LOCUS       RC239993                8271 bp    DNA     circular  SYN 
DEFINITION  SIN3B (myc-DDK-tagged) - Human SIN3 transcription regulator family member B (SIN3B), transcript variant 2
ACCESSION   NM_001297595
VERSION     NM_001297595.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="SIN3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCACGCT GGCGGTGGCA GCGGTGGCAG CGGTGCCGGC GGCCCCGCGG 
     1081 GCCGGGGGCT GAGCGGCGCC CGCTGGGGTC GCTCGGGCTC CGCAGGCCAC GAGAAGCTGC 
     1141 CGGTGCACGT AGAAGACGCC CTCACCTATC TGGACCAGGT GAAGATCCGC TTTGGCAGCG 
     1201 ACCCTGCCAC CTACAACGGC TTCCTGGAGA TCATGAAGGA GTTCAAAAGC CAGAGCATCG 
     1261 ATACTCCTGG AGTCATCAGA CGTGTCTCGC AGCTCTTCCA CGAGCACCCT GACCTCATTG 
     1321 TTGGATTCAA CGCTTTTCTT CCCCTCGGAT ATAGAATAGA CATTCCCAAG AATGGCAAGT 
     1381 TAAACATACA GTCGCCTCTG ACAAGCCAGG AGAATTCGCA CAACCACGGG GACGGTGCAG 
     1441 AGGACTTCAA GCAGCAGGTG CCGTATAAAG AGGACAAACC CCAGGTGCCC CTGGAGTCCG 
     1501 ATTCCGTGGA ATTCAACAAC GCCATCAGCT ATGTGAATAA GATTAAAACC CGCTTCCTAG 
     1561 ACCACCCAGA AATCTACAGG TCATTCCTGG AGATCCTGCA CACGTACCAG AAGGAGCAGC 
     1621 TGAACACGAG GGGCCGGCCA TTCCGAGGCA TGTCTGAAGA GGAGGTGTTC ACCGAGGTGG 
     1681 CCAACCTCTT CCGGGGCCAG GAGGACCTGC TCTCAGAGTT TGGACAGTTC CTGCCCGAAG 
     1741 CCAAGCGGTC TCTGTTCACA GGAAACGGGC CGTGCGAGAT GCACAGCGTG CAGAAGAACG 
     1801 AGCACGACAA GACCCCGGAG CACAGCAGGA AGCGCTCCCG GCCCTCGCTC CTCCGCCCCG 
     1861 TGTCTGCACC CGCCAAGAAA AAAATGAAAC TTCGTGGTAC CAAAGACCTG TCCATCGCTG 
     1921 CAGTGGGGAA GTACGGGACT CTGCAGGAAT TTTCTTTCTT TGACAAGGTC CGCCGGGTGC 
     1981 TGAAGAGCCA GGAGGTGTAT GAAAACTTCC TCCGCTGCAT CGCACTCTTC AACCAGGAGC 
     2041 TGGTGTCTGG CTCTGAGCTC CTGCAGCTCG TCAGCCCATT TCTGGGGAAA TTTCCAGAAC 
     2101 TCTTTGCACA GTTCAAGTCC TTCCTGGGGG TAAAAGAGCT GTCCTTCGCG CCACCCATGA 
     2161 GCGACAGATC CGGGGACGGG ATAAGCCGGG AAATTGATTA TGCATCCTGC AAGCGCATAG 
     2221 GATCCAGCTA CCGGGCACTC CCCAAAACCT ACCAGCAGCC CAAGTGCAGT GGGAGGACAG 
     2281 CCATCTGCAA GGAGGTACTG AACGACACCT GGGTCTCCTT CCCTTCCTGG TCTGAGGACT 
     2341 CCACGTTCGT CAGCTCCAAG AAGACACCGT ACGAGGAGCA GCTTCACCGC TGTGAGGACG 
     2401 AGCGCTTCGA GTTAGACGTT GTCCTGGAGA CGAACCTGGC CACAATCCGT GTGTTGGAAA 
     2461 GTGTGCAGAA GAAGCTGTCT CGGATGGCGC CGGAAGACCA GGAGAAGTTC CGGCTGGACG 
     2521 ACTCCCTGGG AGGCACGTCG GAGGTGATCC AGCGCCGTGC CATTTATCGC ATCTATGGCG 
     2581 ACAAGGCCCC GGAGATCATC GAGAGCCTCA AGAAGAACCC TGTCACCGCT GTCCCCGTTG 
