LOCUS       RC239939                8124 bp    DNA     circular  SYN 
DEFINITION  PEX1 (myc-DDK-tagged) - Human peroxisomal biogenesis factor 1 (PEX1), transcript variant 3
ACCESSION   NM_001282678
VERSION     NM_001282678.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4279..4370 
                     /label="Myc-DDK"  
     misc_feature    4400..4985 
                     /label="PolyA signal"  
     misc_feature    4457..4476 
                     /label="Restriction Enzyme"  
     misc_feature    5261..5933 
                     /label="ColE1"  
     misc_feature    6363..7157 
                     /label="Kan/Neo"  
     misc_feature    7192..7494 
                     /label="SV40 ori"  
     misc_feature    7580..8057 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4254..4261 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4254 
                     /label="PEX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG ATGAAAGAAC TTCAAACCAA GCAACTTCAG TCAAATACTG 
     1081 TGGGAATCAC TGAATCTAAT GAAAACGAGT CAGAGATTCC AGTTGACTCA TCATCAGTAG 
     1141 CAAGTTTATG GACTATGATA GGAAGCATTT TTTCCTTTCA ATCTGAGAAG AAACAAGAGA 
     1201 CATCTTGGGG TTTAACTGAA ATCAATGCAT TCAAAAATAT GCAGTCAAAG GTTGTTCCTC 
     1261 TAGACAATAT TTTCAGAGTA TGCAAATCTC AACCTCCTAG TATATATAAC GCGTCAGCAA 
     1321 CCTCTGTTTT TCATAAACAC TGTGCCATTC ATGTATTTCC ATGGGACCAG GAATATTTTG 
     1381 ATGTAGAGCC CAGCTTTACT GTGACATATG GAAAGCTAGT TAAGCTACTT TCTCCAAAGC 
     1441 AACAGCAAAG TAAAACAAAA CAAAATGTGT TATCACCTGA AAAAGAGAAG CAGATGTCAG 
     1501 AGCCACTAGA TCAAAAAAAA ATTAGGTCAG ATCATAATGA AGAAGATGAG AAGGCCTGTG 
     1561 TGCTACAAGT AGTCTGGAAT GGACTTGAAG AATTGAACAA TGCCATCAAA TATACCAAAA 
     1621 ATGTAGAAGT TCTCCATCTT GGGAAAGTCT GGATTCCAGA TGACCTGAGG AAGAGACTAA 
     1681 ATATAGAAAT GCATGCCGTA GTCAGGATAA CTCCAGTGGA AGTTACCCCT AAAATTCCAA 
     1741 GATCTCTAAA GTTACAACCT AGAGAGAATT TACCTAAAGA CATAAGTGAA GAAGACATAA 
     1801 AAACTGTATT TTATTCATGG CTACAGCAGT CTACTACCAC CATGCTTCCT TTGGTAATAT 
     1861 CAGAGGAAGA ATTTATTAAG CTGGAAACTA AAGATGGACT GAAGGAATTT TCTCTGAGTA 
     1921 TAGTTCATTC TTGGGAAAAA GAAAAAGATA AAAATATTTT TCTGTTGAGT CCCAATTTGC 
     1981 TGCAGAAGAC TACAATACAA GTCCTTCTAG ATCCTATGGT AAAAGAAGAA AACAGTGAGG 
     2041 AAATTGACTT TATTCTTCCT TTTTTAAAGC TGAGCTCTTT GGGAGGAGTG AATTCCTTAG 
     2101 GCGTATCCTC CTTGGAGCAC ATCACTCACA GCCTCCTGGG ACGCCCTTTG TCTCGGCAGC 
     2161 TGATGTCTCT TGTTGCAGGA CTTAGGAATG GAGCTCTTTT ACTCACAGGA GGAAAGGGAA 
     2221 GTGGAAAATC AACTTTAGCC AAAGCAATCT GTAAAGAAGC ATTTGACAAA CTGGATGCCC 
     2281 ATGTGGAGAG AGTTGACTGT AAAGCTTTAC GAGGAAAAAG GCTTGAAAAC ATACAAAAAA 
     2341 CCCTAGAGGT GGCTTTCTCA GAGGCAGTGT GGATGCAGCC ATCTGTTGTC CTGCTGGATG 
     2401 ACCTTGACCT CATTGCTGGA CTGCCTGCTG TCCCGGAACA TGAGCACAGT CCTGATGCGG 
     2461 TGCAGAGCCA GCGGCTTGCT CATGCTTTGA ATGATATGAT AAAAGAGTTT ATCTCCATGG 
     2521 GAAGTTTGGT TGCACTGATT GCCACAAGTC AGTCTCAGCA ATCTCTACAT CCTTTACTTG 
     2581 TTTCTGCTCA AGGAGTTCAC ATATTTCAGT GCGTCCAACA CATTCAGCCT CCTAATCAGG 
     2641 AACAAAGATG TGAAATTCTG TGTAATGTAA TAAAAAATAA ATTGGACTGT GATATAAACA 
     2701 AGTTCACCGA TCTTGACCTG CAGCATGTAG CTAAAGAAAC TGGCGGGTTT GTGGCTAGAG 
     2761 ATTTTACAGT ACTTGTGGAT CGAGCCATAC ATTCTCGACT CTCTCGTCAG AGTATATCCA 
     2821 CCAGAGAAAA ATTAGTTTTA ACAACATTGG ACTTCCAAAA GGCTCTCCGC GGATTTCTTC 
     2881 CTGCGTCTTT GCGAAGTGTC AACCTGCATA AACCTAGAGA CCTGGGTTGG GACAAGATTG 
     2941 GTGGGTTACA TGAAGTTAGG CAGATACTCA TGGATACTAT CCAGTTACCT GCCAAGTATC 
     3001 CAGAATTATT TGCAAACTTG CCCATACGAC AAAGAACAGG AATACTGTTG TATGGTCCGC 
     3061 CTGGAACAGG AAAAACCTTA CTAGCTGGGG TAATTGCACG AGAGAGTAGA ATGAATTTTA 
     3121 TAAGTGTCAA GGGGCCAGAG TTACTCAGCA AATACATTGG AGCAAGTGAA CAAGCTGTTC 
     3181 GGGATATTTT TATTAGAGCA CAGGCTGCAA AGCCCTGCAT TCTTTTCTTT GATGAATTTG 
     3241 AATCCATTGC TCCTCGGCGG GGTCATGATA ATACAGGAGT TACAGACCGA GTAGTTAACC 
     3301 AGTTGCTGAC TCAGTTGGAT GGAGTAGAAG GCTTACAGGG TGTTTATGTA TTGGCTGCTA 
     3361 CTAGTCGCCC TGACTTGATT GACCCTGCCC TGCTTAGGCC TGGTCGACTA GATAAATGTG 
     3421 TATACTGTCC TCCTCCTGAT CAGGTGTCAC GTCTTGAAAT TTTAAATGTC CTCAGTGACT 
     3481 CTCTACCTCT GGCAGATGAT GTTGACCTTC AGCATGTAGC ATCAGTAACT GACTCCTTTA 
     3541 CTGGAGCTGA TCTGAAAGCT TTACTTTACA ATGCCCAATT GGAGGCCTTA CATGGAATGC 
     3601 TGCTCTCGAG TGGACTCCAG GATGGAAGTT CCAGCTCTGA TAGTGACCTA AGTCTGTCTT 
