LOCUS       RC239864                7878 bp    DNA     circular  SYN 
DEFINITION  POLR1B (myc-DDK-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 6
ACCESSION   NM_001282777
VERSION     NM_001282777.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4033..4124 
                     /label="Myc-DDK"  
     misc_feature    4154..4739 
                     /label="PolyA signal"  
     misc_feature    4211..4230 
                     /label="Restriction Enzyme"  
     misc_feature    5015..5687 
                     /label="ColE1"  
     misc_feature    6117..6911 
                     /label="Kan/Neo"  
     misc_feature    6946..7248 
                     /label="SV40 ori"  
     misc_feature    7334..7811 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4008..4015 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4008 
                     /label="POLR1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAAATCC AAGCTTTGCA ACTTACGTAA CCTTCCCCCA CAAGCCCTCA 
     1081 TTGAGCACCA TGAGGAGGCA GAGGAAATGG GGGGCTATTT TATAATCAAT GGCATTGAAA 
     1141 AAGTCATCCG AATGTTGATT ATGCCTCGGA GAAATTTTCC CATTGCAATG ATAAGACCAA 
     1201 AATGGAAAAC CAGAGGGCCT GGTTATACTC AGTATGGAGT TTCAATGCAC TGTGTGAGGG 
     1261 AAGAACATTC CGCTGTCAAT ATGAACCTCC ACTACTTGGA AAATGGCACT GTTATGTTGA 
     1321 ACTTTATTTA CCGAAAAGAA CTGTTCTTTC TTCCTTTGGG ATTTGCACTT AAGGCACTTG 
     1381 TCAGCTTTTC TGATTATCAG ATCTTTCAGG AGCTCATCAA AGGAAAAGAG GATGATTCTT 
     1441 TCCTTAGGAA CTCTGTTTCT CAGATGTTAA GGATTGTAAT GGAAGAGGGT TGTTCGACAC 
     1501 AAAAACAGGT CCTTAACTAC CTAGGTGAAT GCTTCAGAGT AAAACTCAAT GTTCCTGACT 
     1561 GGTACCCAAA TGAGCAAGCT GCGGAGTTCC TGTTTAACCA GTGCATCTGT ATCCACTTGA 
     1621 AATCCAATAC TGAAAAGTTT TATATGCTTT GTCTCATGAC GCGAAAGCTC TTTGCTTTAG 
     1681 CCAAAGGAGA GTGCATGGAG GACAATCCTG ATAGTTTGGT GAACCAGGAA GTCCTCACAC 
     1741 CGGGTCAGCT CTTCCTTATG TTCCTGAAGG AAAAACTGGA AGGTTGGTTA GTGTCTATTA 
     1801 AAATAGCTTT TGATAAGAAG GCTCAGAAGA CCAGTGTTTC CATGAACACT GACAATTTGA 
     1861 TGAGGATTTT TACAATGGGC ATAGACCTTA CAAAACCATT TGAATACCTT TTTGCTACTG 
     1921 GGAATCTGCG TTCTAAAACA GGTCTTGGCC TCCTACAAGA TTCTGGACTT TGTGTTGTGG 
     1981 CTGACAAGCT GAACTTCATA CGCTACCTCT CCCATTTCCG CTGCGTGCAC AGAGGGGCTG 
     2041 ATTTTGCCAA GATGAGGACC ACCACAGTAC GCAGGCTGCT GCCAGAGTCC TGGGGCTTCC 
     2101 TTTGTCCCGT GCATACCCCA GACGGGGAGC CCTGTGGCCT GATGAACCAC CTAACTGCCG 
     2161 TATGTGAGGT TGTCACACAG TTTGTGTATA CGGCATCTAT TCCAGCTTTA CTGTGCAACT 
     2221 TGGGGGTCAC TCCCATTGAT GGAGCTCCCC ACCGATCATA CAGTGAGTGC TACCCTGTCC 
     2281 TGCTGGACGG TGTCATGGTT GGCTGGGTGG ATAAGGATCT TGCTCCAGGC ATCGCAGATT 
     2341 CTCTTCGTCA TTTTAAGGTG TTGAGAGAGA AAAGAATTCC TCCCTGGATG GAAGTGGTCC 
     2401 TTATACCCAT GACAGGAAAA CCAAGTCTGT ACCCAGGATT GTTCCTTTTT ACCACTCCTT 
     2461 GTAGACTGGT ACGGCCTGTG CAGAACTTAG CATTGGGCAA AGAAGAGCTA ATTGGAACTA 
     2521 TGGAACAGAT CTTCATGAAT GTCGCTATCT TTGAGGATGA AGTTTTTGCT GGAGTTACCA 
     2581 CACACCAGGA ACTCTTTCCA CACAGCCTGC TGAGTGTGAT TGCCAACTTC ATCCCTTTCT 
     2641 CTGATCACAA CCAGAGTCCA CGGAACATGT ACCAATGCCA GATGGGTAAG CAAACTATGG 
     2701 GCTTTCCACT TCTCACTTAT CAAGACCGAT CGGATAACAA ACTGTATCGT CTTCAGACTC 
     2761 CTCAGAGTCC CTTGGTGAGA CCCTCCATGT ATGATTATTA TGACATGGAT AACTATCCAA 
     2821 TTGGGACCAA TGCCATCGTT GCTGTGATTT CTTACACTGG CTATGATATG GAAGATGCCA 
     2881 TGATTGTGAA TAAGGCCTCT TGGGAACGAG GCTTTGCCCA TGGAAGTGTC TACAAGTCTG 
     2941 AGTTCATAGA CCTCTCTGAA AAAATTAAAC AAGGAGATAG TAGCCTGGTG TTTGGCATCA 
     3001 AACCTGGTGA CCCACGCGTT CTGCAGAAGT TAGATGACGA TGGATTGCCG TTTATAGGAG 
     3061 CAAAACTGCA GTACGGAGAT CCGTATTACA GCTACCTCAA CCTCAACACC GGGGAAAGTT 
     3121 TTGTGATGTA CTATAAGAGT AAAGAAAATT GTGTTGTGGA TAACATCAAA GTGTGCAGTA 
     3181 ATGACACTGG GAGTGGAAAA TTCAAGTGTG TTTGCATCAC TATGAGAGTG CCTCGGAACC 
     3241 CAACTATCGG AGATAAATTT GCCAGTCGCC ATGGGCAGAA GGGCATTTTA AGCAGATTGT 
     3301 GGCCGGCTGA GGACATGCCT TTTACTGAGA GTGGGATGGT CCCAGACATT CTGTTCAATC 
     3361 CCCATGGTTT TCCATCCCGC ATGACCATTG GGATGTTAAT TGAGAGTATG GCCGGGAAGT 
     3421 CTGCAGCTTT GCATGGTCTC TGCCATGATG CTACACCCTT CATCTTCTCA GAGGAGAACT 
     3481 CGGCCTTAGA ATACTTTGGT GAGATGTTAA AGGCTGCTGG CTACAATTTC TATGGCACCG 
     3541 AGAGGTTATA TAGTGGCATC AGTGGGCTAG AACTGGAAGC AGACATCTTC ATAGGAGTGG 
