LOCUS       RC239750                7566 bp    DNA     circular  SYN 
DEFINITION  ZNF281 (myc-DDK-tagged) - Human zinc finger protein 281 (ZNF281), transcript variant 2
ACCESSION   NM_001281293
VERSION     NM_001281293.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3723..3814 
                     /label="Myc-DDK"  
     misc_feature    3844..4429 
                     /label="PolyA signal"  
     misc_feature    3901..3920 
                     /label="Restriction Enzyme"  
     misc_feature    4705..5377 
                     /label="ColE1"  
     misc_feature    5807..6601 
                     /label="Kan/Neo"  
     misc_feature    6636..6938 
                     /label="SV40 ori"  
     misc_feature    7024..7501 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3705..3711 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3705 
                     /label="ZNF281"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATCGGC AGTGGGTTCC TGAGTGGCGG CGGAGGTACC GGCAGTAGCG 
     1081 GTGGTAGCGG CTCCGGCGGC GGTGGTAGTG GCGGCGGCGG CGGCGGCGGC AGCAGCGGCA 
     1141 GGAGGGCAGA GATGGAACCC ACCTTTCCCC AGGGTATGGT TATGTTCAAC CACCGTCTTC 
     1201 CCCCGGTCAC CAGCTTCACC CGGCCGGCGG GGTCGGCCGC CCCTCCCCCG CAATGCGTGT 
     1261 TATCCTCCTC TACCTCCGCA GCCCCGGCCG CTGAGCCCCC CCCTCCGCCA GCCCCGGACA 
     1321 TGACTTTCAA GAAGGAGCCG GCGGCGTCAG CCGCGGCCTT CCCCTCGCAG AGGACCTCCT 
     1381 GGGGGTTCTT GCAGTCTTTG GTTAGCATCA AACAGGAGAA ACCCGCGGAT CCTGAGGAGC 
     1441 AGCAGTCCCA CCACCACCAT CACCACCACC ACTATGGGGG GCTGTTCGCT GGAGCTGAAG 
     1501 AGAGGTCTCC AGGCCTAGGA GGCGGTGAAG GGGGGAGTCA CGGCGTCATC CAGGACCTCA 
     1561 GTATTCTCCA CCAGCATGTC CAGCAGCAAC CAGCCCAGCA CCACCGTGAC GTATTACTCA 
     1621 GCAGCAGTAG CAGGACTGAT GACCACCATG GCACTGAGGA GCCAAAGCAG GACACTAATG 
     1681 TCAAAAAGGC AAAAAGGCCA AAGCCAGAAT CTCAGGGAAT CAAAGCCAAG AGGAAGCCAA 
     1741 GTGCATCTTC CAAACCTTCT TTGGTTGGAG ATGGAGAAGG TGCCATCCTC TCCCCAAGTC 
     1801 AGAAACCTCA TATCTGTGAT CACTGTAGTG CTGCTTTCCG AAGCTCCTAT CACCTGCGGA 
     1861 GACATGTCCT CATTCATACA GGAGAAAGAC CTTTCCAGTG CAGCCAGTGT AGTATGGGTT 
     1921 TCATTCAGAA ATACCTACTA CAGAGACATG AGAAAATTCA TAGTAGAGAG AAGCCATTTG 
     1981 GATGTGATCA GTGCAGCATG AAGTTTATTC AGAAGTACCA TATGGAGAGA CACAAGAGGA 
     2041 CACATAGTGG AGAAAAGCCA TATAAGTGTG ACACTTGCCA ACAGTATTTT TCAAGGACTG 
     2101 ATAGATTGTT GAAGCACAGG CGCACATGTG GTGAAGTCAT AGTTAAAGGA GCCACTAGTG 
     2161 CAGAACCTGG GTCATCAAAC CATACCAATA TGGGTAATCT GGCTGTGTTG TCTCAGGGAA 
     2221 ATACAAGTTC TTCAAGGAGA AAAACAAAGT CAAAAAGCAT AGCTATTGAA AATAAGGAAC 
     2281 AGAAGACCGG TAAAACAAAT GAATCGCAAA TTTCAAATAA TATAAACATG CAGAGTTACT 
     2341 CAGTAGAAAT GCCTACCGTG TCTTCCAGTG GAGGCATAAT TGGCACTGGA ATAGATGAAC 
     2401 TGCAGAAGAG GGTGCCAAAA TTGATCTTTA AGAAAGGAAG CAGAAAGAAT ACAGATAAAA 
     2461 ACTACCTTAA CTTTGTGTCA CCATTACCAG ACATAGTAGG ACAGAAATCC TTGTCTGGAA 
     2521 AACCAAGTGG CTCACTTGGC ATAGTATCAA ATAATAGTGT GGAGACCATT GGTCTTCTCC 
     2581 AAAGTACAAG TGGCAAACAA GGTCAGATAA GTAGTAATTA TGATGATGCC ATGCAGTTTT 
     2641 CAAAGAAAAG AAGATATTTA CCAACTGCCA GCAGCAACAG TGCCTTTTCT ATAAACGTAG 
     2701 GACACATGGT CTCCCAACAG TCTGTCATTC AGTCTGCAGG TGTCAGTGTT TTGGACAATG 
     2761 AGGCACCATT GTCACTTATT GACTCCTCAG CTCTAAATGC TGAAATTAAA TCTTGTCATG 
     2821 ACAAGTCTGG AATTCCTGAT GAGGTTTTAC AAAGTATTTT GGATCAATAC TCCAACAAAT 
     2881 CAGAAAGCCA GAAAGAGGAT CCTTTCAATA TTGCAGAACC ACGAGTGGAT TTACACACCT 
     2941 CAGGAGAACA CTCAGAATTG GTTCAAGAAG AAAATTTGAG CCCAGGCACC CAAACACCTT 
     3001 CAAATGATAA AGCAAGTATG TTGCAAGAAT ACTCCAAATA CCTCCAACAG GCTTTTGAAA 
     3061 AATCCACTAA TGCAAGTTTT ACTCTTGGAC ACGGTTTCCA ATTTGTCAGT TTGTCTTCAC 
     3121 CTCTCCACAA CCACACTTTG TTTCCAGAAA AACAAATATA CACTACGTCT CCTTTGGAGT 
     3181 GTGGTTTCGG CCAATCTGTT ACCTCAGTGT TGCCATCTTC ATTGCCAAAG CCTCCTTTTG 
     3241 GGATGTTGTT TGGATCTCAG CCAGGTCTTT ATTTGTCTGC TTTGGATGCT ACACATCAGC 
     3301 AGTTGACACC TTCCCAGGAG CTGGATGATC TGATAGATTC TCAGAAGAAC TTAGAGACTT 
     3361 CATCAGCCTT CCAGTCCTCA TCTCAGAAAT TGACTAGCCA GAAGGAACAG AAAAACTTAG 
     3421 AGTCTTCAAC AGGCTTTCAG ATTCCATCTC AGGAGTTAGC TAGCCAGATA GATCCTCAGA 
     3481 AAGACATAGA GCCTAGAACA ACGTATCAGA TTGAGAACTT TGCACAAGCG TTTGGTTCTC 
     3541 AGTTTAAGTC GGGCAGCAGG GTGCCAATGA CCTTTATCAC TAACTCTAAT GGAGAAGTGG 
     3601 ACCATAGAGT AAGGACTTCA GTGTCAGATT TCTCAGGGTA TACAAATATG ATGTCTGATG 
     3661 TAAGTGAGCC ATGTAGTACA AGAGTAAAGA CACCCACCAG CCAGAGTTAC AGGACGCGTA 
     3721 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3781 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3841 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3901 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3961 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4021 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4081 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4141 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4201 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4261 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4321 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4381 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4441 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4501 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4561 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4621 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4681 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4741 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4801 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4861 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4921 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4981 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5041 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5101 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5161 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5221 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5281 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5341 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5401 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5461 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5521 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5581 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5641 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5701 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5761 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5821 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5881 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5941 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6001 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6061 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6121 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6181 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6241 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6301 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6361 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6421 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6481 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6541 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6601 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6661 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6721 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6781 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6841 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6901 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6961 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7021 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7081 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7141 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7201 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7261 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7321 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7381 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7441 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7501 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7561 AATTTT
//