LOCUS       RC239972                8193 bp    DNA     circular  SYN 
DEFINITION  UBN1 (myc-DDK-tagged) - Human ubinuclein 1 (UBN1), transcript variant 3
ACCESSION   NM_001288656
VERSION     NM_001288656.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4350..4441 
                     /label="Myc-DDK"  
     misc_feature    4471..5056 
                     /label="PolyA signal"  
     misc_feature    4528..4547 
                     /label="Restriction Enzyme"  
     misc_feature    5332..6004 
                     /label="ColE1"  
     misc_feature    6434..7228 
                     /label="Kan/Neo"  
     misc_feature    7263..7565 
                     /label="SV40 ori"  
     misc_feature    7651..8128 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4332..4338 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4332 
                     /label="UBN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGAGCCC CACAGGGTCC AGTTCACCTC TCTCCCAGGT TCCCTGAATC 
     1081 CTGCGTTTTT GAAGAAGTCC CGGAAGGAGG AGGCTGGGGC AGGAGAACAG CATCAGGACT 
     1141 GTGAGCCGGC TGCAGCAGCT GTTCGGATTA CACTCACCCT CTTTGAACCA GATCACAAAC 
     1201 GCTGCCCAGA GTTCTTCTAC CCAGAGCTGG TGAAGAATAT CCGAGGGAAG GTAAAAGGCC 
     1261 TTCAGCCTGG AGATAAGAAG AAAGATCTGT CAGATCCTTT CAATGACGAA GAAAAGGAAA 
     1321 GGCATAAAGT AGAGGCCCTT GCCCGAAAAT TTGAAGAAAA ATACGGTGGA AAGAAACGTA 
     1381 GAAAAGACCG AATACAGGAC TTGATCGATA TGGGGTATGG TTATGATGAA TCCGACTCCT 
     1441 TCATCGATAA CTCTGAGGCG TATGATGAGC TTGTTCCTGC TTCTTTGACT ACGAAGTATG 
     1501 GAGGATTTTA CATTAACTCG GGAACCCTGC AGTTTAGACA AGCATCAGAG TCTGAAGATG 
     1561 ACTTCATTAA AGAAAAGAAG AAAAAATCTC CAAAGAAGCG GAAGTTGAAG GAAGGTGGTG 
     1621 AGAAGATAAA GAAGAAGAAA AAAGATGACA CTTATGACAA GGAGAAGAAA TCGAAAAAGT 
     1681 CCAAGTTTTC CAAAGCCGGC TTCACAGCCC TCAATGCCAG TAAGGAGAAG AAGAAGAAGA 
     1741 AATATTCTGG GGCTTTAAGC GTTAAAGAGA TGCTAAAGAA ATTTCAGAAA GAGAAAGAGG 
     1801 CTCAGAAAAA AAGGGAGGAG GAGCATAAGC CTGTTGCGGT CCCATCAGCG GAAGCTCAGG 
     1861 GCCTGCGGGA ACTGGAGGGT GCCTCTGACC CCTTGCTCTC ACTCTTTGGC TCTACTTCTG 
     1921 ACAACGACTT GCTCCAGGCG GCCACTGCCA TGGACTCGCT GACGGATTTG GACTTGGAGC 
     1981 ATCTGCTCAG TGAGTCTCCA GAAGGAAGTC CCTTCCGAGA TATGGATGAT GGAAGTGATT 
     2041 CCCTTGGGGT GGGATTGGAC CAGGAATTCA GGCAGCCCTC TTCTCTCCCC GAAGGCCTGC 
     2101 CAGCACCCCT GGAGAAGCGC GTTAAGGAGC TGGCTCAGGC TGCCAGAGCT GCTGAGGGGG 
     2161 AGAGCAGACA GAAGTTCTTC ACCCAGGATA TTAATGGAAT CCTATTAGAC ATAGAGGCGC 
     2221 AGACTCGGGA GCTGAGCAGT CAGGTCCGCT CTGGGGTGTA TGCCTATCTT GCGTCATTCC 
     2281 TGCCCTGCAG CAAGGATGCC CTGCTCAAGC GTGCTCGGAA ACTTCACCTC TATGAACAGG 
     2341 GGGGCCGTCT GAAGGAGCCT CTCCAGAAGC TCAAGGAAGC CATTGGCAGG GCGATGCCAG 
     2401 AGCAGATGGC CAAGTACCAG GACGAATGCC AGGCACACAC GCAGGCAAAG GTTGCCAAGA 
     2461 TGCTGGAAGA GGAGAAAGAC AAGGAGCAGA GGGACCGGAT TTGTTCGGAT GAGGAAGAAG 
     2521 ATGAAGAAAA AGGGGGCAGG AGGATAATGG GACCTCGGAA GAAGTTCCAA TGGAATGATG 
     2581 AGATCAGGGA GCTACTGTGC CAGGTGGTGA AGATCAAACT GGAGAGCCAG GACCTGGAGA 
     2641 GGAACAACAA AGCCCAGGCT TGGGAGGACT GTGTGAAGGG CTTTCTGGAT GCGGAAGTCA 
     2701 AGCCCCTCTG GCCCAAAGGC TGGATGCAGG CCAGAACTCT GTTTAAGGAG AGCAGACGAG 
     2761 GCCATGGGCA CCTGACTTCA ATCCTGGCCA AGAAGAAAGT TATGGCCCCT TCTAAAATCA 
     2821 AGGTGAAGGA ATCGTCTACG AAGCCTGATA AAAAGGTTTC TGTCCCATCA GGACAGATTG 
     2881 GTGGCCCCAT TGCTTTGCCC TCAGATCACC AAACAGGAGG CCTGAGTATT GGGGCCTCGA 
     2941 GCAGGGAGCT CCCATCCCAG GCATCGGGCG GCCTTGCTAA CCCTCCTCCT GTCAACCTGG 
     3001 AGGACTCATT GGATGAAGAC TTGATCCGCA ATCCAGCCTC CTCGGTGGAA GCCGTGTCCA 
     3061 AGGAATTGGC TGCATTGAAT AGCAGAGCAG CTGGGAACTC TGAATTCACA CTGCCTGCAC 
     3121 CCTCAAAAGC ACCTGCAGAA AAAGTTGGAG GCGTTTTATG TACAGAAGAA AAAAGGAACT 
     3181 TTGCGAAGCC TAGTCCTTCT GCTCCACCAC CAGCTAGCTC TCTGCAGTCA CCCCTCAATT 
     3241 TTCTGGCAGA ACAGGCTCTG GCACTGGGGC AGTCCTCTCA GGAGAAAAAA CCAGAGAGTT 
     3301 CTGGCTACAA AGAGCTGTCC TGCCAGGCTC CCCTCAATAA GGGCCTGCCA GAAGTACATC 
     3361 AGTCCAAAGC TAAGCACCAC AGCTTGCCAC GGACGTCTCA CGGGCCCCAA GTGGCAGTTC 
