LOCUS       RC239872                7893 bp    DNA     circular  SYN 
DEFINITION  USP38 (myc-DDK-tagged) - Human ubiquitin specific peptidase 38 (USP38), transcript variant 2
ACCESSION   NM_001290325
VERSION     NM_001290325.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4050..4141 
                     /label="Myc-DDK"  
     misc_feature    4171..4756 
                     /label="PolyA signal"  
     misc_feature    4228..4247 
                     /label="Restriction Enzyme"  
     misc_feature    5032..5704 
                     /label="ColE1"  
     misc_feature    6134..6928 
                     /label="Kan/Neo"  
     misc_feature    6963..7265 
                     /label="SV40 ori"  
     misc_feature    7351..7828 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4032..4038 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4032 
                     /label="USP38"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAAGATC CTGGAGGGCC TTGTGAGTTC CTCGCATCCC CTGCCCCTCA 
     1081 AGCGGGTGAT TGTGCGGAAG GTGGTGGAAT CGGCGGAGCA CTGGCTAGAC GAGGCGCAGT 
     1141 GCGAGGCCAT GTTTGACCTG ACGACCCGGC TCATCCTGGA GGGCCAGGAC CCTTTCCAGC 
     1201 GGCAGGTGGG GCACCAGGTG CTGGAGGCCT ACGCACGATA CCACCGGCCA GAGTTCGAGT 
     1261 CCTTCTTCAA CAAGACCTTC GTGTTGGGCC TCCTTCATCA GGGCTACCAC TCTCTGGACA 
     1321 GGAAGGATGT AGCCATCCTG GACTACATTC ACAACGGCCT GAAGCTGATT ATGAGCTGTC 
     1381 CGTCGGTGCT GGATCTCTTT AGCCTCCTGC AGGTAGAGGT GTTACGGATG GTGTGTGAGA 
     1441 GGCCGGAGCC GCAGCTCTGT GCCCGACTGA GCGACCTTCT GACCGACTTT GTGCAATGCA 
     1501 TCCCCAAGGG GAAATTGTCC ATCACGTTCT GTCAACAGCT GGTTCGAACG ATAGGCCATT 
     1561 TCCAGTGCGT GTCCACCCAG GAAAGAGAGC TGCGGGAATA TGTCTCCCAG GTGACAAAAG 
     1621 TGAGTAACTT GCTGCAGAAC ATCTGGAAGG CCGAGCCTGC CACACTACTG CCTTCCCTGC 
     1681 AAGAAGTTTT TGCAAGCATC TCTTCCACAG ATGCATCATT TGAACCTTCT GTAGCATTGG 
     1741 CAAGCCTTGT GCAGCATATT CCTCTTCAGA TGATTACAGT TCTCATCAGG AGCCTTACTA 
     1801 CGGATCCAAA TGTAAAAGAT GCAAGTATGA CCCAAGCCCT TTGCAGAATG ATTGACTGGC 
     1861 TATCCTGGCC ATTGGCTCAG CATGTGGATA CATGGGTAAT TGCACTCCTG AAAGGACTGG 
     1921 CAGCTGTCCA GAAGTTTACT ATTTTGATAG ATGTTACTTT GCTGAAAATA GAACTGGTTT 
     1981 TTAATCGACT TTGGTTTCCT CTTGTGAGAC CTGGTGCTCT TGCAGTTCTT TCTCACATGC 
     2041 TGCTTAGCTT TCAGCATTCT CCAGAGGCGT TCCATTTGAT TGTTCCTCAT GTGGTTAATT 
     2101 TGGTTCATTC TTTCAAAAAT GATGGTCTGC CTTCAAGTAC AGCCTTCTTA GTACAATTAA 
     2161 CAGAATTGAT ACACTGTATG ATGTATCATT ATTCTGGATT TCCAGATCTC TATGAACCTA 
     2221 TTCTGGAGGC AATAAAGGAT TTTCCTAAGC CCAGTGAAGA GAAGATTAAG TTAATTCTCA 
     2281 ATCAAAGTGC CTGGACTTCT CAATCCAATT CTTTGGCGTC TTGCTTGTCT AGACTTTCTG 
     2341 GAAAATCTGA AACTGGGAAA ACTGGTCTTA TTAACCTAGG AAATACATGT TATATGAACA 
     2401 GTGTTATACA AGCCTTGTTT ATGGCCACAG ATTTCAGGAG ACAAGTATTA TCTTTAAATC 
     2461 TAAATGGGTG CAATTCATTA ATGAAAAAAT TACAGCATCT TTTTGCCTTT CTGGCCCATA 
     2521 CACAGAGGGA AGCATACGCA CCTCGGATAT TCTTTGAGGC TTCCAGACCT CCATGGTTTA 
     2581 CTCCCAGATC ACAGCAAGAC TGTTCTGAAT ACCTCAGATT TCTCCTTGAC AGGCTCCATG 
     2641 AAGAAGAAAA GATCTTGAAA GTTCAGGCCT CACACAAGCC TTCTGAAATT CTGGAATGCA 
     2701 GTGAAACTTC TTTACAGGAA GTAGCTAGTA AAGCAGCAGT ACTAACAGAG ACCCCTCGTA 
     2761 CAAGTGACGG TGAGAAGACT TTAATAGAAA AAATGTTTGG AGGAAAACTA CGAACTCACA 
     2821 TACGTTGTTT GAACTGCAGG AGTACCTCAC AAAAAGTGGA AGCCTTTACA GATCTTTCGC 
     2881 TTGCCTTTTG TCCTTCCTCT TCTTTGGAAA ACATGTCTGT CCAAGATCCA GCATCATCAC 
     2941 CCAGTATACA AGATGGTGGT CTAATGCAAG CCTCTGTACC CGGTCCTTCA GAAGAACCAG 
     3001 TAGTTTATAA TCCAACAACA GCTGCCTTCA TCTGTGACTC ACTTGTGAAT GAAAAAACCA 
     3061 TAGGCAGTCC TCCTAATGAG TTTTACTGTT CTGAAAACAC TTCTGTCCCT AACGAATCTA 
     3121 ACAAGATTCT TGTTAATAAA GATGTACCTC AGAAACCAGG AGGTGAAACC ACACCTTCAG 
     3181 TAACTGACTT ACTAAATTAT TTTTTGGCTC CAGAGATTCT TACTGGTGAT AACCAATATT 
     3241 ATTGTGAAAA CTGTGCCTCT CTGCAAAATG CTGAGAAAAC TATGCAAATC ACGGAGGAAC 
     3301 CTGAATACCT TATTCTTACT CTCCTGAGAT TTTCATATGA TCAGAAGTAT CATGTGAGAA 
     3361 GGAAAATTTT AGACAATGTA TCACTGCCAC TGGTTTTGGA GTTGCCAGTT AAAAGAATTA 
     3421 CTTCTTTCTC TTCATTGTCA GAAAGTTGGT CTGTAGATGT TGACTTCACT GATCTTAGTG 
     3481 AGAACCTTGC TAAAAAATTA AAGCCTTCAG GGACTGATGA AGCTTCCTGC ACAAAATTGG 
     3541 TGCCCTATCT ATTAAGTTCC GTTGTGGTTC ACTCTGGTAT ATCCTCTGAA AGTGGGCATT 
     3601 ACTATTCTTA TGCCAGGAAT ATCACAAGTA CAGACTCTTC ATATCAGATG TACCACCAGT 
