LOCUS       RC239908                8016 bp    DNA     circular  SYN 
DEFINITION  BLM (myc-DDK-tagged) - Human Bloom syndrome, RecQ helicase-like (BLM), transcript variant 4
ACCESSION   NM_001287248
VERSION     NM_001287248.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4173..4264 
                     /label="Myc-DDK"  
     misc_feature    4294..4879 
                     /label="PolyA signal"  
     misc_feature    4351..4370 
                     /label="Restriction Enzyme"  
     misc_feature    5155..5827 
                     /label="ColE1"  
     misc_feature    6257..7051 
                     /label="Kan/Neo"  
     misc_feature    7086..7388 
                     /label="SV40 ori"  
     misc_feature    7474..7951 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4155..4161 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4155 
                     /label="BLM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GAGCACATCT GTAAATTAAT TGATACTATT CCTGATGATA 
     1081 AACTGAAACT TTTGGATTGT GGGAACGAAC TGCTTCAGCA GCGGAACATA AGAAGGAAAC 
     1141 TTCTAACGGA AGTAGATTTT AATAAAAGTG ATGCCAGTCT TCTTGGCTCA TTGTGGAGAT 
     1201 ACAGGCCTGA TTCACTTGAT GGCCCTATGG AGGGTGATTC CTGCCCTACA GGGAATTCTA 
     1261 TGAAGGAGTT AAATTTTTCA CACCTTCCCT CAAATTCTGT TTCTCCTGGG GACTGTTTAC 
     1321 TGACTACCAC CCTAGGAAAG ACAGGATTCT CTGCCACCAG GAAGAATCTT TTTGAAAGGC 
     1381 CTTTATTCAA TACCCATTTA CAGAAGTCCT TTGTAAGTAG CAACTGGGCT GAAACACCAA 
     1441 GACTAGGAAA AAAAAATGAA AGCTCTTATT TCCCAGGAAA TGTTCTCACA AGCACTGCTG 
     1501 TGAAAGATCA GAATAAACAT ACTGCTTCAA TAAATGACTT AGAAAGAGAA ACCCAACCTT 
     1561 CCTATGATAT TGATAATTTT GACATAGATG ACTTTGATGA TGATGATGAC TGGGAAGACA 
     1621 TAATGCATAA TTTAGCAGCC AGCAAATCTT CCACAGCTGC CTATCAACCC ATCAAGGAAG 
     1681 GTCGGCCAAT TAAATCAGTA TCAGAAAGAC TTTCCTCAGC CAAGACAGAC TGTCTTCCAG 
     1741 TGTCATCTAC TGCTCAAAAT ATAAACTTCT CAGAGTCAAT TCAGAATTAT ACTGACAAGT 
     1801 CAGCACAAAA TTTAGCATCC AGAAATCTGA AACATGAGCG TTTCCAAAGT CTTAGTTTTC 
     1861 CTCATACAAA GGAAATGATG AAGATTTTTC ATAAAAAATT TGGCCTGCAT AATTTTAGAA 
     1921 CTAATCAGCT AGAGGCGATC AATGCTGCAC TGCTTGGTGA AGACTGTTTT ATCCTGATGC 
     1981 CGACTGGAGG TGGTAAGAGT TTGTGTTACC AGCTCCCTGC CTGTGTTTCT CCTGGGGTCA 
     2041 CTGTTGTCAT TTCTCCCTTG AGATCACTTA TCGTAGATCA AGTCCAAAAG CTGACTTCCT 
     2101 TGGATATTCC AGCTACATAT CTGACAGGTG ATAAGACTGA CTCAGAAGCT ACAAATATTT 
     2161 ACCTCCAGTT ATCAAAAAAA GACCCAATCA TAAAACTTCT ATATGTCACT CCAGAAAAGA 
     2221 TCTGTGCAAG TAACAGACTC ATTTCTACTC TGGAGAATCT CTATGAGAGG AAGCTCTTGG 
     2281 CACGTTTTGT TATTGATGAA GCACATTGTG TCAGTCAGTG GGGACATGAT TTTCGTCAAG 
     2341 ATTACAAAAG AATGAATATG CTTCGCCAGA AGTTTCCTTC TGTTCCGGTG ATGGCTCTTA 
     2401 CGGCCACAGC TAATCCCAGG GTACAGAAGG ACATCCTGAC TCAGCTGAAG ATTCTCAGAC 
     2461 CTCAGGTGTT TAGCATGAGC TTTAACAGAC ATAATCTGAA ATACTATGTA TTACCGAAAA 
     2521 AGCCTAAAAA GGTGGCATTT GATTGCCTAG AATGGATCAG AAAGCACCAC CCATATGATT 
     2581 CAGGGATAAT TTACTGCCTC TCCAGGCGAG AATGTGACAC CATGGCTGAC ACGTTACAGA 
     2641 GAGATGGGCT CGCTGCTCTT GCTTACCATG CTGGCCTCAG TGATTCTGCC AGAGATGAAG 
     2701 TGCAGCAGAA GTGGATTAAT CAGGATGGCT GTCAGGTTAT CTGTGCTACA ATTGCATTTG 
     2761 GAATGGGGAT TGACAAACCG GACGTGCGAT TTGTGATTCA TGCATCTCTC CCTAAATCTG 
     2821 TGGAGGGTTA CTACCAAGAA TCTGGCAGAG CTGGAAGAGA TGGGGAAATA TCTCACTGCC 
     2881 TGCTTTTCTA TACCTATCAT GATGTGACCA GACTGAAAAG ACTTATAATG ATGGAAAAAG 
     2941 ATGGAAACCA TCATACAAGA GAAACTCACT TCAATAATTT GTATAGCATG GTACATTACT 
     3001 GTGAAAATAT AACGGAATGC AGGAGAATAC AGCTTTTGGC CTACTTTGGT GAAAATGGAT 
     3061 TTAATCCTGA TTTTTGTAAG AAACACCCAG ATGTTTCTTG TGATAATTGC TGTAAAACAA 
     3121 AGGATTATAA AACAAGAGAT GTGACTGACG ATGTGAAAAG TATTGTAAGA TTTGTTCAAG 
     3181 AACATAGTTC ATCACAAGGA ATGAGAAATA TAAAACATGT AGGTCCTTCT GGAAGATTTA 
     3241 CTATGAATAT GCTGGTCGAC ATTTTCTTGG GGAGTAAGAG TGCAAAAATC CAGTCAGGTA 
     3301 TATTTGGAAA AGGATCTGCT TATTCACGAC ACAATGCCGA AAGACTTTTT AAAAAGCTGA 
     3361 TACTTGACAA GATTTTGGAT GAAGACTTAT ATATCAATGC CAATGACCAG GCGATCGCTT 
     3421 ATGTGATGCT CGGAAATAAA GCCCAAACTG TACTAAATGG CAATTTAAAG GTAGACTTTA 
     3481 TGGAAACAGA AAATTCCAGC AGTGTGAAAA AACAAAAAGC GTTAGTAGCA AAAGTGTCTC 
     3541 AGAGGGAAGA GATGGTTAAA AAATGTCTTG GAGAACTTAC AGAAGTCTGC AAATCTCTGG 
     3601 GGAAAGTTTT TGGTGTCCAT TACTTCAATA TTTTTAATAC CGTCACTCTC AAGAAGCTTG 
     3661 CAGAATCTTT ATCTTCTGAT CCTGAGGTTT TGCTTCAAAT TGATGGTGTT ACTGAAGACA 
