTGAT CGAGCCCTTC GTCCCCCACA GTCAGGCTGA GGAGTTCTAT GTCTGCATCT 
     1561 ATGCCACCCG AGAAGGGGAC TACGTCCTGT TCCACCACGA GGGGGGTGTG GACGTGGGTG 
     1621 ATGTGGACGC CAAGGCCCAG AAGCTGCTTG TTGGCGTGGA TGAGAAACTG AATCCTGAGG 
     1681 ACATCAAAAA ACACCTGTTG GTCCACGCCC CTGAAGACAA GAAAGAAATT CTGGCCAGTT 
     1741 TTATCTCCGG CCTCTTCAAT TTCTACGAGG ACTTGTACTT CACCTACCTC GAGATCAATC 
     1801 CCCTTGTAGT GACCAAAGAT GGAGTCTATG TCCTTGACTT GGCGGCCAAG GTGGACGCCA 
     1861 CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATCGA GTTCCCTCCC CCCTTCGGGC 
     1921 GGGAGGCATA TCCAGAGGAA GCCTACATTG CAGACCTCGA TGCCAAAAGT GGGGCAAGCC 
     1981 TGAAGCTGAC CTTGCTGAAC CCCAAAGGGA GGATCTGGAC CATGGTGGCC GGGGGTGGCG 
     2041 CCTCTGTCGT GTACAGCGAT ACCATCTGTG ATCTAGGGGG TGTCAACGAG CTGGCAAACT 
     2101 ATGGGGAGTA CTCAGGCGCC CCCAGCGAGC AGCAGACCTA TGACTATGCC AAGACTATCC 
     2161 TCTCCCTCAT GACCCGAGAG AAGCACCCAG ATGGCAAGAT CCTCATCATT GGAGGCAGCA 
     2221 TCGCAAACTT CACCAACGTG GCTGCCACGT TCAAGGGCAT CGTGAGAGCA ATTCGAGATT 
     2281 ACCAGGGCCC CCTGAAGGAG CACGAAGTCA CAATCTTTGT CCGAAGAGGT GGCCCCAACT 
     2341 ATCAGGAGGG CTTACGGGTG ATGGGAGAAG TCGGGAAGAC CACTGGGATC CCCATCCATG 
     2401 TCTTTGGCAC AGAGACTCAC ATGACGGCCA TTGTGGGCAT GGCCCTGGGC CACCGGCCCA 
     2461 TCCCCAACCA GCCACCCACA GCGGCCCACA CTGCAAACTT CCTCCTCAAC GCCAGCGGGA 
     2521 GCACATCGAC GCCAGCCCCC AGCAGGACAG CATCTTTTTC TGAGTCCAGG GCCGATGAGG 
     2581 TGGCGCCTGC AAAGAAGGCC AAGCCTGCCA TGCCACAAGA TTCAGTCCCA AGTCCAAGAT 
     2641 CCCTGCAAGG AAAGAGCACC ACCCTCTTCA GCCGCCACAC CAAGGCCATT GTGTGGGGCA 
     2701 TGCAGACCCG GGCCGTGCAA GGCATGCTGG ACTTTGACTA TGTCTGCTCC CGAGACGAGC 
     2761 CCTCAGTGGC TGCCATGGTC TACCCTTTCA CTGGGGACCA CAAGCAGAAG TTTTACTGGG 
     2821 GGCACAAAGA GATCCTGATC CCTGTCTTCA AGAACATGGC TGATGCCATG AGGAAGCATC 
     2881 CGGAGGTAGA TGTGCTCATC AACTTTGCCT CTCTCCGCTC TGCCTATGAC AGCACCATGG 
     2941 AGACCATGAA CTATGCCCAG ATCCGGACCA TCGCCATCAT AGCTGAAGGC ATCCCTGAGG 
     3001 CCCTCACGAG AAAGCTGATC AAGAAGGCGG ACCAGAAGGG AGTGACCATC ATCGGACCTG 
     3061 CCACTGTTGG AGGCATCAAG CCTGGGTGCT TTAAGATTGG CAACACAGGT GGGATGCTGG 
     3121 ACAACATCCT GGCCTCCAAA CTGTACCGCC CAGGCAGCGT GGCCTATGTC TCACGTTCCG 
     3181 GAGGCATGTC CAACGAGCTC AACAATATCA TCTCTCGGAC CACGGATGGC GTCTATGAGG 
     3241 GCGTGGCCAT TGGTGGGGAC AGGTACCCGG GCTCCACATT CATGGATCAT GTGTTACGCT 
     3301 ATCAGGACAC TCCAGGAGTC AAAATGATTG TGGTTCTTGG AGAGATTGGG GGCACTGAGG 
     3361 AATATAAGAT TTGCCGGGGC ATCAAGGAGG GCCGCCTCAC TAAGCCCATC GTCTGCTGGT 
     3421 GCATCGGGAC GTGTGCCACC ATGTTCTCCT CTGAGGTCCA GTTTGGCCAT GCTGGAGCTT 
     3481 GTGCCAACCA GGCTTCTGAA ACTGCAGTAG CCAAGAACCA GGCTTTGAAG GAAGCAGGAG 
     3541 TGTTTGTGCC CCGGAGCTTT GATGAGCTTG GAGAGATCAT CCAGTCTGTA TACGAAGATC 
     3601 TCGTGGCCAA TGGAGTCATT GTACCTGCCC AGGAGGTGCC GCCCCCAACC GTGCCCATGG 
     3661 ACTACTCCTG GGCCAGGGAG CTTGGTTTGA TCCGCAAACC TGCCTCGTTC ATGACCAGCA 
     3721 TCTGCGATGA GCGAGGACAG GAGCTCATCT ACGCGGGCAT GCCCATCACT GAGGTCTTCA 
     3781 AGGAAGAGAT GGGCATTGGC GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA AGGTTGCCTA 
     3841 AGTACTCTTG CCAGTTCATT GAGATGTGTC TGATGGTGAC AGCTGATCAC GGGCCAGCCG 
     3901 TCTCTGGAGC CCACAACACC ATCATTTGTG CGCGAGCTGG GAAAGACCTG GTCTCCAGCC 
     3961 TCACCTCGGG GCTGCTCACC ATCGGGGATC GGTTTGGGGG TGCCTTGGAT GCAGCAGCCA 
     4021 AGATGTTCAG TAAAGCCTTT GACAGTGGCA TTATCCCCAT GGAGTTTGTG AACAAGATGA 
