LOCUS       RC239741                7548 bp    DNA     circular  SYN 
DEFINITION  ACO1 (myc-DDK-tagged) - Human aconitase 1, soluble (ACO1), transcript variant 1
ACCESSION   NM_001278352
VERSION     NM_001278352.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3705..3796 
                     /label="Myc-DDK"  
     misc_feature    3826..4411 
                     /label="PolyA signal"  
     misc_feature    3883..3902 
                     /label="Restriction Enzyme"  
     misc_feature    4687..5359 
                     /label="ColE1"  
     misc_feature    5789..6583 
                     /label="Kan/Neo"  
     misc_feature    6618..6920 
                     /label="SV40 ori"  
     misc_feature    7006..7483 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3687..3693 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3687 
                     /label="ACO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAACCCA TTCGCACACC TTGCTGAGCC ATTGGATCCT GTACAACCAG 
     1081 GAAAGAAATT CTTCAATTTG AATAAATTGG AGGATTCAAG ATATGGGCGC TTACCATTTT 
     1141 CGATCAGAGT TCTTCTGGAA GCAGCCATTC GGAATTGTGA TGAGTTTTTG GTGAAGAAAC 
     1201 AGGATATTGA AAATATTCTA CATTGGAATG TCACGCAGCA CAAGAACATA GAAGTGCCAT 
     1261 TTAAGCCTGC TCGTGTCATC CTGCAGGACT TTACGGGTGT GCCCGCTGTG GTTGACTTTG 
     1321 CTGCAATGCG TGATGCTGTG AAAAAGTTAG GAGGAGATCC AGAGAAAATA AACCCTGTCT 
     1381 GCCCTGCTGA TCTTGTAATA GATCATTCCA TCCAGGTTGA TTTCAACAGA AGGGCAGACA 
     1441 GTTTACAGAA GAATCAAGAC CTGGAATTTG AAAGAAATAG AGAGCGATTT GAATTTTTAA 
     1501 AGTGGGGTTC CCAGGCTTTT CACAACATGC GGATTATTCC CCCTGGCTCA GGAATCATCC 
     1561 ACCAGGTGAA TTTGGAATAT TTGGCAAGAG TGGTATTTGA TCAGGATGGA TATTATTACC 
     1621 CAGACAGCCT CGTGGGCACA GACTCGCACA CTACCATGAT TGATGGCTTG GGCATTCTTG 
     1681 GTTGGGGTGT CGGTGGTATT GAAGCAGAAG CTGTCATGCT GGGTCAGCCA ATCAGTATGG 
     1741 TGCTTCCTCA GGTGATTGGC TACAGGCTGA TGGGGAAGCC CCACCCTCTG GTAACATCCA 
     1801 CTGACATCGT GCTCACCATT ACCAAGCACC TCCGCCAGGT TGGGGTAGTG GGCAAATTTG 
     1861 TCGAGTTCTT CGGGCCTGGA GTAGCCCAGT TGTCCATTGC TGACCGAGCT ACGATTGCTA 
     1921 ACATGTGTCC AGAGTACGGA GCAACTGCTG CCTTTTTCCC AGTTGATGAA GTTAGTATCA 
     1981 CGTACCTGGT GCAAACAGGT CGTGATGAAG AAAAATTAAA GTATATTAAA AAATATCTTC 
     2041 AGGCTGTAGG AATGTTTCGA GATTTCAATG ACCCTTCTCA AGACCCAGAC TTCACCCAGG 
     2101 TTGTGGAATT AGATTTGAAA ACAGTAGTGC CTTGCTGTAG TGGACCCAAA AGGCCTCAGG 
     2161 ACAAAGTTGC TGTGTCCGAC ATGAAAAAGG ACTTTGAGAG CTGCCTTGGA GCCAAGCAAG 
     2221 GATTTAAAGG ATTCCAAGTT GCTCCTGAAC ATCATAATGA CCATAAGACC TTTATCTATG 
     2281 ATAACACTGA ATTCACCCTT GCTCATGGTT CTGTGGTCAT TGCTGCCATT ACTAGCTGCA 
     2341 CAAACACCAG TAATCCGTCT GTGATGTTAG GGGCAGGATT GTTAGCAAAG AAAGCTGTGG 
     2401 ATGCTGGCCT GAACGTGATG CCTTACATCA AAACTAGCCT GTCTCCTGGG AGTGGCGTGG 
     2461 TCACCTACTA CCTACAAGAA AGCGGAGTCA TGCCTTATCT GTCTCAGCTT GGGTTTGACG 
     2521 TGGTGGGCTA TGGCTGCATG ACCTGCATTG GCAACAGTGG GCCTTTACCT GAACCTGTGG 
     2581 TAGAAGCCAT CACACAGGGA GACCTTGTAG CTGTTGGAGT ACTATCTGGA AACAGGAATT 
     2641 TTGAAGGTCG AGTTCACCCC AACACCCGGG CCAACTATTT AGCCTCTCCC CCCTTAGTAA 
     2701 TAGCATATGC AATTGCTGGA ACCATCAGAA TCGACTTTGA GAAAGAGCCA TTGGGAGTAA 
     2761 ATGCAAAGGG ACAGCAGGTA TTTCTGAAAG ATATCTGGCC GACTAGAGAC GAGATCCAGG 
     2821 CAGTGGAGCG TCAGTATGTC ATCCCGGGGA TGTTTAAGGA AGTCTATCAG AAAATAGAGA 
     2881 CTGTGAATGA AAGCTGGAAT GCCTTAGCAA CCCCATCAGA TAAGCTGTTT TTCTGGAATT 
     2941 CCAAATCTAC GTATATCAAA TCACCACCAT TCTTTGAAAA CCTGACTTTG GATCTTCAGC 
     3001 CCCCTAAATC TATAGTGGAT GCCTATGTGC TGCTAAATTT GGGAGATTCG GTAACAACTG 
     3061 ACCACATCTC CCCAGCTGGA AATATTGCAA GAAACAGTCC TGCTGCTCGC TACTTAACTA 
     3121 ACAGAGGCCT AACTCCACGA GAATTCAACT CCTATGGCTC CCGCCGAGGT AATGACGCCG 
     3181 TCATGGCACG GGGAACATTT GCCAACATTC GCTTGTTAAA CAGATTTTTG AACAAGCAGG 
     3241 CACCACAGAC TATCCATCTG CCTTCTGGGG AAATCCTTGA TGTGTTTGAT GCTGCTGAGC 
     3301 GGTACCAGCA GGCAGGCCTT CCCCTGATCG TTCTGGCTGG CAAAGAGTAC GGTGCAGGCA 
