LOCUS       RC239954                8139 bp    DNA     circular  SYN 
DEFINITION  ANLN (myc-DDK-tagged) - Human anillin, actin binding protein (ANLN), transcript variant 3
ACCESSION   NM_001284302
VERSION     NM_001284302.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4296..4387 
                     /label="Myc-DDK"  
     misc_feature    4417..5002 
                     /label="PolyA signal"  
     misc_feature    4474..4493 
                     /label="Restriction Enzyme"  
     misc_feature    5278..5950 
                     /label="ColE1"  
     misc_feature    6380..7174 
                     /label="Kan/Neo"  
     misc_feature    7209..7511 
                     /label="SV40 ori"  
     misc_feature    7597..8074 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4278..4284 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4278 
                     /label="ANLN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCGTTT ACGGAGAAAC TGCTGGAGCG AACCCGTGCC AGGCGAGAGA 
     1081 ATCTTCAGAG AAAAATGGCT GAGAGGCCCA CAGCAGCTCC AAGGTCTATG ACTCATGCTA 
     1141 AGCGAGCTAG ACAGCCACTT TCAGAAGCAA GTAACCAGCA GCCCCTCTCT GGTGGTGAAG 
     1201 AGAAATCTTG TACAAAACCA TCGCCATCAA AAAAACGCTG TTCTGACAAC ACTGAAGTAG 
     1261 AAGTTTCTAA CTTGGAAAAT AAACAACCAG TTGAGTCGAC ATCTGCAAAA TCTTGTTCTC 
     1321 CAAGTCCTGT GTCTCCTCAG GTGCAGCCAC AAGCAGCAGA TACCATCAGT GATTCTGTTG 
     1381 CTGTCCCGGC ATCACTGCTG GGCATGAGGA GAGGGCTGAA CTCAAGATTG GAAGCAACTG 
     1441 CAGCCTCCTC AGTTAAAACA CGTATGCAAA AACTTGCAGA GCAACGGCGC CGTTGGGATA 
     1501 ATGATGATAT GACAGATGAC ATTCCTGAAA GCTCACTCTT CTCACCAATG CCATCAGAGG 
     1561 AAAAGGCTGC TTCCCCTCCC AGACCTCTGC TTTCAAATGC CTCGGCAACT CCAGTTGGCA 
     1621 GAAGGGGCCG TCTGGCCAAT CTTGCTGCAA CTATTTGCTC CTGGGAAGAT GATGTAAATC 
     1681 ACTCATTTGC AAAACAAAAC AGTGTACAAG AACAGCCTGG TACCGCTTGT TTATCCAAAT 
     1741 TTTCCTCTGC AAGTGGAGCA TCTGCTAGGA TCAATAGCAG CAGTGTTAAG CAGGAAGCTA 
     1801 CATTCTGTTC CCAAAGGGAT GGCGATGCCT CTTTGAATAA AGCCCTATCC TCAAGTGCTG 
     1861 ATGATGCGTC TTTGGTTAAT GCCTCAATTT CCAGCTCTGT GAAAGCTACT TCTCCAGTGA 
     1921 AATCTACTAC ATCTATCACT GATGCTAAAA GTTGTGAGGG ACAAAATCCT GAGCTACTTC 
     1981 CAAAAACTCC TATTAGTCCT CTGAAAACGG GGGTATCGAA ACCAATTGTG AAGTCAACTT 
     2041 TATCCCAGAC AGTTCCATCC AAGGGAGAAT TAAGTAGAGA AATTTGTCTG CAATCTCAAT 
     2101 CTAAAGACAA ATCTACGACA CCAGGAGGAA CAGGAATTAA GCCTTTCCTG GAACGCTTTG 
     2161 GAGAGCGTTG TCAAGAACAT AGCAAAGAAA GTCCAGCTCG TAGCACACCC CACAGAACCC 
     2221 CCATTATTAC TCCAAATACA AAGGCCATCC AAGAAAGATT ATTCAAGCAA GACACATCTT 
     2281 CATCTACTAC CCATTTAGCA CAACAGCTCA AGCAGGAACG TCAAAAAGAA CTAGCATGTC 
     2341 TTCGTGGCCG ATTTGACAAG GGCAATATAT GGAGTGCAGA AAAAGGCGGA AACTCAAAAA 
     2401 GCAAACAACT AGAAACCAAA CAGGAAACTC ACTGTCAGAG CACTCCCCTC AAAAAACACC 
     2461 AAGGTGTTTC AAAAACTCAG TCACTTCCAG TAACAGAAAA GGTGACCGAA AACCAGATAC 
     2521 CAGCCAAAAA TTCTAGTACA GAACCTAAAG TGATACGTGA AATTGAGATG AGTGTGGATG 
     2581 ATGATGATAT CAATAGTTCG AAAGTAATTA ATGACCTCTT CAGTGATGTC CTAGAGGAAG 
     2641 GTGAACTAGA TATGGAGAAG AGCCAAGAGG AGATGGATCA AGCATTAGCA GAAAGCAGCG 
     2701 AAGAACAGGA AGATGCACTG AATATCTCCT CAATGTCTTT ACTTGCACCA TTGGCACAAA 
     2761 CAGTTGGTGT GGTAAGTCCA GAGAGTTTAG TGTCCACACC TAGACTGGAA TTGAAAGACA 
     2821 CCAGCAGAAG TGATGAAAGT CCAAAACCAG GAAAATTCCA AAGAACTCGT GTCCCTCGAG 
     2881 CTGAATCTGG TGATAGCCTT GGTTCTGAAG ATCGTGATCT TCTTTACAGC ATTGATGCAT 
     2941 ATAGATCTCA AAGATTCAAA GAAACAGAAC GTCCATCAAT AAAGCAGGTG ATTGTTCGGA 
     3001 AGGAAGATGT TACTTCAAAA CTGGATGAAA AAAATAATGC CTTTCCTTGT CAAGTTAATA 
     3061 TCAAACAGAA AATGCAGGAA CTCAATAACG AAATAAATAT GCAACAGACA GTGATCTATC 
     3121 AAGCTAGCCA GGCTCTTAAC TGCTGTGTTG ATGAAGAACA TGGAAAAGGG TCCCTAGAAG 
     3181 AAGCTGAAGC AGAAAGACTT CTTCTAATTG CAACTGGGAA GAGAACACTT TTGATTGATG 
     3241 AATTGAATAA ATTGAAGAAC GAAGGACCTC AGAGGAAGAA TAAGGCTAGT CCCCAAAGTG 
     3301 AATTTATGCC ATCCAAAGGA TCAGTTACTT TGTCAGAAAT CCGCTTGCCT CTAAAAGCAG 
     3361 ATTTTGTCTG CAGTACGGTT CAGAAACCAG ATGCAGCAAA TTACTATTAC TTAATTATAC 
     3421 TAAAAGCAGG AGCTGAAAAT ATGGTAGCCA CACCATTAGC AAGTACTTCA AACTCTCTTA 
     3481 ACGGTGATGC TCTGACATTC ACTACTACAT TTACTCTGCA AGATGTATCC AATGACTTTG 
     3541 AAATAAATAT TGAAGTTTAC AGCTTGGTGC AAAAGAAAGA TCCCTCAGGC CTTGATAAGA 
     3601 AGAAAAAAAC ATCCAAGTCC AAGGCTATTA CTCCAAAGCG ACTCCTCACA TCTATAACCA 
     3661 CAAAAAGCAA CATTCATTCT TCAGTCATGG CCAGTCCAGG AGGTCTTAGT GCTGTGCGAA 
     3721 CCAGCAACTT CGCCCTTGTT GGATCTTACA CATTATCATT GTCTTCAGTA GGAAATACTA 
