LOCUS       RC239986                8256 bp    DNA     circular  SYN 
DEFINITION  USP25 (myc-DDK-tagged) - Human ubiquitin specific peptidase 25 (USP25), transcript variant 1
ACCESSION   NM_001283041
VERSION     NM_001283041.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="USP25"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGTGGAG CAGAACGTGC TGCAGCAGAG CGCGGCGCAG AAGCACCAGC 
     1081 AGACGTTTTT GAATCAACTG AGAGAAATTA CGGGGATTAA TGACACCCAG ATACTACAGC 
     1141 AAGCCTTGAA GGATAGTAAT GGAAACTTGG AATTAGCAGT GGCTTTCCTT ACTGCGAAGA 
     1201 ATGCTAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCACTT CCTGGCAATG 
     1261 ATAGATACAT CAGTGTGGGA AGCCAAGCAG ATACAAATGT GATTGATCTC ACTGGAGATG 
     1321 ATAAAGATGA TCTTCAGAGA GCAATTGCCT TGAGTTTGGC CGAATCAAAC AGGGCATTCA 
     1381 GGGAGACTGG AATAACTGAT GAGGAACAAG CCATTAGCAG AGTTCTTGAA GCCAGCATAG 
     1441 CAGAGAATAA AGCATGTTTG AAGAGGACAC CTACAGAAGT TTGGAGGGAT TCTCGAAACC 
     1501 CTTATGATAG AAAAAGACAG GACAAAGCTC CCGTTGGGCT AAAGAATGTT GGCAATACTT 
     1561 GTTGGTTTAG TGCTGTTATT CAGTCATTAT TTAATCTTTT GGAATTTAGA AGATTAGTTC 
     1621 TGAATTACAA GCCTCCATCA AATGCTCAAG ATTTACCCCG AAACCAAAAG GAACATCGGA 
     1681 ATTTGCCTTT TATGCGTGAG CTGAGGTATC TATTTGCACT TCTTGTTGGT ACCAAAAGGA 
     1741 AGTATGTTGA TCCATCAAGA GCAGTTGAAA TTCTTAAGGA TGCTTTCAAA TCAAATGACT 
     1801 CACAGCAGCA AGATGTGAGT GAGTTTACAC ACAAATTATT AGATTGGTTA GAAGATGCCT 
     1861 TCCAAATGAA AGCTGAAGAG GAGACGGATG AAGAGAAGCC AAAGAACCCC ATGGTAGAGT 
     1921 TGTTCTATGG CAGATTCCTG GCTGTGGGAG TACTTGAAGG TAAAAAATTT GAAAACACTG 
     1981 AAATGTTTGG TCAGTACCCA CTTCAGGTCA ATGGGTTCAA AGATCTGCAT GAGTGCCTAG 
     2041 AAGCTGCAAT GATTGAAGGA GAAATTGAGT CTTTACATTC AGAGAATTCA GGAAAATCAG 
     2101 GCCAAGAGCA TTGGTTTACT GAATTACCAC CTGTGTTAAC ATTTGAATTG TCAAGATTTG 
     2161 AATTTAATCA GGCATTGGGA AGACCAGAAA AAATTCACAA CAAATTAGAA TTTCCCCAAG 
     2221 TTTTATATTT GGACAGATAC ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG 
     2281 AGATCAAGAG ACTGAAAGAT TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA 
     2341 GCTATGGTTC CGGTCCCAAA CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT 
     2401 TTGCCTCAAG TAAACCTGTT TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC 
     2461 CTAGTGGTTC CATACCATCA CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT 
     2521 CTTCAGAACT GCCAAGCACA TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA 
     2581 TACACAAACC ATTTACTCAG TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA 
     2641 GGCACATAAC GGAGGAAGAA CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG 
     2701 AAATAGAAAA TGACACCAGA GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG 
     2761 AATTAATGTA CTCTGACAAA TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG 
     2821 TTCACGAAGG CCAAGCTAAT GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA 
     2881 GCAGATGGAT GAAGTACAAT GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA 
     2941 GGGACTCTTT TGGTGGTTAT AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA 
     3001 AGGCACAGTT CCTAATACAA GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA 
     3061 TAGAAACATT ACCACCGGAT TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA 
     3121 AAGAACTAGA AGAATGGGAT GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG 
     3181 CGTCTCAGAA ATTGAGAGAG TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC 
     3241 CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA 
     3301 TTCAAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC 
     3361 AGTCGAAACC TGAAAATACT ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG 
     3421 CGATCCCTCA TGTAGGGAAA TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA 
     3481 TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT 
     3541 ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT 
     3601 TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG 
     3661 TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC 
     3721 AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC 
     3781 AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC 
     3841 ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT 
     3901 TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA 
     3961 AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA 
     4021 GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG 
     4081 ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT 
     4141 TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC 
     4201 GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT 
     4261 TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT 
     4321 TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA 
     4381 GTCGAACTCC TGCTGATGGA AGAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//