LOCUS       RC239847                7824 bp    DNA     circular  SYN 
DEFINITION  CACHD1 (myc-DDK-tagged) - Human cache domain containing 1 (CACHD1), transcript variant 2
ACCESSION   NM_001293274
VERSION     NM_001293274.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3979..4070 
                     /label="Myc-DDK"  
     misc_feature    4100..4685 
                     /label="PolyA signal"  
     misc_feature    4157..4176 
                     /label="Restriction Enzyme"  
     misc_feature    4961..5633 
                     /label="ColE1"  
     misc_feature    6063..6857 
                     /label="Kan/Neo"  
     misc_feature    6892..7194 
                     /label="SV40 ori"  
     misc_feature    7280..7757 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3954..3961 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3954 
                     /label="CACHD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCACCTTT GTTAGCAGCG TGAAGTCTTC AGACAGTCCT ACCCAGCACG 
     1081 CAGTGGGATT CCAAAAGGCA TTTCAGCTGA TTCGAAGTAC AAACAATAAC ACAAAGTTCC 
     1141 AAGCAAATAC AGACATGGTC ATCATTTACC TGTCAGCTGG CATTACATCA AAGGACTCTT 
     1201 CGGAAGAAGA TAAAAAAGCG ACTCTCCAAG TCATCAATGA AGAAAATAGC TTTCTAAACA 
     1261 ACTCTGTAAT GATTCTCACC TATGCCCTCA TGAACGATGG GGTGACTGGT TTGAAAGAGC 
     1321 TGGCTTTTCT GAGGGATCTA GCTGAACAGA ATTCAGGGAA GTACGGTGTG CCAGACCGGA 
     1381 TGGCCTTGCC TGTGATTAAG GGCAGCATGA TGGTGCTGAA TCAGTTGAGC AACCTGGAGA 
     1441 CCACAGTGGG CAGGTTCTAC ACAAACCTTC CCAACCGGAT GATTGATGAA GCCGTCTTCA 
     1501 GCCTGCCCTT CTCTGATGAG ATGGGAGATG GTTTGATAAT GACTGTGAGT AAACCCTGTT 
     1561 ATTTTGGAAA CCTACTTCTG GGAATTGTAG GTGTGGACGT GAATCTGGCT TACATTCTTG 
     1621 AAGACGTGAC GTATTACCAA GACTCTTTGG CTTCCTATAC TTTTCTCATA GACGACAAAG 
     1681 GATATACACT TATGCACCCA TCTCTTACCA GGCCATATTT ATTGTCAGAG CCCCCACTTC 
     1741 ATACTGACAT CATACATTAT GAAAATATTC CAAAATTTGA ATTAGTTCGG CAAAATATCC 
     1801 TAAGCCTCCC TCTGGGCAGC CAGATTATCG CAGTCCCTGT GAACTCATCC CTGTCTTGGC 
     1861 ACATAAACAA GCTGAGAGAA ACTGGAAAGG AAGCCTACAA TGTTAGCTAT GCCTGGAAGA 
     1921 TGGTACAAGA CACTTCCTTT ATTCTGTGTA TTGTGGTGAT ACAACCAGAA ATACCTGTGA 
     1981 AACAACTGAA GAACCTCAAC ACTGTTCCCA GCAGCAAGCT GCTGTACCAC CGGCTGGATC 
     2041 TCCTTGGCCA GCCCAGTGCT TGCCTCCACT TCAAACAGCT GGCAACCCTA GAAAGTCCCA 
     2101 CCATCATGCT GTCTGCTGGC AGCTTTTCCT CCCCCTATGA GCACCTCAGC CAGCCAGAGA 
     2161 CAAAGCGCAT GGTAGAGCAC TACACCGCCT ATCTCAGCGA CAACACCCGC CTCATTGCTA 
     2221 ACCCGGGCCT CAAATTCTCT GTCAGAAATG AAGTAATGGC TACCAGCCAC GTCACAGATG 
     2281 AATGGATGAC ACAAATGGAA ATGAGTAGCC TGAACACTTA CATTGTCCGC CGTTACATAG 
     2341 CAACACCCAA TGGCGTCCTC AGAATTTATC CTGGTTCCCT CATGGACAAA GCATTTGATC 
     2401 CCACTAGGAG ACAATGGTAT CTCCATGCAG TAGCTAATCC AGGGTTGATT TCTTTGACTG 
     2461 GTCCTTACTT AGATGTTGGA GGAGCTGGTT ATGTTGTGAC AATCAGTCAC ACAATTCATT 
     2521 CATCCAGTAC ACAGCTGTCT TCTGGGCACA CTGTGGCTGT GATGGGCATT GACTTCACAC 
     2581 TCAGATACTT CTACAAAGTT CTGATGGACC TATTACCTGT CTGTAACCAA GATGGTGGCA 
     2641 ACAAAATAAG GTGCTTCATA ATGGAGGACA GGGGTTATCT GGTGGCGCAC CCGACTCTCA 
     2701 TCGACCCCAA AGGACATGCA CCTGTGGAGC AGCAGCACAT CACCCACAAG GAGCCCCTGG 
     2761 TAGCAAATGA TATCCTCAAC CACCCCAACT TTGTAAAGAA AAACCTGTGC AACAGCTTCA 
     2821 GTGACAGAAC GGTCCAGAGG TTTTATAAAT TCAACACCAG CCTTGCGGGG GATTTGACGA 
     2881 ACCTTGTGCA TGGCAGCCAC TGTTCCAAAT ACAGATTAGC AAGGATCCCA GGAACCAACG 
     2941 CGTTTGTTGG CATTGTCAAC GAAACCTGCG ACTCTCTTGC CTTCTGTGCC TGCAGCATGG 
     3001 TGGACCGACT CTGTCTCAAC TGTCACCGAA TGGAACAAAA TGAATGTGAA TGTCCTTGTG 
     3061 AGTGCCCTCT AGAGGTCAAT GAGTGCACTG GCAACCTCAC CAATGCAGAG AACCGAAACC 
     3121 CCAGCTGCGA GGTCCACCAG GAGCCGGTGA CATACACAGC TATTGACCCT GGCCTGCAAG 
     3181 ATGCTCTTCA CCAGTGTGTC AACAGCAGGT GCAGTCAGAG GCTGGAAAGT GGGGACTGTT 