     3781 AGTTTGTTCT GGACAAGGTC CCCTTTTTAT CTTCTTTGGA AGGTCATATT TATTTAAAAA 
     3841 TAAAATGTCA AGTGAATTCC AGTGTTGAAG AAAGAGGTTT TCTAACCATA TTTGAAGATG 
     3901 TTAGTGGTTT TGGTGCCTGG CATCGAAGAT GGTGTGTTCT TTCTGGAAAC TGTATATCTT 
     3961 ATTGGACTTA TCCAGATGAT GAGAAACGCA AGAATCCCAT AGGAAGGATA AATCTGGCTA 
     4021 ATTGTACCAG TCGTCAGATA GAACCAGCCA ACAGAGAATT TTGTGCAAGA CGCAACACTT 
     4081 TTGAATTAAT TACTGTCCGA CCACAAAGAG AAGATGACCG AGAGACTCTT GTCAGCCAAT 
     4141 GCAGGGACAC ACTCTGTGTT ACCAAGAACT GGCTGTCTGC AGATACTAAA GAAGAGCGGG 
     4201 ATCTCTGGAT GCAAAAACTC AATCAAGTTC TTGTTGATAT TCGCCTCTGG CAACCTGATG 
     4261 CTTGCTACAA ACCTATTGGA AAGCCTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4321 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4381 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4441 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4501 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4561 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4621 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4681 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4741 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4801 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4861 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4921 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4981 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5041 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5101 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5161 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5221 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5281 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5341 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5401 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5461 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5521 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5581 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5641 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5701 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5761 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5821 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5881 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5941 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6001 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6061 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6121 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6181 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6241 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6301 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6361 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6421 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6481 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6541 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6601 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6661 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6721 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6781 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6841 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6901 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6961 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7021 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7081 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7141 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7201 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7261 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7321 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7381 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7441 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7501 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7561 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7621 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7681 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7741 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7801 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7861 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7921 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7981 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8041 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8101 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//