LOCUS       RC239991                8268 bp    DNA     circular  SYN 
DEFINITION  UPF1 (myc-DDK-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant 1
ACCESSION   NM_001297549
VERSION     NM_001297549.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4425..4516 
                     /label="Myc-DDK"  
     misc_feature    4546..5131 
                     /label="PolyA signal"  
     misc_feature    4603..4622 
                     /label="Restriction Enzyme"  
     misc_feature    5407..6079 
                     /label="ColE1"  
     misc_feature    6509..7303 
                     /label="Kan/Neo"  
     misc_feature    7338..7640 
                     /label="SV40 ori"  
     misc_feature    7726..8203 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4407..4413 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4407 
                     /label="UPF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGTGGAG GCGTACGGGC CCAGCTCGCA GACTCTCACT TTCCTGGACA 
     1081 CGGAGGAGGC CGAGCTGCTT GGCGCCGACA CACAGGGCTC CGAGTTCGAG TTCACCGACT 
     1141 TTACTCTTCC TAGCCAGACG CAGACGCCCC CCGGCGGCCC CGGCGGCCCG GGCGGTGGCG 
     1201 GCGCGGGAGG CCCGGGCGGC GCGGGCGCGG GCGCTGCGGC GGGACAGCTC GACGCGCAGG 
     1261 TTGGGCCCGA AGGCATCCTG CAGAACGGGG CTGTGGACGA CAGTGTAGCC AAGACCAGCC 
     1321 AGTTGTTGGC TGAGTTGAAC TTCGAGGAAG ATGAAGAAGA CACCTATTAC ACGAAGGACC 
     1381 TCCCCATACA CGCCTGCAGT TACTGTGGAA TACACGATCC TGCCTGCGTG GTTTACTGTA 
     1441 ATACCAGCAA GAAGTGGTTC TGCAACGGAC GTGGAAATAC TTCTGGCAGC CACATTGTAA 
     1501 ATCACCTTGT GAGGGCAAAA TGCAAAGAGG TGACCCTGCA CAAGGACGGG CCCCTGGGGG 
     1561 AGACAGTCCT GGAGTGCTAC AACTGCGGCT GTCGCAACGT CTTCCTCCTC GGCTTCATCC 
     1621 CGGCCAAAGC TGACTCAGTG GTGGTGCTGC TGTGCAGGCA GCCCTGTGCC AGCCAGAGCA 
     1681 GCCTCAAGGA CATCAACTGG GACAGCTCGC AGTGGCAGCC GCTGATCCAG GACCGCTGCT 
     1741 TCCTGTCCTG GCTGGTCAAG ATCCCCTCCG AGCAGGAGCA GCTGCGGGCA CGCCAGATCA 
     1801 CGGCACAGCA GATCAACAAG CTGGAGGAGC TGTGGAAGGA AAACCCTTCT GCCACGCTGG 
     1861 AGGACCTGGA GAAGCCGGGG GTGGACGAGG AGCCGCAGCA TGTCCTCCTG CGGTACGAGG 
     1921 ACGCCTACCA GTACCAGAAC ATATTCGGGC CCCTGGTCAA GCTGGAGGCC GACTACGACA 
     1981 AGAAGCTGAA GGAGTCCCAG ACTCAAGATA ACATCACTGT CAGGTGGGAC CTGGGCCTTA 
     2041 ACAAGAAGAG AATCGCCTAC TTCACTTTGC CCAAGACTGA CTCTGGTAAT GAGGATTTAG 
     2101 TCATAATTTG GTTAAGAGAC ATGCGGCTCA TGCAGGGGGA TGAGATATGC CTGCGGTACA 
     2161 AAGGGGACCT TGCGCCCCTG TGGAAAGGGA TCGGCCACGT CATCAAGGTC CCTGATAATT 
     2221 ATGGCGATGA GATCGCCATT GAGCTGCGGA GCAGCGTGGG TGCACCTGTG GAGGTGACTC 
     2281 ACAACTTCCA GGTGGATTTT GTGTGGAAGT CGACCTCCTT TGACAGGATG CAGAGCGCAT 
     2341 TGAAAACGTT TGCCGTGGAT GAGACCTCGG TGTCTGGCTA CATCTACCAC AAGCTGTTGG 
     2401 GCCACGAGGT GGAGGACGTA ATCATCAAGT GCCAGCTGCC CAAGCGCTTC ACGGCGCAGG 
     2461 GCCTCCCCGA CCTCAACCAC TCCCAGGTTT ATGCCGTGAA GACTGTGCTG CAAAGACCAC 
     2521 TGAGCCTGAT CCAGGGCCCG CCAGGCACGG GGAAGACGGT GACGTCGGCC ACCATCGTCT 
     2581 ACCACCTGGC CCGGCAAGGC AACGGGCCGG TGCTGGTGTG TGCTCCGAGC AACATCGCCG 
     2641 TGGACCAGCT AACGGAGAAG ATCCACCAGA CGGGGCTAAA GGTCGTGCGC CTCTGCGCCA 
     2701 AGAGCCGTGA GGCCATCGAC TCCCCGGTGT CTTTTCTGGC CCTGCACAAC CAGATCAGGA 
     2761 ACATGGACAG CATGCCTGAG CTGCAGAAGC TGCAGCAGCT GAAAGACGAG ACTGGGGAGC 
     2821 TGTCGTCTGC CGACGAGAAG CGGTACCGGG CCTTGAAGCG CACCGCAGAG AGAGAGCTGC 
     2881 TGATGAACGC AGATGTCATC TGCTGCACAT GTGTGGGCGC CGGTGACCCG AGGCTGGCCA 
     2941 AGATGCAGTT CCGCTCCATT TTAATCGACG AAAGCACCCA GGCCACCGAG CCGGAGTGCA 
     3001 TGGTTCCCGT GGTCCTCGGG GCCAAGCAGC TGATCCTTGT AGGCGACCAC TGCCAGCTGG 
     3061 GCCCAGTGGT GATGTGCAAG AAGGCGGCCA AGGCCGGGCT GTCACAGTCG CTCTTCGAGC 
     3121 GCCTGGTGGT GCTGGGCATC CGGCCCATCC GCCTGCAGGT CCAGTACCGG ATGCACCCTG 
     3181 CACTCAGCGC CTTCCCATCC AACATCTTCT ACGAGGGCTC CCTCCAGAAT GGTGTCACTG 
     3241 CAGCGGATCG TGTGAAGAAG GGATTTGACT TCCAGTGGCC CCAACCCGAT AAACCGATGT 
     3301 TCTTCTACGT GACCCAGGGC CAAGAGGAGA TTGCCAGCTC GGGCACCTCC TACCTGAACA 
     3361 GGACCGAGGC TGCGAACGTG GAGAAGATCA CCACGAAGTT GCTGAAGGCA GGCGCCAAGC 
     3421 CGGACCAGAT TGGCATCATC ACGCCCTACG AGGGCCAGCG CTCCTACCTG GTGCAGTACA 
     3481 TGCAGTTCAG CGGCTCCCTG CACACCAAGC TCTACCAGGA GGTGGAGATC GCCAGTGTGG 
