LOCUS       RC239923                8046 bp    DNA     circular  SYN 
DEFINITION  NEMF (myc-DDK-tagged) - Human nuclear export mediator factor (NEMF), transcript variant 2
ACCESSION   NM_001301732
VERSION     NM_001301732.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4203..4294 
                     /label="Myc-DDK"  
     misc_feature    4324..4909 
                     /label="PolyA signal"  
     misc_feature    4381..4400 
                     /label="Restriction Enzyme"  
     misc_feature    5185..5857 
                     /label="ColE1"  
     misc_feature    6287..7081 
                     /label="Kan/Neo"  
     misc_feature    7116..7418 
                     /label="SV40 ori"  
     misc_feature    7504..7981 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4185..4191 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4185 
                     /label="NEMF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAGCCGC TTTAGCACCA TTGACCTCCG CGCCGTACTC GCGGAGCTGA 
     1081 ATGCTAGCTT GCTAGGAATG AGAGTAAACA ATGTTTATGA TGTGGATAAT AAGACATACC 
     1141 TTATTCGTCT TCAAAAACCG GACTTTAAAG CTACACTTTT ACTTGAATCT GGCATACGAA 
     1201 TTCATACAAC AGAATTTGAG TGGCCTAAGA ATATGATGCC GTCTAGTTTT GCCATGAAGT 
     1261 GCCGAAAACA TTTGAAGAGT CGGAGATTAG TCAGTGCAAA ACAGCTTGGT GTGGATAGAA 
     1321 TTGTAGATTT TCAATTTGGA AGTGATGAAG CTGCTTACCA TTTAATCATT GAGCTCTATG 
     1381 ATAGGGGGAA CATTGTTCTT ACAGATTATG AGTACGTAAT TTTAAATATT CTAAGGTTTC 
     1441 GAACTGATGA GGCAGATGAT GTTAAATTTG CTGTTCGTGA ACGCTATCCA CTTGATCATG 
     1501 CTAGAGCTGC TGAACCTTTG CTTACTTTGG AAAGGTTGAC TGAAATAGTA GCCAGCGCAC 
     1561 CTAAGGGTGA ACTACTGAAG AGGGTGCTTA ACCCATTACT TCCCTATGGA CCAGCTCTCA 
     1621 TTGAACACTG TCTTTTAGAA AATGGATTCT CGGGTAATGT CAAAGTGGAT GAAAAACTTG 
     1681 AAACTAAAGA TATTGAAAAA GTACTTGTTT CTCTGCAGAA AGCAGAAGAC TATATGAAAA 
     1741 CAACATCCAA CTTCAGTGGG AAGGGATATA TCATTCAGAA AAGAGAAATA AAACCATGCT 
     1801 TGGAAGCAGA TAAACCAGTT GAAGACATAC TGACGTATGA GGAATTTCAT CCTTTCTTGT 
     1861 TTTCTCAACA TTCACAATGT CCATATATAG AATTTGAATC ATTTGACAAG GCGGTGGATG 
     1921 AATTTTATTC CAAGATAGAA GGCCAGAAAA TTGACTTAAA AGCTTTACAA CAGGAAAAGC 
     1981 AAGCATTGAA GAAATTAGAT AATGTTCGAA AGGATCACGA AAACAGATTG GAAGCTCTTC 
     2041 AGCAGGCTCA GGAAATAGAC AAACTGAAAG GAGAGCTCAT AGAAATGAAC CTACAAATAG 
     2101 TTGACAGAGC CATTCAGGTA GTTCGAAGTG CTTTAGCTAA CCAGATAGAT TGGACAGAAA 
     2161 TTGGGTTAAT TGTGAAAGAA GCCCAGGCTC AAGGAGACCC TGTTGCAAGT GCAATCAAAG 
     2221 AATTAAAACT ACAAACAAAC CATGTTACAA TGCTGCTAAG AAATCCATAC TTGTTATCAG 
     2281 AGGAGGAAGA TGATGATGTT GATGGTGACG TCAATGTTGA GAAAAATGAA ACTGAACCAC 
     2341 CAAAAGGAAA AAAGAAAAAA CAAAAGAATA AACAGCTGCA GAAGCCTCAG AAAAATAAGC 
     2401 CCTTACTTGT AGATGTTGAT CTCAGCTTGT CAGCATATGC CAATGCCAAA AAGTATTATG 
     2461 ATCACAAGAG ATATGCTGCT AAGAAAACAC AAAAGACTGT TGAAGCTGCT GAGAAGGCAT 
     2521 TCAAGTCAGC AGAAAAGAAA ACAAAGCAAA CATTAAAAGA AGTTCAGACT GTTACCTCTA 
     2581 TTCAAAAAGC AAGAAAAGTA TATTGGTTTG AGAAATTTCT GTGGTTCATT AGCTCAGAGA 
     2641 ACTATCTAAT TATAGGTGGA CGAGATCAGC AACAGAATGA AATAATTGTG AAAAGATACT 
     2701 TGACACCAGG AGAACCCATC CCCCCACGGA CCTTGACTGA AGCTGGCACA ATGGCACTTT 
     2761 GCTACAGTGC TGCTTGGGAT GCACGAGTTA TCACTAGTGC TTGGTGGGTG TACCATCATC 
     2821 AGGTATCTAA AACAGCACCA ACTGGAGAAT ATTTGACAAC AGGAAGCTTC ATGATAAGAG 
     2881 GAAAAAAGAA TTTTCTTCCT CCCTCATATC TAATGATGGG GTTTAGCTTC CTTTTTAAGG 
     2941 TAGATGAGTC TTGTGTTTGG AGACATCAGG GTGAACGAAA AGTCAGAGTA CAGGATGAAG 
     3001 ACATGGAGAC ACTGGCAAGT TGTACAAGTG AACTCATATC AGAAGAAATG GAACAATTAG 
     3061 ATGGAGGTGA CACGAGCAGT GATGAGGATA AAGAAGAACA TGAAACTCCT GTGGAAGTAG 
     3121 AACTCATGAC TCAGGTTGAC CAAGAGGATA TCACTCTTCA GAGTGGCAGA GATGAACTAA 
     3181 ATGAGGAGCT CATTCAGGAA GAAAGCTCTG AAGACGAAGG AGAATATGAA GAGGTTAGAA 
     3241 AAGATCAGGA TTCTGTTGGT GAAATGAAGG ATGAAGGGGA AGAGACATTA AATTATCCTG 
     3301 ATACTACCAT TGACTTGTCT CACCTTCAAC CCCAAAGGTC CATCCAGAAA TTGGCTTCAA 
     3361 AAGAGGAATC TTCTAATTCT AGTGACAGTA AATCACAGAG CCGGAGACAT TTGTCAGCCA 
     3421 AGGAAAGAAG GGAAATGAAA AAGAAAAAAC TTCCAAGTGA CTCAGGAGAT TTAGAAGCGT 
     3481 TAGAGGGAAA GGATAAAGAA AAAGAAAGTA CTGTACACAT TGAAACTCAT CAGAACACAA 
     3541 GCAAAAATGT TGCGGCTGTG CAGCCAATGA AACGAGGACA AAAGAGTAAA ATGAAAAAAA 
     3601 TGAAAGAAAA ATACAAAGAC CAGGATGAAG AAGACCGTGA ACTTATCATG AAGTTGCTGG 
     3661 GGTCTGCAGG TTCAAACAAA GAAGAAAAAG GGAAGAAGGG GAAGAAAGGA AAAACAAAGG 
