LOCUS       RC239946                8124 bp    DNA     circular  SYN 
DEFINITION  TEK (myc-DDK-tagged) - Human TEK tyrosine kinase, endothelial (TEK), transcript variant 2
ACCESSION   NM_001290077
VERSION     NM_001290077.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4281..4372 
                     /label="Myc-DDK"  
     misc_feature    4402..4987 
                     /label="PolyA signal"  
     misc_feature    4459..4478 
                     /label="Restriction Enzyme"  
     misc_feature    5263..5935 
                     /label="ColE1"  
     misc_feature    6365..7159 
                     /label="Kan/Neo"  
     misc_feature    7194..7496 
                     /label="SV40 ori"  
     misc_feature    7582..8059 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4263..4269 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4263 
                     /label="TEK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTTTA GCCAGCTTAG TTCTCTGTGG AGTCAGCTTG CTCCTTTCTG 
     1081 GAACTGTGGA AGGTGCCATG GACTTGATCT TGATCAATTC CCTACCTCTT GTATCTGATG 
     1141 CTGAAACATC TCTCACCTGC ATTGCCTCTG GGTGGCGCCC CCATGAGCCC ATCACCATAG 
     1201 GAAGGGACTT TGAAGCCTTA ATGAACCAGC ACCAGGATCC GCTGGAAGTT ACTCAAGATG 
     1261 TGACCAGAGA ATGGGCTAAA AAAGTTGTTT GGAAGAGAGA AAAGGCTAGT AAGATCAATG 
     1321 GTGCTTATTT CTGTGAAGGG CGAGTTCGAG GAGAGGCAAT CAGGATACGA ACCATGAAGA 
     1381 TGCGTCAACA AGCTTCCTTC CTACCAGCTA CTTTAACTAT GACTGTGGAC AAGGGAGATA 
     1441 ACGTGAACAT ATCTTTCAAA AAGGTATTGA TTAAAGAAGA AGATGCAGTG ATTTACAAAA 
     1501 ATGGTTCCTT CATCCATTCA GTGCCCCGGC ATGAAGTACC TGATATTCTA GAAGTACACC 
     1561 TGCCTCATGC TCAGCCCCAG GATGCTGGAG TGTACTCGGC CAGGTATATA GGAGGAAACC 
     1621 TCTTCACCTC GGCCTTCACC AGGCTGATAG TCCGGAGATG TGAAGCCCAG AAGTGGGGAC 
     1681 CTGAATGCAA CCATCTCTGT ACTGCTTGTA TGAACAATGG TGTCTGCCAT GAAGATACTG 
     1741 GAGAATGCAT TTGCCCTCCT GGGTTTATGG GAAGGACGTG TGAGAAGGCT TGTGAACTGC 
     1801 ACACGTTTGG CAGAACTTGT AAAGAAAGGT GCAGTGGACA AGAGGGATGC AAGTCTTATG 
     1861 TGTTCTGTCT CCCTGACCCC TATGGGTGTT CCTGTGCCAC AGGCTGGAAG GGTCTGCAGT 
     1921 GCAATGAAGG CATACCGAGG ATGACCCCAA AGATAGTGGA TTTGCCAGAT CATATAGAAG 
     1981 TAAACAGTGG TAAATTTAAT CCCATTTGCA AAGCTTCTGG CTGGCCGCTA CCTACTAATG 
     2041 AAGAAATGAC CCTGGTGAAG CCGGATGGGA CAGTGCTCCA TCCAAAAGAC TTTAACCATA 
     2101 CGGATCATTT CTCAGTAGCC ATATTCACCA TCCACCGGAT CCTCCCCCCT GACTCAGGAG 
     2161 TTTGGGTCTG CAGTGTGAAC ACAGTGGCTG GGATGGTGGA AAAGCCCTTC AACATTTCTG 
     2221 TTAAAGTTCT TCCAAAGCCC CTGAATGCCC CAAACGTGAT TGACACTGGA CATAACTTTG 
     2281 CTGTCATCAA CATCAGCTCT GAGCCTTACT TTGGGGATGG ACCAATCAAA TCCAAGAAGC 
     2341 TTCTATACAA ACCCGTTAAT CACTATGAGG CTTGGCAACA TATTCAAGTG ACAAATGAGA 
     2401 TTGTTACACT CAACTATTTG GAACCTCGGA CAGAATATGA ACTCTGTGTG CAACTGGTCC 
     2461 GTCGTGGAGA GGGTGGGGAA GGGCATCCTG GACCTGTGAG ACGCTTCACA ACAGCTTCTA 
     2521 TCGGACTCCC TCCTCCAAGA GGTCTAAATC TCCTGCCTAA AAGTCAGACC ACTCTAAATT 
     2581 TGACCTGGCA ACCAATATTT CCAAGCTCGG AAGATGACTT TTATGTTGAA GTGGAGAGAA 
     2641 GGTCTGTGCA AAAAAGTGAT CAGCAGAATA TTAAAGTTCC AGGCAACTTG ACTTCGGTGC 
     2701 TACTTAACAA CTTACATCCC AGGGAGCAGT ACGTGGTCCG AGCTAGAGTC AACACCAAGG 
     2761 CCCAGGGGGA ATGGAGTGAA GATCTCACTG CTTGGACCCT TAGTGACATT CTTCCTCCTC 
     2821 AACCAGAAAA CATCAAGATT TCCAACATTA CACACTCCTC AGCTGTGATT TCTTGGACAA 
     2881 TATTGGATGG CTATTCTATT TCTTCTATTA CTATCCGTTA CAAGGTTCAA GGCAAGAATG 
     2941 AAGACCAGCA CGTTGATGTG AAGATAAAGA ATGCCACCAT CACTCAGTAT CAGCTCAAGG 
     3001 GCCTAGAGCC TGAAACAGCA TACCAGGTGG ACATTTTTGC AGAGAACAAC ATAGGGTCAA 
     3061 GCAACCCAGC CTTTTCTCAT GAACTGGTGA CCCTCCCAGA ATCTCAAGCA CCAGCGGACC 
     3121 TCGGAGGGGG GAAGATGCTG CTTATAGCCA TCCTTGGCTC TGCTGGAATG ACCTGCCTGA 
     3181 CTGTGCTGTT GGCCTTTCTG ATCATATTGC AATTGAAGAG GGCAAATGTG CAAAGGAGAA 
     3241 TGGCCCAAGC CTTCCAAAAC GTGAGGGAAG AACCAGCTGT GCAGTTCAAC TCAGGGACTC 
     3301 TGGCCCTAAA CAGGAAGGTC AAAAACAACC CAGATCCTAC AATTTATCCA GTGCTTGACT 
     3361 GGAATGACAT CAAATTTCAA GATGTGATTG GGGAGGGCAA TTTTGGCCAA GTTCTTAAGG 
     3421 CGCGCATCAA GAAGGATGGG TTACGGATGG ATGCTGCCAT CAAAAGAATG AAAGAATATG 
     3481 CCTCCAAAGA TGATCACAGG GACTTTGCAG GAGAACTGGA AGTTCTTTGT AAACTTGGAC 
     3541 ACCATCCAAA CATCATCAAT CTCTTAGGAG CATGTGAACA TCGAGGCTAC TTGTACCTGG 
     3601 CCATTGAGTA CGCGCCCCAT GGAAACCTTC TGGACTTCCT TCGCAAGAGC CGTGTGCTGG 
