LOCUS       RC239850                7818 bp    DNA     circular  SYN 
DEFINITION  HERC4 (myc-DDK-tagged) - Human HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3
ACCESSION   NM_001278185
VERSION     NM_001278185.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3975..4066 
                     /label="Myc-DDK"  
     misc_feature    4096..4681 
                     /label="PolyA signal"  
     misc_feature    4153..4172 
                     /label="Restriction Enzyme"  
     misc_feature    4957..5629 
                     /label="ColE1"  
     misc_feature    6059..6853 
                     /label="Kan/Neo"  
     misc_feature    6888..7190 
                     /label="SV40 ori"  
     misc_feature    7276..7753 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3957..3963 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3957 
                     /label="HERC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTGCTGG GGAAATGCAT CCTTTGGGCA GCTAGGTTTG GGTGGAATTG 
     1081 ATGAAGAAAT TGTACTAGAG CCCAGAAAAA GTGACTTCTT TATAAATAAA AGGGTCCGAG 
     1141 ATGTAGGATG TGGACTCAGA CATACTGTGT TTGTTCTGGA TGATGGAACA GTGTACACAT 
     1201 GTGGATGTAA TGATCTAGGA CAGCTAGGTC ATGAAAAATC CAGAAAGAAA CCAGAGCAGG 
     1261 TTGTTGCCCT GGATGCCCAA AATATTGTAG CTGTTTCATG TGGAGAAGCT CATACGTTAG 
     1321 CGCTAAATGA CAAAGGCCAG GTGTATGCTT GGGGTCTCGA TTCTGATGGA CAGCTTGGCC 
     1381 TGGTAGGATC AGAGGAATGC ATCAGAGTAC CCAGAAATAT TAAAAGTTTG TCAGATATCC 
     1441 AGATTGTACA GGTTGCTTGT GGTTACTATC ATTCACTTGC ACTTTCTAAA GCAAGTGAAG 
     1501 TCTTCTGTTG GGGACAGAAT AAATATGGCC AATTGGGTTT AGGTACTGAC TGTAAAAAGC 
     1561 AAACTTCACC GCAGCTGCTT AAGTCTTTGC TTGGAATCCC TTTCATGCAA GTTGCAGCAG 
     1621 GAGGAGCCCA TAGTTTTGTA CTCACCCTTT CTGGAGCTAT CTTTGGATGG GGACGCAACA 
     1681 AGTTTGGTCA GCTAGGTCTT AATGATGAAA ATGATAGGTA TGTTCCTAAT TTACTAAAGT 
     1741 CACTAAGATC TCAGAAAATA GTTTATATTT GTTGTGGAGA AGATCATACT GCTGCTCTAA 
     1801 CCAAGGAAGG TGGAGTGTTT ACTTTTGGAG CTGGAGGGTA TGGTCAGTTG GGCCATAATT 
     1861 CTACCAGTCA TGAAATAAAC CCAAGGAAAG TTTTTGAACT TATGGGAAGC ATTGTCACTG 
     1921 AGATTGCTTG TGGACGGCAG CACACTTCTG CTTTTGTTCC TTCATCAGGA CGAATTTACT 
     1981 CTTTTGGGCT TGGTGGTAAT GGGCAGCTGG GAACCGGTTC AACAAGCAAC AGGAAAAGCC 
     2041 CCTTTACTGT AAAAGGAAAT TGGTACCCCT ATAATGGGCA GTGTCTACCA GATATTGATT 
     2101 CTGAAGAATA TTTCTGTGTA AAAAGAATTT TCTCAGGGGG AGATCAAAGC TTTTCACATT 
     2161 ACTCTAGTCC CCAGAACTGT GGGCCACCAG ATGACTTCAG ATGTCCCAAT CCGACAAAGC 
     2221 AGATCTGGAC AGTGAATGAA GCTCTAATTC AGAAATGGCT GAGCTATCCT TCTGGAAGGT 
     2281 TTCCTGTGGA GATAGCCAAT GAGATAGATG GAACGTTTTC TTCCTCTGGT TGCCTAAATG 
     2341 GAAGTTTTTT AGCTGTTAGC AATGATGATC ACTATAGAAC AGGTACCAGA TTTTCAGGGG 
     2401 TTGATATGAA TGCTGCTAGG CTTTTATTCC ACAAACTTAT ACAACCTGAT CATCCGCAGA 
     2461 TATCTCAGCA GGTGGCAGCT AGTTTGGAAA AGAATCTTAT TCCTAAACTG ACTAGCTCCT 
     2521 TACCTGATGT TGAAGCATTG AGGTTTTATC TTACTCTACC AGAATGTCCC CTGATGAGTG 
     2581 ATTCCAACAA TTTCACAACA ATAGCAATTC CCTTTGGTAC AGCTCTTGTG AACCTAGAAA 
     2641 AGGCACCACT GAAAGTACTT GAAAACTGGT GGTCAGTACT TGAACCTCCA CTATTCCTCA 
     2701 AGATAGTAGA ACTTTTTAAG GAAGTTGTGG TACATCTTTT GAAACTCTAC AAGATCGGTA 
     2761 TTCCCCCTTC TGAAAGAAGA ATTTTCAACA GTTTTCTTCA TACTGCATTA AAGGTTTTAG 
     2821 AAATACTACA TAGGGTAAAT GAGAAAATGG GACAGATTAT ACAGTATGAT AAATTTTATA 
     2881 TACATGAAGT ACAAGAATTG ATAGACATAA GAAATGATTA TATCAACTGG GTCCAACAGC 
     2941 AGGCCTATGG AATGGATGTC AACCATGGAT TAACTGAGTT GGCAGATATC CCTGTTACAA 
     3001 TCTGTACATA TCCATTTGTA TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAGACCGATG 
     3061 CAGTCTTACA GATGCAGATG GCTATTGATC AGGCCCACAG GCAGAATGTC TCCTCTCTTT 
     3121 TTCTCCCAGT GATTGAATCT GTGAATCCCT GCTTAATTCT AGTGGTGCGT AGAGAAAATA 
     3181 TTGTAGGAGA TGCAATGGAA GTCCTTAGGA AAACAAAGAA CATAGATTAC AAGAAGCCAC 
     3241 TCAAGGTTAT ATTTGTTGGA GAAGATGCTG TGGATGCAGG AGGGGTGCGC AAAGAATTTT 
     3301 TCTTGCTCAT CATGAGGGAA TTATTGGATC CTAAATACGG CATGTTTAGG TATTATGAAG 
     3361 ATTCCAGGCT CATTTGGTTT TCTGATAAGA TCACAGTTGA AAACTTTGGT GCAACAGAAG 
     3421 TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAAGAGT 
     3481 TTGTCGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTA TTTGATGCTT 
     3541 TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAAGTCCT TCTGCTCTTT CAGCCTAATG 
