LOCUS       RC239856                7842 bp    DNA     circular  SYN 
DEFINITION  BNC1 (myc-DDK-tagged) - Human basonuclin 1 (BNC1), transcript variant 2
ACCESSION   NM_001301206
VERSION     NM_001301206.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3999..4090 
                     /label="Myc-DDK"  
     misc_feature    4120..4705 
                     /label="PolyA signal"  
     misc_feature    4177..4196 
                     /label="Restriction Enzyme"  
     misc_feature    4981..5653 
                     /label="ColE1"  
     misc_feature    6083..6877 
                     /label="Kan/Neo"  
     misc_feature    6912..7214 
                     /label="SV40 ori"  
     misc_feature    7300..7777 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3981..3987 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3981 
                     /label="BNC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTGTAGA AACATGTTCT TCTCATTTAA GGCATCTCTT TGTGGCTGCG 
     1081 GGGCTGCCAC CGCTCCGAGT CTGACAGCTA TCAGCTGTAC TCTGAACTGT AGTTGCCAAA 
     1141 GTTTCAAACC CGGGAAAATA AACCACCGTC AGTGTGACCA ATGCAAGCAT GGATGGGTGG 
     1201 CCCACGCTCT AAGTAAGCTA AGGATCCCCC CCATGTATCC AACAAGCCAG GTGGAGATTG 
     1261 TCCAGTCCAA TGTAGTGTTT GATATTAGCA GCCTCATGCT CTATGGGACC CAGGCCATCC 
     1321 CCGTTCGCCT AAAAATCCTA CTGGACCGGC TCTTCAGTGT GTTGAAGCAA GATGAGGTTC 
     1381 TCCAGATCCT CCATGCCTTG GACTGGACAC TTCAGGATTA TATCCGTGGA TACGTACTGC 
     1441 AGGATGCATC AGGAAAGGTG TTGGATCACT GGAGCATCAT GACCAGTGAG GAAGAAGTGG 
     1501 CCACCTTGCA GCAGTTCCTT CGTTTTGGAG AGACCAAATC TATAGTTGAA CTCATGGCAA 
     1561 TTCAAGAGAA AGAAGAGCAA TCCATCATCA TACCACCTTC CACAGCAAAT GTAGATATCA 
     1621 GGGCTTTCAT CGAGAGCTGC AGTCACAGGA GTTCTAGCCT CCCCACTCCT GTGGACAAAG 
     1681 GAAACCCCAG CAGTATACAC CCCTTTGAGA ACCTCATAAG CAACATGACT TTCATGCTGC 
     1741 CTTTCCAGTT CTTCAACCCT CTGCCTCCTG CACTGATAGG GTCATTGCCC GAACAATATA 
     1801 TGTTGGAGCA GGGTCATGAC CAAAGTCAGG ACCCCAAACA GGAAGTCCAT GGGCCCTTCC 
     1861 CTGACAGCAG CTTCTTAACT TCCAGTTCCA CACCATTTCA GGTTGAAAAA GATCAGTGTT 
     1921 TAAACTGTCC GGATGCTATT ACTAAAAAAG AAGACAGCAC CCATTTAAGT GACTCCAGCT 
     1981 CATACAACAT TGTCACTAAG TTTGAAAGGA CACAGTTATC CCCTGAGGCC AAAGTGAAGC 
     2041 CTGAGAGGAA TAGCCTTGGT ACAAAGAAGG GCCGGGTGTT CTGCACTGCA TGTGAGAAGA 
     2101 CCTTCTATGA CAAAGGCACC CTCAAAATCC ACTACAATGC CGTCCACTTG AAGATCAAGC 
     2161 ATAAGTGCAC CATCGAAGGG TGTAACATGG TGTTCAGCTC CCTAAGGAGC CGGAATCGCC 
     2221 ATAGCGCCAA CCCCAACCCT CGGCTGCACA TGCCAATGAA CAGAAATAAC CGGGACAAAG 
     2281 ACCTCAGGAA CAGCCTGAAC CTGGCCAGCT CTGAGAACTA CAAGTGCCCA GGTTTCACAG 
     2341 TGACGTCCCC AGACTGTAGG CCTCCTCCCA GCTACCCTGG TTCAGGAGAG GATTCCAAAG 
     2401 GCCAACCAGC CTTCCCAAAC ATTGGGCAAA ATGGTGTGCT TTTTCCCAAC CTAAAGACAG 
     2461 TCCAGCCAGT CCTTCCTTTC TACCGCAGTC CAGCCACGCC TGCCGAGGTA GCAAACACGC 
     2521 CTGGGATACT CCCTTCCCTC CCGCTGTTGT CCTCTTCAAT CCCAGAACAG CTCATTTCAA 
     2581 ACGAAATGCC ATTTGATGCC CTTCCCAAGA AGAAATCCAG GAAGTCCAGT ATGCCTATCA 
     2641 AAATAGAGAA AGAAGCTGTG GAAATAGCTA ATGAGAAAAG ACACAACCTC AGCTCAGATG 
     2701 AAGACATGCC CCTACAGGTG GTCAGTGAAG ATGAGCAGGA GGCCTGCAGT CCTCAGTCAC 
     2761 ACAGAGTATC TGAGGAGCAG CATGTACAGT CAGGAGGCTT AGGGAAGCCT TTCCCTGAAG 
     2821 GGGAGAGGCC CTGCCATCGT GAATCAGTAA TTGAGTCCAG TGGAGCCATC AGCCAAACCC 
     2881 CTGAGCAGGC CACACACAAT TCAGAGAGGG AGACTGAGCA GACACCAGCA TTGATCATGG 
     2941 TGCCAAGGGA GGTCGAGGAT GGTGGCCATG AACACTACTT CACACCTGGG ATGGAACCCC 
     3001 AAGTTCCTTT TTCTGACTAC ATGGAACTGC AGCAGCGCCT GCTGGCTGGG GGACTCTTCA 
     3061 GTGCTTTGTC CAACAGGGGA ATGGCTTTTC CTTGTCTTGA AGATTCTAAA GAACTGGAGC 
     3121 ACGTGGGTCA GCATGCATTA GCAAGGCAGA TAGAAGAAAA TCGCTTCCAG TGTGACATCT 
     3181 GCAAGAAGAC CTTTAAAAAT GCTTGTAGTG TGAAAATTCA TCACAAGAAT ATGCATGTCA 
     3241 AAGAAATGCA CACATGCACA GTGGAGGGCT GTAATGCTAC CTTTCCCTCC CGCAGGAGCA 
     3301 GAGACAGACA CAGCTCAAAC CTAAACCTCC ACCAAAAAGC ATTGAGCCAG GAAGCATTGG 
     3361 AGAGTAGTGA AGATCATTTC CGTGCAGCTT ACCTTCTGAA AGATGTGGCT AAGGAAGCCT 
     3421 ATCAGGATGT GGCTTTTACA CAGCAAGCCT CCCAGACATC TGTCATCTTC AAAGGAACAA 
     3481 GTCGAATGGG CAGTCTGGTT TACCCAATAA CGCAAGTCCA CAGTGCCAGC CTGGAGAGCT 
     3541 ACAACTCTGG CCCCTTGAGC GAGGGCACCA TCCTGGATTT GAGCACTACC TCGAGCATGA 
