LOCUS       RC235318                9480 bp    DNA     circular  SYN 
DEFINITION  KIAA0586 (Myc-DDK tagged) - Homo sapiens KIAA0586 (KIAA0586), transcript variant 2
ACCESSION   NM_001244190
VERSION     NM_001244190.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5637..5728 
                     /label="Myc-DDK"  
     misc_feature    5758..6343 
                     /label="PolyA signal"  
     misc_feature    5815..5834 
                     /label="Restriction Enzyme"  
     misc_feature    6619..7291 
                     /label="ColE1"  
     misc_feature    7721..8515 
                     /label="Kan/Neo"  
     misc_feature    8550..8852 
                     /label="SV40 ori"  
     misc_feature    8938..9415 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5619..5625 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5619 
                     /label="KIAA0586"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGTGAAG AGACTTCGTG AGGTAGTTTC TCAAAATCAT GGAGATCATT 
     1081 TGGTTTTGCT GAAAGATGAG TTGCCCTGTG TTCCTCCGGC ATTGTCTGCA AATAAACGTC 
     1141 TTCCTGTTGG AACGGGGACT AGTTTGAATG GAACATCACG TGGTTCATCA GACTTAACTT 
     1201 CTGCTAGAAA TTGTTACCAG CCTCTATTAG AAAATCCCAT GGTGTCAGAA AGTGATTTTT 
     1261 CTAAAGACGT TGCAGTGCAA GTGTTGCCTT TGGATAAAAT AGAAGAGAAC AACAAGCAAA 
     1321 AAGCAAATGA CATCTTCATT TCTCAGTATA CAATGGGACA GAAAGATGCT CTAAGAACAG 
     1381 TTTTAAAGCA AAAAGCTCAA AGCATGCCTG TTTTTAAGGA AGTAAAGGTA CATCTGTTAG 
     1441 AAGATGCAGG CATAGAGAAG GATGCTGTTA CTCAGGAGAC TAGAATTTCA CCCAGTGGAA 
     1501 TTGATTCAGC TACAACCGTG GCTGCAGCAA CTGCTGCTGC CATTGCAACC GCAGCTCCGT 
     1561 TGATAAAGGT GCAGAGTGAT TTGGAAGCAA AAGTCAATTC TGTTACAGAA TTACTTAGTA 
     1621 AATTACAGGA GACTGATAAA CACCTGCAAC GTGTTACAGA GCAGCAAACA AGCATTCAGA 
     1681 GGAAACAAGA GAAATTACAT TGTCATGATC ACGAAAAGCA AATGAATGTG TTTATGGAGC 
     1741 AGCACATAAG GCATCTTGAA AAGTTACAAC AACAACAAAT AGATATTCAG ACTCATTTTA 
     1801 TTAGTGCTGC ACTCAAGACT AGTAGTTTTC AGCCTGTTAG TATGCCCTCC TCCAGAGCAG 
     1861 TGGAAAAGTA TTCCGTAAAA CCAGAACACC CTAATCTTGG TAGCTGTAAT CCATCTTTAT 
     1921 ATAACACATT TGCTTCCAAA CAAGCACCTT TAAAAGAAGT TGAAGATACG AGTTTTGATA 
     1981 AACAGAAATC TCCTTTGGAG ACACCAGCAC CTCGCAGATT TGCTCCTGTA CCTGTTTCAA 
     2041 GGGATGATGA ACTATCAAAG AGGGAAAATC TTTTGGAAGA AAAAGAAAAT ATGGAAGTGT 
     2101 CGTGTCACAG AGGAAATGTA AGACTATTGG AACAAATTTT GAATAATAAT GATTCTTTGA 
     2161 CAAGAAAAAG TGAATCATCA AACACCACCT CACTAACTAG GTCAAAAATA GGATGGACTC 
     2221 CTGAGAAAAC AAACAGATTT CCTTCCTGTG AAGAGCTAGA AACAACTAAA GTGACTATGC 
     2281 AGAAGTCTGA TGATGTTCTT CATGACCTTG GCCAAAAAGA GAAAGAAACA AATAGCATGG 
     2341 TCCAGCCAAA AGAATCTCTG AGTATGTTGA AGCTTCCAGA TCTTCCACAG AATTCTGTTA 
     2401 AGCTTCAAAC AACCAATACA ACAAGATCTG TATTGAAAGA TGCTGAGAAG ATTTTGAGAG 
     2461 GAGTACAAAA CAATAAAAAA GTACTTGAAG AAAACCTGGA AGCTATTATT CGTGCAAAAG 
     2521 ATGGAGCTGC CATGTATTCG CTTATCAATG CTTTATCTAC CAACAGAGAG ATGTCAGAGA 
     2581 AAATTAGGAT CAGAAAGACA GTGGATGAAT GGATTAAAAC TATTTCTGCA GAAATTCAGG 
     2641 ATGAACTGTC AAGAACAGAT TATGAACAAA AAAGATTTGA TCAGAAGAAT CAGAGAACCA 
     2701 AGAAAGGTCA GAATATGACT AAAGATATTA GAACCAACAC ACAAGATAAA ACTGTCAACA 
     2761 AATCTGTAAT TCCAAGAAAA CATTCTCAAA AGCAAATAGA AGAGCATTTT AGAAATCTAC 
     2821 CTATGAGGGG CATGCCTGCT TCAAGTTTAC AGAAAGAGAG AAAGGAAGGG CTTTTGAAAG 
     2881 CAACCACAGT AATACAAGAT GAAGATTATA TGTTACAAGT CTATGGAAAG CCAGTTTATC 
     2941 AGGGCCATCG AAGCACTCTT AAAAAAGGAC CATATCTCAG ATTTAATTCT CCATCTCCTA 
     3001 AGTCCAGACC ACAGAGACCA AAAGTAATAG AACGAGTTAA AGGCACTAAG GTAAAGTCAA 
