LOCUS       RC239465                7119 bp    DNA     circular  SYN 
DEFINITION  ITGB6 (myc-DDK-tagged) - Human integrin, beta 6 (ITGB6), transcript variant 5
ACCESSION   NM_001282388
VERSION     NM_001282388.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3276..3367 
                     /label="Myc-DDK"  
     misc_feature    3397..3982 
                     /label="PolyA signal"  
     misc_feature    3454..3473 
                     /label="Restriction Enzyme"  
     misc_feature    4258..4930 
                     /label="ColE1"  
     misc_feature    5360..6154 
                     /label="Kan/Neo"  
     misc_feature    6189..6491 
                     /label="SV40 ori"  
     misc_feature    6577..7054 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3258..3264 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3258 
                     /label="ITGB6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCACGTAC AAGAATTTTA CTCATCCATC TGGAGTTGGC GAAAGGTGTG 
     1081 ATACCCCAGC AAACCTTTTA GCTAAAGGAT GTCAATTAAA CTTCATCGAA AACCCTGTCT 
     1141 CCCAAGTAGA AATACTTAAA AATAAGCCTC TCAGTGTAGG CAGACAGAAA AATAGTTCTG 
     1201 ACATTGTTCA GATTGCGCCT CAAAGCTTGA TCCTTAAGTT GAGACCAGGT GGTGCGCAGA 
     1261 CTCTGCAGGT GCATGTCCGC CAGACTGAGG ACTACCCGGT GGATTTGTAT TACCTCATGG 
     1321 ACCTCTCCGC CTCCATGGAT GACGACCTCA ACACAATAAA GGAGCTGGGC TCCCGGCTTT 
     1381 CCAAAGAGAT GTCTAAATTA ACCAGCAACT TTAGACTGGG CTTCGGATCT TTTGTGGAAA 
     1441 AACCTGTATC CCCTTTTGTG AAAACAACAC CAGAAGAAAT TGCCAACCCT TGCAGTAGTA 
     1501 TTCCATACTT CTGTTTACCT ACATTTGGAT TCAAGCACAT TTTGCCATTG ACAAATGATG 
     1561 CTGAAAGATT CAATGAAATT GTGAAGAATC AGAAAATTTC TGCTAATATT GACACACCCG 
     1621 AAGGTGGATT TGATGCAATT ATGCAAGCTG CTGTGTGTAA GGAAAAAATT GGCTGGCGGA 
     1681 ATGACTCCCT CCACCTCCTG GTCTTTGTGA GTGATGCTGA TTCTCATTTT GGAATGGACA 
     1741 GCAAACTAGC AGGCATCGTC ATTCCTAATG ACGGGCTCTG TCACTTGGAC AGCAAGAATG 
     1801 AATACTCCAT GTCAACTGTC TTGGAATATC CAACAATTGG ACAACTCATT GATAAACTGG 
     1861 TACAAAACAA CGTGTTATTG ATCTTCGCTG TAACCCAAGA ACAAGTTCAT TTATATGAGA 
     1921 ATTACGCAAA ACTTATTCCT GGAGCTACAG TAGGTCTACT TCAGAAGGAC TCCGGAAACA 
     1981 TTCTCCAGCT GATCATCTCA GCTTATGAAG AACTGCGGTC TGAGGTGGAA CTGGAAGTAT 
     2041 TAGGAGACAC TGAAGGACTC AACTTGTCAT TTACAGCCAT CTGTAACAAC GGTACCCTCT 
     2101 TCCAACACCA AAAGAAATGC TCTCACATGA AAGTGGGAGA CACAGCTTCC TTCAGCGTGA 
     2161 CTGTGAATAT CCCACACTGC GAGAGAAGAA GCAGGCACAT TATCATAAAG CCTGTGGGGC 
     2221 TGGGGGATGC CCTGGAATTA CTTGTCAGCC CAGAATGCAA CTGCGACTGT CAGAAAGAAG 
     2281 TGGAAGTGAA CAGCTCCAAA TGTCACCACG GGAACGGCTC TTTCCAGTGT GGGGTGTGTG 
     2341 CCTGCCACCC TGGCCACATG GGGCCTCGCT GTGAGTGTGG CGAGGACATG CTGAGCACAG 
     2401 ATTCCTGCAA GGAGGCCCCA GATCATCCCT CCTGCAGCGG AAGGGGTGAC TGCTACTGTG 
     2461 GGCAGTGTAT CTGCCACTTG TCTCCCTATG GAAACATTTA TGGGCCTTAT TGCCAGTGTG 
     2521 ACAATTTCTC CTGCGTGAGA CACAAAGGGC TGCTCTGCGG AGGTAACGGC GACTGTGACT 
     2581 GTGGTGAATG TGTGTGCAGG AGCGGCTGGA CTGGCGAGTA CTGCAACTGC ACCACCAGCA 
     2641 CGGACTCCTG CGTCTCTGAA GATGGAGTGC TCTGCAGCGG GCGCGGGGAC TGTGTTTGTG 
     2701 GCAAGTGTGT TTGCACAAAC CCTGGAGCCT CAGGACCAAC CTGTGAACGA TGTCCTACCT 
     2761 GTGGTGACCC CTGTAACTCT AAACGGAGCT GCATTGAGTG CCACCTGTCA GCAGCTGGCC 
     2821 AAGCCCGAGA AGAATGTGTG GACAAGTGCA AACTAGCTGG TGCGACCATC AGTGAAGAAG 
     2881 AAGATTTCTC AAAGGATGGT TCTGTTTCCT GCTCTCTGCA AGGAGAAAAT GAATGTCTTA 
     2941 TTACATTCCT AATAACTACA GATAATGAGG GGAAAACCAT CATTCACAGC ATCAATGAAA 
     3001 AAGATTGTCC GAAGCCTCCA AACATTCCCA TGATCATGTT AGGGGTTTCC CTGGCTATTC 
     3061 TTCTCATCGG GGTTGTCCTA CTGTGCATCT GGAAGCTACT GGTGTCATTT CATGATCGTA 
     3121 AAGAAGTTGC CAAATTTGAA GCAGAACGAT CAAAAGCCAA GTGGCAAACG GGAACCAATC 
     3181 CACTCTACAG AGGATCCACA AGTACTTTTA AAAATGTAAC TTATAAACAC AGGGAAAAAC 
     3241 AAAAGGTAGA CCTTTCCACA GATTGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3301 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3361 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3421 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3481 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3541 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3601 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3661 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3721 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3781 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3841 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3901 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3961 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4021 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4081 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4141 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4201 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4261 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4321 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4381 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4441 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4501 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4561 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4621 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4681 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4741 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4801 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4861 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4921 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4981 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5041 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5101 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5161 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5221 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5281 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5341 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5401 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5461 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5521 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5581 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5641 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5701 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5761 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5821 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5881 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5941 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6001 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6061 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6121 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6181 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6241 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6301 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6361 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6421 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6481 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6541 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6601 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6661 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6721 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6781 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6841 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6901 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6961 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7021 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7081 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//