LOCUS       RC239676                7431 bp    DNA     circular  SYN 
DEFINITION  PIGG (myc-DDK-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 4
ACCESSION   NM_001289052
VERSION     NM_001289052.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3588..3679 
                     /label="Myc-DDK"  
     misc_feature    3709..4294 
                     /label="PolyA signal"  
     misc_feature    3766..3785 
                     /label="Restriction Enzyme"  
     misc_feature    4570..5242 
                     /label="ColE1"  
     misc_feature    5672..6466 
                     /label="Kan/Neo"  
     misc_feature    6501..6803 
                     /label="SV40 ori"  
     misc_feature    6889..7366 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3570..3576 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3570 
                     /label="PIGG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCTGGGC TCCGGGACTT TCGCTACCTG TTGCGTAGCG ATCGAGGTGC 
     1081 TAGGGATCGC GGTCTTCCTT CGGGGATTCT TCCCGGCTCC CGTTCGTTCC TCTGCCAGAG 
     1141 CGGAACACGG AGCGGAGCCC CCAGCGCCCG AACCCTCGGC TGGAGCCAGT TCTAACTGGA 
     1201 CCACGCTGCC ACCACCTCTC TTCAGTAAAG TTGTTATTGT TCTGATAGAT GCCTTGAGAG 
     1261 ATGATTTTGT GTTTGGGTCA AAGGGTGTGA AATTTATGCC CTACACAACT TACCTTGTGG 
     1321 AAAAAGGAGC ATCTCACAGT TTTGTGGCTG AAGCAAAGCC ACCTACAGTT ACTATGCCTC 
     1381 GAATCAAGGA GAGAGAGACG CCTTTACCCA ATTTGCTGGT TCTTTGTGGT GACCATGGCA 
     1441 TGTCTGAAAC AGGAAGTCAC GGGGCCTCCT CCACCGAGGA GGTGAATACA CCTCTGATTT 
     1501 TAATCAGTTC TGCGTTTGAA AGGAAACCCG GTGATATCCG ACATCCAAAG CACGTCCAAC 
     1561 AGACGGATGT GGCTGCGACA CTGGCGATAG CACTTGGCTT ACCGATTCCA AAAGACAGTG 
     1621 TAGGGAGCCT CCTATTCCCA GTTGTGGAAG GAAGACCAAT GAGAGAGCAG TTGAGATTTT 
     1681 TACATTTGAA TACAGTGCAG CTTAGTAAAC TGTTGCAAGA GAATGTGCCG TCATATGAAA 
     1741 AAGATCCTGG GTTTGAGCAG TTTAAAATGT CAGAAAGATT GCATGGGAAC TGGATCAGAC 
     1801 TGTACTTGGA GGAAAAGCAT TCAGAAGTCC TATTCAACCT GGGCTCCAAG GTTCTCAGGC 
     1861 AGTACCTGGA TGCTCTGAAG ACGCTGAGCT TGTCCCTGAG TGCACAAGTG GCCCAGTACG 
     1921 ACATCTATTC GATGATGGTG GGGACTGTCG TGGTTTTGGA GGTTCTCACC CTGCTCCTGC 
     1981 TCAGCGTCCC ACAGGCACTG CGCAGAAAGG CTGAGCTGGA AGTCCCACTG TCATCTCCTG 
     2041 GGTTTTCTCT GCTCTTTTAT TTGGTGATCC TGGTTCTTTC GGCCGTTCAC GTCATTGTGT 
     2101 GCACCTCAGC TGAAAGTTCG TGCTACTTCT GTGGCCTCTC GTGGCTGGCG GCAGGTGGGG 
     2161 TGATGGTGCT GGCCTCGGCG CTGCTGTGTG TGATTGTGTC TGTTCTGACC AACGTGCTCG 
     2221 TGGGTGGAAA CACCCCAAGG AAGAACCCCA TGCATCCCAG CTCAAGGTGG TCAGAGCTAG 
     2281 ACCTTCTTAT TCTGTTGGGG ACGGCGGGCC ACGTCTTGAG CCTGGGCGCC AGCAGCTTCG 
     2341 TGGAGGAGGA GCACCAGACC TGGTACTTCC TTGTGAACAC CCTGTGTCTA GCTCTGAGCC 
     2401 AAGAAACCTA CAGAAACTAC TTTCTGGGAG ATGACGGTGA GCCTCCGTGT GGCCTCTGTG 
     2461 TGGAACAAGG GCATGACGGG GCCACAGCAG CGTGGCAGGA CGGGCCTGGC TGTGATGTCC 
     2521 TGGAGCGAGA CAAAGGCCAC GGAAGCCCCT CTACCTCCGA AGTGCTCAGA GGCCGCGAGA 
     2581 AGTGGATGGT GCTGGCCAGT CCGTGGCTAA TACTGGCCTG CTGCCGGCTG CTGCGCTCCC 
     2641 TAAACCAGAC AGGTGTGCAG TGGGCTCACC GGCCTGACCT CGGCCACTGG CTCACCAGCT 
     2701 CTGACCACAA AGCCGAGCTC TCTGTCCTGG CTGCCCTCTC CCTCCTCGTA GTTTTTGTGC 
     2761 TGGTGCAGAG GGGGTGCTCC CCTGTGTCCA AGGCTGCCCT GGCGCTGGGG CTGCTGGGCG 
     2821 TCTACTGCTA CCGGGCGGCC ATCGGGAGTG TCCGGTTCCC GTGGCGGCCG GACAGCAAGG 
     2881 ACATTTCCAA GGGTATTATT GAAGCTCGTT TTGTTTATGT CTTTGTCCTT GGCATTCTGT 
     2941 TCACGGGCAC CAAAGACTTA CTTAAATCTC AAGTCATTGC TGCAGACTTC AAACTCAAGA 
     3001 CTGTAGGTTT ATGGGAGATA TATAGTGGAT TAGTTCTTCT GGCAGCCTTG CTCTTTAGAC 
     3061 CACATAATCT TCCGGTCTTA GCATTTAGCC TCTTGATTCA GACTCTAATG ACTAAATTCA 
     3121 TCTGGAAGCC CCTGAGACAC GATGCAGCTG AGATTACTGT GATGCATTAT TGGTTTGGTC 
     3181 AAGCATTCTT CTATTTTCAG GGCAACTCCA ACAACATTGC CACCGTGGAC ATCTCCGCAG 
     3241 GCTTCGTGGG CTTAGACACC TACGTGGAAA TCCCAGCCGT GCTCCTGACA GCGTTTGGGA 
     3301 CGTACGCAGG GCCTGTGCTG TGGGCCAGCC ACTTAGTGCA CTTCCTGAGC TCAGAAACAC 
     3361 GCAGTGGTTC AGCACTGAGT CATGCTTGCT TCTGCTACGC ACTGATTTGT TCTATTCCAG 
     3421 TTTTCACGTA CATCGTTTTG GTGACATCTC TGCGTTATCA TTTATTTATA TGGAGTGTAT 
     3481 TTTCTCCAAA ACTTCTCTAC GAGGGAATGC ACCTGCTCAT TACAGCTGCT GTCTGTGTAT 
     3541 TCTTCACGGC AATGGATCAA ACCAGACTCA CACAGTCTAC GCGTACGCGG CCGCTCGAGC 
     3601 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3661 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3721 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3781 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3841 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3901 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3961 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4021 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4081 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4141 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4201 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4261 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4321 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4381 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4441 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4501 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4561 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4621 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4681 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4741 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4801 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4861 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4921 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4981 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5041 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5101 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5161 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5221 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5281 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5341 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5401 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5461 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5521 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5581 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5641 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5701 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5761 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5821 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5881 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5941 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6001 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6061 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6121 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6181 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6241 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6301 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6361 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6421 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6481 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6541 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6601 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6661 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6721 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6781 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6841 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6901 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6961 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7021 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7081 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7141 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7201 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7261 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7321 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7381 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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