     3061 TAAGAACACA GACTGACTTC TATGCAACAA AACCTAAGAA GATGGATTCT AAAATGAAAC 
     3121 ATTCTGTTCC TGTGTTACCT CATGGCGATC AGCAATATTT GTTCAGCCCA AGTAGAGAAA 
     3181 TGCCTACTTT TTCAGGTACA TTGGAAGGTC ATCTGATTCC TATGGCAATT CTTTTAGGAC 
     3241 AAACCCAAAG TAATAGTGAT ACCATGCCAC CTGCTGGAGT GATTGTCAGC AAGCCACACC 
     3301 CTGTAACTGT GACTACTTCT ATTCCTCCAT CATCTCGAAA AGTAGAAACT GGAGTAAAGA 
     3361 AACCTAACAT AGCCATTGTA GAAATGAAGT CAGAAAAAAA GGATCCTCCT CAGCTTACTG 
     3421 TGCAGGTATT ACCCAGTGTA GATATTGACA GCATTTCAAA TAGTAGTGCT GATGTCCTTT 
     3481 CACCTCTGTC TAGCCCCAAA GAAGCATCTC TTCCTCCTGT GCAAACTTGG ATAAAGACTC 
     3541 CAGAAATTAT GAAGGTAGAT GAAGAAGAGG TGAAGTTTCC AGGAACTAAC TTTGATGAAA 
     3601 TAATCGATGT CATACAGGAA GAAGAAAAAT GTGATGAAAT TCCAGACTCT GAACCAATTC 
     3661 TGGAGTTTAA CAGAAGTGTT AAAGCTGATT CTACAAAATA TAATGGTCCT CCATTTCCGC 
     3721 CAGTTGCTTC TACTTTTCAG CCCACTGCTG ATATTCTGGA TAAAGTAATT GAGAGAAAAG 
     3781 AAACACTGGA AAATAGCTTA ATTCAATGGG TAGAGCAAGA AATAATGTCA AGAATTATCT 
     3841 CTGGGCTCTT TCCAGTCCAG CAACAGATTG CACCTAGTAT CAGTGTTTCA GTCAGTGAGA 
     3901 CAAGTGAACC ACTGACTTCT GACATTGTGG AAGGAACAAG CAGTGGCGCC CTCCAGCTTT 
     3961 TTGTTGATGC TGGTGTTCCT GTGAACTCAA ATGTGATTAA ACATTTTGTT AACGAAGCTC 
     4021 TTGCTGAGAC CATTGCTGTC ATGCTGGGTG ACAGAGAAGC AAAGAAGCAA GGTCCTGTTG 
     4081 CTACAGGTGT TTCTGGGGAT GCTTCAACAA ATGAAACATA TTTGCCGGCA AGAGTGTGCA 
     4141 CCCCACTGCC TACCCCACAG CCTACGCCTC CTTGCTCACC TTCATCACCT GCTAAGGAGT 
     4201 GTGTTTTGGT AAAGACTCCA GATTCTTCTC CCTGTGATTC GGATCATGAT ATGGCTTTTC 
     4261 CTGTGAAAGA AATATGTGCT GAAAAAGGAG ATGATATGCC TGCCATCATG CTTGTTAATA 
     4321 CTCCAACAGT TACCCCTACT ACTACACCTC CTCCAGCGGC GGCAGTTTTT ACCCCAACTT 
     4381 TGTCAGATAT TTCCATTGAT AAATTGAAGG TATCAAGCCC AGAGCTTCCC AAGCCATGGG 
     4441 GTGATGGAGA CCTGCCACTG GAAGAAGAGA ACCCTAACTC ACCTCAAGAA GAACTTCATC 
     4501 CAAGAGCTAT TGTAATGTCT GTGGCTAAGG ATGAAGAACC AGAGAGTATG GATTTCCCTG 
     4561 CTCAGCCTCC ACCTCCAGAG CCAGTTCCCT TTATGCCATT TCCTGCCGGC ACCAAGGCCC 
     4621 CTTCCCCCTC ACAGATGCCA GGTTCTGATT CATCAACACT GGAGAGCACA TTGAGTGTTA 
     4681 CTGTCACTGA AACTGAAACT TTAGATAAAC CCATCTCTGA AGGAGAGATT TTATTTAGCT 
     4741 GTGGTCAAAA ATTGGCCCCC AAGATTTTAG AAGATATAGG ACTGTACCTG ACAAACCTTA 
     4801 ATGATAGCTT ATCCAGCACT CTGCATGATG CCGTTGAAAT GGAGGATGAT CCTCCTAGTG 
     4861 AAGGGCAAGT GATTAGGATG TCCCATAAAA AATTTCATGC AGATGCAATT CTTTCTTTTG 
     4921 CTAAACAAAA CCAGGAGTCA GCAGTTTCCC AGCAAGCAGT CTATCATTCA GAGGACTTGG 
     4981 AAAACAGTGT GGGTGAACTT AGTGAAGGAC AAAGACCCCA GCTAACAGCG GCAGCAGAGA 
     5041 ACATCTTAAT GGGACATTCT CTCTATATGC AGCCACCTGT CACTAATACA CAGTCTTTGG 
     5101 ATCAACAATG TGATCCTAAA CCATTATCTC GGCAATTTGA CACAGTTTCA GGTAGTATTT 
     5161 ATGAAGATTC ATGTGCTAGT CATGGTCCAA TGAGTTTGGG AGAATTGGAG TTGGAGCCAA 
     5221 ATTCTAAGCT GGTTCTTCCC ACAACACTTC TGACAGCACA AGAAAATGAT GTTAATTTAC 
     5281 CAGTAGCCGC TGAAGATTTT TCCCAGTACC AACTAAAGCA AAATCAGGAT GTTAAGCAAG 
     5341 TTGAACACAA ACCATCACAA AGTTACCTAC GTGTTAGAAA TAAATCTGAT ATTGCACCTT 
     5401 CACAGCAACA AGTTTCACCA GGTGATATGG ATCGGACACA AATTGAGCTT AATCCGTACC 
     5461 TCACATGTGT ATTTTCAGGT GGGAAAGCAG TGCCACTCTC CGCTTCACAG ATGCCCCCTG 
     5521 CCAAGATGTC AGTGATGCTG CCGTCAGTGA ACCTCGAGGA CTGCTCTCAG TCTCTGAGTC 
     5581 TCAGCACAAT GCAGGAGGAC ATGGAGTCTT CGGGGGCAGA TACCTTCACG CGTACGCGGC 
     5641 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5701 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5761 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5821 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5881 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5941 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     6001 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     6061 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     6121 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     6181 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     6241 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6301 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6361 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6421 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6481 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6541 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6601 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6661 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6721 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6781 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6841 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6901 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6961 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     7021 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     7081 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     7141 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     7201 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     7261 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7321 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7381 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7441 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7501 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7561 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7621 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7681 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7741 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7801 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7861 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7921 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7981 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     8041 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     8101 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     8161 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     8221 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     8281 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8341 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8401 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8461 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8521 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8581 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8641 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8701 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8761 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8821 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8881 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8941 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     9001 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     9061 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     9121 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     9181 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     9241 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9301 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9361 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9421 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//