     3601 TTTATTATCA GCGCTTACGC CATATGGTCT CAGACAAATT TCAAGTAAGG ACAACTGGAG 
     3661 CCCGAGACAG AGTCACCAAC CAGCCTATTG GGGGAAGAAA TGTCCAGGGT GGAATCCGTT 
     3721 TTGGGGAGAT GGAACGGGAT GCGCTTTTAG CTCATGGTAC ATCTTTTCTC CTTCATGACC 
     3781 GCCTCTTCAA CTGCTCAGAT CGGTCGGTAG CCCATGTGTG TGTGAAGTGT GGCAGTTTAC 
     3841 TCTCTCCACT GTTGGAGAAG CCACCCCCTT CTTGGTCTGC CATGCGCAAC AGAAAATACA 
     3901 ACTGTACTCT GTGTAGTCGC AGTGACACTA TCGATACTGT TTCTGTGCCT TATGTTTTTC 
     3961 GGTATTTTGT AGCTGAACTG GCAGCTATGA ACATCAAAGT GAAACTGGAT GTTGTTAGCG 
     4021 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4081 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4141 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4201 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4261 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4321 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4381 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4441 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4501 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4561 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4621 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4681 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4741 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4801 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4861 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4921 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4981 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5041 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5101 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5161 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5221 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5281 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5341 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5401 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5461 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5521 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5581 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5641 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5701 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5761 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5821 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5881 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5941 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6001 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6061 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6121 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6181 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6241 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6301 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6361 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6421 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6481 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6541 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6601 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6661 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6721 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6781 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6841 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6901 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6961 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7021 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7081 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7141 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7201 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7261 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7321 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7381 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7441 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7501 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7561 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7621 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7681 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7741 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7801 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7861 AAATTTAACG CGAATTTT
//