LOCUS       RC239790                7662 bp    DNA     circular  SYN 
DEFINITION  POLR1B (myc-DDK-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 5
ACCESSION   NM_001282776
VERSION     NM_001282776.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3817..3908 
                     /label="Myc-DDK"  
     misc_feature    3938..4523 
                     /label="PolyA signal"  
     misc_feature    3995..4014 
                     /label="Restriction Enzyme"  
     misc_feature    4799..5471 
                     /label="ColE1"  
     misc_feature    5901..6695 
                     /label="Kan/Neo"  
     misc_feature    6730..7032 
                     /label="SV40 ori"  
     misc_feature    7118..7595 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3792..3799 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3792 
                     /label="POLR1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACTGTGTG AGGGAAGAAC ATTCCGCTGT CAATATGAAC CTCCACTACT 
     1081 TGGAAAATGG CACTGTTATG TTGAACTTTA TTTACCGAAA AGAACTGTTC TTTCTTCCTT 
     1141 TGGGATTTGC ACTTAAGGCA CTTGTCAGCT TTTCTGATTA TCAGATCTTT CAGGAGCTCA 
     1201 TCAAAGGAAA AGAGGATGAT TCTTTCCTTA GGAACTCTGT TTCTCAGATG TTAAGGATTG 
     1261 TAATGGAAGA GGGTTGTTCG ACACAAAAAC AGGTCCTTAA CTACCTAGGT GAATGCTTCA 
     1321 GAGTAAAACT CAATGTTCCT GACTGGTACC CAAATGAGCA AGCTGCGGAG TTCCTGTTTA 
     1381 ACCAGTGCAT CTGTATCCAC TTGAAATCCA ATACTGAAAA GTTTTATATG CTTTGTCTCA 
     1441 TGACGCGAAA GCTCTTTGCT TTAGCCAAAG GAGAGTGCAT GGAGGACAAT CCTGATAGTT 
     1501 TGGTGAACCA GGAAGTCCTC ACACCGGGTC AGCTCTTCCT TATGTTCCTG AAGGAAAAAC 
     1561 TGGAAGGTTG GTTAGTGTCT ATTAAAATAG CTTTTGATAA GAAGGCTCAG AAGACCAGTG 
     1621 TTTCCATGAA CACTGACAAT TTGATGAGGA TTTTTACAAT GGGCATAGAC CTTACAAAAC 
     1681 CATTTGAATA CCTTTTTGCT ACTGGGAATC TGCGTTCTAA AACAGGTCTT GGCCTCCTAC 
     1741 AAGATTCTGG ACTTTGTGTT GTGGCTGACA AGCTGAACTT CATACGCTAC CTCTCCCATT 
     1801 TCCGCTGCGT GCACAGAGGG GCTGATTTTG CCAAGATGAG GACCACCACA GTACGCAGGC 
     1861 TGCTGCCAGA GTCCTGGGGC TTCCTTTGTC CCGTGCATAC CCCAGACGGG GAGCCCTGTG 
     1921 GCCTGATGAA CCACCTAACT GCCGTATGTG AGGTTGTCAC ACAGTTTGTG TATACGGCAT 
     1981 CTATTCCAGC TTTACTGTGC AACTTGGGGG TCACTCCCAT TGATGGAGCT CCCCACCGAT 
     2041 CATACAGTGA GTGCTACCCT GTCCTGCTGG ACGGTGTCAT GGTTGGCTGG GTGGATAAGG 
     2101 ATCTTGCTCC AGGCATCGCA GATTCTCTTC GTCATTTTAA GGTGTTGAGA GAGAAAAGAA 
     2161 TTCCTCCCTG GATGGAAGTG GTCCTTATAC CCATGACAGG AAAACCAAGT CTGTACCCAG 
     2221 GATTGTTCCT TTTTACCACT CCTTGTAGAC TGGTACGGCC TGTGCAGAAC TTAGCATTGG 
     2281 GCAAAGAAGA GCTAATTGGA ACTATGGAAC AGATCTTCAT GAATGTCGCT ATCTTTGAGG 
     2341 ATGAAGTTTT TGCTGGAGTT ACCACACACC AGGAACTCTT TCCACACAGC CTGCTGAGTG 
     2401 TGATTGCCAA CTTCATCCCT TTCTCTGATC ACAACCAGAG TCCACGGAAC ATGTACCAAT 
     2461 GCCAGATGGG TAAGCAAACT ATGGGCTTTC CACTTCTCAC TTATCAAGAC CGATCGGATA 
     2521 ACAAACTGTA TCGTCTTCAG ACTCCTCAGA GTCCCTTGGT GAGACCCTCC ATGTATGATT 
     2581 ATTATGACAT GGATAACTAT CCAATTGGGA CCAATGCCAT CGTTGCTGTG ATTTCTTACA 
     2641 CTGGCTATGA TATGGAAGAT GCCATGATTG TGAATAAGGC CTCTTGGGAA CGAGGCTTTG 
     2701 CCCATGGAAG TGTCTACAAG TCTGAGTTCA TAGACCTCTC TGAAAAAATT AAACAAGGAG 
     2761 ATAGTAGCCT GGTGTTTGGC ATCAAACCTG GTGACCCACG CGTTCTGCAG AAGTTAGATG 
     2821 ACGATGGATT GCCGTTTATA GGAGCAAAAC TGCAGTACGG AGATCCGTAT TACAGCTACC 
     2881 TCAACCTCAA CACCGGGGAA AGTTTTGTGA TGTACTATAA GAGTAAAGAA AATTGTGTTG 
     2941 TGGATAACAT CAAAGTGTGC AGTAATGACA CTGGGAGTGG AAAATTCAAG TGTGTTTGCA 
     3001 TCACTATGAG AGTGCCTCGG AACCCAACTA TCGGAGATAA ATTTGCCAGT CGCCATGGGC 
     3061 AGAAGGGCAT TTTAAGCAGA TTGTGGCCGG CTGAGGACAT GCCTTTTACT GAGAGTGGGA 
     3121 TGGTCCCAGA CATTCTGTTC AATCCCCATG GTTTTCCATC CCGCATGACC ATTGGGATGT 
     3181 TAATTGAGAG TATGGCCGGG AAGTCTGCAG CTTTGCATGG TCTCTGCCAT GATGCTACAC 
     3241 CCTTCATCTT CTCAGAGGAG AACTCGGCCT TAGAATACTT TGGTGAGATG TTAAAGGCTG 
     3301 CTGGCTACAA TTTCTATGGC ACCGAGAGGT TATATAGTGG CATCAGTGGG CTAGAACTGG 
     3361 AAGCAGACAT CTTCATAGGA GTGGTTTATT ATCAGCGCTT ACGCCATATG GTCTCAGACA 
     3421 AATTTCAAGT AAGGACAACT GGAGCCCGAG ACAGAGTCAC CAACCAGCCT ATTGGGGGAA 