     3661 CTGAGGCTCT GGCATTAGCA TCCTCCCAGA GTCATTTACT AGGGAGAGAT AGTCCCAGTG 
     3721 CAGTTTTTGA ACAGGATTTG GAAAATAAGG AAATGTCAAA AGAATGGTTT TTATTTAATG 
     3781 ACAGTAGAGT GACATTTACT TCATTTCAGT CAGTCCAGAA AATTACGAGC AGGTTTCCAA 
     3841 AGGACACAGC TTATGTGCTT TTGTATAAAA AACAGCATAG TACTAATGGT TTAAGTGGTA 
     3901 ATAACCCAAC CAGTGGACTC TGGATAAATG GAGACCCACC TCTACAGAAA GAACTTATGG 
     3961 ATGCTATAAC AAAAGACAAT AAACTATATT TACAGGTAAG TTGGAAATAC AAGCTTTATT 
     4021 TGTTGAAAAT ATTAAACAAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4081 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4141 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4201 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4261 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4321 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4381 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4441 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4501 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4561 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4621 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4681 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4741 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4801 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4861 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4921 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4981 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5041 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5101 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5161 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5221 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5281 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5341 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5401 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5461 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5521 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5581 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5641 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5701 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5761 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5821 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5881 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5941 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6001 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6061 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6121 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6181 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6241 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6301 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6361 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6421 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6481 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6541 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6601 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6661 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6721 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6781 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6841 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6901 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6961 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7021 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7081 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7141 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7201 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7261 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7321 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7381 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7441 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7501 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7561 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7621 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7681 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7741 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7801 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7861 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//