LOCUS       RC239964                8184 bp    DNA     circular  SYN 
DEFINITION  AP3B2 (myc-DDK-tagged) - Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), transcript variant 1
ACCESSION   NM_001278512
VERSION     NM_001278512.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4341..4432 
                     /label="Myc-DDK"  
     misc_feature    4462..5047 
                     /label="PolyA signal"  
     misc_feature    4519..4538 
                     /label="Restriction Enzyme"  
     misc_feature    5323..5995 
                     /label="ColE1"  
     misc_feature    6425..7219 
                     /label="Kan/Neo"  
     misc_feature    7254..7556 
                     /label="SV40 ori"  
     misc_feature    7642..8119 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4323..4329 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4323 
                     /label="AP3B2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCGCC CCCGCCTACA GCGAAGACAA GGGCGGCTCC GCTGGCCCCG 
     1081 GGGAGCCCGA GTACGGCCAC GACCCCGCGA GCGGCGGCAT CTTCTCCTCC GACTACAAGC 
     1141 GGCATGATGA CCTGAAGGAG ATGCTGGACA CCAACAAGGA TTCTCTCAAG CTGGAGGCCA 
     1201 TGAAGAGGAT TGTGGCGATG ATTGCCCGAG GAAAGAATGC TTCAGACCTG TTTCCCGCGG 
     1261 TGGTGAAGAA CGTGGCCTGT AAGAACATAG AGGTGAAGAA GCTTGTCTAT GTGTACCTGG 
     1321 TACGCTACGC TGAGGAGCAG CAAGACCTGG CCCTGCTGTC CATCTCCACC TTCCAACGTG 
     1381 GCCTAAAGGA TCCCAACCAG CTGATTCGTG CCAGTGCCCT CCGTGTCCTC TCTAGCATCC 
     1441 GTGTGCCCAT CATAGTGCCC ATCATGATGC TAGCTATCAA GGAAGCCGCC TCGGACATGT 
     1501 CACCCTATGT GCGGAAAACA GCTGCCCACG CCATCCCTAA ACTCTACAGT TTGGACTCTG 
     1561 ACCAGAAGGA TCAGCTGATA GAAGTCATTG AGAAGCTTCT GGCTGACAAG ACCACGCTGG 
     1621 TGGCGGGCAG TGTGGTGATG GCCTTTGAGG AGGTCTGCCC GGAGCGCATC GACCTGATTC 
     1681 ACAAAAACTA CCGGAAACTC TGTAACCTGC TGATCGACGT GGAGGAGTGG GGCCAGGTGG 
     1741 TCATCATCAG CATGCTCACC CGCTACGCCC GCACGCAGTT CCTGAGCCCC ACCCAGAACG 
     1801 AATCCCTACT AGAGGAGAAC GCGGAAAAAG CCTTCTACGG CTCAGAGGAG GACGAGGCCA 
     1861 AGGGCGCGGG GTCTGAGGAG ACGGCCGCCG CGGCCGCCCC CTCCCGAAAG CCCTATGTCA 
     1921 TGGACCCCGA CCACCGGCTG CTGCTGCGCA ACACCAAACC CCTGCTGCAG AGCCGCAGCG 
     1981 CCGCGGTGGT GATGGCGGTG GCGCAGCTCT ACTTCCACCT GGCGCCCAAG GCGGAAGTGG 
     2041 GCGTCATCGC CAAGGCGCTG GTGCGCCTGC TGCGCAGCCA CAGTGAGGTG CAGTACGTTG 
     2101 TGCTCCAGAA CGTGGCCACC ATGTCCATCA AGCGCCGGGG TATGTTTGAG CCCTACCTGA 
     2161 AGAGCTTCTA CATCAGGTCC ACCGACCCCA CCCAGATTAA GATCCTGAAG CTGGAAGTGC 
     2221 TGACCAACCT GGCCAATGAG ACCAACATTC CTACTGTCCT ACGGGAATTC CAGACCTATA 
     2281 TTCGCAGCAT GGACAAGGAC TTTGTGGCAG CCACAATCCA GGCCATTGGA CGCTGTGCAA 
     2341 CTAACATCGG CCGAGTCCGT GACACCTGCC TCAATGGCCT GGTGCAGCTG CTGTCCAACC 
     2401 GTGATGAGCT TGTGGTTGCA GAGTCAGTGG TCGTCATTAA GAAATTGCTA CAGATGCAGC 
     2461 CAGCACAACA TGGAGAGATC ATCAAACACT TGGCAAAGCT TACAGACAAC ATCCAGGTGC 
     2521 CCATGGCCCG AGCCAGCATC CTGTGGCTCA TCGGAGAGTA CTGTGAGCAT GTCCCCAGGA 
     2581 TTGCACCTGA TGTCTTAAGA AAAATGGCCA AGTCATTCAC AGCAGAGGAG GATATTGTCA 
     2641 AGCTGCAGGT CATCAACCTG GCAGCCAAGC TCTACCTGAC CAACTCTAAA CAGACCAAGC 
     2701 TGCTGACCCA GTATGTGCTG AGTCTGGCCA AATATGACCA GAACTATGAT ATTCGCGACC 
     2761 GGGCGCGCTT CACCCGGCAG CTCATCGTCC CTTCCGAGCA GGGTGGGGCC CTCAGCCGCC 
     2821 ATGCCAAGAA GCTCTTCCTG GCACCCAAAC CAGCTCCAGT CTTGGAGTCA TCCTTCAAAG 
     2881 ACCGGGACCA CTTCCAGCTG GGCTCACTGT CCCACCTGCT TAATGCCAAG GCCACAGGCT 
     2941 ACCAGGAGCT CCCAGACTGG CCGGAGGAAG CCCCAGACCC ATCTGTGCGC AACGTGGAGG 
     3001 AAGAAGATCT CTCCCTTATT GAGACTCACG TGGGCCTGTT GGGCGAATAC ACTGAGGTAC 
     3061 CTGAATGGAC CAAGTGCTCA AATCGGGAGA AGAGAAAGGA GAAGGAAAAA CCCTTCTACT 
     3121 CGGACTCTGA GGGGGAGTCA GGCCCCACGG AGTCCGCAGA CAGTGACCCT GAGTCTGAGA 
     3181 GTGAATCGGA CAGTAAGAGC AGCAGTGAGA GCGGCTCTGG GGAGTCCAGC AGTGAGTCCG 
     3241 ACAATGAAGA CCAGGATGAG GATGAGGAGA AAGGGAGAGG CAGTGAGAGT GAACAGAGTG 
     3301 AGGAGGATGG TAAGAGGAAG ACAAAGAAGA AGGTGCCAGA GAGAAAAGGA GAAGCGTCAT 
     3361 CCTCTGATGA GGGCAGCGAT TCCAGCAGTA GCTCATCAGA GTCCGAGATG ACATCGGAGT 
     3421 CCGAGGAGGA GCAGTTAGAA CCTGCCTCCT GGAGCAGGAA AACACCTCCC AGCAGCAAAA 
     3481 GTGCTCCTGC AACCAAGGAG ATCTCCCTGC TTGATCTAGA GGATTTCACC CCTCCCAGTG 
     3541 TCCAGCCTGT GTCTCCCCCA GCAATTGTGT CTACCAGTCT GGCTGCTGAC CTGGAGGGCC 
     3601 TGACACTCAC AGACTCCACC CTGGTACCGT CGCTTCTGAG TCCAGTATCG GGTGTTGGGC 
     3661 GGCAGGAGCT GCTGCACCGG GTAGCTGGCG AGGGGCTGGC TGTGGACTAC ACCTTCAGCC 
