LOCUS       RC239957                8145 bp    DNA     circular  SYN 
DEFINITION  RGS3 (myc-DDK-tagged) - Human regulator of G-protein signaling 3 (RGS3), transcript variant 10
ACCESSION   NM_001282923
VERSION     NM_001282923.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4302..4393 
                     /label="Myc-DDK"  
     misc_feature    4423..5008 
                     /label="PolyA signal"  
     misc_feature    4480..4499 
                     /label="Restriction Enzyme"  
     misc_feature    5284..5956 
                     /label="ColE1"  
     misc_feature    6386..7180 
                     /label="Kan/Neo"  
     misc_feature    7215..7517 
                     /label="SV40 ori"  
     misc_feature    7603..8080 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4284..4290 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4284 
                     /label="RGS3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCAGGACC GGGTTCTGCT GCTTCACAGT CTCTTTATTC GTGTGGATGG 
     1081 ATATGTCTAT GTGTGTCTCT CTTTCTCGCT GTGTGTGTGT GTATGTTTCC ATTCATCCAC 
     1141 CCCAATGTCT GAATTCTCTT TTAGTTATAG AAGGTAAAGG CCTGATCAGC AAACAGCCTG 
     1201 GCACCTGTGA TCCGTATGTG AAGATTTCTT TGATCCCTGA AGATAGTAGA CTACGCCACC 
     1261 AGAAGACGCA GACCGTTCCA GACTGCAGAG ACCCGGCTTT CCACGAGCAC TTCTTCTTTC 
     1321 CTGTCCAAGA GGAGGATGAT CAGAAGCGTC TCTTGGTTAC TGTGTGGAAC AGGGCCAGCC 
     1381 AGTCCAGACA GAGTGGACTC ATTGGCTGCA TGAGCTTTGG GGTGAAGTCT CTCCTGACTC 
     1441 CAGACAAGGA GATCAGTGGT TGGTACTACC TCCTAGGGGA GCACCTGGGC CGGACCAAGC 
     1501 ACTTGAAGGT GGCCAGGCGG CGACTGCGGC CGCTGAGAGA CCCGCTGCTG AGAATGCCAG 
     1561 GAGGTGGGGA CACTGAGAAT GGGAAGAAAC TAAAGATCAC CATCCCGAGG GGAAAGGACG 
     1621 GCTTTGGCTT CACCATCTGC TGCGACTCTC CAGTTCGAGT CCAGGCCGTG GATTCCGGGG 
     1681 GTCCGGCGGA ACGGGCAGGG CTGCAGCAGC TGGACACGGT GCTGCAGCTG AATGAGAGGC 
     1741 CTGTGGAGCA CTGGAAATGT GTGGAGCTGG CCCACGAGAT CCGGAGCTGC CCCAGTGAGA 
     1801 TCATCCTACT CGTGTGGCGC ATGGTCCCCC AGGTCAAGCC AGGACCAGAT GGCGGGGTCC 
     1861 TGCGGCGGGC CTCCTGCAAG TCGACACATG ACCTCCAGTC ACCCCCCAAC AAACGGGAGA 
     1921 AGAACTGCAC CCATGGGGTC CAGGCACGGC CTGAGCAGCG CCACAGCTGC CACCTGGTAT 
     1981 GTGACAGCTC TGATGGGCTG CTGCTCGGCG GCTGGGAGCG CTACACCGAG GTGGCCAAGC 
     2041 GCGGGGGCCA GCACACCCTG CCTGCACTGT CCCGTGCCAC TGCCCCCACC GACCCCAACT 
     2101 ACATCATCCT GGCCCCGCTG AATCCTGGGA GCCAGCTGCT CCGGCCTGTG TACCAGGAGG 
     2161 ATACCATCCC CGAAGAATCA GGGAGTCCCA GTAAAGGGAA GTCCTACACA GGCCTGGGGA 
     2221 AGAAGTCCCG GCTGATGAAG ACAGTGCAGA CCATGAAGGG CCACGGGAAC TACCAAAACT 
     2281 GCCCGGTTGT GAGGCCGCAT GCCACGCACT CAAGCTATGG CACCTACGTC ACCCTGGCCC 
     2341 CCAAAGTCCT GGTGTTCCCT GTCTTTGTTC AGCCTCTAGA TCTCTGTAAT CCTGCCCGGA 
     2401 CCCTCCTGCT GTCAGAGGAG CTGCTGCTGT ATGAAGGGAG GAACAAGGCT GCCGAGGTGA 
     2461 CACTGTTTGC CTATTCGGAC CTGCTGCTCT TCACCAAGGA GGACGAGCCT GGCCGCTGCG 
     2521 ACGTCCTGAG GAACCCCCTC TACCTCCAGA GTGTGAAGCT GCAGGAAGGT TCTTCAGAAG 
     2581 ACCTGAAATT CTGCGTGCTC TATCTAGCAG AGAAGGCAGA GTGCTTATTC ACTTTGGAAG 
     2641 CGCACTCGCA GGAGCAGAAG AAGAGAGTGT GCTGGTGCCT GTCGGAGAAC ATCGCCAAGC 
     2701 AGCAACAGCT GGCAGCATCA CCCCCGGACA GCAAGATGTT TGAGACGGAG GCAGATGAGA 
     2761 AGAGGGAGAT GGCCTTGGAG GAAGGGAAGG GGCCTGGTGC CGAGGATTCC CCACCCAGCA 
     2821 AGGAGCCCTC TCCTGGCCAG GAGCTTCCTC CAGGACAAGA CCTTCCACCC AACAAGGACT 
     2881 CCCCTTCTGG GCAGGAACCC GCTCCCAGCC AAGAACCACT GTCCAGCAAA GACTCAGCTA 
     2941 CCTCTGAAGG ATCCCCTCCA GGCCCAGATG CTCCGCCCAG CAAGGATGTG CCACCATGCC 
     3001 AGGAACCCCC TCCAGCCCAA GACCTCTCAC CCTGCCAGGA CCTACCTGCT GGTCAAGAAC 
     3061 CCCTGCCTCA CCAGGACCCT CTACTCACCA AAGACCTCCC TGCCATCCAG GAATCCCCCA 
     3121 CCCGGGACCT TCCACCCTGT CAAGATCTGC CTCCTAGCCA GGTCTCCCTG CCAGCCAAGG 
     3181 CCCTTACTGA GGACACCATG AGCTCCGGGG ACCTACTAGC AGCTACTGGG GACCCACCTG 
     3241 CGGCCCCCAG GCCAGCCTTC GTGATCCCTG AGGTCCGGCT GGATAGCACC TACAGCCAGA 
     3301 AGGCAGGGGC AGAGCAGGGC TGCTCGGGAG ATGAGGAGGA TGCAGAAGAG GCCGAGGAGG 
     3361 TGGAGGAGGG GGAGGAAGGG GAGGAGGACG AGGATGAGGA CACCAGCGAT GACAACTACG 
     3421 GAGAGCGCAG TGAGGCCAAG CGCAGCAGCA TGATCGAGAC GGGCCAGGGG GCTGAGGGTG 
     3481 GCCTCTCACT GCGTGTGCAG AACTCGCTGC GGCGCCGGAC GCACAGCGAG GGCAGCCTGC 
     3541 TGCAGGAGCC CCGAGGGCCC TGCTTTGCCT CCGACACCAC CTTGCACTGC TCAGACGGTG 
     3601 AGGGCGCCGC CTCCACCTGG GGCATGCCTT CGCCCAGCAC CCTCAAGAAA GAGCTGGGCC 
     3661 GCAATGGTGG CTCCATGCAC CACCTTTCCC TCTTCTTCAC AGGACACAGG AAGATGAGCG 
