LOCUS       RC239885                7929 bp    DNA     circular  SYN 
DEFINITION  EPHA5 (myc-DDK-tagged) - Human EPH receptor A5 (EPHA5), transcript variant 4
ACCESSION   NM_001281766
VERSION     NM_001281766.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4086..4177 
                     /label="Myc-DDK"  
     misc_feature    4207..4792 
                     /label="PolyA signal"  
     misc_feature    4264..4283 
                     /label="Restriction Enzyme"  
     misc_feature    5068..5740 
                     /label="ColE1"  
     misc_feature    6170..6964 
                     /label="Kan/Neo"  
     misc_feature    6999..7301 
                     /label="SV40 ori"  
     misc_feature    7387..7864 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4068..4074 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4068 
                     /label="EPHA5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGGCTCG GGGCCCCGGG GTGCGGGACG CCGGCGGCCC CCAAGCGGCG 
     1081 GCGGCGACAC CCCCATCACC CCAGCGTCCC TGGCCGGCTG CTACTCTGCA CCTCGACGGG 
     1141 CTCCCCTCTG GACGTGCCTT CTCCTGTGCG CCGCACTCCG GACCCTCCTG GCCAGCCCCA 
     1201 GCAACGAAGT GAATTTATTG GATTCACGCA CTGTCATGGG GGACCTGGGA TGGATTGCTT 
     1261 TTCCAAAAAA TGGGTGGGAA GAGATTGGTG AAGTGGATGA AAATTATGCC CCTATCCACA 
     1321 CATACCAAGT ATGCAAAGTG ATGGAACAGA ATCAGAATAA CTGGCTTTTG ACCAGTTGGA 
     1381 TCTCCAATGA AGGTGCTTCC AGAATCTTCA TAGAACTCAA ATTTACCCTG CGGGACTGCA 
     1441 ACAGCCTTCC TGGAGGACTG GGGACCTGTA AGGAAACCTT TAATATGTAT TACTTTGAGT 
     1501 CAGATGATCA GAATGGGAGA AACATCAAGG AAAACCAATA CATCAAAATT GATACCATTG 
     1561 CTGCCGATGA AAGCTTTACA GAACTTGATC TTGGTGACCG TGTTATGAAA CTGAATACAG 
     1621 AGGTCAGAGA TGTAGGACCT CTAAGCAAAA AGGGATTTTA TCTTGCTTTT CAAGATGTTG 
     1681 GTGCTTGCAT TGCTCTGGTT TCTGTGCGTG TATACTATAA AAAATGCCCT TCTGTGGTAC 
     1741 GACACTTGGC TGTCTTCCCT GACACCATCA CTGGAGCTGA TTCTTCCCAA TTGCTCGAAG 
     1801 TGTCAGGCTC CTGTGTCAAC CATTCTGTGA CCGATGAACC TCCCAAAATG CACTGCAGCG 
     1861 CCGAAGGGGA GTGGCTGGTG CCCATCGGGA AATGCATGTG CAAGGCAGGA TATGAAGAGA 
     1921 AAAATGGCAC CTGTCAAGTG TGCAGACCTG GGTTCTTCAA AGCCTCACCT CACATCCAGA 
     1981 GCTGCGGCAA ATGTCCACCT CACAGTTATA CCCATGAGGA AGCTTCAACC TCTTGTGTCT 
     2041 GTGAAAAGGA TTATTTCAGG AGAGAGTCTG ATCCACCCAC AATGGCATGC ACAAGACCCC 
     2101 CCTCTGCTCC TCGGAATGCC ATCTCAAATG TTAATGAAAC TAGTGTCTTT CTGGAATGGA 
     2161 TTCCGCCTGC TGACACTGGT GGAAGGAAAG ACGTGTCATA TTATATTGCA TGCAAGAAGT 
     2221 GCAACTCCCA TGCAGGTGTG TGTGAGGAGT GTGGCGGTCA TGTCAGGTAC CTTCCCCGGC 
     2281 AAAGCGGCCT GAAAAACACC TCTGTCATGA TGGTGGATCT ACTCGCTCAC ACAAACTATA 
     2341 CCTTTGAGAT TGAGGCAGTG AATGGAGTGT CCGACTTGAG CCCAGGAGCC CGGCAGTATG 
     2401 TGTCTGTAAA TGTAACCACA AATCAAGCAG CTCCATCTCC AGTCACCAAT GTGAAAAAAG 
     2461 GGAAAATTGC AAAAAACAGC ATCTCTTTGT CTTGGCAAGA ACCAGATCGT CCCAATGGAA 
     2521 TCATCCTAGA GTATGAAATC AAGTATTTTG AAAAGGACCA AGAGACCAGC TACACGATTA 
     2581 TCAAATCTAA AGAGACAACT ATTACTGCAG AGGGCTTGAA ACCAGCTTCA GTTTATGTCT 
     2641 TCCAAATTCG AGCACGTACA GCAGCAGGCT ATGGTGTCTT CAGTCGAAGA TTTGAGTTTG 
     2701 AAACCACCCC AGTGTCAGTT GCAGCATCCA GCGATCAAAG CCAGATTCCT GTAATTGCTG 
     2761 TGTCTGTGAC AGTGGGAGTC ATTTTGTTGG CAGTGGTTAT CGGCGTCCTC CTCAGTGGAA 
     2821 GGCGGTGTGG CTACAGCAAA GCAAAACAAG ATCCAGAAGA GGAAAAGATG CATTTTCATA 
     2881 ATGGGCACAT TAAACTGCCA GGAGTAAGAA CTTACATTGA TCCACATACC TATGAGGATC 
     2941 CCAATCAAGC TGTCCACGAA TTTGCTAAGG AGATAGAAGC ATCATGTATC ACCATTGAGA 
     3001 GAGTTATTGG AGCAGGTGAA TTTGGTGAAG TTTGTAGTGG ACGTTTGAAA CTACCAGGAA 
     3061 AAAGAGAATT ACCTGTGGCT ATCAAAACCC TTAAAGTAGG CTATACTGAA AAGCAACGCA 
     3121 GAGATTTCCT AGGTGAAGCA AGTATCATGG GACAGTTTGA TCATCCTAAC ATCATCCATT 
     3181 TAGAAGGTGT GGTGACCAAA AGTAAACCAG TGATGATCGT GACAGAGTAT ATGGAGAATG 
     3241 GCTCTTTAGA TACATTTTTG AAGAAAAACG ATGGGCAGTT CACTGTGATT CAGCTTGTTG 
     3301 GCATGCTGAG AGGTATCTCT GCAGGAATGA AGTACCTTTC TGACATGGGC TATGTGCATA 
     3361 GAGATCTTGC TGCCAGAAAC ATCTTAATCA ACAGTAACCT TGTGTGCAAA GTGTCTGACT 
     3421 TTGGACTTTC CCGGGTACTG GAAGATGATC CCGAGGCAGC CTACACCACA AGGGGAGGAA 
     3481 AAATTCCAAT CAGATGGACT GCCCCAGAAG CAATAGCTTT CCGAAAGTTT ACTTCTGCCA 
     3541 GTGATGTCTG GAGTTATGGA ATAGTAATGT GGGAAGTTGT GTCTTATGGA GAGAGACCCT 
     3601 ACTGGGAGAT GACCAATCAA GATGTGATTA AAGCGGTAGA GGAAGGCTAT CGTCTGCCAA 