     3601 AACTACAAGC AATGGTCATT GGAAATACAA ATTATGATTG GAAGGAACTG GAAAAGAATA 
     3661 CAGAATACAA AGGGGAATAT TGGGCAGAAC ATCCTACGAT AAAAATTTTT TGGGAAGTAT 
     3721 TTCACGAATT ACCATTGGAA AAGAAGAAAC AGTTTCTGTT ATTTTTGACA GGTAGTGATC 
     3781 GCATTCCTAT TCTTGGTATG AAGAGTCTGA AACTAGTCAT CCAGTCCACA GGAGGTGGTG 
     3841 AGGAGTATCT CCCAGTTTCC CATACTTGTT TTAATCTTCT GGATCTTCCA AAATATACAG 
     3901 AAAAAGAAAC TCTACGCTCT AAACTGATCC AAGCTATTGA TCACAATGAA GGCTTCAGTT 
     3961 TAATAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4021 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4081 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4141 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4201 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4261 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4321 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4381 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4441 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4501 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4561 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4621 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4681 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4741 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4801 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4861 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4921 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4981 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5041 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5101 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5161 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5221 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5281 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5341 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5401 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5461 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5521 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5581 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5641 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5701 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5761 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5821 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5881 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5941 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6001 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6061 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6121 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6181 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6241 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6301 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6361 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6421 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6481 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6541 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6601 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6661 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6721 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6781 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6841 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6901 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6961 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7021 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7081 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7141 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7201 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7261 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7321 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7381 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7441 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7501 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7561 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7621 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7681 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7741 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7801 AAATTTAACG CGAATTTT
//