     3661 AGACGGACCC AGCATTTGCC ATTGCCAATA GCACCGCGTC CACACTGTCC TCCCAGCAGC 
     3721 TCCTTCACTT CGCTGCCGAC GTGGCCCGGG GCATGGACTA CTTGAGCCAA AAACAGTTTA 
     3781 TCCACAGGGA TCTGGCTGCC AGAAACATTT TAGTTGGTGA AAACTATGTG GCAAAAATAG 
     3841 CAGATTTTGG ATTGTCCCGA GGTCAAGAGG TGTATGTGAA AAAGACAATG GGAAGGCTCC 
     3901 CAGTGCGCTG GATGGCCATC GAGTCACTGA ATTACAGTGT GTACACAACC AACAGTGATG 
     3961 TATGGTCCTA TGGTGTGTTA CTATGGGAGA TTGTTAGCTT AGGAGGCACA CCCTACTGCG 
     4021 GGATGACTTG TGCAGAACTC TACGAGAAGC TGCCCCAGGG CTACAGACTG GAGAAGCCCC 
     4081 TGAACTGTGA TGATGAGGTG TATGATCTAA TGAGACAATG CTGGCGGGAG AAGCCTTATG 
     4141 AGAGGCCATC ATTTGCCCAG ATATTGGTGT CCTTAAACAG AATGTTAGAG GAGCGAAAGA 
     4201 CCTACGTGAA TACCACGCTT TATGAGAAGT TTACTTATGC AGGAATTGAC TGTTCTGCTG 
     4261 AAGAAGCGGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4321 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4381 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4441 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4501 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4561 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4621 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4681 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4741 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4801 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4861 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4921 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4981 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5041 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5101 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5161 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5221 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5281 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5341 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5401 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5461 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5521 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5581 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5641 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5701 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5761 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5821 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5881 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5941 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6001 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6061 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6121 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6181 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6241 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6301 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6361 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6421 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6481 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6541 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6601 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6661 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6721 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6781 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6841 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6901 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6961 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7021 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7081 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7141 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7201 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7261 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7321 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7561 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7621 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7681 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7741 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7801 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7861 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7921 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7981 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8041 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8101 TAACAAAAAT TTAACGCGAA TTTT
//