LOCUS       RC239815                7746 bp    DNA     circular  SYN 
DEFINITION  POLR1B (myc-DDK-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 4
ACCESSION   NM_001282774
VERSION     NM_001282774.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3901..3992 
                     /label="Myc-DDK"  
     misc_feature    4022..4607 
                     /label="PolyA signal"  
     misc_feature    4079..4098 
                     /label="Restriction Enzyme"  
     misc_feature    4883..5555 
                     /label="ColE1"  
     misc_feature    5985..6779 
                     /label="Kan/Neo"  
     misc_feature    6814..7116 
                     /label="SV40 ori"  
     misc_feature    7202..7679 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3876..3883 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3876 
                     /label="POLR1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCTGGC AGCCGGTGGC GGAACCTGCC CAGCGGGCCT AGCCTAAAGC 
     1081 ACTTGACTGA CCCCTCTTAT GGAATCCCGC GGGAACAGCA AAAGGCAGCG TTGCAGGAGC 
     1141 TGACGCGGGC GCACGTGGAG TCCTTCAACT ACGCTGTGCA CGAGGGTCTC GGCCTCGCGG 
     1201 TGCAGGCTAT ACCTCCCTTT GAATTTGCTT TCAAAGATGA GCGTATCTCT TTTACTATTC 
     1261 TGGATGCTGT TATCAGTCCA CCTACAGTTC CAAAAGGGAC CATCTGCAAA GAGGCCAATG 
     1321 TTTATCCAGC AGAATGCCGG GGCCGAAGGA GTACCTACCG TGGGAAGTTG ACAGCTGATA 
     1381 TCAACTGGGC AGTGAATGGA ATCTCAAAAG GAATCATTAA GCAGTTTCTT GGCTATGTTC 
     1441 CCATCATGGT GAAATCCAAG CTTTGCAACT TACGTAACCT TCCCCCACAA GCCCTCATTG 
     1501 AGCACCATGA GGAGGCAGAG GAAATGGGGG GCTATTTTAT AATCAATGGC ATTGAAAAAG 
     1561 TCATCCGAAT GTTGATTATG CCTCGGAGAA ATTTTCCCAT TGCAATGATA AGACCAAAAT 
     1621 GGAAAACCAG AGGGCCTGGT TATACTCAGT ATGGAGTTTC AATGCACTGT GTGAGGGAAG 
     1681 AACATTCCGC TGTCAATATG AACCTCCACT ACTTGGAAAA TGGCACTGTT ATGTTGAACT 
     1741 TTATTTACCG AAAAGAACTG TTCTTTCTTC CTTTGGGATT TGCACTTAAG GCACTTGTCA 
     1801 GCTTTTCTGA TTATCAGATC TTTCAGGAGC TCATCAAAGG AAAAGAGGAT GATTCTTTCC 
     1861 TTAGGAACTC TGTTTCTCAG ATGTTAAGGA TTGTAATGGA AGAGGGTTGT TCGACACAAA 
     1921 AACAGGTCCT TAACTACCTA GGTGAATGCT TCAGAGTAAA ACTCAATGTT CCTGACTGGT 
     1981 ACCCAAATGA GCAAGCTGCG GAGTTCCTGT TTAACCAGTG CATCTGTATC CACTTGAAAT 
     2041 CCAATACTGA AAAGTTTTAT ATGCTTTGTC TCATGACGCG AAAGCTCTTT GCTTTAGCCA 
     2101 AAGGAGAGTG CATGGAGGAC AATCCTGATA GTTTGGTGAA CCAGGAAGTC CTCACACCGG 
     2161 GTCAGCTCTT CCTTATGTTC CTGAAGGGGT CACTCCCATT GATGGAGCTC CCCACCGATC 
     2221 ATACAGTGTT GAGAGAGAAA AGAATTCCTC CCTGGATGGA AGTGGTCCTT ATACCCATGA 
     2281 CAGGAAAACC AAGTCTGTAC CCAGGATTGT TCCTTTTTAC CACTCCTTGT AGACTGGTAC 
     2341 GGCCTGTGCA GAACTTAGCA TTGGGCAAAG AAGAGCTAAT TGGAACTATG GAACAGATCT 
     2401 TCATGAATGT CGCTATCTTT GAGGATGAAG TTTTTGCTGG AGTTACCACA CACCAGGAAC 
     2461 TCTTTCCACA CAGCCTGCTG AGTGTGATTG CCAACTTCAT CCCTTTCTCT GATCACAACC 
     2521 AGAGTCCACG GAACATGTAC CAATGCCAGA TGGGTAAGCA AACTATGGGC TTTCCACTTC 
     2581 TCACTTATCA AGACCGATCG GATAACAAAC TGTATCGTCT TCAGACTCCT CAGAGTCCCT 
     2641 TGGTGAGACC CTCCATGTAT GATTATTATG ACATGGATAA CTATCCAATT GGGACCAATG 
     2701 CCATCGTTGC TGTGATTTCT TACACTGGCT ATGATATGGA AGATGCCATG ATTGTGAATA 
     2761 AGGCCTCTTG GGAACGAGGC TTTGCCCATG GAAGTGTCTA CAAGTCTGAG TTCATAGACC 
     2821 TCTCTGAAAA AATTAAACAA GGAGATAGTA GCCTGGTGTT TGGCATCAAA CCTGGTGACC 
     2881 CACGCGTTCT GCAGAAGTTA GATGACGATG GATTGCCGTT TATAGGAGCA AAACTGCAGT 
     2941 ACGGAGATCC GTATTACAGC TACCTCAACC TCAACACCGG GGAAAGTTTT GTGATGTACT 
     3001 ATAAGAGTAA AGAAAATTGT GTTGTGGATA ACATCAAAGT GTGCAGTAAT GACACTGGGA 
     3061 GTGGAAAATT CAAGTGTGTT TGCATCACTA TGAGAGTGCC TCGGAACCCA ACTATCGGAG 
     3121 ATAAATTTGC CAGTCGCCAT GGGCAGAAGG GCATTTTAAG CAGATTGTGG CCGGCTGAGG 
     3181 ACATGCCTTT TACTGAGAGT GGGATGGTCC CAGACATTCT GTTCAATCCC CATGGTTTTC 
     3241 CATCCCGCAT GACCATTGGG ATGTTAATTG AGAGTATGGC CGGGAAGTCT GCAGCTTTGC 
     3301 ATGGTCTCTG CCATGATGCT ACACCCTTCA TCTTCTCAGA GGAGAACTCG GCCTTAGAAT 
     3361 ACTTTGGTGA GATGTTAAAG GCTGCTGGCT ACAATTTCTA TGGCACCGAG AGGTTATATA 
     3421 GTGGCATCAG TGGGCTAGAA CTGGAAGCAG ACATCTTCAT AGGAGTGGTT TATTATCAGC 
     3481 GCTTACGCCA TATGGTCTCA GACAAATTTC AAGTAAGGAC AACTGGAGCC CGAGACAGAG 