     4081 AGAAGGAAGG GAAGCTGATC ATGGGCATTG GTCACCGAGT GAAGTCGATA AACAACCCAG 
     4141 ACATGCGAGT GCAGATCCTC AAAGATTACG TCAGGCAGCA CTTCCCTGCC ACTCCTCTGC 
     4201 TCGATTATGC ACTGGAAGTA GAGAAGATTA CCACCTCGAA GAAGCCAAAT CTTATCCTGA 
     4261 ATGTAGATGG TCTCATCGGA GTCGCATTTG TAGACATGCT TAGAAACTGT GGGTCCTTTA 
     4321 CTCGGGAGGA AGCTGATGAA TATATTGACA TTGGAGCCCT CAATGGCATC TTTGTGCTGG 
     4381 GAAGGAGTAT GGGGTTCATT GGACACTATC TTGATCAGAA GAGGCTGAAG CAGGGGCTGT 
     4441 ATCGTCATCC GTGGGATGAT ATTTCATATG TTCTTCCGGA ACACATGAGC ATGACGCGTA 
     4501 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4561 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5281 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5341 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5401 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5461 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5521 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5581 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5641 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5701 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5761 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5821 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5881 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5941 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6001 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6061 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6121 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6181 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6241 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6301 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6361 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6421 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6481 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6541 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6601 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6661 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6721 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6781 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6841 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6901 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6961 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7021 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7081 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7141 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7201 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7261 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7321 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7381 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7801 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7861 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7921 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7981 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8041 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8101 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8161 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8221 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8281 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8341 AATTTT
//