LOCUS       RC239368                6996 bp    DNA     circular  SYN 
DEFINITION  EOMES (myc-DDK-tagged) - Human eomesodermin (EOMES), transcript variant 1
ACCESSION   NM_001278182
VERSION     NM_001278182.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3153..3244 
                     /label="Myc-DDK"  
     misc_feature    3274..3859 
                     /label="PolyA signal"  
     misc_feature    3331..3350 
                     /label="Restriction Enzyme"  
     misc_feature    4135..4807 
                     /label="ColE1"  
     misc_feature    5237..6031 
                     /label="Kan/Neo"  
     misc_feature    6066..6368 
                     /label="SV40 ori"  
     misc_feature    6454..6931 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3135..3141 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3135 
                     /label="EOMES"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTTAGGG GAGCAGCTCT TGGTGAGCTC AGTGAACCTG CCTGGCGCGC 
     1081 ACTTCTACCC GCTGGAGAGT GCGCGAGGCG GCAGCGGCGG GAGCGCTGGC CACCTCCCCA 
     1141 GCGCGGCCCC CTCTCCTCAG AAGTTGGACT TAGACAAAGC GTCCAAGAAG TTTTCCGGCA 
     1201 GTCTCTCCTG CGAGGCGGTG AGCGGGGAGC CCGCAGCCGC CAGCGCAGGG GCCCCCGCGG 
     1261 CCATGCTTAG TGACACCGAC GCCGGGGACG CATTTGCCAG CGCTGCGGCA GTGGCCAAGC 
     1321 CGGGGCCCCC GGACGGCCGC AAGGGCTCCC CCTGCGGGGA GGAGGAGCTG CCCTCCGCCG 
     1381 CTGCAGCCGC CGCCGCCGCC GCCGCCGCGG CTGCGGCCAC TGCGCGCTAC TCCATGGACA 
     1441 GCCTGAGCTC CGAGCGGTAC TACCTCCAGT CCCCCGGTCC TCAGGGGTCG GAGCTGGCTG 
     1501 CGCCCTGCTC ACTCTTCCCG TACCAGGCGG CGGCTGGGGC GCCCCACGGA CCTGTGTACC 
     1561 CGGCTCCTAA CGGGGCGCGC TACCCCTACG GCTCCATGCT GCCCCCCGGC GGCTTCCCCG 
     1621 CGGCTGTGTG CCCACCCGGG AGGGCGCAGT TCGGCCCAGG AGCCGGTGCG GGCAGTGGCG 
     1681 CGGGCGGTAG CAGCGGCGGG GGCGGCGGCC CGGGCACCTA TCAGTACAGC CAGGGGGCTC 
     1741 CGCTCTACGG GCCGTACCCT GGAGCCGCAG CGGCGGGATC TTGCGGAGGA CTGGGGGGCC 
     1801 TGGGGGTTCC AGGTTCTGGC TTCCGTGCCC ACGTCTACCT GTGCAACCGG CCTCTGTGGC 
     1861 TCAAATTCCA CCGCCACCAA ACTGAGATGA TCATTACGAA ACAGGGCAGG CGCATGTTTC 
     1921 CTTTCTTGAG CTTCAACATA AACGGACTCA ATCCCACTGC CCACTACAAT GTGTTCGTAG 
     1981 AGGTGGTGCT GGCGGACCCC AACCACTGGC GCTTCCAGGG GGGCAAATGG GTGACCTGTG 
     2041 GCAAAGCCGA CAATAACATG CAGGGCAACA AAATGTATGT TCACCCAGAG TCTCCTAATA 
     2101 CTGGTTCCCA CTGGATGAGA CAGGAGATTT CATTCGGGAA ATTAAAACTC ACCAATAACA 
     2161 AAGGCGCAAA TAACAACAAC ACCCAGATGA TAGTCTTACA ATCCTTACAC AAATACCAAC 
     2221 CCCGACTGCA TATTGTTGAA GTTACAGAGG ATGGCGTGGA GGACTTGAAT GAGCCCTCAA 
     2281 AGACCCAGAC TTTTACCTTC TCAGAAACGC AATTCATTGC AGTGACTGCC TACCAAAACA 
     2341 CCGATATTAC TCAACTAAAG ATTGATCATA ACCCCTTTGC AAAAGGCTTC AGAGACAACT 
     2401 ATGATTCCAT GTACACCGCT TCAGAAAATG ACAGGTTAAC TCCATCTCCC ACGGATTCTC 
     2461 CTAGATCCCA TCAGATTGTC CCTGGAGGTC GGTACGGCGT TCAATCCTTC TTCCCGGAGC 
     2521 CCTTTGTCAA CACTTTACCT CAAGCCCGCT ATTATAATGG CGAGAGAACC GTGCCACAGA 
     2581 CCAACGGCCT CCTTTCACCC CAACAGAGCG AAGAGGTGGC CAACCCTCCC CAGCGGTGGC 
     2641 TTGTCACGCC TGTCCAGCAA CCTGGGACCA ACAAACTAGA CATCAGTTCC TATGAATCTG 
     2701 AATATACTTC TAGCACATTG CTCCCATATG GCATTAAATC CTTGCCCCTT CAGACATCCC 
     2761 ATGCCCTGGG GTATTACCCA GACCCAACCT TTCCTGCAAT GGCAGGGTGG GGAGGTCGAG 
     2821 GTTCTTACCA GAGGAAGATG GCAGCTGGAC TACCATGGAC CTCCAGAACA AGCCCCACTG 
     2881 TGTTCTCTGA AGATCAGCTC TCCAAGGAGA AAGTGAAAGA GGAAATTGGC TCTTCTTGGA 
     2941 TAGAGACACC CCCTTCCATC AAATCTCTAG ATTCCAATGA TTCAGGAGTA TACACCAGTG 
     3001 CTTGTAAGCG AAGGCGGCTG TCTCCTAGCA ACTCCAGTAA TGAAAATTCA CCCTCCATAA 
     3061 AGTGTGAGGA CATTAATGCT GAAGAGTATA GTAAAGACAC CTCAAAAGGC ATGGGAGGGT 
     3121 ATTATGCTTT TTACACAACT CCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3181 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3241 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3301 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3361 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3421 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3481 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3541 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3601 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3661 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3721 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3781 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3841 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3901 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3961 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4021 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4081 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4141 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4201 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4261 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4321 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4381 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4441 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4501 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4561 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4621 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4681 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4741 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4801 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4861 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4921 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4981 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5041 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5101 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5161 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5221 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5281 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5341 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5401 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5461 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5521 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5581 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5641 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5701 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5761 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5821 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5881 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5941 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6001 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6061 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6121 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6181 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6241 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6301 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6361 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6421 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6481 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6541 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6601 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6661 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6721 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6781 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6841 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6901 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6961 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//