     3721 GCCAACCTTT CTCCGGGGAT CCCCACATGG TGTCCGTGCA CATCCACTTC TCCAACAGCT 
     3781 CTGATACCCC CATCAAGGGC CTGCATGTGG GCACTCCCAA ACTGCCTGCT GGCATCAGCA 
     3841 TCCAAGAATT TCCCGAAATT GAGTCCCTGG CACCTGGAGA ATCTGCCACT GCTGTAATGG 
     3901 GCATTAATTT CTGTGACTCA ACCCAGGCAG CCAACTTCCA GCTGTGCACC CAGACCCGAC 
     3961 AGTTCTACGT CTCCATTCAG CCACCTGTTG GGGAGCTGAT GGCCCCTGTG TTCATGAGTG 
     4021 AAAATGAGTT TAAGAAGGAA CAGGGAAAGC TGATGGGCAT GAATGAGATC ACAGAGAAAC 
     4081 TCATGCTGCC AGACACCTGT CGGAGTGACC ACATTGTGGT GCAGAAAGTG ACTGCCACTG 
     4141 CCAACCTGGG TCGTGTTCCT TGTGGGACAT CTGATGAGTA CAGGTTTGCA GGGAGGACAC 
     4201 TGACTGGTGG AAGCCTCGTT CTGCTGACCC TGGATGCCCG GCCAGCTGGA GCTGCCCAGC 
     4261 TGACTGTCAA CAGCGAGAAA ATGGTGATTG GCACCATGCT GGTAAAGGAT GTGATACAGG 
     4321 CTCTGACCCA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4381 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4441 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4501 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4561 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4621 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4681 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4741 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4801 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4861 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4921 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4981 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5041 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5101 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5161 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5221 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5281 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5341 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5401 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5461 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5521 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5581 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5641 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5701 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5761 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5821 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5881 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5941 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6001 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6061 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6121 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6181 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6241 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6301 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6361 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6421 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6481 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6541 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6601 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6661 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6721 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6781 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6841 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6901 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6961 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7021 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7081 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7141 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7201 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7261 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7321 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7381 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7441 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7501 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7621 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7681 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7741 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7801 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7861 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7921 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7981 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8041 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8101 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8161 TAACAAAAAT TTAACGCGAA TTTT
//