LOCUS       RC239725                7521 bp    DNA     circular  SYN 
DEFINITION  MCM8 (myc-DDK-tagged) - Human minichromosome maintenance complex component 8 (MCM8), transcript variant 4
ACCESSION   NM_001281521
VERSION     NM_001281521.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3678..3769 
                     /label="Myc-DDK"  
     misc_feature    3799..4384 
                     /label="PolyA signal"  
     misc_feature    3856..3875 
                     /label="Restriction Enzyme"  
     misc_feature    4660..5332 
                     /label="ColE1"  
     misc_feature    5762..6556 
                     /label="Kan/Neo"  
     misc_feature    6591..6893 
                     /label="SV40 ori"  
     misc_feature    6979..7456 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3660..3666 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3660 
                     /label="MCM8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATGGAGAG TATAGAGGCA GAGGATTTGG ACGAGGAAGA TTTCAAAGCT 
     1081 GGAAAAGGGG AAGAGGTGGT GGGAACTTCT CAGGAAAATG GAGAGAAAGA GAACACAGAC 
     1141 CTGATCTGAG TAAAACCACA GGAAAACGTA CTTCTGAACA AACCCCACAG TTTTTGCTTT 
     1201 CAACAAAGAC CCCACAGTCA ATGCAGTCAA CATTGGATCG ATTCATACCA TATAAAGGCT 
     1261 GGAAGCTTTA TTTCTCTGAA GTTTACAGCG ATAGCTCTCC TTTGATTGAG AAGATTCAAG 
     1321 CATTTGAAAA ATTTTTCACA AGGCATATTG ATTTGTATGA CAAGGATGAA ATAGAAAGAA 
     1381 AGGGAAGTAT TTTGGTAGAT TTTAAAGAAC TGACAGAAGG TGGTGAAGTA ACTAACTTGA 
     1441 TACCAGATAT AGCAACTGAA CTAAGAGATG CACCTGAGAA AACCTTGGCT TGCATGGGTT 
     1501 TGGCAATACA TCAGGTGTTA ACTAAGGACC TTGAAAGGCA TGCAGCTGAG TTACAAGCCC 
     1561 AGGAAGGATT GTCTAATGAT GGAGAAACAA TGGTAAATGT GCCACATATT CATGCAAGGG 
     1621 TGTACAACTA TGAGCCTTTG ACACAGCTCA AGAATGTCAG AGCAAATTAC TATGGAAAAT 
     1681 ACATTGCTCT AAGAGGGACA GTGGTTCGTG TCAGTAATAT AAAGCCTCTT TGCACCAAGA 
     1741 TGGCTTTTCT TTGTGCTGCA TGTGGAGAAA TTCAGAGCTT TCCTCTTCCA GATGGAAAAT 
     1801 ACAGTCTTCC CACAAAGTGT CCTGTGCCTG TGTGTCGAGG CAGGTCATTT ACTGCTCTCC 
     1861 GCAGCTCTCC TCTCACAGTT ACGATGGACT GGCAGTCAAT CAAAATCCAG GAATTGATGT 
     1921 CTGATGATCA GAGAGAAGCA GGTCGGATTC CACGAACAAT AGAATGTGAG CTTGTTCATG 
     1981 ATCTTGTGGA TAGCTGTGTC CCGGGAGACA CAGTGACTAT TACTGGAATT GTCAAAGTCT 
     2041 CAAATGCGGA AGAAGGTTCT CGAAATAAGA ATGACAAGTG TATGTTCCTT TTGTATATTG 
     2101 AAGCAAATTC TATTAGTAAT AGCAAAGGAC AGAAAACAAA GAGTTCTGAG GATGGGTGTA 
     2161 AGCATGGAAT GTTGATGGAG TTCTCACTTA AAGACCTTTA TGCCATCCAA GAGATTCAAG 
     2221 CTGAAGAAAA CCTGTTTAAA CTCATTGTCA AATGGAGTCT TGCTCTGTCG CCCAGGCTAG 
     2281 AGTACAGTGG TGCGATCTCA GCTCACTGCA ACCTCCACCT CCCAAGTTCA AACAGTTCTC 
     2341 CCACCTCAGC CTGCCGAGTA GCTGGGACTA CAGGCATGCG CCACCAGACC CAGCTACTTT 
     2401 TGCTTGTTAA AGCAGGTTTG GCATTAGCAC TCTTTGGAGG AAGCCAGAAA TACGCAGATG 
     2461 ACAAAAACAG AATTCCAATT CGGGGAGACC CCCACATCCT TGTTGTTGGA GATCCAGGCC 
     2521 TAGGAAAAAG TCAAATGCTA CAGGCAGCGT GCAATGTTGC CCCACGTGGC GTGTATGTTT 
     2581 GTGGTAACAC CACGACCACC TCTGGTCTGA CGGTAACTCT TTCAAAAGAT AGTTCCTCTG 
     2641 GAGATTTTGC TTTGGAAGCT GGTGCCCTGG TACTTGGTGA TCAAGGTATT TGTGGAATCG 
     2701 ATGAATTTGA TAAGATGGGG AATCAACATC AAGCCTTGTT GGAAGCCATG GAGCAGCAAA 
     2761 GTATTAGTCT TGCTAAGGCT GGTGTGGTTT GTAGCCTTCC TGCAAGAACT TCCATTATTG 
     2821 CTGCTGCAAA TCCAGTTGGA GGACATTACA ATAAAGCCAA AACAGTTTCT GAGAATTTAA 
     2881 AAATGGGGAG TGCACTACTA TCCAGATTTG ATTTGGTCTT TATCCTGTTA GATACTCCAA 
     2941 ATGAGCATCA TGATCACTTA CTCTCTGAAC ATGTGATTGC AATAAGAGCT GGAAAGCAGA 
     3001 GAACCATTAG CAGTGCCACA GTAGCTCGTA TGAATAGTCA AGATTCAAAT ACTTCCGTAC 
     3061 TTGAAGTAGT TTCTGAGAAG CCATTATCAG AAAGACTAAA GGTGGTTCCT GGAGAAACAA 
     3121 TAGATCCCAT TCCCCACCAG CTATTGAGAA AGTACATTGG CTATGCTCGG CAGTATGTGT 
     3181 ACCCAAGGCT ATCCACAGAA GCTGCTCGAG TTCTTCAAGA TTTTTACCTT GAGCTCCGGA 
     3241 AACAGAGCCA GAGGTTAAAT AGCTCACCAA TCACTACCAG GCAGCTGGAA TCTTTGATTC 
     3301 GTCTGACAGA GGCACGAGCA AGGTTGGAAT TGAGAGAGGA AGCAACCAAA GAAGACGCTG 
     3361 AGGATATAGT GGAAATTATG AAATATAGCA TGCTAGGAAC TTACTCTGAT GAATTTGGGA 
