LOCUS       RC239999                8286 bp    DNA     circular  SYN 
DEFINITION  CCAR1 (myc-DDK-tagged) - Human cell division cycle and apoptosis regulator 1 (CCAR1), transcript variant 2
ACCESSION   NM_001282959
VERSION     NM_001282959.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="CCAR1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAATTT GGAGGACAGA AGAATCCGCC ATGGGCTACT CAGTTTACAG 
     1081 CCACTGCAGT ATCACAGCCA GCTGCACTGG GTGTTCAACA GCCATCACTC CTTGGAGCAT 
     1141 CTCCTACCAT TTATACACAG CAAACTGCAT TGGCAGCAGC AGGCCTTACC ACACAAACTC 
     1201 CAGCAAACTA TCAGTTAACA CAAACTGCTG CATTGCAGCA ACAAGCCGCA GCTGCAGCAG 
     1261 CTGCATTACA ACAGTTACAG CAACCCCAGC AAACCCTCTT AACACAGCCA GCTGTTGCAC 
     1321 TGCCTACAAG CCTTAGCCTG TCTACTCCTC AGCCAACAGC ACAAATAACT GTATCATATC 
     1381 CAACACCAAG GTCCAGTCAA CAGCAAACCC AGCCTCAGAA GCAGCGTGTT TTCACAGGGG 
     1441 TGGTTACAAA ACTACATGAT ACGTTTGGAT TTGTGGATGA AGATGTATTC TTTCAGCTTA 
     1501 GTGCTGTCAA AGGGAAAACC CCCCAAGTAG GTGACAGAGT ATTGGTTGAA GCTACTTATA 
     1561 ATCCTAATAT GCCTTTTAAA TGGAATGCAC AGAGAATTCA AACACTACCA AATCAGAATC 
     1621 AGTCGCAAAC CCAGCCATTA CTGAAGACTC CTCCTGCTGT ACTTCAGCCA ATTGCACCAC 
     1681 AGACAACATT TGGTGTTCAG ACTCAGCCCC AGCCCCAGTC ACTGCTGCAG GCACAGATTT 
     1741 CAGCAGCTTC TATTACACCA CTATTGCAGA CTCAACCACA GCCCTTATTA CAGCAGCCTC 
     1801 AGCAAAAAGC TGGTTTATTG CAGCCTCCTG TTCGTATAGT TTCACAGCCA CAACCGGCAC 
     1861 GACGATTAGA TCCCCCATCC CGATTTTCAG GAAGAAATGA CAGAGGGGAT CAAGTGCCTA 
     1921 ACAGAAAAGA TGATCGAAGT CGTGAGAGAG AGAGAGAAAG ACGTAGATCG AGAGAAAGAT 
     1981 CACCTCAGAG GAAACGTTCC CGGGAAAGAT CTCCACGAAG AGAGCGAGAG CGATCACCTC 
     2041 GGAGAGTTCG ACGTGTTGTT CCACGTTACA CAGTTCAGTT TTCAAAGTTT TCTTTAGATT 
     2101 GTCCCAGTTG TGACATGATG GAACTAAGGC GCCGTTATCA AAATTTGTAT ATACCTAGTG 
     2161 ACTTTTTTGA TGCTCAATTT ACATGGGTGG ATGCTTTCCC TTTGTCAAGA CCATTTCAGC 
     2221 TGGGAAATTA CTGCAATTTT TATGTAATGC ACAGAGAAGT AGAGTCCTTA GAAAAAAATA 
     2281 TGGCCATTCT TGATCCACCA GATGCTGACC ACTTATACAG TGCAAAGGTA ATGCTGATGG 
     2341 CTAGCCCTAG TATGGAAGAT TTATATCATA AGTCATGTGC TCTTGCTGAG GACCCACAAG 
     2401 AACTTCGAGA TGGATTCCAA CATCCTGCTA GACTTGTTAA GTTTTTAGTG GGCATGAAAG 
     2461 GCAAGGATGA AGCTATGGCC ATTGGAGGCC ACTGGTCTCC TTCGTTGGAT GGACCAGACC 
     2521 CAGAAAAAGA TCCCTCTGTG TTGATTAAGA CTGCTATTCG TTGTTGTAAG GCTCTGACAG 
     2581 GCATTGATCT AAGTGTGTGC ACACAATGGT ACCGTTTTGC AGAGATTCGC TACCATCGCC 
