LOCUS       RC239924                8055 bp    DNA     circular  SYN 
DEFINITION  MSH6 (myc-DDK-tagged) - Human mutS homolog 6 (MSH6), transcript variant 3
ACCESSION   NM_001281493
VERSION     NM_001281493.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4212..4303 
                     /label="Myc-DDK"  
     misc_feature    4333..4918 
                     /label="PolyA signal"  
     misc_feature    4390..4409 
                     /label="Restriction Enzyme"  
     misc_feature    5194..5866 
                     /label="ColE1"  
     misc_feature    6296..7090 
                     /label="Kan/Neo"  
     misc_feature    7125..7427 
                     /label="SV40 ori"  
     misc_feature    7513..7990 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4194..4200 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4194 
                     /label="MSH6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGACTGGA AATGGCTCTC TTAAAAGGAA AAGCTCTAGG AAGGAAACGC 
     1081 CCTCAGCCAC CAAACAAGCA ACTAGCATTT CATCAGAAAC CAAGAATACT TTGAGAGCTT 
     1141 TCTCTGCCCC TCAAAATTCT GAATCCCAAG CCCACGTTAG TGGAGGTGGT GATGACAGTA 
     1201 GTCGCCCTAC TGTTTGGTAT CATGAAACTT TAGAATGGCT TAAGGAGGAA AAGAGAAGAG 
     1261 ATGAGCACAG GAGGAGGCCT GATCACCCCG ATTTTGATGC ATCTACACTC TATGTGCCTG 
     1321 AGGATTTCCT CAATTCTTGT ACTCCTGGGA TGAGGAAGTG GTGGCAGATT AAGTCTCAGA 
     1381 ACTTTGATCT TGTCATCTGT TACAAGGTGG GGAAATTTTA TGAGCTGTAC CACATGGATG 
     1441 CTCTTATTGG AGTCAGTGAA CTGGGGCTGG TATTCATGAA AGGCAACTGG GCCCATTCTG 
     1501 GCTTTCCTGA AATTGCATTT GGCCGTTATT CAGATTCCCT GGTGCAGAAG GGCTATAAAG 
     1561 TAGCACGAGT GGAACAGACT GAGACTCCAG AAATGATGGA GGCACGATGT AGAAAGATGG 
     1621 CACATATATC CAAGTATGAT AGAGTGGTGA GGAGGGAGAT CTGTAGGATC ATTACCAAGG 
     1681 GTACACAGAC TTACAGTGTG CTGGAAGGTG ATCCCTCTGA GAACTACAGT AAGTATCTTC 
     1741 TTAGCCTCAA AGAAAAAGAG GAAGATTCTT CTGGCCATAC TCGTGCATAT GGTGTGTGCT 
     1801 TTGTTGATAC TTCACTGGGA AAGTTTTTCA TAGGTCAGTT TTCAGATGAT CGCCATTGTT 
     1861 CGAGATTTAG GACTCTAGTG GCACACTATC CCCCAGTACA AGTTTTATTT GAAAAAGGAA 
     1921 ATCTCTCAAA GGAAACTAAA ACAATTCTAA AGAGTTCATT GTCCTGTTCT CTTCAGGAAG 
     1981 GTCTGATACC CGGCTCCCAG TTTTGGGATG CATCCAAAAC TTTGAGAACT CTCCTTGAGG 
     2041 AAGAATATTT TAGGGAAAAG CTAAGTGATG GCATTGGGGT GATGTTACCC CAGGTGCTTA 
     2101 AAGGTATGAC TTCAGAGTCT GATTCCATTG GGTTGACACC AGGAGAGAAA AGTGAATTGG 
     2161 CCCTCTCTGC TCTAGGTGGT TGTGTCTTCT ACCTCAAAAA ATGCCTTATT GATCAGGAGC 
     2221 TTTTATCAAT GGCTAATTTT GAAGAATATA TTCCCTTGGA TTCTGACACA GTCAGCACTA 
     2281 CAAGATCTGG TGCTATCTTC ACCAAAGCCT ATCAACGAAT GGTGCTAGAT GCAGTGACAT 
     2341 TAAACAACTT GGAGATTTTT CTGAATGGAA CAAATGGTTC TACTGAAGGA ACCCTACTAG 
     2401 AGAGGGTTGA TACTTGCCAT ACTCCTTTTG GTAAGCGGCT CCTAAAGCAA TGGCTTTGTG 
     2461 CCCCACTCTG TAACCATTAT GCTATTAATG ATCGTCTAGA TGCCATAGAA GACCTCATGG 
     2521 TTGTGCCTGA CAAAATCTCC GAAGTTGTAG AGCTTCTAAA GAAGCTTCCA GATCTTGAGA 
     2581 GGCTACTCAG TAAAATTCAT AATGTTGGGT CTCCCCTGAA GAGTCAGAAC CACCCAGACA 
     2641 GCAGGGCTAT AATGTATGAA GAAACTACAT ACAGCAAGAA GAAGATTATT GATTTTCTTT 
     2701 CTGCTCTGGA AGGATTCAAA GTAATGTGTA AAATTATAGG GATCATGGAA GAAGTTGCTG 
     2761 ATGGTTTTAA GTCTAAAATC CTTAAGCAGG TCATCTCTCT GCAGACAAAA AATCCTGAAG 
     2821 GTCGTTTTCC TGATTTGACT GTAGAATTGA ACCGATGGGA TACAGCCTTT GACCATGAAA 
     2881 AGGCTCGAAA GACTGGACTT ATTACTCCCA AAGCAGGCTT TGACTCTGAT TATGACCAAG 
     2941 CTCTTGCTGA CATAAGAGAA AATGAACAGA GCCTCCTGGA ATACCTAGAG AAACAGCGCA 
     3001 ACAGAATTGG CTGTAGGACC ATAGTCTATT GGGGGATTGG TAGGAACCGT TACCAGCTGG 
     3061 AAATTCCTGA GAATTTCACC ACTCGCAATT TGCCAGAAGA ATACGAGTTG AAATCTACCA 
     3121 AGAAGGGCTG TAAACGATAC TGGACCAAAA CTATTGAAAA GAAGTTGGCT AATCTCATAA 
     3181 ATGCTGAAGA ACGGAGGGAT GTATCATTGA AGGACTGCAT GCGGCGACTG TTCTATAACT 
     3241 TTGATAAAAA TTACAAGGAC TGGCAGTCTG CTGTAGAGTG TATCGCAGTG TTGGATGTTT 
     3301 TACTGTGCCT GGCTAACTAT AGTCGAGGGG GTGATGGTCC TATGTGTCGC CCAGTAATTC 
     3361 TGTTGCCGGA AGATACCCCC CCCTTCTTAG AGCTTAAAGG ATCACGCCAT CCTTGCATTA 
     3421 CGAAGACTTT TTTTGGAGAT GATTTTATTC CTAATGACAT TCTAATAGGC TGTGAGGAAG 
     3481 AGGAGCAGGA AAATGGCAAA GCCTATTGTG TGCTTGTTAC TGGACCAAAT ATGGGGGGCA 
     3541 AGTCTACGCT TATGAGACAG GCTGGCTTAT TAGCTGTAAT GGCCCAGATG GGTTGTTACG 
     3601 TCCCTGCTGA AGTGTGCAGG CTCACACCAA TTGATAGAGT GTTTACTAGA CTTGGTGCCT 
     3661 CAGACAGAAT AATGTCAGGT GAAAGTACAT TTTTTGTTGA ATTAAGTGAA ACTGCCAGCA 