     3421 ACCTAGATTT TGAGCGATCC CAGCATGGTT CTGGAATGAG CAACAGGTCA ACAGCGAAAA 
     3481 GATTTATTTC TGCTCTCAAC AACGTTGCTG AAAGAACTTA TAATAATATA TTTCAATTTC 
     3541 ATCAACTTCG GCAGATTGCC AAAGAACTAA ACATTCAGGT TGCTGATTTT GAAAATTTTA 
     3601 TTGGATCACT AAATGACCAG GGTTACCTCT TGAAAAAAGG CCCAAAAGTT TACCAGCTTC 
     3661 AAACTATGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3721 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3781 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3841 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3901 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3961 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4021 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4081 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4141 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4201 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4261 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4321 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4381 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4441 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4501 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4561 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4621 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4681 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4741 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4801 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4861 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4921 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4981 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5041 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5101 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5161 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5221 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5281 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5341 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5401 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5461 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5521 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5581 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5641 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5701 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5761 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5821 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5881 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5941 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6001 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6061 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6121 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6181 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6241 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6301 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6361 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6421 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6481 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6541 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6601 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6661 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6721 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6781 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6841 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6961 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7021 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7081 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7141 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7201 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7261 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7321 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7381 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7441 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7501 CAAAAATTTA ACGCGAATTT T
//