LOCUS       RC239929                8073 bp    DNA     circular  SYN 
DEFINITION  KRBA1 (myc-DDK-tagged) - Human KRAB-A domain containing 1 (KRBA1), transcript variant 2
ACCESSION   NM_001290187
VERSION     NM_001290187.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4230..4321 
                     /label="Myc-DDK"  
     misc_feature    4351..4936 
                     /label="PolyA signal"  
     misc_feature    4408..4427 
                     /label="Restriction Enzyme"  
     misc_feature    5212..5884 
                     /label="ColE1"  
     misc_feature    6314..7108 
                     /label="Kan/Neo"  
     misc_feature    7143..7445 
                     /label="SV40 ori"  
     misc_feature    7531..8008 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4212..4218 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4212 
                     /label="KRBA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCGCCAG GTGTCCATCA CCTTCAAGGA CTTGGCCGTG CGGTTCTCGG 
     1081 AGGAGGAGTG GCGGCTCCTG GAGGAGGGGC AGAGGGAGTT CTACCGAGAC GTGATGCGGG 
     1141 AGAACTACGA GACGCTGGTC TCTGTGGGGA CAGCTGAGCT GCTCCCCCTC TCTGCTTTCC 
     1201 TGTCACCCTC AGAGCCTGGA AGAGCTGTTG GGGGAGGGAG CCACGCTGAT GAGGGGCAGG 
     1261 AGCCTGCTGG TTGTGGAGAT CCCCAGGGGG GACAGCCCCG GCACAGCCTG CACCTCACAG 
     1321 CCCTGGTGCA GCTGGTGAAG GAGATCCCAG AGTTCTTGTT TGGAGAAGTC AAGGGCGCTA 
     1381 TGGACAGCCC CGAGAGCGAG AGCCGGGGAG CCAGCCTGGA TGGAGAGAGA GCGAGCCCCG 
     1441 AGGCTGCGGC AAGAGAGCCC TGCCCTCTCC GAGGCCTGCT CAGCTGCCTT CCAGACGGCC 
     1501 CTACCAGCCA GCCCCACCTG GCCACCACGC CCACCGACAG CTCGTGTTCC AGTGGCCCAA 
     1561 CTGGTGACGG GGTCCAGGGA AGTCCTCTCC CCATCAAAAC TGCCGACAAA CCGTGGCCTA 
     1621 CAAGGAAGGA AGGCCCAGGA GCCCTGGGCG GGGAGCCCAG CCCTCCCACC CATAGCCCCA 
     1681 GCAGGAGGAA GAGCCACAGA GGACAGGAGA GAGGGACCTC AGAGGCCGGA ATTTCTCCTG 
     1741 GGAACAGCCC CTTGCAAGGC CTCATCAACT GTCTGAAGGA AATCCTTGTG CCTGGGCCCC 
     1801 GGCACCCCGA GACATCCCCA AGCTTCTTGC CACCTCTCCC TAGCCTGGGC ACGTCCAGGC 
     1861 TAACCAGAGC AGACCTGGGG CCTGGGAGCC CGCCCTGGGC AGTGAAGACC GAGGCGGTTT 
     1921 CAGGGGATTG TCCCCTCCAG GGTCTGCTAC ACTGTCTGAA GGAGCTCCCC GAGGCCCAGG 
     1981 ACAGGCATCC CAGTCCCTCA GGAGTGGGGA ACCGACGGCT ACAGGAGAAT CCAGGAGCCT 
     2041 GGAAAAGGGG TTCTGGAGGG CCTGGATACC TCCTGACCCC TCCTCCCCAT CCTGATCTTG 
     2101 GAGCTGGCGG TCTGCTCTCT GTGAAGATGG AGAACAGCTG GGTCCAGAGC CCCCCAGGAC 
     2161 CTGCATCCTG TCAGCCTGGC AGGCAGCCCC TCAGTCCCTC AGCCACTGGA GACACCAGAG 
     2221 GGGTCCCCCA ACCCAGCTGG GGCCCTGAGG CTCAAGCTGC CAGTGCCTCA AGCTCACCGC 
     2281 TGGAAGCCCT GGAAGCCTGT CTGAAGGGCA TTCCCCCAAA TGGGTCGTCA CCTTCCCAGC 
     2341 TGCCACCCAC TTCTTGTTCC CAGAACCCCC AGCCAGGAGA CTCTAGGTCT CAGAAGCCTG 
     2401 AACTGCAACC CCACAGATCA CACAGTGAAG AAGCGACCAG AGAGCCTGTT CTGCCTCTGG 
     2461 GTCTGCAGAG CTGTGTGAGA GATGGCCCCA GCAGGCCCCT GGCCCCCCGA GGAACCCCCA 
     2521 CCAGCTTCTC CTCATCCAGC AGCACCGACT GGGACCTGGA TTTTGGGAGT CCTGTGGGGA 
     2581 ACCAGGGGCA GCATCCTGGA AAAGGAAGCC CACCAGGAAG CTCCCCACTG CAGGGTCTGG 
     2641 AGAATTGTCT CAAGGAGATA CCTGTGCCTG TGCTGCGGCC TGCCTGGCCC TGCTCCTCAG 
     2701 CAGCAGACAG GGGACCGAGG AGAGCAGAGC CCAGGAACTG GACAGCAGAC AAGGAAGGAC 
     2761 TGAGGGCTGA GGCCTGCGAG TCAGCCCGTC TCGGGCAGGG TAGGGGAGAA GCGCCCACCC 
     2821 GGAGCCTCCA TCTGGTCAGC CCACAGGTGT TCACCTCCAG CTGCGTCCCC GCCTGCCACC 
     2881 AGCGGGGGTT CAAAGACCCT GGGGCCACCA GGCCAGGAGT GTGGAGGTGG CTCCCAGAGG 
     2941 GGTCTGCCCC CAAGCCCTCC CCGCTGCACT GCCTGGAGAG CGCCCTGAGG GGGATCTTGC 
     3001 CTGTAAGGCC CTTGCGCTTC GCCTGCGTGG GAGGCCCCAG CCCCAGCCCC AGCCCCGGCT 
     3061 CCAGCTCGAG CTTCAGCGGC TCTGAAGGAG AAGACCCGAG GCCAGAGCCT GATCTCTGGA 
