LOCUS       RC239558                7236 bp    DNA     circular  SYN 
DEFINITION  ROBO2 (myc-DDK-tagged) - Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 5
ACCESSION   NM_001290065
VERSION     NM_001290065.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3393..3484 
                     /label="Myc-DDK"  
     misc_feature    3514..4099 
                     /label="PolyA signal"  
     misc_feature    3571..3590 
                     /label="Restriction Enzyme"  
     misc_feature    4375..5047 
                     /label="ColE1"  
     misc_feature    5477..6271 
                     /label="Kan/Neo"  
     misc_feature    6306..6608 
                     /label="SV40 ori"  
     misc_feature    6694..7171 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3375..3381 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3375 
                     /label="ROBO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCAGAGCG ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG 
     1081 ATCCTGTGCG CACACAAGAT ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC 
     1141 AGAAAGAGCT AGGAGATGTC CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA 
     1201 CGGTTCAGGT CACATGGACG GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA 
     1261 TGTATCGTCA GACTTCAGGT CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG 
     1321 TCCCGACTGA ACGAAGTGCT GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA 
     1381 AAGTACGGCC ATATTTTAAT GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA 
     1441 CTACTGAAGA AGCCCCAAGT GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT 
     1501 ACAATAGCAC AAGTATTAGT GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA 
     1561 TTATCCAAGA ATACAAGATC TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA 
     1621 CTGTGGATGC AGCCATTCGG TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC 
     1681 GGGTAGAGGT TGCAGCTAGT ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA 
     1741 TAATAATCGG GAGACGCAAT GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC 
     1801 AAATCACTGA TGTGGTGAAG CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG 
     1861 TAATTCTGAT GGGTTTTAGC ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA 
     1921 GTAATTATGC TGTTACGTTT CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC 
     1981 CAGGTCTTCT CAATGCTGGT GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA 
     2041 CGAGCTTGCC AGTAAATAAT AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG 
     2101 GAGATGTGCT GCCACCAGTT CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG 
     2161 GAGCCATTTA TAGTAGCATT GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA 
     2221 TAACACAGGC TACCCCATAT GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT 
     2281 TGGCTGTCGA TCTGCCTGAT CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA 
     2341 TGGGATTTGG TTATTCTCTA CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA 
     2401 AAAAGAAGAA AAATAAAAAC TCTTCTAAAC CACAGAAAAA CAATGGATCC ACTTGGGCCA 
     2461 ATGTCCCTCT ACCTCCCCCC CCAGTCCAGC CCCTTCCTGG CACGGAGCTG GAACACTATG 
     2521 CAGTGGAACA ACAAGAAAAT GGCTATGACA GTGATAGCTG GTGCCCACCA TTGCCAGTAC 
     2581 AAACTTACTT ACACCAAGGT CTGGAAGATG AACTGGAAGA AGATGATGAT AGGGTCCCAA 
     2641 CACCTCCTGT TCGAGGCGTG GCTTCTTCTC CTGCTATCTC CTTTGGACAG CAGTCCACTG 
     2701 CAACTCTTAC TCCATCCCCA CGGGAAGAGA TGCAACCCAT GCTGCAGGCT CACCTGGATG 
     2761 AGTTGACAAG AGCCTATCAG TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA 
     2821 ATCAACCTCC ACAGCCTCCA GTTCCACCGT TAGGTTATGT GTCTGGAGCC TTGATTTCTG 
     2881 ATTTGGAAAC GGATGTTGCA GATGATGATG CCGACGACGA AGAGGAAGCT TTAGAAATCC 
     2941 CCAGGCCCCT GAGAGCACTG GACCAGACTC CTGGATCCAG CATGGACAAT CTAGACAGCT 
     3001 CTGTGACAGG AAAAGCCTTT ACCTCCTCTC AAAGACCTCG ACCTACCAGC CCATTTTCTA 
     3061 CTGACAGTAA CACCAGTGCA GCCCTGAGTC AAAGTCAGAG GCCTCGGCCC ACTAAAAAAC 
     3121 ACAAGGGAGG GCGGATGGAC CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG 
     3181 ATGAGGAGGC CTTGGTGCCC TATAGCAAGC CCAGTTTCCC ATCTCCAGGT GGCCACAGCT 
     3241 CATCAGGAAC AGCTTCTTCT AAGGGATCCA CTGGACCTAG GAAAACCGAG GTGTTGAGAG 
     3301 CAGGCCACCA GCGCAATGCC AGCGACCTTC TTGACATAGG ATATATGGGC TCCAACAGTC 
     3361 AAGGACAGTT TACAGGTGAA TTAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3421 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3481 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3541 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3601 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3661 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3721 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3781 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3841 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3901 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3961 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4021 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4081 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4141 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4201 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4261 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4321 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4381 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4441 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4501 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4561 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4621 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4681 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4741 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4801 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4861 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4921 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4981 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5041 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5101 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5161 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5221 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5281 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5341 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5401 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5461 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5521 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5581 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5641 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5701 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5761 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5821 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5881 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5941 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6001 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6061 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6121 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6181 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6241 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6301 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6361 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6421 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6481 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6541 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6601 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6661 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6721 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6781 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6841 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6901 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6961 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7021 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7081 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7141 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7201 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//