LOCUS       RC239727                7524 bp    DNA     circular  SYN 
DEFINITION  MKL1 (myc-DDK-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1), transcript variant 3
ACCESSION   NM_001282661
VERSION     NM_001282661.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3681..3772 
                     /label="Myc-DDK"  
     misc_feature    3802..4387 
                     /label="PolyA signal"  
     misc_feature    3859..3878 
                     /label="Restriction Enzyme"  
     misc_feature    4663..5335 
                     /label="ColE1"  
     misc_feature    5765..6559 
                     /label="Kan/Neo"  
     misc_feature    6594..6896 
                     /label="SV40 ori"  
     misc_feature    6982..7459 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3663..3669 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3663 
                     /label="MKL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCCTTTG AAAAGTCCAG CCGCATTTCA TGAGCAGAGA AGGAGCTTGG 
     1081 AGCGGGCCAG GACAGAGGAC TATCTCAAAC GGAAGATTCG TTCCCGGCCG GAGAGATCGG 
     1141 AGCTGGTCAG GATGCACATT TTGGAAGAGA CCTCGGCTGA GCCATCCCTC CAGGCCAAGC 
     1201 AGCTGAAGCT GAAGAGAGCC AGACTAGCCG ATGACCTCAA TGAGAAGATT GCACAGAGGC 
     1261 CTGGCCCCAT GGAGCTGGTG GAGAAGAACA TCCTTCCTGT TGAGTCCAGC CTGAAGGAAG 
     1321 CCATCATTGT GGGCCAGGTG AACTATCCCA AAGTAGCAGA CAGCTCTTCC TTCGATGAGG 
     1381 ACAGCAGCGA TGCCTTATCC CCCGAGCAGC CTGCCAGCCA TGAGTCCCAG GGTTCTGTGC 
     1441 CGTCACCCCT GGAGGCCCGA GTCAGCGAAC CACTGCTCAG TGCCACCTCT GCATCCCCCA 
     1501 CCCAGCAAAG CCAACCCAAG TCTGCCAGTG AGAAGTCACA GCGCAGCAAG AAGGCCAAGG 
     1561 AGCTGAAGCC AAAGGTGAAG AAGCTCAAGT ACCACCAGTA CATCCCCCCG GACCAGAAGC 
     1621 AGGACAGGGG GGCACCCCCC ATGGACTCAT CCTACGCCAA GATCCTGCAG CAGCAGCAGC 
     1681 TCTTCCTCCA GCTGCAGATC CTCAACCAGC AGCAGCAGCA GCACCACAAC TACCAGGCCA 
     1741 TCCTGCCTGC CCCGCCAAAG TCAGCAGGCG AGGCCCTGGG AAGCAGCGGG ACCCCCCCAG 
     1801 TACGCAGCCT CTCCACTACC AATAGCAGCT CCAGCTCGGG CGCCCCTGGG CCCTGTGGGC 
     1861 TGGCACGTCA GAACAGCACC TCACTGACTG GCAAGCCGGG AGCCCTGCCG GCCAACCTGG 
     1921 ACGACATGAA GGTGGCAGAG CTGAAGCAGG AGCTGAAGTT GCGATCACTG CCTGTCTCGG 
     1981 GCACCAAAAC TGAGCTGATT GAGCGCCTTC GAGCCTATCA AGACCAAATC AGCCCTGTGC 
     2041 CAGGAGCCCC CAAGGCCCCT GCCGCCACCT CTATCCTGCA CAAGGCTGGC GAGGTGGTGG 
     2101 TAGCCTTCCC AGCGGCCCGG CTGAGCACGG GGCCAGCCCT GGTGGCAGCA GGCCTGGCTC 
     2161 CAGCTGAGGT GGTGGTGGCC ACGGTGGCCA GCAGTGGGGT GGTGAAGTTT GGCAGCACGG 
     2221 GCTCCACGCC CCCCGTGTCT CCCACCCCCT CGGAGCGCTC ACTGCTCAGC ACGGGCGATG 
     2281 AAAACTCCAC CCCCGGGGAC ACCTTTGGTG AGATGGTGAC ATCACCTCTG ACGCAGCTGA 
     2341 CCCTGCAGGC CTCGCCACTG CAGATCCTCG TGAAGGAGGA GGGCCCCCGG GCCGGGTCCT 
     2401 GTTGCCTGAG CCCTGGGGGG CGGGCGGAGC TAGAGGGGCG CGACAAGGAC CAGATGCTGC 
     2461 AGGAGAAAGA CAAGCAGATC GAGGCGCTGA CGCGCATGCT CCGGCAGAAG CAGCAGCTGG 
     2521 TGGAGCGGCT CAAGCTGCAG CTGGAGCAGG AGAAGCGAGC CCAGCAGCCC GCCCCCGCCC 
     2581 CCGCCCCCCT CGGCACCCCC GTGAAGCAGG AGAACAGCTT CTCCAGCTGC CAGCTGAGCC 
     2641 AGCAGCCCCT GGGCCCCGCT CACCCATTCA ACCCCAGCCT GGCGGCCCCA GCCACCAACC 
     2701 ACATAGACCC TTGTGCTGTG GCCCCGGGGC CCCCGTCCGT GGTGGTGAAG CAGGAAGCCT 
     2761 TGCAGCCTGA GCCCGAGCCG GTCCCCGCCC CCCAGTTGCT TCTGGGGCCT CAGGGCCCCA 
     2821 GCCTCATCAA GGGGGTTGCA CCTCCCACCC TCATCACCGA CTCCACAGGG ACCCACCTTG 
     2881 TCCTCACCGT GACCAATAAG AATGCAGACA GCCCTGGCCT GTCCAGTGGG AGCCCCCAGC 
     2941 AGCCCTCGTC CCAGCCTGGC TCTCCAGCGC CTGCCCCCTC TGCCCAGATG GACCTGGAGC 
     3001 ACCCACTGCA GCCCCTCTTT GGGACCCCCA CTTCTCTGCT GAAGAAGGAA CCACCTGGCT 
     3061 ATGAGGAAGC CATGAGCCAG CAGCCCAAAC AGCAGGAAAA TGGTTCCTCA AGCCAGCAGA 
     3121 TGGACGACCT GTTTGACATT CTCATTCAGA GCGGAGAAAT TTCAGCAGAT TTCAAGGAGC 
     3181 CGCCATCCCT GCCAGGGAAG GAGAAGCCAT CCCCGAAGAC AGTCTGTGGG TCCCCCCTGG 
     3241 CAGCACAGCC ATCACCTTCT GCTGAGCTCC CCCAGGCTGC CCCACCTCCT CCAGGCTCAC 
     3301 CCTCCCTCCC TGGACGCCTG GAGGACTTCC TGGAGAGCAG CACGGGGCTG CCCCTGCTGA 
     3361 CCAGTGGGCA TGACGGGCCA GAGCCCCTTT CCCTCATTGA CGACCTCCAT AGCCAGATGC 