     3121 AGCCGCTCCC CCAGGAGAGG GACCGCCTTC CCAGCTGTAA GCCTCCTGTC CCTCTGTCCC 
     3181 CATGTCCTGG TGGGACCCCT GCTGGCAGCA GTGGCGGCAG CCCTGGTGAA GACCCCAGGA 
     3241 GAACAGAGCC CAGGTACTGC AGCGGCCTCG GTGCAGGTAC AGCTCAGGAT CCCTGCCCGG 
     3301 TTTCTCAGCT GGAGAAAAGG CCCAGGGTTA GTGAAGCATC CAGAGGCCTG GAGCTTGGAC 
     3361 ATGGAAGACC CAGAGTTGCA GCCAAGACCC ATGAGAGGCT GCTCCCCCAG GGCCCGCCTG 
     3421 AGCTGCCCAG TGAGTCTCCC CCTCCGGAGC TGCCCCCTCC GGAAGCTGCG CCTCCTGTGT 
     3481 TGCCAGCCTC CTCCCTGCAG CCGCCATGCC ACTGTGGGAA GCCCCTGCAG CAGGAGCTGC 
     3541 ACAGCCTCGG TGCTGCCCTT GCGGAGAAGC TGGATCGGCT CGCCACAGCG CTGGCAGGCC 
     3601 TGGCTCAGGA AGTGGCCACC ATGAGGACCC AGGTGAATCG GCTGGGGAGG CGCCCCCAAG 
     3661 GCCCTGGGCC AATGGGCCAA GCTTCCTGGA TGTGGACCCT CCCACGGGGA CCTCGCTGGG 
     3721 CTCATGGCCC TGGTCACAGA CATCTGCCCT ACTGGAGGCA GAAGGGACCC ACGAGGCCTA 
     3781 AACCAAAGAT CCTGCGTGGC CAGGGAGAGA GCTGCAGGGC TGGTGACCTG CAAGGACTCT 
     3841 CCAGAGGGAC CGCTCGCCGG GCACGTCCGC TGCCTCCAGA CGCTCCCCCG GCAGAACCTC 
     3901 CTGGGCTCCA CTGCAGCTCT TCCCAGCAGC TGCTGTCCTC TACACCCAGC TGCCATGCTG 
     3961 CGCCGCCTGC ACACCCCCTC CTCGCACATA CCGGGGGCCA CCAGAGCCCC CTTCCCCCTT 
     4021 TAGTGCCTGC TGCCTTACCC CTGCAGGGAG CCTCTCCTCC TGCAGCCAGT GCAGATGCAG 
     4081 ACGTGCCGAC CTCAGGAGTG GCACCAGACG GGATCCCAGA GCGGCCCAAG GAGCCGAGCA 
     4141 GCCTGCTGGG AGGAGTGCAG AGGGCCCTCC AGGAAGAACT GTGGGGTGGG GAGCACAGGG 
     4201 ACCCGAGATG GGGGGCGCAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4261 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5881 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5941 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6001 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6061 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6121 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6181 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6241 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6301 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6361 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6421 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6481 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6541 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6601 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6661 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6721 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6781 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6841 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6901 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6961 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7021 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7081 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7141 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7201 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7261 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7321 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7501 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7561 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7621 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7681 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7741 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7801 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7861 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7921 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7981 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8041 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//