     3361 GCTCCCGAGA CTGGGCAGCT AAGGGCCCTT TCCTGCTGGG AATCAAAGCC GTCCTGGCCG 
     3421 AGAGCTACGA GCGCATTCAC CGCAGTAACC TGGTTGGGAT GGGTGTGATC CCACTTGAAT 
     3481 ATCTCCCTGG TGAGAATGCA GATGCCCTGG GGCTCACAGG GCAAGAACGA TACACTATCA 
     3541 TTATTCCAGA AAACCTCAAA CCACAAATGA AAGTCCAGGT CAAGCTGGAT ACTGGCAAGA 
     3601 CCTTCCAGGC TGTCATGAGG TTTGACACTG ATGTGGAGCT CACTTATTTC CTCAACGGGG 
     3661 GCATCCTCAA CTACATGATC CGCAAGATGG CCAAGACGCG TACGCGGCCG CTCGAGCAGA 
     3721 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3781 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3961 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4021 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4081 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4141 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4201 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4261 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4321 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4381 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4441 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4501 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4561 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4621 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4681 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4741 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4801 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4861 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4921 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4981 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5041 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5101 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5161 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5221 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5281 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5341 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5401 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5461 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5521 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5581 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5641 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5701 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5761 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5821 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5881 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5941 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6001 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6061 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6121 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6181 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6241 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6301 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6361 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6421 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6481 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6541 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6601 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6661 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6721 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6781 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6961 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7021 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7081 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7141 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7201 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7261 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7321 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7381 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7441 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7501 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//