     3721 AACTGGAAAA ATATGGTGCG GAAGTGATTT CAGTATTACA GAAATACTCT GAATGGACAT 
     3781 CGCCAGCTGA AGACAGTTCC CCAGGGATAA GCCTGTCCAG CAGCAGAGGC CCCGGAAGAA 
     3841 GTGCCGCTGA GGAGCTCGAC GAGGAAATAC CCGTATCTTC CCACTACTTT GCAAGTAAAA 
     3901 CCAGAAATGA AAGGAAGAGG AAAAAGATGC CAGCCTCCCA AAGGTCTAAG AGGAGAAAAA 
     3961 CTGCTTCCAG TGGTTCCAAG GCAAAGGGGG GGTCTGCCAC ATGTAGAAAG ATATCTTCCA 
     4021 AAACGAAATC CTCCAGCATC ATTGGATCCA GTTCAGCCTC ACATACTTCT CAAGCGACAT 
     4081 CAGGAGCCAA TAGCAAATTG GGGATTATGG CTCCACCGAA GCCTATAAAT AGACCGTTTC 
     4141 TTAAGCCTTC ATATGCATTC TCAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4201 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4261 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4321 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4381 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4441 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4501 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4561 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4621 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4681 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4741 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4801 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4861 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4921 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4981 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5041 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5101 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5161 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5221 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5281 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5341 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5401 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5461 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5521 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5581 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5641 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5701 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5761 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5821 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5881 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5941 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6001 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6061 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6121 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6181 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6241 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6301 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6361 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6421 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6481 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6541 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6601 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6661 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6721 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6781 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6841 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6901 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6961 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7021 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7081 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7141 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7201 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7261 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7321 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7381 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7441 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7501 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7561 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7621 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7681 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7741 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7801 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7861 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7921 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7981 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
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