     3541 TCACCAACCA GCCTATTGGG GGAAGAAATG TCCAGGGTGG AATCCGTTTT GGGGAGATGG 
     3601 AACGGGATGC GCTTTTAGCT CATGGTACAT CTTTTCTCCT TCATGACCGC CTCTTCAACT 
     3661 GCTCAGATCG GTCGGTAGCC CATGTGTGTG TGAAGTGTGG CAGTTTACTC TCTCCACTGT 
     3721 TGGAGAAGCC ACCCCCTTCT TGGTCTGCCA TGCGCAACAG AAAATACAAC TGTACTCTGT 
     3781 GTAGTCGCAG TGACACTATC GATACTGTTT CTGTGCCTTA TGTTTTTCGG TATTTTGTAG 
     3841 CTGAACTGGC AGCTATGAAC ATCAAAGTGA AACTGGATGT TGTTAGCGGA CCGACGCGTA 
     3901 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3961 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4021 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4081 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4141 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4201 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4261 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4321 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4381 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4441 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4501 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4561 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4621 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4681 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4741 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4801 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4861 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4921 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4981 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5041 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5101 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5161 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5221 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5281 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5341 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5401 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5461 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5521 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5581 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5641 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5701 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5761 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5821 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5881 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5941 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6001 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6061 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6121 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6181 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6241 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6301 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6361 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6421 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6481 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6541 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6601 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6661 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6721 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6781 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6841 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6901 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6961 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7021 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7081 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7141 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7201 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7261 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7321 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7381 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7441 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7501 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7561 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7621 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7681 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7741 AATTTT
//