     3661 CAATGGTCTT TCTTAACCAT AGCAGTGGCT CTGACGATTC AGCTGGAGAT GGAGAATGTG 
     3721 GCTTAGATCA GTCCCTTGTT TCTTTAGAGA TGTCCGAGAT CCTTCCAGAT GAATCAAAAT 
     3781 TCAATATGTA CCGGCTCTAC TTTGGAAGCT CTTATGAATC AGAACTTGGA AATGGAACCT 
     3841 CTTCTGATTT GAGCTCACAA TGTCTCTCTG CACCAAGCTC CATGACTCAG GATTTGCCTG 
     3901 GAGTTCCTGG GAAAGACCAG TTGTTTTCAC AGCCTCCAGT GTTAAGGACA GCTTCACAAG 
     3961 AGGGTTGCCA AGAACTTACA CAAGAACAAA GAGATCAACT GAGGGCAGAT ATCAGTATTA 
     4021 TCAAAGGCAG ATACCGGAGC CAAAGTGGAG AGGACGAATC CATGAACCAA CCAGGACCAA 
     4081 TCAAAACCAG ACTGGCTATT AGTCAGTCAC ATTTAATGAC TGCACTTGGT CACACAAGAC 
     4141 CATCCATTAG TGAAGATGAC TGGAAGAATT TTGCTGAGCT ATATGAAAGC TTTCAAAATC 
     4201 CAAAGAGGAG AAAAAATCAA AGTGGAACAA TGTTTCGACC TGGACAGAAA GTAACTTTAG 
     4261 CAAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4321 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4381 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4441 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4501 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4561 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4621 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4681 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4741 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4801 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4861 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4921 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4981 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5041 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5101 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5161 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5221 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5281 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5341 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5401 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5461 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5521 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5581 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5641 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5701 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5761 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5821 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5881 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5941 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6001 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6061 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6121 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6181 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6241 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6301 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6361 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6421 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6481 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6541 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6601 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6661 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6721 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6781 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6841 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6901 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6961 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7021 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7081 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7141 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7201 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7261 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7321 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7561 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7621 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7681 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7741 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7801 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7861 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7921 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7981 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8041 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8101 TAACAAAAAT TTAACGCGAA TTTT
//