     3661 GCCCCATGGA TTGTCCTGCT GCTCTCTATC AGTTAATGCT GGATTGCTGG CAGAAAGAGC 
     3721 GAAATAGCAG GCCCAAGTTT GATGAAATAG TCAACATGTT GGACAAGCTG ATACGTAACC 
     3781 CAAGTAGTCT GAAGACGCTG GTTAATGCAT CCTGCAGAGT ATCTAATTTA TTGGCAGAAC 
     3841 ATAGCCCACT AGGATCTGGG GCCTACAGAT CAGTAGGTGA ATGGCTAGAG GCAATCAAGA 
     3901 TGGGCCGGTA TACAGAGATT TTCATGGAAA ATGGATACAG TTCAATGGAC GCTGTGGCTC 
     3961 AGGTGACCTT GGAGGATTTG AGACGGCTTG GAGTGACTCT TGTCGGTCAC CAGAAGAAGA 
     4021 TCATGAACAG CCTTCAAGAA ATGAAGGTGC AGCTGGTAAA CGGAATGGTG CCATTGACGC 
     4081 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4141 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4201 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4261 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4321 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4381 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4441 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4501 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4561 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4621 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4681 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4741 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4801 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4861 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4921 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4981 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5041 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5101 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5161 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5221 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5281 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5341 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5401 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5461 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5521 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5581 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5641 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5701 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5761 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5821 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5881 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5941 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6001 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6061 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6121 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6181 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6241 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6301 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6361 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6421 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6481 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6541 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6601 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6661 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6721 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6781 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6841 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6901 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6961 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7021 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7081 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7141 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7201 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7261 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7321 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7381 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7441 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7501 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7561 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7621 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7681 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7741 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7801 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7861 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7921 GCGAATTTT
//