     3481 GAAATGTCCA GGGTGGAATC CGTTTTGGGG AGATGGAACG GGATGCGCTT TTAGCTCATG 
     3541 GTACATCTTT TCTCCTTCAT GACCGCCTCT TCAACTGCTC AGATCGGTCG GTAGCCCATG 
     3601 TGTGTGTGAA GTGTGGCAGT TTACTCTCTC CACTGTTGGA GAAGCCACCC CCTTCTTGGT 
     3661 CTGCCATGCG CAACAGAAAA TACAACTGTA CTCTGTGTAG TCGCAGTGAC ACTATCGATA 
     3721 CTGTTTCTGT GCCTTATGTT TTTCGGTATT TTGTAGCTGA ACTGGCAGCT ATGAACATCA 
     3781 AAGTGAAACT GGATGTTGTT AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3841 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3901 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3961 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4021 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4081 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4141 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4201 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4261 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4321 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4381 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4441 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4501 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4561 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4621 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4681 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4741 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4801 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4861 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4921 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4981 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5041 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5101 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5161 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5221 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5281 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5341 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5401 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5461 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5521 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5581 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5641 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5701 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5761 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5821 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5881 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5941 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6001 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6061 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6121 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6181 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6241 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6301 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6361 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6421 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6481 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6541 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6601 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6661 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6721 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6781 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6841 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6901 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7081 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7141 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7201 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7261 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7321 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7381 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7441 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7501 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7561 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7621 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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