LOCUS       RC239715                7497 bp    DNA     circular  SYN 
DEFINITION  DNAH2 (myc-DDK-tagged) - Human dynein, axonemal, heavy chain 2 (DNAH2), transcript variant 2
ACCESSION   NM_001303270
VERSION     NM_001303270.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3654..3745 
                     /label="Myc-DDK"  
     misc_feature    3775..4360 
                     /label="PolyA signal"  
     misc_feature    3832..3851 
                     /label="Restriction Enzyme"  
     misc_feature    4636..5308 
                     /label="ColE1"  
     misc_feature    5738..6532 
                     /label="Kan/Neo"  
     misc_feature    6567..6869 
                     /label="SV40 ori"  
     misc_feature    6955..7432 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3636..3642 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3636 
                     /label="DNAH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAGCAAA GCTGAGAAGA AGCAGCGATT GAGTGGCCGA GGAAGCTCCC 
     1081 AGGCAAGCTG GTCAGGGCGG GCCACTCGGG CTGCTGTGGC CACACAGGAG CAGGGGAATG 
     1141 CCCCGGCTGT CAGTGAGCCA GAGCTGCAGG CTGAGCTCCC CAAGGAGGAG CCTGAGCCAC 
     1201 GGTTGGAGGG ACCTCAAGCA CAGAGTGAAG AATCAGTGGA GCCCGAGGCA GATGTGAAGC 
     1261 CCCTCTTCCT TTCCCGAGCT GCGCTGACAG GACTGGCGGA TGCAGTGTGG ACACAGGAGC 
     1321 ATGATGCCAT TCTGGAACAC TTTGCCCAGG ACCCTACAGA ATCCATCCTC ACCATCTTCA 
     1381 TTGACCCTTG TTTTGGGCTG AAGCTAGAGC TGGGCATGCC TGTACAGACC CAGAACCAGC 
     1441 TTGTCTACTT CATTCGCCAA GCACCAGTTC CCATCACCTG GGAGAACTTC GAGGCAACTG 
     1501 TGCAGTTTGG GACGGTGCGG GGCCCCTATA TCCCGGCCCT GCTTCGGCTG CTCGGTGGAG 
     1561 TCTTTGCCCC TCAGATCTTT GCAAACACAG GCTGGCCTGA GAGCATTAGA AATCATTTTG 
     1621 CTTCTCATCT GCACAAGTTC TTGGCCTGCC TGACAGACAC TCGGTACAAA CTGGAGGGGC 
     1681 ACACGGTCCT CTACATCCCT GCAGAGGCCA TGAACATGAA GCCTGAGATG GTGATAAAGG 
     1741 ACAAAGAGCT GGTGCAACGG CTAGAGACCT CCATGATCCA CTGGACCCGG CAGATAAAGG 
     1801 AGATGCTCAG TGCCCAGGAG ACTGTGGAGA CAGGAGAAAA TTTAGGTCCT CTGGAGGAGA 
     1861 TTGAGTTCTG GCGCAACCGA TGCATGGACC TGTCTGGCAT CAGTAAGCAG CTGGTGAAGA 
     1921 AGGGAGTGAA GCACGTTGAA TCCATCCTGC ACCTTGCCAA GTCGTCCTAC TTGGCGCCCT 
     1981 TTATGAAACT GGCACAGCAG ATCCAGGATG GCTCTCGTCA AGCACAGTCA AACCTGACCT 
     2041 TTTTGTCAAT CCTGAAGGAA CCTTACCAGG AGTTGGCTTT CATGAAGCCC AAGGACATCT 
     2101 CTAGCAAGCT CCCTAAGCTG ATCAGTCTCA TCCGCATCAT CTGGGTCAAC TCTCCCCACT 
     2161 ACAACACTCG GGAGAGACTG ACCTCGCTCT TCCGAAAGAT GAGCAATGAG ATCATCCGCT 
     2221 TATGCTGCCA CGCCATCTCC CTGGACCGGA TCTTTGAGGG ATATGTCTCT TCCAGCAAGG 
     2281 AGGACCTGCA AGGCTGCATT CTCTGTTGTC ACGCTTGGAA AGATCACTAC GTACAGGCTG 
     2341 TGCAGATGCA CATCCAGTTC TCCAGTCGGG GCTGGGTCCT AGATCAGACC AGCATCTTTG 
     2401 CTCAGGTTGA TGCCTTTGTG CAGCGCTGCA AGGACCTTAT TGAGGTATGT GACTGTCAGT 
     2461 ATCACTTCGC CCGCTGGGAA GATGGCAAGC AGGGTCCCCT TCCTTGCTTC TTTGGTGCCC 
     2521 AGGGGCCACA GATAACACGG AACTTGCTGG AGATTGAGGA CATCTTTCAT AAAAATCTGC 
     2581 ACACGCTGCG AGCCGTTCGC GGGGGTATCC TGGATGTCAA GAACACCTGT TGGCATGAAG 
     2641 ACTACAATAA GTTCCGTGCC GGAATCAAGG ACCTGGAGGT GATGACCCAG AACCTGATCA 
     2701 CCTCAGCCTT CGAGTTGGTG CGGGACGTGC CGCACGGCGT GCTTCTGCTG GACACCTTCC 
     2761 ACAGGCTTGC CTCCCGCGAG GCTATCAAGC GGACTTATGA CAAGAAGGCG GTGGATCTCT 
     2821 ACATGCTGTT CAATAGCGAG CTGGCCCTGG TGAACCGTGA ACGGAACAAG AAATGGCCAG 
     2881 ACCTGGAGCC CTACGTGGCC CAGTATTCCG GAAAGGCGCG CTGGGTGCAC ATCCTCCGGC 
     2941 GTCGCATCGA CAGAGTCATG ACCTGCCTTG CTGGTGCTCA TTTCCTGCCC CGTATTGGGA 
     3001 CTGGAAAGGA GAGTGTGCAC ACCTATCAGC AGATGGTCCA GGCCATTGAT GAGCTGGTTC 
     3061 GAAAAACCTT CCAAGAGTGG ACATCAAGTC TGGACAAGGA TTGCATTCGG CGGTTGGATA 
     3121 CCCCATTGCT GCGAATCAGC CAGGAGAAGG CGGGCATGCT GGATGTCAAC TTTGACAAGT 
     3181 ACAGGAGCCA CCTGGCCCCT TTTCCCTATA CTCCCCTTCT GCAGCTCTCC CAAGAATTTC 
     3241 ACTCCCATCT TTTGACTCCT CTCTTCATTA TTCTCTCTCT TTCTCATACA ATATGTTTGC 
     3301 TATCGTCATT TTACTTTTTT TTTTCTTCTT TTATTTTTGT CTCTCCCCAC CTCCCACCCT 
     3361 GCTATCAACA TTTTAATTTT ACAACTTATC TTAAAACACA ACAAAACAAA ACAATGATCG 
     3421 GCCAGGCACG GTGGCTCACA CCTGTAATCC CAGCACTTTG GGAGGCTGAG GTGGGCGCAT 
     3481 CGCTTGAGCC CAGGAGTTTG AGAACAGCCT GGGCAACATG GCAAAACCCA GTCTCTGCAA 
     3541 AAAATACAAA GATTAGCTGG GCGTGGTGGC ACAAGCCTGT AGTCTCAGCT ACTTGGGAGG 
     3601 GTGAGGTGGG AGGATCGCCT GAGCCCGGGA GGCAGAGGCT GCAGACGCGT ACGCGGCCGC 
     3661 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3721 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3781 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3841 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3901 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3961 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4021 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4081 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4141 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4201 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4261 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4321 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4381 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4441 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4501 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4561 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4621 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4681 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4741 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4801 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4861 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4921 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4981 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5041 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5101 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5161 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5221 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5281 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5341 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5401 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5461 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5521 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5581 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5641 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5701 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5761 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5821 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5881 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5941 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6001 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6061 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6121 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6181 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6241 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6301 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6361 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6421 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6481 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6541 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6601 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6661 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6721 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6781 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6841 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6901 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6961 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7021 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7081 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7141 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7201 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7261 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7321 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7381 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7441 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//