LOCUS       RC239969                8187 bp    DNA     circular  SYN 
DEFINITION  SIK3 (myc-DDK-tagged) - Human SIK family kinase 3 (SIK3), transcript variant 2
ACCESSION   NM_001281748
VERSION     NM_001281748.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4344..4435 
                     /label="Myc-DDK"  
     misc_feature    4465..5050 
                     /label="PolyA signal"  
     misc_feature    4522..4541 
                     /label="Restriction Enzyme"  
     misc_feature    5326..5998 
                     /label="ColE1"  
     misc_feature    6428..7222 
                     /label="Kan/Neo"  
     misc_feature    7257..7559 
                     /label="SV40 ori"  
     misc_feature    7645..8122 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4326..4332 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4326 
                     /label="SIK3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAAAAG GAGGCACGTC GGAAGTTCAA ACAGATCGTC ACAGCTGTCT 
     1081 ATTTTTGTCA CTGTCGGAAC ATTGTTCATC GTGATTTAAA AGCTGAAAAT TTACTTCTGG 
     1141 ATGCCAATCT GAATATCAAA ATAGCAGATT TTGGTTTCAG TAACCTCTTC ACTCCTGGGC 
     1201 AGCTGCTGAA GACCTGGTGT GGCAGCCCTC CCTATGCTGC ACCTGAACTC TTTGAAGGAA 
     1261 AAGAATATGA TGGGCCCAAA GTGGACATCT GGAGCCTTGG AGTTGTCCTC TACGTGCTTG 
     1321 TGTGCGGTGC CCTGCCATTT GATGGAAGCA CACTGCAGAA TCTGCGGGCC CGCGTGCTGA 
     1381 GTGGAAAGTT CCGCATCCCA TTTTTTATGT CCACAGAATG TGAGCATTTG ATCCGCCATA 
     1441 TGTTGGTGTT AGATCCCAAT AAGCGCCTCT CCATGGAGCA GATCTGCAAG CACAAGTGGA 
     1501 TGAAGCTAGG GGACGCCGAT CCCAACTTTG ACAGGTTAAT AGCTGAATGC CAACAACTAA 
     1561 AGGAAGAAAG ACAGGTGGAC CCCCTGAATG AGGATGTCCT CTTGGCCATG GAGGACATGG 
     1621 GACTGGACAA AGAACAGACA CTGCAGTCAT TAAGATCAGA TGCCTATGAT CACTATAGTG 
     1681 CAATCTACAG CCTGCTGTGT GATCGACATA AGAGACATAA AACCCTGCGT CTCGGAGCAC 
     1741 TTCCTAGCAT GCCCCGAGCC CTGGCCTTTC AAGCACCAGT CAATATCCAG GCGGAGCAGG 
     1801 CAGGTACTGC TATGAACATC AGCGTTCCCC AGGTGCAGCT GATCAACCCA GAGAACCAAA 
     1861 TTGTGGAGCC GGATGGGACA CTGAATTTGG ACAGTGATGA GGGTGAAGAG CCTTCCCCTG 
     1921 AAGCATTGGT GCGCTATTTG TCAATGAGGA GGCACACAGT GGGTGTGGCT GACCCACGCA 
     1981 CGGAAGTTAT GGAAGATCTG CAGAAGCTCC TACCTGGCTT TCCTGGAGTC AACCCCCAGG 
     2041 CTCCATTCCT GCAGGTGGCC CCTAATGTGA ACTTCATGCA CAACCTGTTG CCTATGCAAA 
     2101 ACTTGCAACC AACCGGGCAA CTTGAGTACA AGGAGCAGTC TCTCCTACAG CCGCCCACGC 
     2161 TACAGCTGTT GAATGGAATG GGCCCCCTTG GCCGGAGGGC ATCAGATGGA GGAGCCAACA 
     2221 TCCAACTGCA TGCCCAGCAG CTGCTGAAGC GCCCACGGGG ACCCTCTCCG CTTGTCACCA 
     2281 TGACACCAGC AGTGCCAGCA GTTACCCCTG TGGACGAGGA GAGCTCAGAC GGGGAGCCAG 
     2341 ACCAGGAAGC TGTGCAGAGC TCTACCTACA AGGACTCCAA CACTCTGCAC CTCCCTACGG 
     2401 AGCGTTTCTC CCCTGTGCGC CGGTTCTCAG ATGGGGCTGC GAGCATCCAG GCCTTCAAAG 
     2461 CTCACCTGGA AAAAATGGGC AACAACAGCA GCATCAAACA GCTGCAGCAG GAGTGTGAGC 
     2521 AGCTGCAGAA GATGTACGGG GGGCAGATTG ATGAAAGAAC CCTGGAGAAG ACCCAGCAGC 
     2581 AGCATATGTT ATACCAGCAG GAGCAGCACC ATCAAATTCT CCAGCAACAA ATTCAAGACT 
     2641 CTATCTGTCC TCCTCAGCCA TCTCCACCTC TTCAGGCTGC ATGTGAAAAT CAGCCAGCCC 
     2701 TCCTTACCCA TCAGCTCCAG AGGTTAAGGA TTCAGCCTTC AAGCCCACCC CCCAACCACC 
     2761 CCAACAACCA TCTCTTCAGG CAGCCCAGTA ATAGTCCTCC CCCCATGAGC AGTGCCATGA 
     2821 TCCAGCCTCA CGGGGCTGCA TCTTCTTCCC AGTTTCAAGG CTTACCTTCC CGCAGTGCAA 
     2881 TCTTTCAGCA GCAACCTGAG AACTGTTCCT CTCCTCCCAA CGTGGCACTA ACCTGCTTGG 
     2941 GTATGCAGCA GCCTGCTCAG TCACAGCAGG TCACCATCCA AGTCCAAGAG CCTGTTGACA 
     3001 TGCTCAGCAA CATGCCAGGC ACAGCTGCAG GCTCCAGTGG GCGCGGCATC TCCATCAGCC 
     3061 CCAGTGCTGG TCAGATGCAG ATGCAGCACC GTACCAACCT GATGGCCACC CTCAGCTATG 
     3121 GGCACCGTCC CTTGTCCAAG CAGCTGAGTG CTGACAGTGC AGAGGCTCAC AGTGCACATC 
     3181 AGCAGCCGCC ACACTATACC ACGTCGGCAC TACAGCAGGC CCTGCTGTCT CCCACGCCGC 
     3241 CAGACTATAC AAGACACCAG CAGGTACCCC ACATCCTTCA AGGACTGCTT TCTCCCCGGC 
     3301 ATTCGCTCAC CGGCCACTCG GACATCCGGC TGCCCCCAAC AGAGTTTGCA CAGCTCATTA 
     3361 AAAGGCAGCA GCAACAACGG CAGCAGCAGC AGCAACAGCA GCAACAGCAA GAATACCAGG 
     3421 AACTGTTCAG GCACATGAAC CAAGGGGATG CGGGGAGTCT GGCTCCCAGC CTTGGGGGAC 
     3481 AGAGCATGAC AGAGCGCCAG GCTTTATCTT ATCAAAATGC TGACTCTTAT CACCATCACA 
     3541 CCAGCCCCCA GCATCTGCTA CAAATCAGGG CACAAGAATG TGTCTCACAG GCTTCCTCAC 
     3601 CCACCCCGCC CCACGGGTAT GCTCACCAGC CGGCACTGAT GCATTCAGAG AGCATGGAGG 
     3661 AGGACTGCTC GTGTGAGGGG GCCAAGGATG GCTTCCAAGA CAGTAAGAGT TCAAGTACAT 
