LOCUS       RC239994                8274 bp    DNA     circular  SYN 
DEFINITION  CIITA (myc-DDK-tagged) - Human class II, major histocompatibility complex, transactivator (CIITA), transcript variant 1
ACCESSION   NM_001286402
VERSION     NM_001286402.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4431..4522 
                     /label="Myc-DDK"  
     misc_feature    4552..5137 
                     /label="PolyA signal"  
     misc_feature    4609..4628 
                     /label="Restriction Enzyme"  
     misc_feature    5413..6085 
                     /label="ColE1"  
     misc_feature    6515..7309 
                     /label="Kan/Neo"  
     misc_feature    7344..7646 
                     /label="SV40 ori"  
     misc_feature    7732..8209 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4413..4419 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4413 
                     /label="CIITA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGTTGCCTG GCTCCACGCC CTGCTGGGTC CTACCTGTCA GAGCCCCAAG 
     1081 GCAGCTCACA GTGTGCCACC ATGGAGTTGG GGCCCCTAGA AGGTGGCTAC CTGGAGCTTC 
     1141 TTAACAGCGA TGCTGACCCC CTGTGCCTCT ACCACTTCTA TGACCAGATG GACCTGGCTG 
     1201 GAGAAGAAGA GATTGAGCTC TACTCAGAAC CCGACACAGA CACCATCAAC TGCGACCAGT 
     1261 TCAGCAGGCT GTTGTGTGAC ATGGAAGGTG ATGAAGAGAC CAGGGAGGCT TATGCCAATA 
     1321 TCGCGGAACT GGACCAGTAT GTCTTCCAGG ACTCCCAGCT GGAGGGCCTG AGCAAGGACA 
     1381 TTTTCATAGA GCACATAGGA CCAGATGAAG TGATCGGTGA GAGTATGGAG ATGCCAGCAG 
     1441 AAGTTGGGCA GAAAAGTCAG AAAAGACCCT TCCCAGAGGA GCTTCCGGCA GACCTGAAGC 
     1501 ACTGGAAGCC AGCTGAGCCC CCCACTGTGG TGACTGGCAG TCTCCTAGTG GGACCAGTGA 
     1561 GCGACTGCTC CACCCTGCCC TGCCTGCCAC TGCCTGCGCT GTTCAACCAG GAGCCAGCCT 
     1621 CCGGCCAGAT GCGCCTGGAG AAAACCGACC AGATTCCCAT GCCTTTCTCC AGTTCCTCGT 
     1681 TGAGCTGCCT GAATCTCCCT GAGGGACCCA TCCAGTTTGT CCCCACCATC TCCACTCTGC 
     1741 CCCATGGGCT CTGGCAAATC TCTGAGGCTG GAACAGGGGT CTCCAGTATA TTCATCTACC 
     1801 ATGGTGAGGT GCCCCAGGCC AGCCAAGTAC CCCCTCCCAG TGGATTCACT GTCCACGGCC 
     1861 TCCCAACATC TCCAGACCGG CCAGGCTCCA CCAGCCCCTT CGCTCCATCA GCCACTGACC 
     1921 TGCCCAGCAT GCCTGAACCT GCCCTGACCT CCCGAGCAAA CATGACAGAG CACAAGACGT 
     1981 CCCCCACCCA ATGCCCGGCA GCTGGAGAGG TCTCCAACAA GCTTCCAAAA TGGCCTGAGC 
     2041 CGGTGGAGCA GTTCTACCGC TCACTGCAGG ACACGTATGG TGCCGAGCCC GCAGGCCCGG 
     2101 ATGGCATCCT AGTGGAGGTG GATCTGGTGC AGGCCAGGCT GGAGAGGAGC AGCAGCAAGA 
     2161 GCCTGGAGCG GGAACTGGCC ACCCCGGACT GGGCAGAACG GCAGCTGGCC CAAGGAGGCC 
     2221 TGGCTGAGGT GCTGTTGGCT GCCAAGGAGC ACCGGCGGCC GCGTGAGACA CGAGTGATTG 
     2281 CTGTGCTGGG CAAAGCTGGT CAGGGCAAGA GCTATTGGGC TGGGGCAGTG AGCCGGGCCT 
     2341 GGGCTTGTGG CCGGCTTCCC CAGTACGACT TTGTCTTCTC TGTCCCCTGC CATTGCTTGA 
     2401 ACCGTCCGGG GGATGCCTAT GGCCTGCAGG ATCTGCTCTT CTCCCTGGGC CCACAGCCAC 
     2461 TCGTGGCGGC CGATGAGGTT TTCAGCCACA TCTTGAAGAG ACCTGACCGC GTTCTGCTCA 
     2521 TCCTAGACGG CTTCGAGGAG CTGGAAGCGC AAGATGGCTT CCTGCACAGC ACGTGCGGAC 
     2581 CGGCACCGGC GGAGCCCTGC TCCCTCCGGG GGCTGCTGGC CGGCCTTTTC CAGAAGAAGC 
     2641 TGCTCCGAGG TTGCACCCTC CTCCTCACAG CCCGGCCCCG GGGCCGCCTG GTCCAGAGCC 
     2701 TGAGCAAGGC CGACGCCCTA TTTGAGCTGT CCGGCTTCTC CATGGAGCAG GCCCAGGCAT 
     2761 ACGTGATGCG CTACTTTGAG AGCTCAGGGA TGACAGAGCA CCAAGACAGA GCCCTGACGC 
     2821 TCCTCCGGGA CCGGCCACTT CTTCTCAGTC ACAGCCACAG CCCTACTTTG TGCCGGGCAG 
     2881 TGTGCCAGCT CTCAGAGGCC CTGCTGGAGC TTGGGGAGGA CGCCAAGCTG CCCTCCACGC 
     2941 TCACGGGACT CTATGTCGGC CTGCTGGGCC GTGCAGCCCT CGACAGCCCC CCCGGGGCCC 
     3001 TGGCAGAGCT GGCCAAGCTG GCCTGGGAGC TGGGCCGCAG ACATCAAAGT ACCCTACAGG 
     3061 AGGACCAGTT CCCATCCGCA GACGTGAGGA CCTGGGCGAT GGCCAAAGGC TTAGTCCAAC 
     3121 ACCCACCGCG GGCCGCAGAG TCCGAGCTGG CCTTCCCCAG CTTCCTCCTG CAATGCTTCC 
     3181 TGGGGGCCCT GTGGCTGGCT CTGAGTGGCG AAATCAAGGA CAAGGAGCTC CCGCAGTACC 
     3241 TAGCATTGAC CCCAAGGAAG AAGAGGCCCT ATGACAACTG GCTGGAGGGC GTGCCACGCT 
     3301 TTCTGGCTGG GCTGATCTTC CAGCCTCCCG CCCGCTGCCT GGGAGCCCTA CTCGGGCCAT 
     3361 CGGCGGCTGC CTCGGTGGAC AGGAAGCAGA AGGTGCTTGC GAGGTACCTG AAGCGGCTGC 
     3421 AGCCGGGGAC ACTGCGGGCG CGGCAGCTGC TGGAGCTGCT GCACTGCGCC CACGAGGCCG 
