LOCUS       RC239773                7620 bp    DNA     circular  SYN 
DEFINITION  NPHP4 (myc-DDK-tagged) - Human nephronophthisis 4 (NPHP4), transcript variant 2
ACCESSION   NM_001291593
VERSION     NM_001291593.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..3868 
                     /label="Myc-DDK"  
     misc_feature    3898..4483 
                     /label="PolyA signal"  
     misc_feature    3955..3974 
                     /label="Restriction Enzyme"  
     misc_feature    4759..5431 
                     /label="ColE1"  
     misc_feature    5861..6655 
                     /label="Kan/Neo"  
     misc_feature    6690..6992 
                     /label="SV40 ori"  
     misc_feature    7078..7555 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="NPHP4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCCACGG AGCCTGTCAG TGGCCCCAAA GTGGAGCGGC GGCCTTCCAG 
     1081 GAAACCACCC ACGTCCCCTT CGAGAGTTCC CGTTGGAGGC CGGTATCTCC CACCTGGAAG 
     1141 CCGACCTGAG CCAGACCTCC CTGGTCCTGG AAACATCCAT TGCCGAACAG TTACAGGAGC 
     1201 TGCCGTTCAC GCCTTTGCAT GCCCCTATTG TTGTGGGAAC CCAGACCAGG AGCTCTGCAG 
     1261 GGCAGCCCTC GAGAGCCTCC ATGGTGCTCC TGCAGTCCTC CGGCTTTCCC GAGATTCTGG 
     1321 ATGCCAATAA ACAGCCAGCC GAGGCTGTCA GCGCTACAGA ACCTGTGACG TTTAACCCTC 
     1381 AGAAGGAAGA ATCAGATTGT CTACAAAGCA ACGAGATGGT GCTACAGTTT CTTGCCTTTA 
     1441 GCAGAGTGGC CCAGGACTGC CGAGGAACAT CATGGCCAAA GACTGTGTAT TTCACCTTCC 
     1501 AGTTCTACCG CTTCCCACCC GCAACGACGC CACGACTGCA GCTGGTCCAG CTGGATGAGG 
     1561 CCGGCCAGCC CAGCTCTGGC GCCCTGACCC ACATCCTCGT GCCTGTGAGC AGAGATGGCA 
     1621 CCTTTGATGC TGGGTCTCCT GGCTTCCAGC TGAGGTACAT GGTGGGCCCT GGGTTCCTGA 
     1681 AGCCAGGTGA GCGGCGCTGC TTTGCCCGCT ACCTGGCCGT GCAGACCCTG CAGATTGACG 
     1741 TCTGGGACGG AGACTCCCTG CTGCTCATCG GATCTGCTGC CGTCCAGATG AAGCATCTCC 
     1801 TCCGCCAAGG CCGGCCGGCT GTGCAGGCCT CCCACGAGCT TGAGGTCGTG GCAACTGAAT 
     1861 ACGAGCAGGA CAACATGGTG GTGAGTGGAG ACATGCTGGG GTTTGGCCGC GTCAAGCCCA 
     1921 TCGGCGTCCA CTCGGTGGTG AAGGGCCGGC TGCACCTGAC TTTGGCCAAC GTGGGTCACC 
     1981 CGTGTGAACA GAAAGTGAGA GGTTGTAGCA CATTGCCACC GTCCAGATCT CGGGTCATCT 
     2041 CAAACGATGG AGCCAGCCGC TTCTCTGGAG GCAGCCTCCT CACGACTGGA AGCTCAAGGC 
     2101 GAAAACACGT GGTGCAAGCA CAGAAGCTGG CGGACGTGGA CAGTGAGCTG GCTGCCATGC 
     2161 TACTGACCCA TGCCCGGCAG GGCAAGGGGC CCCAGGACGT CAGCCGCGAG TCGGATGCCA 
     2221 CCCGCAGGCG TAAGCTGGAG CGGATGAGGT CTGTGCGCCT GCAGGAGGCC GGGGGAGACT 
     2281 TGGGCCGGCG CGGGACGAGC GTGTTGGCGC AGCAGAGCGT CCGCACACAG CACTTGCGGG 
     2341 ACCTACAGGT CATCGCCGCC TACCGGGAAC GCACGAAGGC CGAGAGCATC GCCAGCCTGC 
     2401 TGAGCCTGGC CATCACCACG GAGCACACGC TCCACGCCAC GCTGGGGGTC GCCGAGTTCT 
     2461 TTGAGTTTGT GCTTAAGAAC CCCCACAACA CACAGCACAC GGTGACTGTG GAGATCGACA 
     2521 ACCCCGAGCT CAGCGTCATC GTGGACAGTC AGGAGTGGAG GGACTTCAAG GGTGCTGCTG 
     2581 GCCTGCACAC ACCGGTGGAG GAGGACATGT TCCACCTGCG TGGCAGCCTG GCCCCCCAGC 
     2641 TCTACCTGCG CCCCCACGAG ACCGCCCACG TCCCCTTCAA GTTCCAGAGC TTCTCTGCAG 
     2701 GGCAGCTGGC CATGGTGCAG GCCTCTCCTG GGTTGAGCAA CGAGAAGGGC ATGGACGCCG 
     2761 TGTCACCTTG GAAGTCCAGC GCAGTGCCCA CTAAACACGC CAAGGTCTTG TTCCGAGCGA 
     2821 GTGGTGGCAA GCCCATCGCC GTGCTCTGCC TGACTGTGGA GCTGCAGCCC CACGTGGTGG 
     2881 ACCAGGTCTT CCGCTTCTAT CACCCGGAGC TCTCCTTCCT GAAGAAGGCC ATCCGCCTGC 
     2941 CGCCCTGGCA CACATTTCCA GGTGCTCCGG TGGGAATGCT TGGTGAGGAC CCCCCAGTCC 