     3721 TGACCAAAGG TTGCCATGAC AGCCCTCTGC TCTTGAGTAC CGGTGGACCT GGGGACCCTG 
     3781 AATCTTTGCT AGGAACTGTG AGTCATGCCC AAGAATTGGG GATACATCCC TATGGTCATC 
     3841 AGCCAACTGC TGCATTCAGT AAAAATAAGG TGCCCAGCAG AGAGCCTGTC ATAGGGAACT 
     3901 GCATGGATAG AAGTTCTCCA GGACAAGCAG TGGAGCTGCC GGATCACAAT GGGCTCGGGT 
     3961 ACCCAGCACG CCCCTCCGTC CATGAGCACC ACAGGCCCCG GGCCCTCCAG AGACACCACA 
     4021 CGATCCAGAA CAGCGACGAT GCTTATGTAC AGCTGGATAA CTTGCCAGGA ATGAGTCTCG 
     4081 TGGCTGGGAA AGCACTTAGC TCTGCCCGGA TGTCGGATGC AGTTCTCAGT CAGTCTTCGC 
     4141 TCATGGGCAG CCAGCAGTTT CAGGATGGGG AAAATGAGGA ATGTGGGGCA AGCCTGGGAG 
     4201 GTCATGAGCA CCCAGACCTG AGTGATGGCA GCCAGCATTT AAACTCCTCT TGCTATCCAT 
     4261 CTACGTGTAT TACAGACATT CTGCTCAGCT ACAAGCACCC CGAAGTCTCC TTCAGCATGG 
     4321 AGCAGGCAGG CGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4381 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4441 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4501 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4561 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4621 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4681 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4741 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4801 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4861 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4921 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4981 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5041 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5101 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5161 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5221 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5281 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5341 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5401 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5461 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5521 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5581 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5641 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5701 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5761 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5821 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5881 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5941 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6001 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6061 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6121 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6181 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6241 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6301 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6361 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6421 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6481 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6541 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6601 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6661 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6721 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6781 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6841 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6901 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6961 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7021 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7081 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7141 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7201 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7261 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7321 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7381 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7621 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7681 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7741 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7801 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7861 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7921 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7981 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8041 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8101 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8161 ATTTAACAAA AATTTAACGC GAATTTT
//