LOCUS       RC239726                7521 bp    DNA     circular  SYN 
DEFINITION  MAP3K9 (myc-DDK-tagged) - Human mitogen-activated protein kinase kinase kinase 9 (MAP3K9), transcript variant 3
ACCESSION   NM_001284231
VERSION     NM_001284231.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3678..3769 
                     /label="Myc-DDK"  
     misc_feature    3799..4384 
                     /label="PolyA signal"  
     misc_feature    3856..3875 
                     /label="Restriction Enzyme"  
     misc_feature    4660..5332 
                     /label="ColE1"  
     misc_feature    5762..6556 
                     /label="Kan/Neo"  
     misc_feature    6591..6893 
                     /label="SV40 ori"  
     misc_feature    6979..7456 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3660..3666 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3660 
                     /label="MAP3K9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTTACA GGACTGGAAG TTGCCCTGGT ATTGATCCTC CAGAAGGTGG 
     1081 AGAATGGAGA CCTGAGCAAC AAGATTCTGA AGATCACTGA TTTTGGCCTG GCTCGGGAAT 
     1141 GGCACCGAAC CACCAAGATG AGTGCGGCAG GGACGTATGC TTGGATGGCA CCCGAAGTCA 
     1201 TCCGGGCCTC CATGTTTTCC AAAGGCAGTG ATGTGTGGAG CTATGGGGTG CTACTTTGGG 
     1261 AGTTGCTGAC TGGTGAGGTG CCCTTTCGAG GCATTGATGG CTTAGCAGTC GCTTATGGAG 
     1321 TGGCCATGAA CAAACTCGCC CTTCCTATTC CTTCTACGTG CCCAGAACCT TTTGCCAAAC 
     1381 TCATGGAAGA CTGCTGGAAT CCTGATCCCC ACTCACGACC ATCTTTCACG AATATCCTGG 
     1441 ACCAGCTAAC CACCATAGAG GAGTCTGGTT TCTTTGAAAT GCCCAAGGAC TCCTTCCACT 
     1501 GCCTGCAGGA CAACTGGAAA CACGAGATTC AGGAGATGTT TGACCAACTC AGGGCCAAAG 
     1561 AAAAGGAACT TCGCACCTGG GAGGAGGAGC TGACGCGGGC TGCACTGCAG CAGAAGAACC 
     1621 AGGAGGAACT GCTGCGGCGT CGGGAGCAGG AGCTGGCCGA GCGGGAGATT GACATCCTGG 
     1681 AACGGGAGCT CAACATCATC ATCCACCAGC TGTGCCAGGA GAAGCCCCGG GTGAAGAAAC 
     1741 GCAAGGGCAA GTTCAGGAAG AGCCGGCTGA AGCTCAAGGA TGGCAACCGC ATCAGCCTCC 
     1801 CTTCTGATTT CCAGCACAAG TTCACGGTGC AGGCCTCCCC TACCATGGAT AAAAGGAAGA 
     1861 GTCTTATCAA CAGCCGCTCC AGTCCTCCTG CAAGCCCCAC CATCATTCCT CGCCTTCGAG 
     1921 CCATCCAGTG TGAGACTGTT TCCCAAATTA GCTGGGGCCA GAACACACAG GGGCACCTGT 
     1981 CCCCTGCTCT GTCCAGCCAC CGACTGGTCC AGGCTTGCTC CATCCATAAC TTCTGCCACC 
     2041 TCTCCTCGAC AATGTGTATA TACATGCACA TATTGACACC AGGTGAAAGC AGCAAAACCT 
     2101 GGGGCAGGAG CTCAGTCGTC CCAAAGGAGG AAGGGGAGGA GGAGGAGAAG AGGGCCCCAA 
     2161 AGAAGAAGGG ACGGACGTGG GGGCCAGGGA CGCTTGGTCA GAAGGAGCTT GCCTCGGGAG 
     2221 ATGAAGGCCT CAAGTCCCTG GTAGATGGAT ATAAGCAGTG GTCGTCCAGT GCCCCCAACC 
     2281 TGGTGAAGGG CCCAAGGAGT AGCCCGGCCC TGCCAGGGTT CACCAGCCTT ATGGAGATGG 
     2341 CCTTGCTGGC AGCCAGTTGG GTGGTGCCCA TCGACATTGA AGAGGATGAG GACAGTGAAG 
     2401 GCCCAGGGAG TGGAGAGAGT CGCCTACAGC ATTCACCCAG CCAGTCCTAC CTCTGTATCC 
     2461 CATTCCCTCG TGGAGAGGAT GGCGATGGCC CCTCCAGTGA TGGAATCCAT GAGGAGCCCA 
     2521 CCCCAGTCAA CTCGGCCACG AGTACCCCTC AGCTGACGCC AACCAACAGC CTCAAGCGGG 
     2581 GCGGTGCCCA CCACCGCCGC TGCGAGGTGG CTCTGCTCGG CTGTGGGGCT GTTCTGGCAG 
     2641 CCACAGGCCT AGGGTTTGAC TTGCTGGAAG CTGGCAAGTG CCAGCTGCTT CCCCTGGAGG 
     2701 AGCCTGAGCC ACCAGCCCGG GAGGAGAAGA AAAGACGGGA GGGTCTTTTT CAGAGGTCCA 
     2761 GCCGTCCTCG TCGGAGCACC AGCCCCCCAT CCCGAAAGCT TTTCAAGAAG GAGGAGCCCA 
     2821 TGCTGTTGCT AGGAGACCCC TCTGCCTCCC TGACGCTGCT CTCCCTCTCC TCCATCTCCG 
     2881 AGTGCAACTC CACACGCTCC CTGCTGCGCT CCGACAGCGA TGAAATTGTC GTGTATGAGA 
     2941 TGCCAGTCAG CCCAGTCGAG GCCCCTCCCC TGAGTCCATG TACCCACAAC CCCCTGGTCA 
     3001 ATGTCCGAGT AGAGCGCTTC AAACGAGATC CTAACCAATC TCTGACTCCC ACCCATGTCA 
     3061 CCCTCACCAC CCCCTCGCAG CCCAGCAGTC ACCGGCGGAC TCCTTCTGAT GGGGCCCTTA 
     3121 AGCCAGAGAC TCTCCTAGCC AGCAGGAGCC CCTCCAGCAA TGGGTTGAGC CCCAGTCCTG 
     3181 GAGCAGGAAT GTTGAAAACC CCCAGTCCCA GCCGAGACCC AGGTGAATTC CCCCGTCTCC 
     3241 CTGACCCCAA TGTGGTCTTC CCCCCAACCC CAAGGCGCTG GAACACTCAG CAGGACTCTA 
     3301 CCTTGGAGAG ACCCAAGACT CTGGAGTTTC TGCCTCGGCC GCGTCCTTCT GCCAACCGGC 
     3361 AACGGCTGGA CCCTTGGTGG TTTGTGTCCC CCAGCCATGC CCGCAGCACC TCCCCAGCCA 
