LOCUS       RC239974                8196 bp    DNA     circular  SYN 
DEFINITION  KDM6A (myc-DDK-tagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 6
ACCESSION   NM_001291421
VERSION     NM_001291421.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4353..4444 
                     /label="Myc-DDK"  
     misc_feature    4474..5059 
                     /label="PolyA signal"  
     misc_feature    4531..4550 
                     /label="Restriction Enzyme"  
     misc_feature    5335..6007 
                     /label="ColE1"  
     misc_feature    6437..7231 
                     /label="Kan/Neo"  
     misc_feature    7266..7568 
                     /label="SV40 ori"  
     misc_feature    7654..8131 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4335..4341 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4335 
                     /label="KDM6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCACACT GTAGATCTCC TGGGAGATAA AGCCACCAAG GAAAGCTATG 
     1081 CTATTCAGTA TCTCCAAAAG TCCTTGGAAG CAGATCCTAA TTCTGGCCAG TCCTGGTATT 
     1141 TCCTCGGAAG GTGCTATTCA AGTATTGGGA AAGTTCAGGA TGCCTTTATA TCTTACAGGC 
     1201 AGTCTATTGA TAAATCAGAA GCAAGTGCAG ATACATGGTG TTCAATAGGT GTGCTATATC 
     1261 AGCAGCAAAA TCAGCCCATG GATGCTTTAC AGGCCTATAT TTGTGCTGTA CAATTGGACC 
     1321 ATGGCCATGC TGCAGCCTGG ATGGACCTAG GCACTCTCTA TGAATCCTGC AACCAGCCTC 
     1381 AGGATGCCAT TAAATGCTAC TTAAATGCAA CTAGAAGCAA AAGTTGTAGT AATACCTCTG 
     1441 CACTTGCAGC ACGAATTAAG TATTTACAGA ATACTTCTGA CAATTGGAGT GGTGGACATG 
     1501 CTGTGTCACA TCCTCCAGTA CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT 
     1561 TACAGCATTT GGAACAGCTC CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA 
     1621 TGCTGGAACA GCTGGAAAGT CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG 
     1681 GAGTTGCACA GGTACGATCT ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC 
     1741 CTACAAACTC AGTCTCTGGC CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT 
     1801 CTCAGCCTGG AGTCCGTCCT GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG 
     1861 CAGGCCATGT TCCCTGTAGC ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG 
     1921 GCAATAATCA TATAACAGGA AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG 
     1981 CACTCACTCT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA 
     2041 ACCAACTATC TAACTCCACT CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC 
     2101 CTAATGGTGA ACGACCTCTC TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT 
     2161 CTGGTATTCA GAATCAGAAC GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG 
     2221 CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT 
     2281 TAACCAAAGA GAGCAAGCCT TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA 
     2341 CTGGAGAGAC ACCTAACAGC ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA 
     2401 TGACGGCAGA TGCTGTTTGC AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA 
     2461 GTTCAGACAA TCCTCAGCTC TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA 
     2521 CTGGAACCTG TGACAAAGTC AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT 
     2581 CTGTTGCCTC TTCACCATCT TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG 
     2641 AGCAGACAAC CACAAACAGT GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA 
     2701 TTAATGGAGA AGGGATGGAA GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA 
     2761 ACCACAAACC TAGTCCACAG ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG 
     2821 CAGAAGTTCT GAAGGCATGC AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT 
     2881 TGTTGGATAA ATGTCCACCT CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG 
     2941 ACAAGTTGAA TCCACCTACA CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC 
     3001 CTCCATTACA TCAATTTTGT ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG 
     3061 CTGGAGCTCT TAAGTTAGAC CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA 
     3121 ATGAACATAT GGTAGAAGTG AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC 
     3181 CCACTGGAAC AAAGAAAATC TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA 
     3241 AATATGCACA GTACCAGGCC TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA 
     3301 GAAGTCATCA TAAAGACCAC TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA 
     3361 GGAGGAAAGG ACCCTTTAAA ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA 
     3421 AAAAGTGGAA GTTGCAGCTA CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT 
     3481 CAGCAGGAAA TCTTCTAAGC CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC 
     3541 TATACATGAA AGTTCCAGGG AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT 
     3601 CAGTTAACAT AAATATTGGC CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT 
     3661 GGGGTGTTCT GAATGACTTC TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT 
     3721 GGCCCAATCT TGAAGATCTT TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC 
     3781 CTGGAGATTT GGTCTGGATA AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT 
     3841 GCAACAACAT TGCTTGGAAT GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG 
     3901 AACGGTACGA ATGGAACAAA TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT 
     3961 CCTGGAATAT GGCACGAAAT ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT 
     4021 ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG 
     4081 GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC TGTAGCATTT 
     4141 GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA CGAAAGACCT 
     4201 ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA AACTTTGTGG 
     4261 TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC 
     4321 CTCCATTACC ATCCGCCTCA TCTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4381 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4441 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4501 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4561 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4621 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4681 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4741 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4801 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4861 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4921 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4981 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5041 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5101 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5161 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5221 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5281 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5341 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5401 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5461 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5521 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5581 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5641 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5701 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5761 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5821 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5881 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5941 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6001 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6061 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6121 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6181 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6241 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6301 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6361 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6421 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6481 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6541 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6601 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6661 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6721 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6781 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6841 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6901 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6961 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7021 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7081 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7141 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7201 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7261 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7321 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7381 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7441 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7621 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7681 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7741 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7801 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7861 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7921 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7981 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8041 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8101 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8161 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//