     3721 GGGCTGACAC CGTTGGGGAT GATGACGAAG CCTCCCGGAA GAGAAAGAGC AAAAACCTAG 
     3781 CCAAGGACAT GAAGAACAAG CTGGGGATCT TCAGACGGCG GAATGAGTCC CCTGGAGCCC 
     3841 CTCCCGCGGG CAAGGCAGAC AAAATGATGA AGTCATTCAA GCCCACCTCA GAGGAAGCCC 
     3901 TCAAGTGGGG CGAGTCCTTG GAGAAGCTGC TGGTTCACAA ATACGGGTTA GCAGTGTTCC 
     3961 AAGCCTTCCT TCGCACTGAG TTCAGTGAGG AGAATCTGGA GTTCTGGTTG GCTTGTGAGG 
     4021 ACTTCAAGAA GGTCAAGTCA CAGTCCAAGA TGGCATCCAA GGCCAAGAAG ATCTTTGCTG 
     4081 AATACATCGC GATCCAGGCA TGCAAGGAGG TCAACCTGGA CTCCTACACG CGGGAGCACA 
     4141 CCAAGGACAA CCTGCAGAGC GTCACGCGGG GCTGCTTCGA CCTGGCACAG AAGCGCATCT 
     4201 TCGGGCTCAT GGAAAAGGAC TCGTACCCTC GCTTTCTCCG TTCTGACCTC TACCTGGACC 
     4261 TTATTAACCA GAAGAAGATG AGTCCCCCGC TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4321 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4381 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4441 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4501 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4561 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4621 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4681 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4741 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4801 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4861 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4921 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4981 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5041 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5101 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5161 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5221 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5281 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5341 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5401 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5461 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5521 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5581 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5641 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5701 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5761 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5821 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5881 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5941 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6001 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6061 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6121 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6181 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6241 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6301 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6361 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6421 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6481 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6541 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6601 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6661 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6721 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6781 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6841 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6901 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6961 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7021 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7081 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7141 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7201 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7261 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7321 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7381 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7561 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7621 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7681 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7741 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7801 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7861 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7921 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7981 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8041 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8101 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//