     3421 TGAGCAGCAC TGCCATCCTG GACCACCCCC CGTCACCCAT GGACACCTCG GAATTGCACT 
     3481 TTGTTCCTGA GCCCAGCAGC ACCATGGGCC TGGACCTGGC TGATGGCCAC CTGGACAGCA 
     3541 TGGACTGGCT GGAGCTGTCG TCAGGTGGTC CCGTGCTGAG CCTAGCCCCC CTCAGCACCA 
     3601 CAGCCCCCAG CCTCTTCTCC ACAGACTTCC TCGATGGCCA TGATTTGCAG CTGCACTGGG 
     3661 ATTCCTGCTT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3721 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3781 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3841 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3901 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3961 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4021 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4081 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4141 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4201 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4261 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4321 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4381 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4441 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4501 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4561 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4621 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4681 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4741 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4801 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4861 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4921 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4981 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5041 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5101 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5161 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5341 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5401 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5461 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5521 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5581 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5641 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5701 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5761 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5821 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5881 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5941 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6001 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6061 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6121 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6181 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6241 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6301 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6361 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6421 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6481 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6541 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6601 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6661 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6721 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6781 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6841 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6901 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6961 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7021 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7081 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7141 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7201 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7261 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7321 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7381 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7441 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7501 TAACAAAAAT TTAACGCGAA TTTT
//