LOCUS       RC239748                7563 bp    DNA     circular  SYN 
DEFINITION  ACTN2 (myc-DDK-tagged) - Human actinin, alpha 2 (ACTN2), transcript variant 2
ACCESSION   NM_001278343
VERSION     NM_001278343.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3720..3811 
                     /label="Myc-DDK"  
     misc_feature    3841..4426 
                     /label="PolyA signal"  
     misc_feature    3898..3917 
                     /label="Restriction Enzyme"  
     misc_feature    4702..5374 
                     /label="ColE1"  
     misc_feature    5804..6598 
                     /label="Kan/Neo"  
     misc_feature    6633..6935 
                     /label="SV40 ori"  
     misc_feature    7021..7498 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3702..3708 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3702 
                     /label="ACTN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCAGATA GAGCCCGGCG TGCAGTACAA CTACGTGTAC GACGAGGATG 
     1081 AGTACATGAT CCAGGAGGAG GAGTGGGACC GCGACCTGCT CCTGGACCCA GCCTGGGAGA 
     1141 AGCAGCAGAG GAAGACCTTC ACTGCCTGGT GTAACTCCCA CCTAAGGAAA GCCGGCACCC 
     1201 AGATTGAGAA CATCGAGGAA GACTTCAGGA ATGGCCTTAA GCTCATGCTG CTTTTGGAAG 
     1261 TCATCTCAGG GGAAAGGCTG CCCAAACCTG ACCGGGGAAA AATGCGGTTC CACAAAATTG 
     1321 CTAATGTCAA CAAAGCTTTG GATTACATAG CCAGCAAAGG GGTGAAACTG GTGTCCATCG 
     1381 GCGCTGAAGA AATTGTTGAT GGCAATGTGA AAATGACCCT GGGTATGATC TGGACCATCA 
     1441 TCCTTCGCTT TGCTATTCAG GATATTTCGG TTGAAGAAAC ATCTGCCAAA GAAGGTCTGC 
     1501 TGCTTTGGTG TCAGAGGAAA ACTGCTCCTT ATAGAAATGT GAACATTCAG AACTTCCATA 
     1561 CTAGCTGGAA AGATGGCCTT GGACTCTGTG CCCTCATCCA CCGACACCGG CCTGACCTCA 
     1621 TTGACTACTC AAAGCTTAAC AAGGATGACC CCATAGGAAA TATTAACCTG GCCATGGAAA 
     1681 TCGCTGAGAA GCACCTGGAT ATTCCTAAAA TGTTGGATGC TGAAGATTTA GTATACACTG 
     1741 CCAGACCCGA TGAAAGAGCC ATAATGACTT ATGTTTCCTG TTACTATCAT GCTTTTGCTG 
     1801 GTGCACAGAA GGCCGAGACA GCGGCTAACA GGATATGTAA GGTTCTTGCT GTGAATCAAG 
     1861 AGAATGAGAG GCTGATGGAA GAATATGAGA GGCTAGCGAG TGAGCTTTTG GAATGGATTC 
     1921 GTCGCACGAT CCCCTGGCTG GAGAACCGGA CTCCCGAGAA GACCATGCAA GCCATGCAGA 
     1981 AGAAGCTGGA GGACTTCCGG GATTACCGCC GGAAGCACAA GCCACCCAAG GTGCAGGAGA 
     2041 AATGCCAGCT GGAGATCAAC TTCAACACGC TGCAGACCAA GCTGCGGATC AGCAACCGTC 
     2101 CTGCCTTCAT GCCCTCCGAG GGCAAGATGG TGTCGGATAT TGCTGGTGCC TGGCAGAGGC 
     2161 TGGAGCAGGC TGAGAAGGGT TACGAGGAGT GGTTGCTCAA TGAGATTCGG AGACTGGAGC 
     2221 GCTTGGAACA CCTGGCTGAG AAGTTCAGGC AGAAGGCCTC AACGCACGAG ACTTGGGCTT 
     2281 ATGGCAAAGA GCAGATCTTG CTGCAGAAGG ATTACGAGTC GGCGTCGCTG ACAGAGGTGC 
     2341 GGGCTCTGCT GCGGAAGCAC GAGGCGTTCG AGAGCGACCT GGCAGCGCAC CAGGACCGCG 
     2401 TGGAGCAGAT CGCAGCCATC GCGCAGGAGC TCAATGAACT GGACTATCAC GACGCTGTGA 
     2461 ATGTCAATGA TCGGTGCCAG AAAATTTGTG ACCAGTGGGA CCGACTGGGA ACGCTTACTC 
     2521 AGAAGAGGAG AGAAGCCCTA GAGAGAATGG AGAAATTGCT AGAAACCATT GATCAGCTTC 
     2581 ACCTGGAGTT TGCCAAGAGG GCTGCTCCTT TCAACAATTG GATGGAGGGC GCTATGGAGG 
     2641 ATCTGCAAGA TATGTTCATT GTCCACAGCA TTGAGGAGAT CCAGAGTCTG ATCACTGCGC 
     2701 ATGAGCAGTT CAAGGCCACG CTGCCCGAGG CGGACGGAGA GCGGCAGTCC ATCATGGCCA 
     2761 TCCAGAACGA GGTGGAGAAG GTGATTCAGA GCTACAACAT CAGAATCAGC TCAAGCAACC 
     2821 CGTACAGCAC TGTCACCATG GATGAGCTCC GGACCAAGTG GGACAAGGTG AAGCAACTCG 
     2881 TGCCCATCCG CGATCAATCC CTGCAGGAGG AGCTGGCTCG CCAGCATGCT AACGAGCGTC 
     2941 TGAGGCGCCA GTTTGCTGCC CAAGCCAATG CCATTGGGCC CTGGATCCAG AACAAGATGG 
     3001 AGGAGATTGC CCGGAGCTCC ATCCAGATCA CAGGAGCCCT GGAAGACCAG ATGAACCAGC 
     3061 TGAAGCAGTA TGAGCACAAC ATCATCAACT ATAAGAACAA CATCGACAAG CTGGAGGGAG 
     3121 ACCATCAGCT CATCCAGGAG GCCCTTGTCT TTGACAACAA GCACACGAAC TACACGATGG 
     3181 AGCACATTCG TGTTGGATGG GAGCTGCTGC TGACAACCAT CGCCAGAACC ATCAATGAGG 
     3241 TGGAGACTCA GATCCTGACG AGAGATGCGA AGGGCATCAC CCAGGAGCAG ATGAATGAGT 
     3301 TCAGAGCCTC CTTCAACCAC TTTGACAGGA GGAAGAATGG CCTGATGGAT CATGAGGATT 
     3361 TCAGAGCCTG CCTGATTTCC ATGGGTTATG ACCTGGGTGA AGCCGAATTT GCCCGCATTA 
     3421 TGACCCTGGT AGATCCCAAC GGGCAAGGCA CCGTCACCTT CCAATCCTTC ATCGACTTCA 
     3481 TGACTAGAGA GACGGCTGAC ACCGACACTG CCGAGCAGGT CATCGCCTCC TTCCGGATCC 
     3541 TGGCTTCTGA TAAGCCATAC ATCCTGGCGG AGGAGCTGCG TCGGGAGCTG CCCCCGGATC 
     3601 AGGCCCAGTA CTGCATCAAG AGGATGCCCG CCTACTCGGG CCCAGGCAGT GTGCCTGGTG 
     3661 CACTGGATTA CGCTGCGTTC TCTTCCGCAC TCTACGGGGA GAGCGATCTG ACGCGTACGC 
     3721 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3781 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3841 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3901 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3961 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4021 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4081 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4141 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4201 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4261 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4321 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4381 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4441 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4501 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4561 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4621 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4681 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4741 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4801 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4861 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4921 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4981 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5041 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5101 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5161 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5221 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5281 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5341 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5401 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5461 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5521 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5581 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5641 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5701 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5761 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5821 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5881 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5941 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6001 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6061 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6121 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6181 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6241 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6301 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6361 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6421 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6481 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6541 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6601 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6661 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6721 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6781 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6901 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6961 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7021 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7081 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7141 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7201 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7261 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7321 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7381 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7441 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7501 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7561 TTT
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