LOCUS       RC239422                7053 bp    DNA     circular  SYN 
DEFINITION  NPC1L1 (myc-DDK-tagged) - Human NPC1-like 1 (NPC1L1), transcript variant 3
ACCESSION   NM_001300967
VERSION     NM_001300967.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3210..3301 
                     /label="Myc-DDK"  
     misc_feature    3331..3916 
                     /label="PolyA signal"  
     misc_feature    3388..3407 
                     /label="Restriction Enzyme"  
     misc_feature    4192..4864 
                     /label="ColE1"  
     misc_feature    5294..6088 
                     /label="Kan/Neo"  
     misc_feature    6123..6425 
                     /label="SV40 ori"  
     misc_feature    6511..6988 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3192..3198 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3192 
                     /label="NPC1L1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGGCC GGCCTGAGGG GCTGGCTGCT GTGGGCCCTG CTCCTGCGCT 
     1081 TGGCCCAGAG TGAGCCTTAC ACAACCATCC ACCAGCCTGG CTACTGCGCC TTCTATGACG 
     1141 AATGTGGGAA GAACCCAGAG CTGTCTGGAA GCCTCATGAC ACTCTCCAAC GTGTCCTGCC 
     1201 TGTCCAACAC GCCGGCCCGC AAGATCACAG GTGATCACCT GATCCTATTA CAGAAGATCT 
     1261 GCCCCCGCCT CTACACCGGC CCCAACACCC AAGCCTGCTG CTCCGCCAAG CAGCTGGTAT 
     1321 CACTGGAAGC GAGTCTGTCG ATCACCAAGG CCCTCCTCAC CCGCTGCCCA GCCTGCTCTG 
     1381 ACAATTTTGT GAACCTGCAC TGCCACAACA CGTGCAGCCC CAATCAGAGC CTCTTCATCA 
     1441 ATGTGACCCG CGTGGCCCAG CTAGGGGCTG GACAACTCCC AGCTGTGGTG GCCTATGAGG 
     1501 CCTTCTACCA GCATAGCTTT GCCGAGCAGA GCTATGACTC CTGCAGCCGT GTGCGCGTCC 
     1561 CTGCAGCTGC CACGCTGGCT GTGGGCACCA TGTGTGGCGT GTATGGCTCT GCCCTTTGCA 
     1621 ATGCCCAGCG CTGGCTCAAC TTCCAGGGAG ACACAGGCAA TGGTCTGGCC CCACTGGACA 
     1681 TCACCTTCCA CCTCTTGGAG CCTGGCCAGG CCGTGGGGAG TGGGATTCAG CCTCTGAATG 
     1741 AGGGGGTTGC ACGTTGCAAT GAGTCCCAAG GTGACGACGT GGCGACCTGC TCCTGCCAAG 
     1801 ACTGTGCTGC ATCCTGTCCT GCCATAGCCC GCCCCCAGGC CCTCGACTCC ACCTTCTACC 
     1861 TGGGCCAGAT GCCGGGCAGT CTGGTCCTCA TCATCATCCT CTGCTCTGTC TTCGCTGTGG 
     1921 TCACCATCCT GCTTGTGGGA TTCCGTGTGG CCCCCGCCAG GGACAAAAGC AAGATGGTGG 
     1981 ACCCCAAGAA GGGCACCAGC CTCTCTGACA AGCTCAGCTT CTCCACCCAC ACCCTCCTTG 
     2041 GCCAGTTCTT CCAGGGCTGG GGCACGTGGG TGGCTTCGTG GCCTCTGACC ATCTTGGTGC 
     2101 TATCTGTCAT CCCGGTGGTG GCCTTGGCAG CGGGCCTGGT CTTTACAGAA CTCACTACGG 
     2161 ACCCCGTGGA GCTGTGGTCG GCCCCCAACA GCCAAGCCCG GAGTGAGAAA GCTTTCCATG 
     2221 ACCAGCATTT CGGCCCCTTC TTCCGAACCA ACCAGGTGAT CCTGACGGCT CCTAACCGGT 
     2281 CCAGCTACAG GTATGACTCT CTGCTGCTGG GGCCCAAGAA CTTCAGCGGA ATCCTGGACC 
     2341 TGGACTTGCT GCTGGAGCTG CTAGAGCTGC AGGAGAGGCT GCGGCACCTC CAGGTATGGT 
     2401 CGCCCGAAGC ACAGCGCAAC ATCTCCCTGC AGGACATCTG CTACGCCCCC CTCAATCCGG 
     2461 ACAATACCAG TCTCTACGAC TGCTGCATCA ACAGCCTCCT GCAGTATTTC CAGAACAACC 
     2521 GCACGCTCCT GCTGCTCACA GCCAACCAGA CACTGATGGG GCAGACCTCC CAAGTCGACT 
     2581 GGAAGGACCA TTTTCTGTAC TGTGCCAATG CCCCGCTCAC CTTCAAGGAT GGCACAGCCC 
     2641 TGGCCCTGAG CTGCATGGCT GACTACGGGG CCCCTGTCTT CCCCTTCCTT GCCATTGGGG 
     2701 GGTACAAAGG AAAGGACTAT TCTGAGGCAG AGGCCCTGAT CATGACGTTC TCCCTCAACA 
     2761 ATTACCCTGC CGGGGACCCC CGTCTGGCCC AGGCCAAGCT GTGGGAGGAG GCCTTCTTAG 
     2821 AGGAAATGCG AGCCTTCCAG CGTCGGATGG CTGGCATGTT CCAGGTCACG TTCATGGCTG 
     2881 AGCGCTCTCT GGAAGACGAG ATCAATCGCA CCACAGCTGA AGACCTGCCC ATCTTTGCCA 
     2941 CCAGCTACAT TGTCATATTC CTGTACATCT CTCTGGCCCT GGGCAGCTAT TCCAGCTGGA 
     3001 GCCGAGTGAT GGTGGACTCC AAGGCCACGC TGGGCCTCGG CGGGGTGGCC GTGGTCCTGG 
     3061 GAGCAGTCAT GGCTGCCATG GGCTTCTTCT CCTACTTGGG TATCCGCTCC TCCCTGGTCA 
     3121 TCCTGCAAGT GGTTCCTTTC CTGGTGCTGT CCGTGGGGGC TGATAACATC TTCATCTTTG 
     3181 TTCTCGAGTA CCAGGGGCCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3241 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3301 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3361 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3421 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3481 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3541 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3601 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3661 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3721 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3781 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3841 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3901 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3961 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4021 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4081 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4141 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4201 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4261 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4321 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4381 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4441 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4501 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4561 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4621 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4681 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4741 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4801 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4861 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4921 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4981 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5041 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5101 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5161 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5221 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5281 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5341 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5401 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5461 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5521 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5581 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5641 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5701 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5761 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5821 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5881 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5941 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6001 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6061 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6121 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6181 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6241 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6301 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6361 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6421 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6481 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6541 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6601 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6661 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6721 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6781 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6841 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6901 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6961 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7021 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//