     3721 TACTCATGCA TGCAACAGCA CATTCTCTGG TGCTTGTGGA TGAATTAGGA AGAGGTACTG 
     3781 CAACATTTGA TGGGACGGCA ATAGCAAATG CAGTTGTTAA AGAACTTGCT GAGACTATAA 
     3841 AATGTCGTAC ATTATTTTCA ACTCACTACC ATTCATTAGT AGAAGATTAT TCTCAAAATG 
     3901 TTGCTGTGCG CCTAGGACAT ATGGCATGCA TGGTAGAAAA TGAATGTGAA GACCCCAGCC 
     3961 AGGAGACTAT TACGTTCCTC TATAAATTCA TTAAGGGAGC TTGTCCTAAA AGCTATGGCT 
     4021 TTAATGCAGC AAGGCTTGCT AATCTCCCAG AGGAAGTTAT TCAAAAGGGA CATAGAAAAG 
     4081 CAAGAGAATT TGAGAAGATG AATCAGTCAC TACGATTATT TCGGGAAGTT TGCCTGGCTA 
     4141 GTGAAAGGTC AACTGTAGAT GCTGAAGCTG TCCATAAATT GCTGACTTTG ATTAAGGAAT 
     4201 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4261 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4321 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4381 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4441 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4501 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4561 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4621 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4681 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4741 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4801 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4861 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4921 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4981 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5041 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5101 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5161 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5221 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5281 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5341 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5401 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5461 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5521 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5581 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5641 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5701 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5761 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5821 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5881 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5941 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6001 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6061 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6121 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6181 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6241 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6301 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6361 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6421 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6481 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6541 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6601 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6661 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6721 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6781 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6841 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6901 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6961 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7021 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7081 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7141 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7201 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7261 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7321 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7381 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7441 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7501 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7561 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7621 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7681 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7741 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7801 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7861 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7921 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7981 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8041 TTTAACGCGA ATTTT
//