     3001 ATGTTCGCTG CAGCGACCCG AACGTCATCT GTGAGACCCA GAATGTGGGC CCCGGGGAAC 
     3061 CACGGGACAT ATTTCTGAAG GTGGCCAGTG GTCCAAGCCC GGAGATCAAA GACTTCTTTG 
     3121 TCATCATTTA CTCGGATCGC TGGCTGGCGA CACCCACACA GACGTGGCAG GTCTACCTCC 
     3181 ACTCCCTGCA GCGCGTGGAT GTCTCCTGCG TCGCAGGCCA GCTGACCCGC CTGTCCCTTG 
     3241 TCCTTCGGGG GACACAGACA GTGAGGAAAG TGAGAGCTTT CACCTCTCAT CCCCAGGAGC 
     3301 TGAAGACAGA CCCCAAAGGT GTCTTCGTGC TGCCGCCTCG TGGGGTGCAG GACCTGCATG 
     3361 TTGGCGTGAG GCCCCTTAGG GCCGGCAGCC GCTTTGTCCA TCTCAACCTG GTGGACGTGG 
     3421 ATTGCCACCA GCTGGTGGCC TCCTGGCTCG TGTGCCTCTG CTGCCGCCAG CCGCTCATCT 
     3481 CCAAGGCCTT TGAGATCATG TTGGCTGCGG GCGAAGGGAA GGGTGTCAAC AAGAGGATCA 
     3541 CCTACACCAA CCCCTACCCC TCCCGGAGGA CATTCCACCT GCACAGCGAC CACCCGGAGC 
     3601 TGCTGCGGTT CAGAGAGGAC TCCTTCCAGG TCGGGGGTGG AGAGACCTAC ACCATCGGCT 
     3661 TGCAGTTTGC GCCTAGTCAG AGAGTGGGTG AGGAGGAGAT CCTGATCTAC ATCAATGACC 
     3721 ATGAGGACAA AAACGAAGAG GCATTTTGCG TGAAGGTCAT CTACCAGACG CGTACGCGGC 
     3781 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3841 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4561 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5341 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5401 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5461 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5521 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5581 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5641 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5701 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5761 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5821 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5881 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5941 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6001 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6061 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6121 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6181 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6241 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6301 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6361 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6421 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6481 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6541 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6601 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6661 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6721 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6781 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6841 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7021 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7081 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7141 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7201 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7261 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7321 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7381 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7441 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7501 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7561 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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