     2641 TCCTGAAAAG ACTGAAGGCC AAGGAAGAGG AGTGGCGGGA GGCCCAGCAG GGCTTCAACA 
     2701 AGATCTGGCG GGAGCAGTAT GAGAAGGCGT ACCTCAAGTC CCTTGACCAC CAGGCTGTGA 
     2761 ACTTCAAGCA GAACGACACC AAGGCCCTGC GCTCCAAGAG CTTGCTCAAC GAGATCGAGA 
     2821 GCGTCTACGA CGAGCACCAG GAGCAGCACT CGGAGGGCCG CAGTGCCCCC TCTAGCGAGC 
     2881 CGCACCTCAT CTTTGTGTAC GAGGACCGGC AGATCCTGGA GGACGCAGCA GCGCTCATCA 
     2941 GCTACTACGT GAAGCGGCAG CCGGCCATCC AGAAGGAGGA CCAGGGCACC ATCCACCAGC 
     3001 TGCTGCACCA GTTCGTGCCC AGCCTCTTCT TCTCTCAGCA GCTGGACCTG GGCGCCTCCG 
     3061 AGGAGTCAGC TGATGAGGAC CGGGACAGCC CCCAGGGGCA GACCACAGAC CCCAGTGAGC 
     3121 GGAAGAAGCC GGCGCCAGGA CCCCACAGTA GCCCCCCAGA GGAGAAGGGG GCCTTCGGGG 
     3181 ATGCCCCGGC CACTGAGCAG CCACCCCTGC CGCCCCCAGC CCCGCACAAG CCCCTGGACG 
     3241 ATGTCTACAG CCTATTTTTT GCCAACAACA ACTGGTACTT CTTCCTGCGC CTGCACCAGA 
     3301 CCCTGTGCTC CAGGCTGCTG AAGATCTACC GCCAGGCGCA GAAGCAGCTT CTGGAGTATC 
     3361 GGACCGAGAA GGAGCGGGAG AAGCTGCTGT GTGAGGGCCG CAGGGAGAAG GGCAGCGACC 
     3421 CCGCCATGGA GCTGCGGCTG AAGCAGCCCA GTGAAGTGGA GCTGGAGGAG TACTACCCGG 
     3481 CCTTCCTGGA CATGGTGCGG AGCCTGCTGG AGGGCAGCAT CGACCCCACG CAGTACGAGG 
     3541 ACACCCTACG CGAGATGTTC ACCATCCATG CCTACGTGGG CTTCACCATG GACAAGCTGG 
     3601 TGCAGAACAT TGCGCGGCAG CTGCACCACC TCGTGAGCGA TGACGTCTGC CTGAAGGTGG 
     3661 TGGAGCTCTA CCTGAACGAG AAGAAGCGGG GTGCCGCTGG TGGGAACCTG TCCTCCCGCT 
     3721 GCGTCCGCGC TGCTAGGGAG ACCAGCTACC AGTGGAAGGC TGAGCGCTGC ATGGCCGACG 
     3781 AGAACTGCTT CAAGGTGATG TTCCTGCAGC GCAAAGGGCA GGTGATCATG ACCATCGAGC 
     3841 TCCTGGACAC CGAGGAGGCC CAGACGGAGG ACCCTGTGGA GGTCCAGCAC CTGGCTCGGT 
     3901 ACGTGGAGCA GTATGTGGGG ACCGAGGGCG CGTCCAGCTC GCCCACTGAG GGCTTCCTCC 
     3961 TGAAACCTGT GTTCCTGCAG AGGAACCTCA AGAAGTTCCG CCGCCGGTGG CAGAGCGAGC 
     4021 AGGCGCGGGC CCTGCGCGGT GAGGCCAGGA GCTCCTGGAA GCGGCTGGTG GGCGTGGAGA 
     4081 GCGCCTGCGA CGTGGACTGC CGCTTCAAGC TCAGCACTCA CAAGATGGTG TTCATCGTGA 
     4141 ACTCCGAGGA CTACATGTAC CGTCGCGGGA CCCTCTGCCG GGCCAAGCAG GTGCAGCCCC 
     4201 TGGTCCTGCT CCGCCACCAC CAGCACTTTG AGGAGTGGCA CAGCCGCTGG CTGGAGGACA 
     4261 ATGTGACGGT GGAGGCGGCT AGCCTGGTGC AGGACTGGCT GATGGGTGAG GAGGACGAGG 
     4321 ACATGGTACC CTGCAAGACG CTGTGTGAGA CAGTGCACGT GCACGGCCTG CCCGTGACCC 
     4381 GCTACCGCGT GCAGTACAGC CGCCGCCCGG CCTCGCCCAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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