     3541 ACGCCTTTCA GGGACGCGAG AAGGACTTCA TCATCCTGTC CTGTGTGCGG GCCAACGAGC 
     3601 ACCAAGGCAT TGGCTTTTTA AATGACCCCA GGCGTCTGAA CGTGGCCCTG ACCAGAGCAA 
     3661 GGTATGGCGT CATCATTGTG GGCAACCCGA AGGCACTATC AAAGCAGCCG CTCTGGAACC 
     3721 ACCTGCTGAA CTACTATAAG GAGCAGAAGG TGCTGGTGGA GGGGCCGCTC AACAACCTGC 
     3781 GTGAGAGCCT CATGCAGTTC AGCAAGCCAC GGAAGCTGGT CAACACTATC AACCCGGGAG 
     3841 CCCGCTTCAT GACCACAGCC ATGTATGATG CCCGGGAGGC CATCATCCCA GGCTCCGTCT 
     3901 ATGATCGGAG CAGCCAGGGC CGGCCTTCCA GCATGTACTT CCAGACCCAT GACCAGATTG 
     3961 GCATGATCAG TGCCGGCCCT AGCCACGTGG CTGCCATGAA CATTCCCATC CCCTTCAACC 
     4021 TGGTCATGCC ACCCATGCCA CCGCCTGGCT ATTTTGGACA AGCCAACGGG CCTGCTGCAG 
     4081 GGCGAGGCAC CCCGAAAGGC AAGACTGGTC GTGGGGGACG CCAGAAGAAC CGCTTTGGGC 
     4141 TTCCTGGACC CAGCCAGACT AACCTCCCCA ACAGCCAAGC CAGCCAGGAT GTGGCGTCAC 
     4201 AGCCCTTCTC TCAGGGCGCC CTGACGCAGG GCTACATCTC CATGAGCCAG CCTTCCCAGA 
     4261 TGAGCCAGCC CGGCCTCTCC CAGCCGGAGC TGTCCCAGGA CAGTTACCTT GGTGACGAGT 
     4321 TTAAATCACA AATCGACGTG GCGCTCTCAC AGGACTCCAC GTACCAGGGA GAGCGGGCTT 
     4381 ACCAGCATGG CGGGGTGACG GGGCTGTCCC AGTATACGCG TACGCGGCCG CTCGAGCAGA 
     4441 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4501 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5221 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5281 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5341 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5401 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5461 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5521 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5581 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5641 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5701 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5761 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5821 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5881 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5941 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6001 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6061 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6121 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6181 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6241 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6301 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6361 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6421 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6481 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6541 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6601 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6661 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6721 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6781 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6841 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6901 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6961 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7021 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7081 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7141 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7201 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7261 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7321 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7381 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7441 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7501 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7681 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7741 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7801 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7861 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7921 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7981 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8041 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8101 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8161 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8221 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//