     3421 ACAGCTCCAG CACAGAGACG CCCAGCAACC TGGACTCCTG CTTTGCTAGC AGTAGCAGCA 
     3481 CTGTAGAGGA GCGGCCTGGA CTTCCAGCCC TGCTCCCGTT CCAGGCAGGG CCGCTGCCCC 
     3541 CGACTGAGCG GACGCTCCTG GACCTGGATG CAGAGGGGCA GAGTCAGGAC AGCACCGTGC 
     3601 CGCTGTGCAG AGCGGAACTG AACACACACA GGCCTGCCCC TTATGAGATC CAGCAGGAGT 
     3661 TCTGGTCTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3721 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3781 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3841 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3901 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3961 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4021 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4081 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4141 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4201 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4261 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4321 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4381 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4441 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4501 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4561 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4621 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4681 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4741 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4801 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4861 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4921 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4981 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5041 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5101 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5161 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5221 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5281 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5341 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5401 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5461 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5521 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5581 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5641 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5701 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5761 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5821 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5881 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5941 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6001 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6061 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6121 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6181 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6241 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6301 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6361 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6421 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6481 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6541 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6601 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6661 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6721 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6781 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6841 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6961 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7021 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7081 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7141 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7201 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7261 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7321 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7381 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7441 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7501 CAAAAATTTA ACGCGAATTT T
//