     2641 CTGAGGAGAC CCACAAGGGG CGTACAGTTC CAGCTCATGT GGAGACAGTG GTTTTATTTT 
     2701 TCCCGGATGT TTGGCATTGC CTTCCCACCC GCTCAGAGTG GGAAACCCTC TCCCGAGGAT 
     2761 ACAAGCAGCA GCTGGTCGAG AAGCTTCAGG GTGAACGCAA GGAGGCTGAT GGAGAACAGG 
     2821 ATGAAGAAGA GAAGGATGAT GGTGAAGCTA AAGAAATTTC TACACCTACC CATTGGTCTA 
     2881 AACTTGATCC AAAGACAATG AAGGTAAATG ACCTCCGAAA AGAATTAGAA AGTCGAGCTC 
     2941 TTAGTTCCAA AGGATTAAAA TCCCAGTTAA TAGCCCGATT GACAAAACAG CTTAAAGTAG 
     3001 AGGAACAAAA AGAAGAACAG AAGGAGTTAG AGAAATCTGA AAAAGAAGAG GATGAGGATG 
     3061 ATGATAGGAA ATCTGAAGAC GATAAAGAGG AAGAAGAAAG GAAACGTCAA GAGGAAATAG 
     3121 AACGCCAGCG TCGAGAAAGA AGATATATTT TGCCTGATGA ACCGGCCATC ATTGTACATC 
     3181 CAAATTGGGC TGCAAAAAGT GGCAAGTTTG ATTGTAGCAT CATGTCTTTG AGTGTCCTAT 
     3241 TGGACTACAG ATTAGAGGAT AATAAAGAAC ATTCATTTGA GGTTTCATTG TTTGCGGAAC 
     3301 TTTTCAACGA AATGCTTCAA AGAGATTTTG GTGTCCGTAT ATACAAATCA TTACTGTCTC 
     3361 TTCCTGAGAA AGAGGACAAA AAAGAAAAGG ATAAAAAAAG CAAAAAAGAT GAGAGAAAAG 
     3421 ATAAAAAAGA AGAAAGAGAT GATGAAACTG ATGAACCAAA ACCCAAACGG AGAAAATCAG 
     3481 GCGATGATAA AGATAAAAAA GAAGATAGAG ATGAAAGGAA GAAAGAAGAT AAAAGAAAAG 
     3541 ATGATTCTAA AGATGATGAT GAAACTGAAG AAGATAACAA TCAAGATGAA TATGACCCTA 
     3601 TGGAAGCAGA AGAAGCTGAG GATGAAGAAG ATGATAGGGA TGAGGAAGAA ATGACCAAAC 
     3661 GAGATGACAA AAGAGATATC AACAGATACT GCAAGGAGAG GCCCTCTAAA GATAAGGAAA 
     3721 AAGAAAAGAC TCAAATGATC ACAATTAACA GAGATCTGTT AATGGCTTTT GTTTATTTTG 
     3781 ATCAAAGTCA TTGTGGTTAC CTTCTTGAAA AGGATTTGGA AGAAATACTT TATACTCTTG 
     3841 GACTACATCT TTCTCGGGCT CAGGTAAAGA AGCTTCTTAA TAAAGTAGTG CTCCGTGAAT 
     3901 CTTGCTTTTA CCGGAAATTA ACAGACACCT CAAAAGATGA AGAGAACCAT GAAGAGTCTG 
     3961 AGTCATTGCA GGAAGATATG CTAGGAAACA GATTATTACT TCCAACACCA ACAGTAAAGC 
     4021 AGGAATCAAA GGATGTGGAA GAAAATGTTG GCCTCATTGT GTACAATGGT GCAATGGTAG 
     4081 ATGTAGGAAG CCTCTTGCAA AAATTGGAAA AGAGCGAAAA AGTAAGAGCT GAGGTAGAAC 
     4141 AGAAGCTGCA GTTACTAGAA GAAAAAACAG ATGAAGATGA AAAAACCATA TTAAATTTGG 
     4201 AGAATTCCAA CAAAAGCCTC TCTGGTGAAC TCAGAGAAGT TAAAAAGGAC CTTAGTCAGT 
     4261 TACAAGAAAA CTTAAAGATT TCGGAAAACA TGAATTTACA ATTTGAAAAC CAAATGAATA 
     4321 AGACAATCAG AAACTTATCT ACGGTAATGG ATGAAATCCA CACTGTTCTC AAGAAGGATA 
     4381 ATGTAAAGAA TGAAGACAAA GATCAAAAAT CCAAGGAGAA TGGTGCCAGT GTAACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//