     3241 TTGGGGTGCT GGATTGTGAA TGGTGCATGG TGGACAGTGA TGGAAAGACT CACCTGGACA 
     3301 AACCCTACTG TGCCCCCCAG AAAGAATGCT TCGGGGGGAT TGTGGGAGCC AAAAGTCCCT 
     3361 ACGTTGATGA CATGGGAGCA ATAGGTGATG AGGTGATCAC ATTAAACATG ATTAAAAGCG 
     3421 CCCCTGTGGG TCCTGTGGCT GGAGGGATCA TGGGATGCAT CATGGTCTTG GTCCTGGCGG 
     3481 TGTATGCCTA CCGCCACCAG ATTCATCGCC GGAGCCATCA GCATATGTCT CCTCTTGCTG 
     3541 CCCAAGAAAT GTCAGTGCGT ATGTCCAACC TGGAGAATGA CAGAGATGAA AGGGACGACG 
     3601 ACAGCCACGA AGACAGAGGC ATCATCAGCA ACACTCGGTT TATAGCTGCG GTCATCGAAC 
     3661 GACATGCACA CAGTCCAGAA AGAAGGCGCC GCTACTGGGG TCGATCAGGA ACAGAAAGTG 
     3721 ATCATGGTTA CAGCACCATG AGCCCACAGG AGGACAGTGA AAATCCTCCA TGCAACAATG 
     3781 ACCCCTTGTC AGCCGGGGTC GATGTGGGAA ACCATGATGA GGACTTAGAC CTGGATACCC 
     3841 CCCCTCAGAC TGCTGCCCTA CTAAGTCACA AGTTCCACCA CTACCGGTCA CACCACCCTA 
     3901 CACTTCATCA TAGCCACCAC TTACAGGCGG CCGTCACGGT ACACACTGTC GATGCAGAAT 
     3961 GCAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4021 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4081 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4141 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4201 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4261 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4321 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4381 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4441 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4501 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4561 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4621 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4681 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4741 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5521 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5581 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5641 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5701 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5761 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5821 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5881 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5941 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6001 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6061 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6121 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6181 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6241 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6301 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6361 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6421 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6481 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6541 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6601 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6661 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6721 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6781 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6841 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6901 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6961 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7021 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7261 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7321 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7381 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7441 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7501 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7561 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7621 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7681 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7741 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7801 TAACAAAAAT TTAACGCGAA TTTT
//