     3481 AGGAGGCTGG AATTTGGCAG CACGTGGTAC AGGAGCTCCC CGGCCGCCTC TCTTTTCTGG 
     3541 GCACCCGCCT CACGCCTCCT GATGCACATG TACTGGGCAA GGCCTTGGAG GCGGCGGGCC 
     3601 AAGACTTCTC CCTGGACCTC CGCAGCACTG GCATTTGCCC CTCTGGATTG GGGAGCCTCG 
     3661 TGGGACTCAG CTGTGTCACC CGTTTCAGGG CTGCCTTGAG CGACACGGTG GCGCTGTGGG 
     3721 AGTCCCTGCA GCAGCATGGG GAGACCAAGC TACTTCAGGC AGCAGAGGAG AAGTTCACCA 
     3781 TCGAGCCTTT CAAAGCCAAG TCCCTGAAGG ATGTGGAAGA CCTGGGAAAG CTTGTGCAGA 
     3841 CTCAGAGGAC GAGAAGTTCC TCGGAAGACA CAGCTGGGGA GCTCCCTGCT GTTCGGGACC 
     3901 TAAAGAAACT GGAGTTTGCG CTGGGCCCTG TCTCAGGCCC CCAGGCTTTC CCCAAACTGG 
     3961 TGCGGATCCT CACGGCCTTT TCCTCCCTGC AGCATCTGGA CCTGGATGCG CTGAGTGAGA 
     4021 ACAAGATCGG GGACGAGGGT GTCTCGCAGC TCTCAGCCAC CTTCCCCCAG CTGAAGTCCT 
     4081 TGGAAACCCT CAATCTGTCC CAGAACAACA TCACTGACCT GGGTGCCTAC AAACTCGCCG 
     4141 AGGCCCTGCC TTCGCTCGCT GCATCCCTGC TCAGGCTAAG CTTGTACAAT AACTGCATCT 
     4201 GCGACGTGGG AGCCGAGAGC TTGGCTCGTG TGCTTCCGGA CATGGTGTCC CTCCGGGTGA 
     4261 TGGACGTCCA GTACAACAAG TTCACGGCTG CCGGGGCCCA GCAGCTCGCT GCCAGCCTTC 
     4321 GGAGGTGTCC TCATGTGGAG ACGCTGGCGA TGTGGACGCC CACCATCCCA TTCAGTGTCC 
     4381 AGGAACACCT GCAACAACAG GATTCACGGA TCAGCCTGAG AACGCGTACG CGGCCGCTCG 
     4441 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4501 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5221 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5281 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5341 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5401 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5461 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5521 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5581 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5641 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5701 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5761 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5941 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6001 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6061 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6121 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6181 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6241 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6301 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6361 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6421 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6481 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6541 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6601 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6661 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6721 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6781 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6841 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6901 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6961 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7021 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7081 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7141 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7201 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7261 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7321 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7381 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7441 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7501 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7681 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7741 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7801 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7861 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7921 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7981 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8041 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8101 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8161 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8221 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//