LOCUS       RC239573                7254 bp    DNA     circular  SYN 
DEFINITION  STIM1 (myc-DDK-tagged) - Human stromal interaction molecule 1 (STIM1), transcript variant 1
ACCESSION   NM_001277961
VERSION     NM_001277961.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3411..3502 
                     /label="Myc-DDK"  
     misc_feature    3532..4117 
                     /label="PolyA signal"  
     misc_feature    3589..3608 
                     /label="Restriction Enzyme"  
     misc_feature    4393..5065 
                     /label="ColE1"  
     misc_feature    5495..6289 
                     /label="Kan/Neo"  
     misc_feature    6324..6626 
                     /label="SV40 ori"  
     misc_feature    6712..7189 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3393..3399 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3393 
                     /label="STIM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTATGC GTCCGTCTTG CCCTGTGGCT CCTCTGGGGA CTCCTCCTGC 
     1081 ACCAGGGCCA GAGCCTCAGC CATAGTCACA GTGAGAAGGC GACAGGAACC AGCTCGGGGG 
     1141 CCAACTCTGA GGAGTCCACT GCAGCAGAGT TTTGCCGAAT TGACAAGCCC CTGTGTCACA 
     1201 GTGAGGATGA GAAACTCAGC TTCGAGGCAG TCCGTAACAT CCACAAACTG ATGGACGATG 
     1261 ATGCCAATGG TGATGTGGAT GTGGAAGAAA GTGATGAGTT CCTGAGGGAA GACCTCAATT 
     1321 ACCATGACCC AACAGTGAAA CACAGCACCT TCCATGGTGA GGATAAGCTC ATCAGCGTGG 
     1381 AGGACCTGTG GAAGGCATGG AAGTCATCAG AAGTATACAA TTGGACCGTG GATGAGGTGG 
     1441 TACAGTGGCT GATCACATAT GTGGAGCTGC CTCAGTATGA GGAGACCTTC CGGAAGCTGC 
     1501 AGCTCAGTGG CCATGCCATG CCAAGGCTGG CTGTCACCAA CACCACCATG ACAGGGACTG 
     1561 TGCTGAAGAT GACAGACCGG AGTCATCGGC AGAAGCTGCA GCTGAAGGCT CTGGATACAG 
     1621 TGCTCTTTGG GCCTCCTCTC TTGACTCGCC ATAATCACCT CAAGGACTTC ATGCTGGTGG 
     1681 TGTCTATCGT TATTGGTGTG GGCGGCTGCT GGTTTGCCTA TATCCAGAAC CGTTACTCCA 
     1741 AGGAGCACAT GAAGAAGATG ATGAAGGACT TGGAGGGGTT ACACCGAGCT GAGCAGAGTC 
     1801 TGCATGACCT TCAGGAAAGG CTGCACAAGG CCCAGGAGGA GCACCGCACA GTGGAGGTGG 
     1861 AGAAGGTCCA TCTGGAAAAG AAGCTGCGCG ATGAGATCAA CCTTGCTAAG CAGGAAGCCC 
     1921 AGCGGCTGAA GGAGCTGCGG GAGGGTACTG AGAATGAGCG GAGCCGCCAA AAATATGCTG 
     1981 AGGAGGAGTT GGAGCAGGTT CGGGAGGCCT TGAGGAAAGC AGAGAAGGAG CTAGAATCTC 
     2041 ACAGCTCATG GTATGCTCCA GAGGCCCTTC AGAAGTGGCT GCAGCTGACA CATGAGGTGG 
     2101 AGGTGCAATA TTACAACATC AAGAAGCAAA ATGCTGAGAA GCAGCTGCTG GTGGCCAAGG 
     2161 AGGGGGCTGA GAAGATAAAA AAGAAGAGAA ACACACTCTT TGGCACCTTC CACGTGGCCC 
     2221 ACAGCTCTTC CCTGGATGAT GTAGATCATA AAATTCTAAC AGCTAAGCAA GCACTGAGCG 
     2281 AGGTGACAGC AGCATTGCGG GAGCGCCTGC ACCGCTGGCA ACAGATCGAG ATCCTCTGTG 
     2341 GCTTCCAGAT TGTCAACAAC CCTGGCATCC ACTCACTGGT GGCTGCCCTC AACATAGACC 
     2401 CCAGCTGGAT GGGCAGTACA CGCCCCAACC CTGCTCACTT CATCATGACT GACGACGTGG 
     2461 ATGACATGGA TGAGGAGATT GTGTCTCCCT TGTCCATGCA GTCCCCTAGC CTGCAGAGCA 
     2521 GTGTTCGGCA GCGCCTGACG GAGCCACAGC ATGGCCTGGG ATCTCAGAGG TTGGTAGAGG 
     2581 GCGAGGCTGG CCACTTCTTG ACAAGCCGGG TATCTCTGCG GCGAATGCGC AGCCTTTCAT 
     2641 CTGGACAGTC TTTCAGTTCT GAAGGCTACG GGACCAGCTC TCCATCTGCC TCTGCTGCTG 
     2701 CTTCTTGCTC CTCTTCCATC ACCACCATCA CCACTACCAC CACCACCACC ACCACCTTCA 
     2761 CCACCGTCCA TGTCCACCCT GTTTATTACC ACCACAGCAC TTCCTATTTC CTCCAGATGG 
     2821 AGCCCTACCC TGACACACCC CCTTCTGACA GCACCGCTGT GATGCCTGGG CATTCAGAGA 
     2881 GCTTGGGGGA TTTGACCCAT TCCGATTCGG AGTCCTCCCT CCACATGAGT GACCGCCAGC 
     2941 GTGTGGCCCC CAAACCTCCT CAGATGAGCC GTGCTGCAGA CGAGGCTCTC AATGCCATGA 
     3001 CTTCCAATGG CAGCCACCGG CTGATCGAGG GGGTCCACCC AGGGTCTCTG GTGGAGAAAC 
     3061 TGCCTGACAG CCCTGCCCTG GCCAAGAAGG CATTACTGGC GCTGAACCAT GGGCTGGACA 
     3121 AGGCCCACAG CCTGATGGAG CTGAGCCCCT CAGCCCCACC TGGTGGCTCT CCACATTTGG 
     3181 ATTCTTCCCG TTCTCACAGC CCCAGCTCCC CAGACCCAGA CACACCATCT CCAGTTGGGG 
     3241 ACAGCCGAGC CCTGCAAGCC AGCCGAAACA CACGCATTCC CCACCTGGCT GGCAAGAAGG 
     3301 CTGTGGCTGA GGAGGATAAT GGCTCTATTG GCGAGGAAAC AGACTCCAGC CCAGGCCGGA 
     3361 AGAAGTTTCC CCTCAAAATC TTTAAGAAGC CTCTTAAGAA GACGCGTACG CGGCCGCTCG 
     3421 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3481 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3541 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3601 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3661 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3721 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3781 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3841 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3901 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3961 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4021 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4081 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4141 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4201 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4261 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4321 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4381 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4441 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4501 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4561 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4621 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4681 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4741 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4801 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4861 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4921 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4981 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5041 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5101 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5161 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5221 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5281 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5341 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5401 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5461 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5521 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5581 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5641 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5701 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5761 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5821 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5881 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5941 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6001 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6061 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6121 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6181 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6241 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6301 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6361 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6421 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6481 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6541 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6601 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6661 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6721 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6781 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6841 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6901 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6961 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7021 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7081 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7141 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7201 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//