     3601 AGTCAGAGAG TAGCAGCCAT TCTTCCTGGG ACTCTGACGG GGTGAGTGAG GAAGGCACTG 
     3661 TGCTTATGGA GGACAGTGAT GGGAACTGTG AAGGGTCGAG CCTTGTCCCT GGGGAAGATG 
     3721 AGTACCCCAT CTGTGTCCTG ATGGAGAAGG CTGACCAGAG CCTTGCTAGC CTGCCTTCTG 
     3781 GGTTGCCCAT AACCTGTCAT CTCTGCCAAA AGACATACAG TAACAAAGGG ACCTTTAGGG 
     3841 CCCACTACAA AACTGTGCAC CTCCGGCAGC TCCACAAATG CAAAGTACCA GGCTGCAACA 
     3901 CCATGTTTTC GTCTGTTCGC AGTCGAAACA GACACAGCCA GAATCCCAAC CTGCACAAAA 
     3961 GCCTGGCCTC ATCTCCAAGT CACCTCCAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4021 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4081 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4141 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4201 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4261 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4321 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4381 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4441 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4501 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4561 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4621 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4681 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4741 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4801 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4861 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4921 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4981 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5041 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5101 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5161 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5221 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5281 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5341 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5401 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5461 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5521 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5581 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5641 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5701 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5761 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5821 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5881 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5941 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6001 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6061 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6121 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6181 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6241 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6301 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6361 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6421 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6481 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6541 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6601 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6661 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6721 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6781 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6841 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6901 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6961 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7021 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7081 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7141 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7261 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7321 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7381 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7441 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7501 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7561 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7621 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7681 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7741 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7801 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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