     3421 CTGTGCCTGG CCCCCAGGTC AAAGTCTTTC ATGCCGGCAC TCAGCAGCAG AAAAACTTCA 
     3481 CGCCCCCATC TCCATTTGCC AATAAGCTCC AAGGCCCAAA GGCTTCTCCC ACACAGTGTC 
     3541 ATCGTTCCCT CCTGCAGTTA GTGAAGACAG CGGCCAAAGG CCAGGGCTTC CATCCCTCTG 
     3601 CACCAGCCAC CTCAGGAGGC CTGTCAGCCT CCAGCAGCAG CTCTCACAAG ACCCCAGCCT 
     3661 CGTCCTCTTC TGCCCTGAGC CATCCAGCAA AGCCACATTC AGTCAGCTCT GCAGGCTCAT 
     3721 CTTACAAGAA TAATCCCTTT GCCAGCTCAA TCTCCAAACA TGGGGTTTCT TCTGGCAGCT 
     3781 CTTCCTCGGG AGGAACACCA GTCCAGAGTT CTGTTTCTGG GAGCCTGGTC CCTGGCATAC 
     3841 AGCCTCCCTC CGTGGGACAG GCCACCAGCC GACCCGTCCC AAGTTCAGCA GGGAAAAAAA 
     3901 TGCCTGTTTC CCAGAAGTTG ACTCTGGTAG CCCCTCCAGG CGGTCCAAAC GGAGATTCCA 
     3961 GTGGTGGGAC CCAGGGAGTG GCAAAGTTGC TGACCTCGCC GTCCCTAAAG CCCTCTGCAG 
     4021 TTAGTAGTGT GACATCGTCT ACCTCCTTGT CAAAAGGAGC GAGTGGGACT GTGCTGCTGG 
     4081 CCGGCTCCTC TTTGATGGCT TCACCCTACA AATCCAGCAG CCCAAAGCTG TCTGGGGCCA 
     4141 TGAGCTCGAA CTCCTTGGGA ATTATAACCC CTGTCCCTAT TCCTGTCCAT GTGCTCTCCT 
     4201 TCAGCGCTGA CTCCTCTGCC AAAGCAGGGG TCTCCAAGGA TGCCATCGTC ACAGGCCCTG 
     4261 CCCCCGGGTC CTTCCACCAT GGCCTTGGCC ACAGTGCTTC TCAGCTTCAC GGGAAAGGGC 
     4321 CTGCTGTACC ACGGAAATTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4381 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5161 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5221 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5281 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5341 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5461 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5521 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5581 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5641 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5761 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5821 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5881 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5941 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6001 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6061 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6121 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6181 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6241 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6301 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6361 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6421 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6481 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6541 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6601 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6661 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6721 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6781 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6841 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6901 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6961 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7021 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7081 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7141 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7201 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7261 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7321 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7381 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7441 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7621 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7741 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7801 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7861 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7921 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7981 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8041 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8101 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8161 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//