     3721 ACGAACCTGT GAAGAAACAG CCCCAGAAAC CTAGAGGTGG ACAGAGGGTC TCTGACAACA 
     3781 TTAAGAAAGA AACTCCGTTC CTTGAGGTTA TAACTCATGA GTTACAAGAC TTTGCTGTAG 
     3841 ATGATCCACA TGATGACAAG GAAGAGCAAG ATCTGGATCA ACAGGGAAAT GAGGAAAACC 
     3901 TATTTGATTC TTTGACAGGC CAGCCACATC CTGAAGATGT ACTACTGTTT GCCATTCCAA 
     3961 TATGTGCCCC TTACACCACC ATGACAAACT ACAAATATAA AGTGAAACTT ACTCCTGGAG 
     4021 TGCAGAAAAA GGGAAAAGCT GCAAAAACAG CCTTGAATAG TTTCATGCAT TCCAAAGAAG 
     4081 CAACAGCAAG AGAAAAAGAC TTATTCCGCA GCGTAAAGGA CACAGATTTA TCAAGAAACA 
     4141 TTCCTGGCAA AGTGAAAGTG TCTGCACCCA ATCTTCTGAA CGTAAAAAGG AAAACGCGTA 
     4201 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4261 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4321 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4381 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4441 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4501 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4561 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4621 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4681 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4741 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4801 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4861 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4921 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4981 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5041 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5101 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5161 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5221 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5281 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5341 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5401 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5461 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5521 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5581 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5641 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5701 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5761 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5821 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5881 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5941 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6001 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6061 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6121 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6181 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6241 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6301 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6361 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6421 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6481 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6541 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6601 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6661 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6721 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6781 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6841 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6901 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6961 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7021 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7081 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7141 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7201 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7261 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7321 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7381 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7441 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7501 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7561 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7621 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7681 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7741 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7801 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7861 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7921 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7981 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8041 AATTTT
//